CellReporter™

Image and quantify biologyThe solution for cell- and bead-based assays

www.moleculardevices.com/genetix GenetixNow part of Molecular Devices

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A multi-application imaging platform generating high quality, quantitative data

Intuitive, flexible workflows

The CellReporter™ system overcomes the challenges encountered when working with conventional cell- and bead-based assays. The system generates high quality, quantitative data by utilizing state-of-the-art imaging technology and image analysis software to image, analyze and report on individual cells or beads.

Run cell-based assays to quantify:apoptosis, cell cycle phases, cell differentiation, cell proliferation, cytotoxicity, lipid accumulation, mitotic index, protein translocation, reporter gene expression, cell viability ...

Image using white light and fluorescence detection (<8 min for 2-channels in a 384-well plate).

Set-up assay in standard 96- or 384-well microplates.

Differentiate between small objectsLocate and quantify fluorescence.

fluorescence fluorescence

Fast image acquisition at sub-cellular resolution (0.8 µm)

Accurate identification of each cell or bead

Raw image of adherent CHO cells stained with propidium iodide-Texas Red filter set

Nuclei of dead cells identified using propidium iodide

Image Analyze

To increase throughput, the system can be readily integrated into robotic systems.

Run bead-based assays to quantify protein production during:hybridoma screening, antibody production,hormone secretion, cytokine production, growthfactor production ...

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Analyze populations based on morphological or fluorescent statistics for each cell or bead.

Visualize data as scatter plots, histograms, images, pie charts, heat maps.

Display relative proportions of identified sub-groups per well.

Normalize data to cell or bead number.

Generate customizable reports for export.

Live CellsLive Cells

Dead CellsDead Cells

Incr

easi

ng D

MS

O c

once

ntra

tion

Generation of statistics and QC of data

Results review Report and export

Cell viability per well Dose response curve

Report

Visit www.genetix.com to hear Dr. Tobin Dickerson from The Scripps Institute present

“Phenotypic screening using CellReporter to identify cures for the deadliest poison”Dr. Dickerson discusses the development of a whole cell based assay for Botulinum neurotoxin (BoNT)

inhibitors using the CellReporter system. By multiplexing a functional assay with other drug development filters,

such as cytotoxicity, lead compounds are efficiently triaged to facilitate optimization of compounds that enter into in vivo

studies of BoNT intoxication.

Info

Keep object

Exclude undesired objects from analysis

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Quantify cellular responses cell-by-cell

Assessment of cell cycleImage acquisition and accurate object identification

Using cell-based assay formats, the CellReporter system enables determination of the biological response of each cell within a heterogeneous population.

Gate heterogeneous population into 3 sub-groups

Reveal cell cycle distribution per well

By revealing and quantifying changes in nuclear DNA content, CellReporter enables monitoring of cell cycle in heterogeneous populations.

• Use standard cell-based assay formats

• See condition of individual cells – view morphology

• Analyze populations based on statistics per cell

• Improve data quality – exclude debris

HeLa cells

fluorescence fluorescence

Distribution of total nuclear intensities in Texas Red channel

2N DNA

4N DNA

S phase

Diameter of pie chart = cell numberSlices in pie chart = apoptotic:non-apoptotic cells

2n DNA2n DNA

S phaseS phase

4n DNA4n DNA

Mitosis4N Condensed2N Condensed

Report

Image

Analyze

Images represented in histogram analysis

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white light fluorescence white light fluorescence

Untreated HeLa cells Treated HeLa cells

Reveal distribution of apoptotic cells per well

Gate non-apoptotic and apoptotic populations

Images represented in histogram analysis

Image capture using white light and fluorescence

Assessment of apoptosis in a heterogeneous populationAccurate identification of cell nuclei

By determining the presence of condensed and fragmented chromatin, CellReporter enables quantification of the morphological change that occurs when cells undergo apoptosis.

Untreated (non-apoptotic) HeLa cells

fluorescence fluorescence

Treated (apoptotic) HeLa cells showing condensed, fragmented chromatin

Apoptotic

Non-apoptotic

Distribution of standard deviations of nuclear intensities in DAPI channel.

Untreated (non-apoptotic) HeLa cells

Treated (apoptotic) HeLa cells

Diameter of pie chart = cell numberSlices in pie chart = apoptotic:non-apoptotic cells

Non-ApoptoticNon-Apoptotic

ApoptoticApoptotic

Visit www.genetix.com to view more applications and download detailed technical notes

Report

Image

Analyze

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Quantify production of secreted proteinsTypically used to quantify the production of secreted proteins, bead-based assays performed using CellReporter are based on a simple, homogeneous assay format.

Reveal fluorescent bead intensity per well Generate standard curve from exported data

• One incubation step, no washing

• Analyze populations based on statistics per bead

• Improve data quality – exclude fluorescent debris and bead aggregates

Quantification of IgGImage acquisition and accurate bead identification

Beads Beads identified Beads bound to human IgG

white light white light fluorescence

Visit www.genetix.com to view more applications and download detailed technical notes

Plate Overview – heat map

Ass

ay R

eado

ut S

igna

l (a.

u.)

IgG Concentration (ng/ml)

Report

Image

Analyze

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Excellent imaging, intelligent analysis

System Specifications

Imaging

Software

Light source

White light imaging

Fluorescence imaging

Data tracking

Camera

Imaging speed

Resolution

Instrumentation

Source plate type

Source plate capacity

Instrument dimensions

Instrument Weight

Integration

Regulatory approval

CellReporter

Dedicated imaging software pre-installed on a high specification PC, Microsoft Windows XP (Professional SP3)

Xenon arc lamp

Trans-illumination

Software-controlled switching between up to 6 excitation/emission filter pairs

1 x internal barcode reader for data tracking for each plate

Integrated 16-bit cooled EMCCD camera

384 well microplate: 3 min per channel

Standard: 0.8 micron

96-, 384-well microplates

1 x plates

720 mm (width) x 458 mm (height) x 570 mm (depth)

55 kg

OEM integration kit available

CE marked

www.moleculardevices.com/genetix

ClonePix, CloneSelect, CellReporter are trademarks of Molecular Devices (New Milton) Ltd. All third party trademarks are the property of their respective owners.

For a listing of trademark owners, visit www.moleculardevices.com/genetix

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Unrivalled solutions based on excellent imaging and intelligent image analysis

Products from Molecular Devices offer scientists unrivalled solutions that utilize imaging and intelligent image analysis to support basic research, pharma-ceutical and biotherapeutic development. The company’s systems continue to establish industry standards in areas such as picking microbial colonies for genomic studies or screening and selection of mammalian cell lines. Other systems use imaging platforms to monitor cell growth, evaluate cellular responses and quantify

protein production. Through its expertise in robotics, cell and molecular biology, image analysis and interpretation, supported by a strong IP portfolio, the company is committed to the continual development of innovative solutions for life science applications.

For more information, visit www.moleculardevices.com/genetix