The Republic of the Union of Myanmar
Ministry of EducationBiotechnology and Material Science Research Department (BMSRD)
Biotechnology Research Department (BRD), Kyauk-se
Implementation of Nagoya Protocol on ABS
in
Biotechnology Research Department (BRD)
(Piloting of ABS Measures in MoU)
By
Dr.Nay Nwe Nyein Chan
Ph.D (Molecular Biotechnology)
Assisted Professor and Lab In Charge
Molecular Genetics Lab, BRD, Kyauk-se.
[email protected] , +95 092038063 9th, August, 2016
Outline
1. A Glance @ BRD
2. Current Status of Applying Biological Resources
3. Piloting of NP-ABS measures in BRD
4. Enhancement of MoU template for BRD as in piloting of
ABS measures
Gene Systems
A Glance
@
Biotechnology Research Department
• Built up as Department of Biotechnology, under the
Ministry of Science and Technology in RC2, Hlaing
Campus, Yangon Technological University (1996)
• Recruited as Training School of Special Biotechnology
since 2001 ( > 155 marks of Eng & Maths in matriculation)
• Moved to Mandalay Technological Unviersity in 2006
• Established as Biotechnology Research Department (BRD)
in Kyauk-se , 2015.
Historical Background
The Location of BRD
Ta-soe , Kyauk-se Tsp, Mandalay Region
12 acre for campus
23 acre for field research
BRD Constitution
Quantity
Faculty Staff 152
Staff 121
Total 273
Biotechnology Research Department
Director General
Deputy Director General
Molecular Biotechnology
Division
Environmental
Biotechnology Division
Aquaculture Biotechnology
Division
Food Biotechnology
Division
Microbiology and
Biotechnology Division
Administration and
Finance Department
Biotech Product and Process
Development Division
Animal Biotechnology
Division
Medical Biotechnology
Division
Plant and Agricultural
Biotechnology Division
Director
Director
Director
Director
Director
Director
Director
Director
Deputy Director
Director
Dy.Director
Dy.Director
Dy.Director
Dy.Director
Dy.Director
Dy.Director
Dy.Director
Dy.Director
Dy.Director
Senior Researcher
Senior Researcher
Senior Researcher
Senior Researcher
Senior Researcher
Senior Researcher
Senior Researcher
Senior Researcher
Senior Researcher
Junior Researcher
Junior Researcher
Junior Researcher
Junior Researcher
Junior Researcher
Junior Researcher
Junior Researcher
Junior Researcher
Junior Researcher
Director 1
Deputy Director 27
Assisted Director 84
Research Officer 54
Researchers 32
Lab Technician 31
Clerk/ Staff/ Drivers/ Lab Cleaners/
Security/Green House&Field Workers
63
Total 292
Faculty Members
1. Animal Biotechnology
cattle breeding, gastroenteritis, salmonellosis, probiotic food
2. Aquacultural Biotechnology
plankton, artemia, water quality control
3. Food Biotechnology
functional food, enzymes, bioyogurt, soymilk
SORGHUM SILAGE MAKING WITH LAB
Branches of Biotechnology (R&D)
4.Environmental Biotechnology
biodegradion, bioremediation, organic farming
5. Industrial Biotechnology
amino acids, antibiotics, chitin & chitosin, IAA,GA
6. Medical Biotechnology
antimicrobial, antifungal,anti-malarial, anti-cancer
7. Plant Biotechnology (PTC)
sugarcane, banana, mango, green tea, medicinal orchids,
carnation
Medicinal Orchid, Dendrobium officinale Myet Thit-kwa ( Dendrobium falconeri )
7. Plant Biotechnology (Mutation Breeding)
Local rice varieties, cotton, green gram
8. Molecular Biotechnology
plasmids and DNA extraction, molecular characterization,
DNA fingerprinting, protein analysis,
• Ph.D holders > 95
• Masters > 79
• Bachelors & Diploma > 66
• Undergrad Students > 52
• Regular Biotech Students can enroll in Government
Technological University (GTU) nowadays!
Human Resource Development (HRD)
• Field wide seminar (every Tuesday)
• Knowledge Sharing Seminar (ocassionally)
• Heads of Departments (HOD) meeting (every Tuesday)
Extra-curriculum Activities of BRD
• Abidhamma Class (every Wednesday & Friday)
• Offering meals to monks & novices (every Friday)
• Offering of Waso Robes & Food (every Sabbath days)
• Blood Donation Event (Four Times/Year)
• Vaccination (for researchers, staffs and their families)
Vision
to create a large pool of highly skilled personnel in different areas of biotechnology
To develop biotechnological processes, designs, techniques, equipments and solutions which can provide applications in the field of agriculture, food, medicines/ pharmaceutical , industry, health care services and environmental sciences
Mission
To launch several important programmes relevant to national needs and efforts with significant investment using biotechnological tools for generation of products, processes and technologies to enhance the efficiency and productivity of our country
Laboratories at BRD
1. Microbiology lab.
2. Mycology lab.
3. Pharmaceutical research lab.
4. Plant tissue culture lab.
5. Environmental lab.
6. Cell culture lab.
7. Food research lab.
8. Molecular genetics lab
9. Insectary lab.
10. Qualities analysis lab.
11. Wet lab and Aquaculture lab
15
2. Current Status of Applying Biological Resources
AYAminn mutant
Control
Mutant
Photoperiod insensitive
(PBK)Manawthukha
1. Yield & Quality Improvement of Myanmar local Rice Varieties through Gamma
Induced Mutation (IAEA projects)
flooding resistanceDrought tolerance
IAEA/RAS- 5045, 5065, 5056 and IAEA TC-5020 Projects
Salt tolerance
2. Integrated Plant Mutation Breeding with Best Fit Soil and Water Management
Practices for Climate Change.
Radio isotope labelling 15 N
8/11/2016 17
Radio isotope labelling 15 N
Established in 2012Supported by IAEA
Rice Quality Evaluation Lab
Parameters
• Milling Process(remove hull, whitener, grain grader)
• Grains >>
Eating quality (aroma, softness, stickiness, whiteness, polishing, taste)
Gelatinization temperature (GT)
Whiteness
Chalkiness
Grain shape (length and width ratio)
• Powder>>
Gel consistency (GC)
Amylose content (AC)
Measure grain length and width
AC Standards of potato starch and 5 china rice
GC TestAC Test
Eating Test
GT Test
Rehulling Polishing Grading Whitness
Chalky Test
3. IAEA/ RCA 5070 White seed mutants SSM-1 of Shwe ni-15 in M3 generation
20
4. Horticulture through plant tissue culture
ပိန ်းပန ်း
လလီီ
Aloe vera Artemisia Anthurium
Chrysanthemum
Lilium sp. pineapple Vitis spp.Desmodium Aritolochia21
Dendrobium
sp.
Vanda sp.Oncidium
sp.
> cattle breeding by artificial insemination (AI)
> Breeding of Myanmar cow and Friesian
> Rearing and conservation of Mithun (Bos frontalis)
SORGHUM SILAGE MAKING WITH LAB
5.Myanmar cow upgrading project
6. Mycology research
Biopesticide
BLB
Aspergillus
Leaf litter fungi
7. Pharmaceutical research based on Myanmar medicinal plants
Anti-malarial research
Anti-cancer research
Anti-diabetic research
Secondary metabolites research for antimicrobial activity
Antioxidant activity screening25
8. Cell Culture Research (Morphology of RD cell line)
Anti Cancer and Anti Diabetes
Sterile
Sterile
Ionization Radiation
Wild
No offspring
Birth Control Method
Mass rearing Pupae Release
9. Dengue control through SIT (IAEA/RCA 5066)
Fruit juice production (Noni, guava, mango, etc.)
Flavor enhancer preparation, Fermented fish
Kumbucha; fermented tea juice, Biological Soy-sauce preparation
Probiotic yoghurt production
Fig. Brine salt fermentation for soysauce
Fig. The process for fermented noni juice as neutraceutical
10. Food Biotechnology Research
11. Aquaculture research : SCP
Yeast culture Microscopic morphologyု ု Broth cultureBroth culture
FiltrationDrying
Survellance on fish diversity in Swan Ye Inn
12. Bacterial hormone research
Bacterial culture and their
microscopic morphology
Screening on bacterial hormone
The effect of bacterial hormone on sprouting and rooting
Product formulation
Waste water treatment and deodorant
Bioremediation of polluted soil
31
13. Environmental research
DNA Sequencing for identification of indigenous bacteria
Molecular Identification of endophytic bacteria in Herbal Plants
Transformation and Gene Cloning for antioxidant enhancement in freshly fruits
14. Molecular Genetics Researches by DNA sequencing and Molecular Marker
Techniques
(1) MBS Medium
DNA Sequencer (Genetic Analyzer 3130x)
Thermal Cyclers (PCR Machines)
Endophytes
from Thanakha
Marker Assisted Screening of mutant rice varieties (SSR
Profilling)
Phylogenic Studies for local Rice Varieties RM 232 RM 17
Shimuri
IR5
Nbare
Amaroo
T.Yar
Dongara
S.T.Yin
MR-9
Local.V
K.Nhyin
L.T.Hmwe
K.Yway
S.Y.Aye
K.N.Kyant
S.T.Yin*
K.N.KyantH
K.N.Kham
S.N.Yin
K.M.Koot
0.000.050.100.150.200.25
Herbal Medicines
Anti-aging Pills
Hair Nutrient Pills
Healthy Heart Pills
Anti-inflamatory Balm
Weight Loss Pills
Super Antioxidant Activity
Carotene supplement for heart
& prostate cancer patients
Lycopene supplement for
lung cancer patientsTomato Scented Candle
Present status of Myanma tomato market
Biotechnological Approach to make Tomato as
commercially important fruit in industrially high demand
Fresh, organic, cheap (50-500ks per viss)
Myanma Mangoes
• There are over 300 varieties of the fruit in Myanmar.
• The Sein Ta Lone is famous as the best mango in Myanmar
because of its delightful aroma, sweetness, juiciness and non-
fibrous nature.
• The Sein Ta Lone is growing prominently in central and tropical
region of Myanmar(especially in Kyaukse).
• Also, the Yin Kwe (Broken Heart) Shwe Hintha (golden
brahmin duck) are unique to Myanmar and of prime export.
quality.
Why Mango ?
• As genetic erosion of landraces diversity is increasing, evaluation
has become a necessity for on farm and ex situ conservation, at least
for most important types.
• As market expands to international level and easy & free trade are
leading to the bio-prospection, it’s considered to control genetic
introgression to indigenous Mangoes.
• Sein Ta Lone becomes famous and attractive to the global economy.
It is critical to prevent from bio-piracy.
KING of Fruits
Cuisine
Salad
Pickle
Jelly
Icecream
Jam
Sheet
Candy
Juice
Healthy Fruit
Objectives
• To study the genetic distinctiveness and relatedness among
Myanmar indigenous mangoes by molecular techniques
• To prevent indigenous varieties from biopiracy
• To conserve genetic resources in Myanmar
Proposed Strategy Sample Collection
Morphological Characterization Methods
Genomic DNA Extraction
DNA fingerprinting by PCR (URP, SSR, ISSR)
Gel Electrophoresis by Agarose & Polyacrylamide
Data Analysis by bioinformatics softwares (NTSYS , AMOVA, etc)
Service for DNA, PCR and RT-PCR
• For Both Bacteria & Plants
• For Aquatic Animals
Current Charges >> 10000ks per sample Fig., 1.4% Agarose gel electrophoresis of 16s
rDNA (800 bp) PCR products for Xanthomonas spp
Gradient PCR machines
Cell Lysis in
extraction buffer
Explants Gel Electrophoresis
Gel Imaging System
DNA check by Nanophotometer
Real-Time PCR
Step-One Plus
Research activities at BRD
I. R and D products
Biofertilizer production
Plant growth stimulator
New rice variety released
Banana plantlet, Anthurium white, Aloe vera
II. On going research
Mutation Induction of some Myanmar rice varieties via r ray (IAEA projects)
Mutant characterization by Marker Assisted Selection technique
Horticulture through plant tissue culture
Myanmar cow upgrading project
Microbial hormone and mycology research
Myanmar medicinal plant research and dengue control through SIT
Food research
Aquaculture research; SCP for aquatic feed
Environmental research
R and D products
Powder formPellet formPellet
form
1. Biofertilizer products
Shwe thee pwint
Blooming and fruit
N2 fixer
P solubilizer
K decomposer
46
Shweziwa biofertilizer
2. Chitosan based liquid foliar
Deproteinization
(Shrimp shells are treated with 4NaOH% at room temperature for overnight)
Demineralization
( by stirring with 20% HClfor 20 minutes, chitin are obtained.)
Deacetylation
(Chitosan can get by treating with 40% NaOHon 8000Watt stove for 6 hours
Shwe sein47
Shwe Poe Hnin, Shwe Seyay
3. New variety released :
Rice var. Sin Shwe Se from Sin thwe latt IR 53936 Philippines
Control
Early
maturity
Mutant
Date released: 30.6.2015
Trait : early maturity
Source: Gamma radiation
48
SSR Profile for True Mutant Type
Certification by National Seed Committee
Shwe ni
Khun wah Phee Gyann
Multiplication stage Hardening stage
49Anthurium sp. Aloe vera
Myanmar-Japan Joint research on cellulolytic nitrogen fixing bacteria ( Shibaura University)
Post Doctoral Research in India granted by TWAS/CSIR Fellowship
Short Term Training and Master courses on BIOTEC in India and Thailand supported by ASEAN foundation and NCGEB
AUN/SEED-NET (phase II) professor dispatch program, institute of genetic resources, plant genetic resources, faculty of agriculture, Kyushu University, Japan.
AUN/SEED-NET program for Master courses in Philippines
IAEA Technical Cooperation Expert Mission to DBT( MYA/0/007-12) on Transfer of molecular marker techniques
Application of radiation and radioisotope in agricultural biotechnology (MYA/501401)
IAEA TC project on mutation breeding and selection (MYA/045)
International Co-operation
• IAEA Technical cooperation project MYA/5/014 “Support for a feasibility study on using sterile insect technique against DBM, Plutella xylostella”
• IAEA TCP MYA/5/017 “ Studying yield improvement of local rice varieties through induced mutation” and Phase 2 (MYA/5/020)
• IAEA TC RAS/5/066 “Promoting the sharing of expertise and infrastructure for dengue vector surveillance towards integration of the sterile insect technique with conventional control methods among South and South East Asian Countries”
• IAEA project RAS/5/5052 “ Sharing regional knowledge on the use of the sterile insect technique within integrated area-wide fruit fly pest management programs”
• IAEA RAS /5/045 “ Improvement crop quality and stress tolerance for sustainable crop production using mutation techniques and biotechnology”
• IAEA RAS/5/056 and IAEA RAS/5/065 “ Development of climate change adaptable varieties var. Manawthukha and Ayamin”
• IAEA RAS/5/070 “ Developing bioenergy crops to optimize marginal land productivity through breeding and related techniques”
51
IAEA Collaboration Projects
How do they apply Genetics Resources ?? and How Many?
Microbial Genetics Resources
• Microbiology Lab
• Mycology Lab
• Environmental Lab
• Food Lab
• Aquaculture & Wet Lab
• Culture Collection Unit
• Molecular Genetics Lab
Plant Genetics Resources
• Plant Tissue Culture Lab
• Cell Culture Lab
• Pharmaceutical Research Lab
• Cotton Section
• Field Lab
• Quality Evaluation Lab
• Molecular Genetics Lab
~ 40 millions of Bacteria in 1g of soil
~ 1 million of Bacterial Cells in 1 ml of Fresh Water
3. Piloting of NP-ABS measures in BRD
(i) Process Meetings and Discussions
• HOD meeting (30th, May,2016)
• Knowledge Sharing Seminar (31st, May, 2016)
• Workshop Minutes Report to the Ministry (2nd, June, 2016)
• HOD Discussion for draft MoU template to be enhanced (21st,June,2016)
• Implementation of NP-ABS in enhancement of MoU (13th, July, 2016)
• Seminar on piloting of ABS measures in new MoU (20th, July, 2016)
• Submission of enhanced MoU and Official Permission (20th, July, 2016)
Dr. Aye Myint (Director General, BMSRD)
Dr. Aye Aye Khai (Director, BRD)
Dr. San San Yu (Deputy Director, Microbiology Lab)
Dr. Khin Mar Mya (Deputy Director, Cell Culture Lab)
Dr. Aye Aye Win (Deputy Director, Food Lab)
Dr. Myat Min (Deputy Director, Field Lab)
Dr. Win Min Than (Deputy Director, Mycology Lab)
Dr. Khin Cho Thein (Assisted Director, Shwe Sein Lab)
Dr. Ko Ko Win (Deputy Director, IR Section)
Dr. Kay Thi Oo (Deputy Director, PTC Lab)
Dr. Htet Htet Win (Assisted Director, PRD Lab)
Dr. Nandar Aye Win (Deputy Director, Aqua Lab)
Dr. Yin Zar Chi Htwe (Assisted Director, Culture Collection Lab)
Dr. Htwe Htwe Aye (Assisted Director, Breeding Unit of Myanmar Cow)
Dr. Nay Nwe Nyein Chan (Deputy Director, Molecular Genetics Lab)
Dr. Aung Kyaw Swe (Assisted Director, Shwe Zewa Production)
(iii) Duration of piloting: 13th, June, 2016 to 20th , July, 2016
(ii) People involved in the Enhancement of MoU Template
( Heads of Departments in BRD and Director General of BMSRD)
(iv) Name of collaborators in using the enhanced template/ framework agreement
>BRD, Myanmar and YAAS (Yunnan Academy of Agricultural Sciences), China
(v) Nature of collaboration:material, and specific intent of use on material/objectives;
>Agricultural Research Collaboration
(vi) Items discussed between collaborators on the items in the enhanced template/form;
>Articles : 1, 3, 4, 5, 6, 7 mainly
(vii) Result of discussions or agreements between collaorators on finalizing the enhanced
template/form;
>Both parties signed the framework agreement and also agreed to sign the
MoU after recieving government permission
(viii) Activity photos
During Piloting of ABS
HOD Meeting
HOD Meeting
HOD Meeting
Knowledge Sharing Seminar
Framework Meeting with YAAS
Framework Signing with YAAS
(ix) Other information materials
References of MoU templates from
• Mandalay Technological University
• Yangon Technological University
• THE U.S. Environmental Protection Agency(EPA)
• Environmental Science Services Administration and Princeton University
• International Treaty (updated in 2013)
(http://www.planttreaty.org/content/texts-treaty-official-versions)
• Bureau of Land Management California Desert District
• The California Energy Commission Staff Concerning Joint Environmental
Review
Recommendations and Suggestions by
• ACB Regional Consultant, Ping Peria, ACB Program Specialist, Anthony
C.T.M, UNEP ROAP Program Officer, Makiko Yashiro
• ECD and BRD Faculty Members, Senior Officers from different Ministries
• IAEA Technical Officers
Articles in original MOU template
• Art1: Both uni as coordinators or direct contact among faculties
• mutual collaboration :
• a) joint research and others
• b) exchange of scientific information
• c)exchange of faculty members
• d) exchange of students
• e) access to various facilities of each institute
• Art2: themes, conditions for result utilization, specific visits, specific
exchanges, other cooperation will be negotiated for specific case (mutual
requirements)
• -negotiate financial arrangements at each time according to funding
• Art3: purchase health/casualty insurance or comparable insurace coverage
for staff/students exchange
• Art4: effect on the signing date, valid for 5yrs,validity may be extended
• Art5: before dissolution, provide not less than 6months notice
• Art6: if questions arises, resolve it by mutual understanding
• Art7: MOU in English
4. Enhancement of MoU template for BRD as in piloting of ABS measures
• 1. Article 1 (Purposes & Scope of Cooperation)
• 2. Article 2 (Composition and Contacts)
• 3. Article 3 (Negotiation and Funding)
• 4. Article 4 (Insurance, Biosafety & Biosecurity)
• 5. Article 5 (Access and Benefit Sharing in utilizing of genetic resources
And traditional knowledge)
• 6. Article 6 (Publications and Acknowledgement)
• 7. Article 7 (Participation of Third Party)
• 8. Article 8 (Implementation and Amendment)
• 9. Article 9 (Disagreements Resolution and Dissolution)
• 10. Article 10 (Signature)
Articles in New MoU template
• Article 6 Article 6. Access to Genetic Resources
• Article 8 Special Considerations
• Article 9 Contribution to Conservation and Sustainable Use
• Article 10 Global Multilateral Benefit-sharing Mechanism
• Article 12 Traditional Knowledge Associated with Genetic Resources
• Article 17 Monitoring the Utilization of Genetic Resources
• Article 23 Technology Transfer, Collaboration and Cooperation
Items and Conditions of NP-ABS Annex Used in this Pilot Project
to enhance MoU for BRD