Download your free 30-day trial of ZEN Intellesis for

powerful image segmentation in 2D and 3D.

www.zeiss.com/zen-intellesis

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SCRATCH AREA

Based on the segmentation results, parameters can easily be analyzed with the ZEN Image Analysis Module.

Easily classify image data such as scratch area or mitotic cells with ZEN Intellesis.

Standard wound healing assay with GFP expressing HeLa cells.

ZEISS ZEN IntellesisImage Segmentation for 2D and 3D Datasets

Easily Segment Your Images by

Machine Learning

Segmentation is one of the biggest chal-

lenges faced by today’s microscopists. To

acquire images is the first step. Generating

data often requires more: an image pro-

cessing specialist, who can create a work-

flow for segmentation using a combination

of digital filters and tools. With image seg-

mentation using machine learning you can

avoid errors and influences of user bias.

ZEN Intellesis is your software module for

powerful machine learning segmentation

of multidimensional images including 3D

datasets. You can smoothly integrate mul-

tiple imaging modalities or achieve superior

segmentation on any single image. Use

your expertise to train the software and let

ZEN Intellesis do the tedious segmentation.

Highlights

• Segment your images to create

superior and precise results.

• Efficiently work with images from

different microscope modalities. Train

the software and seamlessly integrate

your segmentation in your ZEN image

analysis workflow.

• You only need to know your structures

of interest. Images are simply loaded,

classes are defined, objects are labelled

and the model is trained. When satis-

fied, you can use your trained software

to segment and analyze full datasets.

• ZEN Intellesis can be used with image

formats readable by the ZEN imaging

software including CZI, TXM, TIFF, JPG,

PNG and more.

Research Areas

• Neuroscience

• Cell Biology

• Cancer Research

• Developmental Biology

• Plant Biology and many more

Segment Images from Diverse

Imaging Modalities:

• Widefield Microscopy

• Superresolution Microscopy

• Fluorescence Microscopy

• Label Free Microscopy

• Confocal Microscopy

• Light Sheet Microscopy

• Electron Microscopy

• X-ray Microscopy

• Any other imaging technique

Download your free 30-day trial of ZEN Intellesis for

powerful image segmentation in 2D and 3D.

www.zeiss.com/zen-intellesis

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ZEISS ZEN Intellesis Advanced Image Processing across Multiple Microscopy Methods

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EMBRYO SIZE (mm²)

Created for Your Applications

• Scratch Assays

• Growth Assays

• Multifluorescence Imaging

• Neurite Growth

• 3D Datasets

• Cell Type Classification

• Visualization of Cell Compartments

• Segmentation of Spines vs. Dendrites

• Tissue Detection

• And many more

1. Mouse Embryo growth assay, Celldiscoverer 7 (brightfield); Data produced with ZEN Image Analysis; Courtesy of Max Planck Institute, Berlin

2. GFP labeled neuron – dendrite and spines classi-fied; Elyra structured illiumination

3. Drosophila Calyx region, classification of presynap-tic vesicles and other cell compartments; electron microscope, Courtesy of Max Planck Institute, Berlin

4. Fluorescently labeled muscle cells, myosin variants and cell membranes classified; LSM 880 with Airyscan FAST; Courtesy of Dr. Jia Li