3-19-2010

Brochure and Sponsorship Package.Lab UpdateRegional ConferenceContacts for further publicity. Dave Leake

from Surrounded by Science.Credit HoursSafety information?No meeting Next week – Happy Spring

Break!Movie Night April 1st

sRNA resubmissionMicC Mutagenesis

StandardizationAll target sequences contain an NheI siteAll target sequences contain one of the

following (EcoRI, SpeI, PstI, XbaI)Site directed mutagenesis

Each isocaudamer site must be removed before submission

E. Coli micC - ompCS. Typhimirium micC – ompC, ompD

6 MicC mutants5’ END3’ TerminatorRBS+67 siteRandom