ICP SOFTWARE QTEGRA

G A M A L A . H A M I D

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Thanks

To everyone who has helped us with support,

new books, hard/soft ware And over the internet

Special thanks for Thermo

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Contents

• Introduction

• Configurator

• Qtegra

Templates

Labbooks

• Getting started

INTRODUCTIO

N

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ICP or ICP-AES or ICP-OES

• It is a multi-element analysis technique

that will dissociate a sample into its

constituent atoms and ions and exciting

them to a higher energy level.

• Cause them to emit light at a characteristic

wavelength , which will be analyzing

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Qtegra ISDS

Qtegra is the main module of the

Thermo Scientific Qtegra Intelligent

Scientific Data Solution (ISDS) and is

used to design, start and stop the

measurements of ICP.

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Qtegra Compliance with 21 CFR Part 11

• Part 11 of Title 21 of the Code of Federal Regulations,

Electronic Records, Electronic Signatures (21 CFR Part 11)

includes the U.S. federal guidelines for storage and protection

of electronically stored data and the application of electronic

signatures.

• The new Qtegra ISDS software provides a wide range of

features which enable laboratories to operate within total

FDA compliance. These features include audit trails, support

for electronic signatures and tools for integrated data

managements.

CONFIGURATO

R

The Configurator tool contains all

tools necessary to configure and

adjust the Qtegra ISDS framework

for your laboratory

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Contents

1. Access Control Editor

2. Experiment Configurator

3. Report Editor

4. Settings

5. Standard Editor

6. Viewer Region

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1. Access Control Editor

• Allows the Administrator and Manager to

control the access permissions by granting

or denying access to the different programs

and applications of Qtegra ISDS.

• Configurator - Access control editor -

Select an item from Virtual instruments or

Virtual evaluations - Access mode - Click

to display the list of access rights

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2. Experiment Configurator

• The Experiment configurator applet of the

Configurator tool combines instrument

sets.

• Each combination is saved as specific

Configuration for later use in the Qtegra

tool when creating a Template.

• Generally, only the Administrator and the

Manager have full access to this module

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Enabling the Drain Flow Sensor

• The Drain Flow Sensor is a unique safety

and time saving device which uses a bubble

counter to check that at least one bubble

passes from the instrument spray chamber

drain every two minutes.

• This ensures that if the sample introduction

becomes blocked/sample tubing has

detached that valuable time and gas is not

wasted by continuing the run.

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3. Report Editor

• The Report editor is a tool allows you to

create new report templates or to edit existing

templates.

• Report editor determines the layout of the

reports.

• To create a new report template

Configurator - Report editor - create a new

Report template.

Enter a Report name - Configure the layout -

save the Report template.

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4. Settings

• The Settings applet of the

Configurator tool controls default

settings,

• Configurator – Setting - Select

Logging and click Show All.

Set the Value for Misc. to True

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5. Standard Editor “ Global standards”

• The Standard Editor is a tool gives

access to the standards database.

• New standard files, and internal

standard files are created here.

• Standard Editor shows a list of

standards on the left.

• On the right the associated elements

and their concentration in the

standard solution are displayed as well

as the periodic table.

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6. Viewer Region

• The Viewer region displays a list of log files,

such as messages, errors and warnings.

QTEGRA Qtegra ISDS is a configurable software

package for elemental analyses. It is a

true end-to-end solution for

workflow-driven analysis.

softw

are

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Contents

Qtegra user Interface

1) Dashboard Page

2) LabBooks Page

3) Templates Page

4) LabBook Query Page

5. File Manager Page

6. Help Page

7. Scheduler

8. Completed LabBooks

9. Log View

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Qtegra user Interface

• The Home Page It contains all

functions offered by Qtegra and gives

access to all existing Templates and

LabBooks.

• The pages for measurement, result

analysis, the management of files and

helpful links are also accessed via the

Home Page.

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1. Dashboard page

It shows on one page the instrument controls and main settings of the iCAP 7000.

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Interlocks

Interlocks

• Check the interlocks are all green and take

appropriate action if any are RED.

• When all interlocks are green click on the

“red ringed” Get ready icon on the

Dashboard Page.

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Plasma

• Inductively Coupled Argon Plasma using

radio frequency bands. Solid state RF

generator with power efficiency >78 %.

• Nominal Frequency: 27.12 MHz

• Plasma ignition and operation are fully

automated and pc controlled.

• Power Output: iCAP 7200 ICP-OES power is

pre-optimized at 1150 watts. iCAP 7400 ICP-

OES 750 - 1500 watts computer controlled in

steps of 25 watts. iCAP 7600 ICP-OES 750 -

1600 watts computer controlled in steps

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Get ready

• It helps to start the instrument, switches on the

plasma and waits for the instrument to warm up.

• The Get Ready icon shortly shows yellow until

the state changes to ready while the plasma is

switched on, When the warm-up starts the icon is

already green.

• After warm-up, the spectrometer optimization is

started.

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Spectrometer Optimization

• The Spectrometer Optimization function may be performed at any

time, and is a routine which ensures that the wavelengths are

correctly located on the detector.

• This routine will automatically run when the plasma is ignited.

• During this routine, the pump will stop and the nebulizer gas will

be turned off.

• NOTICE It is recommended that the Spectrometer Optimization

function is carried out prior to analysis.

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Nebulizer Optimization

• The Nebulizer Optimization optimizes the

nebulizer pressure settings for the

performance tests.

• NOTICE Standard concentric glass nebulizers

are optimized for a flow of approximately 0.7

to 1 L/min.

• Ensure that gas flows in excess of 1.0 L/min are

not used as this may result in sub-optimal

performance.

• Other nebulizers may run at higher flows.

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Performance test

• The performance checks are defined by the Sensitivity,

Relative Standard Deviation, and Detection Limit tests.

• The performance checks should be run using the

standard aqueous solutions kit.

• Once the performance checks are passed, the

instrument is ready for operation. If the performance

checks fail, check for causes and run the tests again.

• The Performance Check is followed by the Relative

Standard Deviation test and the Detection Limit.

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Passed or Failed

• When the procedure has been finished, the

display changes accordingly.

• The display shows Failed for tests that have

not been passed successfully,

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Torch Alignment

• For maximum sensitivity it is advisable to check the

alignment of the torch whenever it has been

removed and replaced in the instrument or if the

torch body or centre tube has been replaced.

• Four Alignment steps are performed.

Radial, slit=UV follow, slit=Vis,

Axial, slit=UV, and, slit=Vis.

• The 2 ppm Zinc Solution for Torch Alignment should

be made up with either an aqueous or an organic

matrix as required.

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Auto peak

• iCAP will automatically check a reference line each

time the plasma is ignited to maintain wavelength

accuracy.

• wavelengths are correctly aligned in the centre of

the sub array measurement window

• Only needs to be run whenever a new wavelength

is used for analysis or if the instrument has been

switched off for an extended period of time.

• Allow the plasma to stabilize for 10 minutes.

• Introduce the sample “Method development

standard” (high standard concentration solution of

the selected element(s).

Using the iCAP 7000 Test

Solution ( High Concentration Standard

Solution containing: 10ppm P, 5ppm K,

Ni; 1ppm Al, Mn, Cu and 0.2 ppm Zn,

Mg, Ca, Ba, 0.2% HNO3).

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Organic material

Organics Check box

To enable Auto peak to be performed with

organic standards.

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Closing Down the System

• The iCAP 7000 Series ICP-OES system can

be shut down with the Shut Down function

on the Dashboard page of Qtegra.

• The iCAP 7000 Series ICP-OES system shuts

down and the plasma switches off.

• The state of the Configuration changes to

Not Ready.

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The Post Ignition Defaults

• Post Ignition Defaults can be set in order to

immediately change start-up settings, all at

the same time.

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Changing the Configuration

• It is frequently necessary to change the

Configuration of the iCAP 7000 Series ICP-

OES instrument.

• With the Change Configuration function on

the Dashboard page of Qtegra you can

easily change the Configuration.

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Using autosampler

• If you run the tests using an autosampler, enter Wash time and Uptake time, as

well as Rack and Vial position for Multi Element Test Solution and Blank

solution.

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2. LabBooks Page

• On the LabBooks page of Qtegra,

LabBooks are created and opened.

• LabBooks are created from :

1. Blank Templates,

2. Existing Templates or from

3. Existing LabBooks .

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3. Templates Page

• The analytical workflow for sample

measurement is defined in a Template.

• select a template in recent template

• Or create a new template.

• Or open a saved template

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Template Sections

A Template contains all basic information on

analytes, Template consists of :-

• A Method Parameters section,

• A Sample Definition section,

• An Automatic Export section,

• And a Peripherals section.

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4. LabBook Query Page

• the location of the LabBooks and the

period of time measurements were done

and parse through the available results.

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5. File Manager Page

• Organize your Template and LabBook files.

• On the File Manager you can cut, copy, delete or rename your Template and

LabBook files

• The folder Performance Report gives access to the performance test results.

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6. Help Page

• provides information about Qtegra

ISDS, support and tools.

• In the Support section on the Help

page of Qtegra offers access to the

Qtegra ISDS Help.

• In the Tools section on the Help page

of Qtegra, you can customize your

Home Page settings.

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7. Scheduler

• In the Scheduler tool of Qtegra, the

measurement for a scheduled LabBook is

executed.

• The completed LabBook is automatically

deleted from the Scheduler and added to

“Completed LabBooks”

• To add a LabBook to the Scheduler

Qtegra - Open a LabBook - In the toolbar of

the LabBook, click to schedule the

LabBook.

The LabBook is added to the Scheduler

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8. Completed LabBooks

• Upon completion of a LabBook, the LabBook is automatically deleted from Scheduler and

added to the Completed LabBooks tab in Qtegra.

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9. Log View

• The Log View region of Qtegra displays a list of messages, such as errors and

warnings. By default, different types of messages are displayed.

• (The Viewer tab is also shown in “Configurator”

A TEMPLA

TE

The analytical workflow for

sample measurement

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Content

1. Method parameters

2. Sample definition

3. Automatic export

4. Peripherals section

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Evaluation

eQuant

• Uses external element concentrations to

quantify element concentrations in an

unknown sample.

Raw Data

• Displays the acquired raw intensities which

are then used by the different evaluations.

Method Parameters

• might differ, depending on the Evaluation

selected for the Template or LabBook in

Qtegra.

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Template tool bar

• Save,

• Close or run a Template,

• Create a new LabBook or Template from the existing current Template,

• View the history of the current Template,

• Hide the Content pane,

• Auto peak,

• Time Study, show transient signal during exposure.

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1. Method Parameters

a. Analytes

b. Measure Modes

c. Acquisition Parameters

d. Intelligent Uptake and Rinse

e. Inter-Element Correction

f. Standards

g. Quantification

h. Ratios

i. Quality Control

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a. Analytes

• The analytes to be acquired during the

measurement.

• Analytes can directly be selected from the

periodic table display

• For some elements, several analytical

wavelengths are displayed in descending

order of relative intensity.

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Lines interferences

• The details of the element are displayed

with symbol, wavelength, relative

intensity, state and known interferences as

stored in the element database

• The wavelength of the analyte is displayed

in the yellow graph

• The wavelength of the interference is

displayed in the red graph

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b. Measure Modes

• Parameters are defined in order to optimize

the analysis by the radial and/or axial

configurations.

• In the axial configuration, the plasma is viewed

end-on (along the length of the plasma). That

offers greater sensitivity.

• In the radial configuration, the plasma is

viewed from the side. The radial plasma view

offers less sensitivity

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Full frame

• With the Evaluation Results Full Frames view of the LabBook in Qtegra you can identify all

the elements present.

• Method elements are shown on the right in the Elements panel.

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c. Acquisition Parameters

Analysis Mode

Normal mode is the traditional analysis mode which is per replicate.

Speed analyzes all radial/high wavelength elements for all replicates, then all axial/low

wavelength elements for all replicates.

Sprint similar manner to Speed. This mode removes any overhead time associated with

the pre-exposure ensuring minimum sample analysis time.

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d. Intelligent Uptake and Rinse

• Delays for Uptake and Wash can be defined

for the analytes added to the table.

• The Uptake starts when the signal for the

specified value for the analyte or analytes is

stable.

• If this value falls below the specified value

after the measurement has been

completed, the Wash procedure starts.

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e. Inter-Element Correction

• The Inter-Element Correction is a

corrections for direct spectral

interferences.

• Select (Enable) for the element you

wish to define the correction for.

• Select the (Enable )for the

appropriate element and enter the

formula for the correction.

• Click to save the changes

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f. Standards

• Standards are materials containing a known

concentration of an analyte.

• They provide a reference to determine unknown

concentrations or to calibrate analytical

instruments.

• The Standards section is used to enter

information about each calibration standard that

will be used to generate the calibration curve.

• Concentration ratio standards and summation to

100% are for solid.

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Commands of the Standards

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g. Quantification

The Quantification view to set the calibration and quantification strategy for each analyte.

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Quantification

• Customized Traces feature allows converting an element concentration into a

molecule concentration.

• Multi-Calibration trace will enable switching from a sensitive wavelength to a less

sensitive wavelength depending on the measured concentration.

• Internal standard is used with warning limits and failure limits of its recovery in

percent.

• Standard Range To control the calibration range. Values outside this range are

excluded from calibration.

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h. Ratios

• Allows you to define analyte ratios for the measurement.

• Displays the ratio of columns Analyte 1 and Analyte 2.

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i. Quality Control

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Quality control blank tests

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Quality control calibration tests

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Quality control paired sample tests US EPA

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Quality control spike recovery US EPA

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Quality control spike recovery US EPA

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2. Sample definition

• Sample Definition is a sub-section in

the Template view in Qtegra.

• In this section the parameters of the

measurements are defined.

• The QC settings are specified for each

sample in the Sample Definition section

of the eQuant Template in Qtegra.

• QC samples can be defined as Initial

Actions, as Continuing Actions with the

appropriate value for Interval, or in

special cases as End Actions.

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3. Automatic export

In the Automatic Export view of a Template in Qtegra, you define the export settings for your

data as *.csv or *.xml file and for reports.

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Report Export

• Upon completion of the LabBook, the data are automatically exported as defined.

• The export directory is defined under System Options in the Scheduler settings in the

Export Directory

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4. Peripherals

• Peripherals are added to the

Configuration in the Configurator tool

of Qtegra.

• The settings for peripherals can be

adjusted in the corresponding view of

the Template or LabBook in Qtegra

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Manual Sample Control

• Manual Sample Control can be added to

your Configuration in the Configurator to

enter samples without autosampler.

• In the Manual Sample Control view in

Qtegra, Uptake and Wash Time can be

defined.

• Time mainly depends on the length of the

probe capillary and the uptake rate.

• The value of the Wash Time should be

increased when going for high analyte

concentrations or tough matrices to avoid

carry-over effects.

LABBOOKS

LabBooks contain all

information needed to run a

measurement in Qtegra

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Contents

1. LabBook Toolbar

2. Color Scheme of the Periodic

Table

3. Method Parameters LabBook

4. Summary of LabBook

5. Sample List - LabBook

6. Automatic Export - LabBook

7. Scheduling a LabBook

8. Viewing the Result of a Measurement

9. Log Messages

10. Signing

11. Query

12. Reports

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labBook Creation

• LabBooks are created from

Templates or LabBooks and are

based on the settings specified

in the respective Template or

LabBook.

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1. labBook toolbar

• Save,

• Close ,

• Run or export a LabBook,

• Unlock will only appear after the LabBook has been added to the Scheduler,

• Create a new LabBook or Template from the existing current labBook,

• Export LabBook data

• View the history of the current Template,

• Set the same order for the elements in all the tables,

• Hide the Content pane.

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2. Color Scheme of the Periodic Table

The color scheme of the periodic table of the LabBook is inherited from

the definitions in the Template.

A shortcut menu offers several color schemes for the periodic table

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3. Method Parameters LabBook

• Method Parameters are inherited from the

Template or LabBook from which the

LabBook is created in Qtegra.

• All settings of the Method Parameters can

be still be changed in the LabBook in

Qtegra.

• If you create a LabBook from a blank

Template, the Configuration currently

loaded on the Dashboard is used

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4. Summary of LabBook

A summary page is added to each LabBook in Qtegra.

This page shows the file name, and information about Properties

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5. Sample List

• The Sample List of a LabBook is

based on:-

• The number of samples selected

for analysis,

• The structure of the

o Initial Actions,

o Continuing Actions and

o End Actions items

defined in the section of the

Templates.

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6. Automatic Export

• Before measurement, you can define

Automatic Export settings for a LabBook in

Qtegra.

• Upon completion of the LabBook, the data

are automatically exported.

• LabBooks inherit the Automatic Export

settings from the Template.

• Automatic Export settings are not available

for Completed LabBooks since they have

already been exported if so defined.

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7. Scheduling a LabBook

• Click to schedule the LabBook.

• The LabBook is added to the Scheduler

• The completed LabBook is automatically

deleted from the Scheduler and added to

Completed LabBooks.

• If you wish to still change LabBook

parameters, click to unlock the

LabBook The LabBook is unlocked and

remove from the Scheduler

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8. Viewing the Result of a Measurement

• Recalculate Updates the data automatically, The button then flashes to advise you there are

some updates to be performed.

• Inter-Element Correction, Switches the mathematical interference correction on and off.

• Blank Correction Switches the Blank correction on and off.

• Use Internal Standard Switches the use of an internal standard on and off.

• Display Details Displays an enlarged graph of the calibration curve including the calculated

values for the background equivalent concentration (BEC), the instrumental detection limit

(IDL) and the most common statistical data.

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Results Intensity

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Sub-array

• During analysis, the CID detector is read in a

sub-array configuration.

• In order to reduce the read cycle time, all

analytical wavelengths are designated, as a

small array that, by default for UV is 5 pixels

tall by 12 pixels wide and for the VIS is 2

pixels high by 12 pixels wide.

• The maximum size for any Subarray is 24

pixels wide by 7 high.

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Sub-array functions

Figure shows the spectrum for a situation where an

interfering line is observed beside the element

of interest.

The analyst has many choices:

1. Select an alternate interferant free line.

2. Move or turn off the background points to

negate the interferant

3. Use an interfering element correction (IEC)

to counteract the effect of the interfering

element. This method is only applicable to

direct spectral overlaps .

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Results Concentration

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9. Log Messages

• The Log Messages view of a LabBook in

Qtegra is added to the LabBook after a

measurement has been run for this

LabBook.

• It contains all events with appropriate

time stamps which occur throughout the

manipulation of the LabBook. All

information, warning, error and service

concerned messages are logged.

• In the Completed LabBooks tab, click the

LabBook you wish to view

• Use filter in the header of the column

you wish to filter the display of.

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10. Signing

• The Signing view of a LabBook in Qtegra is

added to the LabBook after a measurement

has been run for this LabBook.

• SW provides a multi-user Signing workflow

for LabBooks.

• This workflow can be adapted to your

typical laboratory workflow. The default

Signing workflow provides the steps

Acquired By, Verified By, and Approved By.

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11. Query

• The Query view of a LabBook in

Qtegra is added to the LabBook

after a measurement has been run

for this LabBook.

• The Query and Reports view of a

completed LabBook are basically

the same as on the Results page of

a LabBook Query.

• An overview of different result

information like intensities or

determined concentration can be

displayed at a glance and can easily

be exported.

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12. Reports

• The Reports view of a completed LabBook

in Qtegra displays the reports of a

measurement in report formats previously

created.

• In the Reports view of a completed

LabBook in Qtegra, you generate result

data reports from presets.

• You can structure your report to your

needs.

GETTING STARTE

D

Analysis with eQuant Evaluation

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Steps

1. Setting Up the Template

2. Creating LabBook for Analysis with

eQuant Evaluation

3. Run the Experiment of your Analysis

with eQuant

4. Results and Data Evaluation

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1. Setting Up the Template

1- Create a template “ select and define the

required data and

parameters on next”:

a. Analytes

b. Measure Modes

c. Acquisition Parameters

d. Intelligent Uptake and Rinse

e. Inter-Element Correction

f. Standards

g. Quantification

h. Ratios

i. Quality Control (save the changes to your Template).

2- Define settings of autosampler.

Define the settings of your autosampler

as appropriate.

3- Define Sample Definition

Define Initial Actions, Continuing Actions

and End Actions as appropriate.

(save the changes to your Template).

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2. Creating LabBook for Analysis

• Qtegra - Home Page - click Analysis.

• Enter a Name for the LabBook and select a

Location.

• Click the radio button Create a new

LabBook from an existing Template.

• Enter a number for Samples.

• Import a sample list.

• In the toolbar of your LabBook page, click

to save your LabBook.

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Getting Started notes

• The instrument is designed to be constantly powered up and the optical system

continuously purged.

• The instrument is powered via an on/off switch at the rear of the left side

• If the instrument power has been switched off, allow at least two hours after restoring

power to thermally stabilize the instrument.

• If the gas supplies have been switched off, the optical components should be purged for at

least two hours before turning on the water chiller and running samples. Doing so can

seriously damage the CID detector.

• Deionized water (or organic solvent if using an organics kit) should be aspirated for twenty

minutes to allow the instrument to fully stabilize before analysis.

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Preparing the system

1. Turn Argon Gas on at Cylinder and set for 0.6 MPa (6 BAR) pressure

on gauge near instrument

2. NOTE: for normal use gas should be left purging constantly

3. Switch on power to iCAP 7000 Series ICP-OES Spectrometer.

4. NOTE: for normal use power should be left on constantly

5. Switch on Water Chiller

6. Push Platen on to rollers of pump by way of the 4 (3) pressure screws

7. Make sure the drain tube is placed in an open neck vessel

8. Place sample tube in a blank solution

9. Switch on computer

10. Click on the “Qtegra” Icon on desktop

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Continue

11. Purge boost button at rear right of instrument On/off switch at rear left of instrument

12. Camera temperature should be - 46 °C (±2)

13. Optics temperature should be 38 °C (±1)

14. Gas supplies - Ar plasma gas 5.5 bar (80 psi)

15. N purge gas (if used) 3-5.5 bar (45-80 psi)

16. Chiller temperature set to 5 °C below the room temperature (and must be set between 15 °C

and 30 °C).

17. Torch is correctly fitted in the torch holder and the torch holder is locked into the torch box.

18. Ensure adequate provision for waste sample is made.

19. Sample tubing, particularly pump tubing, should be in good condition.

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Lighting the plasma

• Click on “Qtegra” to open the program.

• On the Dashboard Page, click on the “Get

Ready” icon to open the window.

• Define warm up time. Click “ok”.

• NOTE: To allow the plasma to stabilize leave

Plasma on with blank solution running for

about 10 minutes before carrying out an

analysis.

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LED indicators

• On the rear right hand side of instrument

there are row of LED’s which indicate the

status of the instrument.

• Once the chiller has been turned on and

has reached its set temperature LED’s 2-7

should be on and led 1 and 8 should be

flashing.

• LED 9 indicates engineer fast purge has

been selected and should be t turned off

when analyzing samples.

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3. Run the Experiment of Analysis

• Qtegra - Home Page - Analysis.

• Below Open LabBook, click The Browse for

LabBook dialog opens - Select your eQuant

LabBook.

• Click to open the LabBook.

• The LabBook opens in a new tab of Qtegra.

• In the toolbar of your LabBook, click to

schedule the LabBook for execution.

• The LabBook is added to the Scheduler.

• NOTICE If the check box Automatic has

been selected for Start Queue in the

Options settings of the Scheduler (see

“Customizing Scheduler Settings”

the measurement starts immediately.

• If the execution does not start immediately,

click in the toolbar of the Scheduler. The

execution of the LabBook is started.

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Sampling

• If using the Manual Sample

Control define Uptake and

wash times.

• If using an Autosampler define

the autosampler settings.

• On the sample list define the

samples to be analyzed.

• If using an autosampler

identify the rack and vial for

each sample.

Gamal A. Hamid102

Running the analysis

• In the toolbar of your LabBook click “Run”

to schedule the LabBook for execution.

• The LabBook is added to the scheduler.

• If the check box Automatic has been

selected for Start Queue in the options

settings of the scheduler the measurement

is started immediately.

Gamal A. Hamid103

4. Results and Data Evaluation

• In the Evaluation Results Concentrations view of the

LabBook in Qtegra, the results of the quantitative

analysis are summarized.

• As with the sample list, blanks are displayed in blue,

standards in yellow, QCs in red and unknowns in

white.

• The mean values, standard deviations (SD) and

relative standard deviations (RSD) as well as the

results of each main run are shown when the line is

expanded by clicking.

Gamal A. Hamid104

Shutting Down the Instrument

• After an analysis is finished a blank sample should be aspirated for five minutes to ensure

the sample introduction components have been rinsed of sample. then deionized or

distilled water .

• For organic the final rinse should be the pure solvent. Air should be aspirated for one

minute to remove organic solvent from the sample lines and to remove organic vapors.

• The plasma should be turned off.

• Allow two minutes before switching off the water chiller and gas supplies.

• Allow five minutes after switching off the instrument, or accessories, before disconnecting

the electrical power or other supplies.

• Release the tension on the sample pump platens.

Thanks

gamal.abdelhamid@meslo.com.eggamal_a_hamid@hotmail.com