IDENTIFYING THE MELANOMA IDENTIFYING THE MELANOMA WAVELENGTHSWAVELENGTHS
Wavelength280 320 360 400
IRR
AD
IAN
CE
(W
/cm
2 /n
m)
1e-7
1e-6
1e-5
1e-4
1e-3
San Diego, CAJuly 4, 2000
UVAUVB
(nm)
WAVELENGTH (nm)
250 260 270 280 290 300 310 320 330
IRR
AD
IAN
CE
(W
/cm
2/n
m)
1e-7
1e-6
1e-5
1e-4
Wavelength (nm)
300 400 500 600 700 800 900
Irra
dia
nc
e (
W/c
m2/n
m)
1e-9
1e-8
1e-7
1e-6
1e-5
1e-4
Solar Simulator
Wavelength (nm)
250 300 350 400 450
Irra
dia
nc
e (
W/c
m2
/nm
)
1e-7
1e-6
1e-5
1e-4
UVA UVB
E. De Fabo, EOH, SPHHS, GWUMCE. De Fabo, EOH, SPHHS, GWUMC [email protected] [email protected] 202-994-3975202-994-3975
BLACK ALBINO
20/24(3.7)
6/18(1.0)
E. De Fabo, EOH, SPHHS, GWUMCE. De Fabo, EOH, SPHHS, GWUMC
Effect of pigment on melanomaEffect of pigment on melanoma
SPD PHILLIPS F40 SUNLAMPS (6)MEASURED BY OPTRONICS 742 SPECTRORADIOMETER
Wavelength (nm)
200 300 400 500 600 700 800 900
Irra
dia
nce
(W
/cm
2 /nm
)
1e-11
1e-10
1e-9
1e-8
1e-7
1e-6
1e-5
1e-4
1e-3
UVB UVA
C57BL6 HGF/SF
FVB HGF/SF
C57BL6 HGF/SF
STRAIN 1
STRAIN 2
FVB HGF/SF
E. De Fabo, EOH, SPHHS, GWUMCE. De Fabo, EOH, SPHHS, GWUMC De Fabo et al., Cancer Res., 2004; in prep.
GFP Induction 24 Hrs Post-DoxGFP Induction 24 Hrs Post-Dox R.Zaidi, NCI,NIHR.Zaidi, NCI,NIH
• Pups were injected doxycycline on the day of birth.Pups were injected doxycycline on the day of birth.
• Back skins were harvested 24 hours after the doxycycline injection Back skins were harvested 24 hours after the doxycycline injection
and imaged for GFP.and imaged for GFP.
Dct-rtTA– / GFP+ Dct-rtTA+ / GFP+
E. De Fabo, EOH, SPHHS, GWUMCE. De Fabo, EOH, SPHHS, GWUMC
UVB + ALBINO
STRAIN-1
UVA + PIGMENT
STRAIN-2
UVB + PIGMENT
STRAIN-2
UVA + ALBINO
STRAIN-1
E. De Fabo, EOH, SPHHS, GWUMCE. De Fabo, EOH, SPHHS, GWUMC
ACKNOWLEDGEMENTSACKNOWLEDGEMENTS• THE GEORGE WASHINGTON UNIVERSITY THE GEORGE WASHINGTON UNIVERSITY
SCHOOL OF PUBLIC HEALTH & HEALTH SERVICESSCHOOL OF PUBLIC HEALTH & HEALTH SERVICES
FRANCES NOONANFRANCES NOONAN
JESSE BAHN JESSE BAHN
SCHOOL OF MEDICINE & HEALTH SCIENCESSCHOOL OF MEDICINE & HEALTH SCIENCES
TERESA HAWLEYTERESA HAWLEY
ALI RAMEZANIALI RAMEZANI
• NIH/NCINIH/NCI
RAZA ZAIDI: Laboratory of Cancer Biology & RAZA ZAIDI: Laboratory of Cancer Biology & GeneticsGenetics
GLENN MERLINO: Chief, Laboratory of Cancer GLENN MERLINO: Chief, Laboratory of Cancer Biology & GeneticsBiology & Genetics
PAUL MELTZER: Chief, Cancer Genetics BranchPAUL MELTZER: Chief, Cancer Genetics Branch
• Funding- Funding- NCI/NIH; Elaine Snyder Cancer Research NCI/NIH; Elaine Snyder Cancer Research Fund, GWUMCFund, GWUMC
E. De Fabo, EOH, SPHHS, GWUMCE. De Fabo, EOH, SPHHS, GWUMC
