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High-performance UV detection for capillary electropharesis

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Product News ISCO’S new & detector is available now to meet the challenges of high-performance capil- lary electrophoresis. Designed for on-column absorbance detection with capillaries down to 50 pm ID, it gives excellent resuhs at any wave- length from 190 to 360 nm. The key part of an HPCE system is the detec- tor-other components don’t require special design and can be readily purchased or fabri- cated. To find out how easily you can get star- ted in HPCE. calI (800)22&4250 today. Circle no. 71 for more information The Programmable Thermal Controller (PTC-100) nay be used with any sample requiring cyclic 0; sequential temperature incubations. Solid-state Peltier-junction heat pumps ensure that the set tem- aerature will be reached with an accuracy of better than t 1 “C and no overshoot. The overall range is O-l 00°C Nith a peak rate of change surpassing 45°C per min- tite. Microprocessor control allows up to 100 steps to be input with a cycling capacity of 10,000 times for any series of these steps. The PTC-100 may be ordered to hold either a g&well microtiter plate or 60 0.5 ml micro- centrifuge tubes. User-friendly software allows pro- grams to be entered by name while the 32 character LCD displays all parameters simultaneously. MJ Research, Inc., Kendall Square Box 363 Cambridge, MA 02142. Tel: (617) 354-0016 0 Sjt Products for Molecular Biology Circle no. 73 for more information HUMAN GROWH HORMONE TRANSIENT GENE EXPRESSIONSYSTEM This advanced mathod provldas a fast, sensitive and reproducible a- of transient gene ex- pression without cell destruction. The control of gene expression can now be investigated based on the simple immunological detection of hGH secreted by cells transiently transfected with plasmids containing reg ulatory regions of interest fused tc the structural region of the hGH gene. When these plssmids are transfected into mamma- lian cells, hGH is secreted into the medium and small aliquots are then assayed for hGH levels. Secreted hGH levels are directly proportional to mRNA levels, which are easily detected by standard techniques. The Al16gro’M HGH assay takes only minutes to set up and quantitative results are available after only 90 minutes of incu- bation time...no need to wait for an autoradiograph. There are three different plasmids presently available with this system to aid in your investigations. l p0GH: A vector containing hGH structural sequences inserted adjacent to a polylinker. l pTKGH: A thymidine kinase/hGH fusion plasmid. l pXGH5: A mouse metallothionein I/hGH fusion plasmid. For further details call 1-800-642-4057 ext. 558 or write Nichols lnstituts Dlsgnostlcs Worldway Postal Center, P.O. Box 92797 Los Angeles, CA 90009-2797 Circle no. 72 for more informatlon TissueCulture Kit C HumanMelauocytes The MelanoF’ack isacomplete tissueculture kit containing Normal Human Epidermal Melanocytes (NHEM), melano- cyte growth medium (MGM, an optimized serum-free growth medium) and all the reagentsnecessary for sub- culture. MelanoPack provides the researcher with the con- venience of cultured NHEM that are ready for useand reliable from one shipment to the next. Melanocytes, which havehistoricallybeen very difficult to culture, have far-reachingimplications in cancer research (e.g.melanoma) and in studies which assess the dermatological impact of ultra- violet radiation.ThemelanocytesinMelanoPackprovideanexcellent normal model for such studies. MGM is an optimized serum-freetissueculture medium which does not contain cholera toxin and promotes the growth of melanocytes resulting in a population doubling rateof 36 hours. The melanocytes in MelanoF’ack can double 35-40 times before senescence becomesevident. For more information contact: Clonetics Corporation 9620 Chesapeake Drive San Diego, CA 92123 th8tk8 (800) 852-5663 (619) 541-0086 (CA) FAX (619) 541-0823 Circle no. 74 for more information
Transcript
Page 1: High-performance UV detection for capillary electropharesis

Product News

ISCO’S new & detector is available now to meet the challenges of high-performance capil- lary electrophoresis. Designed for on-column absorbance detection with capillaries down to 50 pm ID, it gives excellent resuhs at any wave- length from 190 to 360 nm.

The key part of an HPCE system is the detec- tor-other components don’t require special design and can be readily purchased or fabri- cated. To find out how easily you can get star- ted in HPCE. calI (800)22&4250 today.

Circle no. 71 for more information

The Programmable Thermal Controller (PTC-100) nay be used with any sample requiring cyclic 0; sequential temperature incubations. Solid-state Peltier-junction heat pumps ensure that the set tem- aerature will be reached with an accuracy of better than t 1 “C and no overshoot. The overall range is O-l 00°C Nith a peak rate of change surpassing 45°C per min- tite. Microprocessor control allows up to 100 steps to be input with a cycling capacity of 10,000 times for any series of these steps. The PTC-100 may be ordered to hold either a g&well microtiter plate or 60 0.5 ml micro- centrifuge tubes. User-friendly software allows pro- grams to be entered by name while the 32 character LCD displays all parameters simultaneously.

MJ Research, Inc., Kendall Square Box 363 Cambridge, MA 02142. Tel: (617) 354-0016

0 Sjt Products for Molecular Biology

Circle no. 73 for more information

HUMAN GROWH HORMONE TRANSIENT GENE EXPRESSION SYSTEM

This advanced mathod provldas a fast, sensitive and reproducible a- of transient gene ex- pression without cell destruction.

The control of gene expression can now be investigated based on the simple immunological detection of hGH secreted by cells transiently transfected with plasmids containing reg ulatory regions of interest fused tc the structural region of the hGH gene. When these plssmids are transfected into mamma- lian cells, hGH is secreted into the medium and small aliquots are then assayed for hGH levels. Secreted hGH levels are directly proportional to mRNA levels, which are easily detected by standard techniques.

The Al16gro’M HGH assay takes only minutes to set up and quantitative results are available after only 90 minutes of incu- bation time...no need to wait for an autoradiograph.

There are three different plasmids presently available with this system to aid in your investigations.

l p0GH: A vector containing hGH structural sequences inserted adjacent to a polylinker.

l pTKGH: A thymidine kinase/hGH fusion plasmid. l pXGH5: A mouse metallothionein I/hGH fusion

plasmid. For further details call 1-800-642-4057 ext. 558 or write

Nichols lnstituts Dlsgnostlcs Worldway Postal Center, P.O. Box 92797 Los Angeles, CA 90009-2797

Circle no. 72 for more informatlon

Tissue Culture Kit C Human Melauocytes The MelanoF’ack is a complete tissue culture kit containing Normal Human Epidermal Melanocytes (NHEM), melano- cyte growth medium (MGM, an optimized serum-free growth medium) and all the reagents necessary for sub- culture. MelanoPack provides the researcher with the con- venience of cultured NHEM that are ready for use and reliable from one shipment to the next.

Melanocytes, which have historically been very difficult to culture, have far-reaching implications in cancer research (e.g. melanoma) and in studies which assess the dermatological impact of ultra- violet radiation. ThemelanocytesinMelanoPackprovideanexcellent normal model for such studies.

MGM is an optimized serum-free tissue culture medium which does not contain cholera toxin and promotes the growth of melanocytes resulting in a population doubling rate of 36 hours. The melanocytes in MelanoF’ack can double 35-40 times before senescence becomes evident.

For more information contact: Clonetics Corporation 9620 Chesapeake Drive San Diego, CA 92123

th8tk8 (800) 852-5663 (619) 541-0086 (CA) FAX (619) 541-0823

Circle no. 74 for more information

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