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Histology Portfolio
Flavia Nodarse MartinezBIO 230-01 Histology
Prof. Abdallah M. Matari
Content The cellMitosisEpithelial tissue Connective tissue
Blood smearsCartilage and bone
Muscle tissue Nervous tissue
The Cell
Elodea leaf w.m. Chloroplasts
40x 100xCell wall Cytoplasm
Giant Chromosomes
100xChromosome
400xCytoplasm Centromere
Dorsal Root ganglion Golgi apparatus sec. silverstain
100x 400xNucleus
Golgi apparatus
Nucleic Acid onion root tip Azure B Stain sec.
100x 400xNucleus Cell wallCytoplasm
Nucleolus
DNA in animal cells sec. Feulgen
100x 400xNucleus (N), nucleolus (n), mitochondria (M), plasma membrane (Pm), cytoplasm (C)
N
nM
C
Pm
Epidermal cells of Allium cepa (onion)
40xCell wall (cw), cytoplasm (c), nucleus (N)
100x
cw
c
N
N
Mitosis
Crayfish testis mitosis/meiosis IH sec.
100x
P – prophaseM – metaphaseA – anaphaseT – telophase
A➝ P➝
M➝
T➝
Crayfish testis mitosis/meiosis IH sec.
P – prophaseM – metaphaseA – anaphaseT – telophaseC – cytokinesis
400x
A➝
P➝
M➝
T➝ C➝
Mitosis Onion root tip IH l.s.
100x
P – prophaseM – metaphaseA – anaphaseT – telophase
M➝P➝
A➝ T➝
Mitosis Onion root tip IH l.s.
400x
P – prophaseM – metaphaseA – anaphaseT – telophase
P➝
A➝
M➝ T➝
Epithelial Tissue
Epithelium Squamous section
100x 400xSsSimple squamous epithelium (Ss), Lumen (L), Nuclei (N) LN
L
Epithelium Cuboidal section
100x 400xSimple cuboidal epithelium (Scu), Lumen (L), Nuclei (N)
L
Scu NScu
Epithelium Pseudostratified Columnar
(ciliated) section
100x 400xCilia (C), Pseudostratified columnar epithelium (PsC), Nuclei (N), Goblet cells (GC), Basal cells (BC)
GC
C
NPsC
BC
Epithelium simple columnar section
100x 400xSimple columnar epithelium (Sc), Nuclei (N), Lumen (L), Basement membrane (Bm), Goblet cell (GC)
Sc N
LBm
GC
Epithelium Stratified Squamous non-cornified
40x 100xStratified squamous non-keratinized epithelium (StS), Basal layer (BL), Lumen (L), Nuclei (N)
StS
BL
L
N
Epithelium Transitional section
100x
400xTransitional epithelium (T), Round apical cells (Ra), Lumen (L), Nuclei (N), Basement membrane (BM)
T
L
Ra
N
BM
Olfactory epithelium mammal sec. H&E
100x 400xPseudostratified columnar epithelium (PsC), Cilia (C), Goblet cell (GC), Basement membrane (BM), Connective tissue (CT), Nuclei (N), Lumen (L)
CT
L
N
CGCPsC
BM
Trachea ciliated epithelium c.s. H&E
100x 400xSimple columnar epithelium (SC), Cilia (C), Nuclei (N), Basement membrane (BM), Connective tissue (CT),Lumen (L)
SC
L
SC
C
N
BM
CT
Connective Tissue
Adipose tissue fat (lipid) stained, w.m.
40x 100xAdipocyte
Lipid inclusionAdipose tissue
Nuclei
Adipose tissue fat (lipid) stained, w.m.
40x 100xNuclei Adipocyte
Lipid inclusion
Areolar Tissue
100x 400xFibroblast
Elastic fibers
Collagen fibers
Ligament yellow elastic connective tissue c.s. & l.s. H&E
100x 400x
Elastic fibers
Palatine tonsil human sec. H&E
100x 400x
Palatine epithelium(stratified squamous epithelium)
Connective tissue
Reticular tissue sec. m&nfr
100x 400x
Reticular fibers
Tendon White fibrous connective tissue l.s.
100x 400x
Dense regular tissue Fibroblasts
Collagen fibers
Fibroblast nuclei
White Fibrous Tissue; Tendon (Human), l.s.
100x 400x
Fibroblast
Collagen fibers
Dense regular connective tissue
Blood Smears
Bird blood smear
400x 1000x
Nucleatederythrocytes
Leukocytes
Frog blood smear
100x 400x
Nucleatederythrocytes
Leukocytes
Nuclei
Reptile blood
100x 400x
Nucleated erythrocytes
Thrombocytes
Human blood smear WrightErythrocytes
100x 400xLymphocyte
MonocyteLeukocytes
Human blood smear Wright (continuation)
Platelet
400x
Neutrophil
Monocyte400x
Human blood smear Wright (continuation)
Basophil
Eosinophil400x 400x
Red bone marrow mammal section H&E
100x 400x
AdipocytesBone marrow
Haversian canal Osteocyte
EndosteumMegakaryocytes
Cartilage & Bone
Hyaline cartilage trachea H&E sec.
100x 400x
Isogenous group
Chondrocytes embeddedin lacunae
Fibrous & chondrogenic layersPerichondrium
Matrix
Hyaline cartilage xiphisternum H&E sec.
Isogenous group
100x 400xMatrix
Perichondrium containing chondroblasts Chondrocytes
Elastic cartilage epiglottis sec.
100x 400x
Perichondrium
Chondrocytes Elastic fibers
White fibro-cartilage intervertebral disc sec.
100x 400x
Collagen fibers
Chondrocytes
Bone decalcified l.s. H&E
100x 400xOsteocytes
Volkmann’s canal Periosteum
Bone dry ground human c.s.
100x 400xCanaliculi
LamellaeHaversian canal
Osteocytes
Osteons
Compact bone decalcified H&E c.s.
100x 400xVolkman’s canalHaversian canal
Osteon
Intramembranous ossification fetal skull sec.
40x 100xCalcified bone Ossification center
Osteocytes Osteoblasts
Osteogenesis endochondral ossification
l.s. mal
40x 100xTrabeculae
Bone marrow Epiphysis
Periosteum Proliferation zone
Hypertrophic zoneCalcification zone
Ossification zone
Muscle Tissue
Cardiac muscle sec. H&E
100x 400x
Cardiac muscletransverse section
Myofiber
Connective tissueNuclei
Skeletal muscle human c.s. & l.s.
100x 400xI band (light) A band (dark)Sarcomeres
Skeletal muscle fibers (longitudinal section)
Nuclei
Skeletal muscle l.s. IH
100x 400xI band (light) A band (dark)
SarcomereSkeletal muscle fiber (longitudinal section)
Skeletal muscle tongue c.s. & l.s. fibers H&E
100x 400xNuclei I band
(light)A band(dark)
SarcomereSkeletal muscle fibers (longitudinal section)Skeletal muscle fibers
(cross section)
Smooth muscle intestine c.s. & l.s.
100x
400x
Smooth muscle fibers(cross section)
Smooth muscle fibers(longitudinal section)
Nuclei
Striated Muscle Mammalian Tongue c.s.
mas
100x 400x
Skeletal muscle fibers(cross section)
Skeletal muscle fibers (longitudinal section)
Nuclei Sarcomere
Nervous Tissue
Cerebellum & choroid plexus sec. H&E
40x 100x
White matter
Grey matter
Purkinje cell layer
Molecular layerGranule celllayer
Pia matter
Cerebellum anti-neurofilament
cytoskeleton sec.
40x 100xWhite matter
Grey matterPia matter
Purkinje cell layer
Molecular layer
Cerebrum mammal sec. CV
40x 400xPia matter White matter
Grey matter
Neurons
Neuroglia
Motor nerve endings reptile w.m.
40x 100xMotor end plates Axon terminals
Myelinated nerveAxon
Skeletal muscle fibers
Neurofibrillae Nerve cells sec. Spinal cord
100x 400xNeurophil
Multipolar neurons
Neuroglia cells & fibers sec.
100x 400xNeuroglial cells
Neurons
Peripheral nerve myelin Osmic Acid
100x 400x Myelinated axon
Perineurium
Axons
Endoneurium
Retina mammal sec. H&E
100x
400x
Ganglion cells
1
2
3
(1) Nerve fibers to optic nerve, (2)Bipolar cells, (3) Rod and cone nuclei, (4) Rod and cone outer segments, (5) Pigment epithelium, (6) Choroid, (7) Sclera
4
56 7
Spinal cord central nervous tissue c.s. H&E
40x 100xWhite matter
Grey matterCentral canal
Ventral horn
Spinal cord multipolar motor neurons nerve tissue smear MB&P
AxonAxon hillock
Multipolar neuron
Cell body
DendritesNeuroglial cells
Nucleus 400x100x
Sympathetic ganglion sec. H&E
100x 400xNeurons
Satellite cells surrounding the neurons
Axons Schwann cells
Referenceshttps://micro.magnet.fsu.edu/optics/intelplay/
gallery/pages/brightfield/femurmammal.htmlhttp://legacy.owensboro.kctcs.edu/gcaplan/
anat/Notes/API%20Notes%20F%20Connective%20Tissues.htm
http://www.histology-world.com/photoalbum/displayimage.php?album=97&pid=1099
https://www.studyblue.com/notes/note/n/neurobiology-exam-i/deck/5218020