Images from Angiography 2.0

Angiography 2.0 2

PCA & Camera

Angiography 2.0 3

Image quality innormal rabbits

Angiography 2.0 4

Looks can be deceiving(Pages 5-10)

-Fluorescein angiography shows less florid leakage 2 days after intravitreal injection of VEGF in rabbit compared to one week after injection (page 5). Similar results can be shown by fluorescein channel (Page 6). -40KDa channel (page 8) shows leakage at day 2 and no leakage at 1 week. This indicates that at day 2 post VEGF injection, the BRB is more permeable to 40 KDa particles than at one week. Which suggests a more severe BRB dysfunction-This in turn suggests the presence of a discrepancy between the qualitative assessment of fluorescein angiography leakage (based on area of distribution and intensity of color) and permeability to macromolecules.

-FA leakage grading may not be directly correlated to BRB status. -PCA may provide new information about BRB dysfunction not provided by FA

Angiography 2.0 5

Fluorescein Ameri et al 2007

Angiography 2.0 6

Day 0

Day 2 Day 8

400 Da

Angiography 2.0 7

Day 0

Day 2 Day 8

10 KDa

Angiography 2.0 8

Day 0

Day 2 Day 8

40 KDa

Angiography 2.0 9

Day 0

Day 2 Day 8

70 KDa

Angiography 2.0 10

Angiography 2.0 11

“to some journey… to some a destination…”

-PCA shows different patterns of flows for different components. -Smaller particles (400 Da and 10 KDa) go through an arterial phase, neovascular membrane (NVM phase) followed by leakage and with no venous phase. (pages 12, 13)-40 Kda particles (page 14) travel from artery followed by a venous & NVM (almost simultaneously) phase and no leakage.-70 Kda particles (page 15) show an arterial phase followed by a venous phase and delayed staining of the NVM.

Angiography 2.0 12

First Detection/Arterial Phase Venous/NVM

Phase

1-1.5 minutes 3-4 minutes

400 Da

Angiography 2.0 13

First Detection/Arterial Phase

Venous/NVM Phase

1-1.5 minutes 3-4 minutes

10 KDa

Angiography 2.0 14

First Detection/Arterial Phase

Venous/NVM Phase

1-1.5 minutes 3-4 minutes

40 KDa

Angiography 2.0 15

First Detection/Arterial Phase

Venous/NVM Phase

1-1.5 minutes 3-4 minutes

70 KDa

Angiography 2.0 16

Angiography 2.0 17

New technology

New perspective

New findings

Angiography 2.0 18

New patternsOf Auto-fluorescence

19Angiography 2.0

Enhanced nerve fiber layer visibility

Angiography 2.0 20

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