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Tissue Engineering Lab/ Faculty of Medicine Tissue & organ printing: the future in tissue engineering ? Hybrid bioprinting Biofabrication of complex or vascularized tissue Hybrid bioprinting Self-assembling spheroids Pre- processing Biofabrication Post- processing • 3D in vitro models • Organ-on-a-chip • Regenerative medicine Patient-specific biomimetic tissue analogues PhDs Mendy Minne – Prof. Heidi Declercq Contact: [email protected] ✓ Disease modeling ✓ Personalized medicine ✓ Drug screening Maturation phase I Maturation phase II Microtissues Smart biomaterials External stimuli References: Mironov V. et al. Biomaterials 2009; De Moor L. et al. Biofabrication 2018; De Moor L. et al. Annals of Biomedical Engineering 2019; Vercruysse C. et al. In preparation; Roosens A. et al. Journal of Tissue Engineering and Regenerative Medicine 2019; Colle J. et al. Journal of Materials Science-Materials in Medicine 2020 Microtissues • Non-adhesive agarose microwells • High-throughput • Pores 200-400 μm (2865-1585/microwell) • Uniform & printable size Microtissues Spheroid generation Tissue specific spheroids Cartilage Fibrocartilage Bone Valvular tissue Adipose tissue • Engineering of 3D living structures • Precise placing of different cells, extracellular matrix and biomolecules in 3D • Layer-by-layer additive manufacturing Bioprinting Smart biomaterials & external stimuli Ink • Bioinks with electroactive components • Tissue fusion permissive hydrogels • Patient-derived bioinks • 3D aligned guiding systems Cell modalities Bioink Tissues: • Repeating functional units • Self-organizing systems Vascularized spheroids Lineage-specific differentiation of stem & primary cells Self-assembling of endothelial & supporting cells ✓ Multi- and heterocellular spheroids ✓ Increasing complexity ✓ 3D mimick of tissue PDMS mold PDMS mold agarose microwell liquid agarose
![Bioprinting and Differentiation of Stem Cells...Molecules 2016, 21, 1188 2 of 23 tissue [6,7]. The factors involved in the 3D bioprinting of stem cells and their interplay as discussed](https://static.documents.pub/doc/80x56/5fe6a82305804164be3b2e9d/bioprinting-and-differentiation-of-stem-cells-molecules-2016-21-1188-2-of.jpg)
