Higher Content Yeast Imaging and Data Processing

Richard Alexander

Stowers Microscopy Center

May 19, 2009

High Content Imaging Candidates

• Reproducible

• Segmentable

• Automatable– Acquisition– Analysis

High Content Imaging Candidates

• Reproducible

• Segmentable

• Automatable– Acquisition– Analysis

Control Plate Experimental Plate

High Content Imaging Candidates

• Reproducible

• Segmentable

• Automatable– Acquisition– Analysis

High Content Imaging Candidates

• Reproducible

• Segmentable

• Automatable– Acquisition– Analysis

High Content Imaging Candidates

• Reproducible

• Segmentable

• Automatable– Acquisition– Analysis

High Content Imaging Candidates

• Reproducible

• Segmentable

• Automatable– Acquisition– Analysis

High Content Imaging Candidates

• Reproducible

• Segmentable

• Automatable– Acquisition– Analysis

High Content Imaging Candidates

• Reproducible

• Segmentable

• Automatable– Acquisition– Analysis

Use VBA Code to…

• Group Colonies

• Classify Colonies

• Compare between control and experimental plates

Use VBA Code to…

• Group Colonies

• Classify Colonies

• Compare between control and experimental plates

Use VBA Code to…

• Group Colonies

• Classify Colonies

• Compare between control and experimental plates

A B C123

Summary

• Yeast Robot– Pin yeast plate

• Flatbed Scanner– Image yeast plate

• Axiovision– Automate Segmentation– Automate Data Acquisition

• Excel– Automate Data Processing

Acknowledgements

• Rong Li– Das Arupratan– Brian Slaughter

• Molecular Biology– Dan Bradford– Brian Sanderson