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Impact of altered cholinergic tones on the neurovascular coupling ...

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Accepted manuscripts are peer-reviewed but have not been through the copyediting, formatting, or proofreading process. Copyright © 2017 the authors This Accepted Manuscript has not been copyedited and formatted. The final version may differ from this version. Research Articles: Systems/Circuits Impact of altered cholinergic tones on the neurovascular coupling response to whisker stimulation Clotilde Lecrux 1,2 , Claire H. Sandoe 1 , Sujaya Neupane 2 , Pascal Kropf 2 , Xavier Toussay 1 , Xin-Kang Tong 1 , María Lacalle-Aurioles 1 , Amir Shmuel 2 and Edith Hamel 1 1 Laboratory of Cerebrovascular Research 2 Laboratory of Brain Imaging Signals, Montreal Neurological Institute, McGill University, Montréal, QC, Canada DOI: 10.1523/JNEUROSCI.1784-16.2016 Received: 25 May 2016 Revised: 22 December 2016 Accepted: 30 December 2016 Published: 9 January 2017 Author contributions: C.L., C.S., A.S., and E.H. designed research; C.L., C.S., P.K., X. Toussay, and X. Tong performed research; C.L., C.S., S.N., P.K., X. Toussay, X. Tong, and M.L.-A. analyzed data; C.L., C.S., S.N., M.L.-A., A.S., and E.H. wrote the paper; S.N. and M.L.-A. contributed unpublished reagents/analytic tools. Conflict of Interest: None declared. Funding: Supported by grants from the Canadian Institute of Health Research (CIHR, MOP-84209 and MOP-142417, EH; MOP-102599, AS), the Heart & Stroke Foundation of Canada (EH), the Human Frontier Science Program (RGY0080/2008, AS), a Jeanne Timmins Costello Postdoctoral Fellowship (CL), and a Jeanne Timmins Costello studentship (CHS). Corresponding author: Dr. Clotilde Lecrux, Laboratory of Cerebrovascular Research, Montreal Neurological Institute, 3801 University Street, Montréal, QC, Canada H3A 2B4, E-mail: [email protected], Tel: (+1) 514 398 8931 Cite as: J. Neurosci 2017; 10.1523/JNEUROSCI.1784-16.2016 Alerts: Sign up at www.jneurosci.org/cgi/alerts to receive customized email alerts when the fully formatted version of this article is published.
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Accepted manuscripts are peer-reviewed but have not been through the copyediting, formatting, or proofreadingprocess.

Copyright © 2017 the authors

This Accepted Manuscript has not been copyedited and formatted. The final version may differ from this version.

Research Articles: Systems/Circuits

Impact of altered cholinergic tones on the neurovascular coupling response towhisker stimulation

Clotilde Lecrux1,2, Claire H. Sandoe1, Sujaya Neupane2, Pascal Kropf2, Xavier Toussay1, Xin-Kang Tong1, María

Lacalle-Aurioles1, Amir Shmuel2 and Edith Hamel1

1Laboratory of Cerebrovascular Research2Laboratory of Brain Imaging Signals, Montreal Neurological Institute, McGill University, Montréal, QC, Canada

DOI: 10.1523/JNEUROSCI.1784-16.2016

Received: 25 May 2016

Revised: 22 December 2016

Accepted: 30 December 2016

Published: 9 January 2017

Author contributions: C.L., C.S., A.S., and E.H. designed research; C.L., C.S., P.K., X. Toussay, and X. Tongperformed research; C.L., C.S., S.N., P.K., X. Toussay, X. Tong, and M.L.-A. analyzed data; C.L., C.S., S.N.,M.L.-A., A.S., and E.H. wrote the paper; S.N. and M.L.-A. contributed unpublished reagents/analytic tools.

Conflict of Interest: None declared.

Funding: Supported by grants from the Canadian Institute of Health Research (CIHR, MOP-84209 andMOP-142417, EH; MOP-102599, AS), the Heart & Stroke Foundation of Canada (EH), the Human FrontierScience Program (RGY0080/2008, AS), a Jeanne Timmins Costello Postdoctoral Fellowship (CL), and a JeanneTimmins Costello studentship (CHS).

Corresponding author: Dr. Clotilde Lecrux, Laboratory of Cerebrovascular Research, Montreal NeurologicalInstitute, 3801 University Street, Montréal, QC, Canada H3A 2B4, E-mail: [email protected], Tel: (+1)514 398 8931

Cite as: J. Neurosci 2017; 10.1523/JNEUROSCI.1784-16.2016

Alerts: Sign up at www.jneurosci.org/cgi/alerts to receive customized email alerts when the fully formattedversion of this article is published.

in vivo

Animal preparation

Cortical blood flow measurement

Electrophysiology

Whisker stimulation

Identification of the neuronal network recruited by whisker stimulation

Enhanced ACh tone

Decreased ACh tone

Hemodynamic signals:

Electrophysiological data alignment:

Electrophysiological signals:

LFP/CBF correlations:

Cholinergic denervation:

C-Fos immunostaining in the barrel cortex:

Identification of c-Fos-immunopositive neurons in the activated barrel cortex:

Statistical Analysis:

r)

r z

R

vs

vs

vs

* *** r z

NK post-test

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