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In-silico identification and classification of TIR domain from R gene family

in i eon ea enome C nus c n taxid: 3821

Supervisor :

Dr. Brahma Deo Singh,

Professor EmeritusSchool of Biotechnology, Faculty of Science

Banaras Hindu University, Varanasi

 

Ph.D. Student:

Vinay Kumar Singh,

Information officer, CBI, SBT, F.Sc. BHU, VNS-05

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1. Plants are attacked by a wide spectrum of pathogens, being the targets of viruses, bacteria, fungi, protozoa, nematodes and insects. Over the course of 

their evolution, plants have developed numerous defense mechanisms

including the chemical and physical barriers that are constitutive elements of 

plant cell responses locally and/or systemically.

2. Resistance genes (R-Genes) allowed the examination of their localization in

plant cells and the role they play in signal transduction during the plant

resistance response to biotic stress factors. R-Genes are genes in plant

enomes that conve lant disease resistance a ainst atho ens b

INTRODUCTION

producing R proteins.3. Once the R protein has detected the presence of a pathogen, the plant can

mount a defense against the pathogen. Because R genes confer resistance

against specific pathogens, it is possible to transfer an R gene from one plant

to another and make a plant resistant to a particular pathogen.

4. These R proteins also contain a nucleotide-binding (NB) domain and/or a

leucine-rich repeat (LRR) region. R protein activation often trigger a

hypersensitive response (HR). To prevent damage due to spontaneous

inappropriate activation.

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ROLE OF TIR DOMAIN

In plants, this domain occurs at the N terminus of a major subclass of cytoplasmic nucleotide-binding leucine-rich repeat (NB-LRR) family

resistance receptors (R proteins), which trigger defense responses

after perception of pathogen effectors (Chisholm et al., 2006; Jones

and Dangl, 2006; Rafiqi et al., 2009; Dodds and Rathjen, 2010).These responses include localized cell death known as the

hypersensitive response (HR). The LRR domain of plant R proteins

et al., 1999; Dodds et al., 2001; Shen et al., 2003; Rairdan andMoffett, 2006; Padmanabhan et al., 2009).

TIR domains in plants exist as a component of a family of multi-domain

proteins known as resistance (R) genes. Toll-like receptors (TLRs) are

a class of proteins that play a key role in the innate immune system.

The plant Toll/IL-1 receptor/plant disease resistance gene (TIR)

domains play an integral role in its immune system forming part of 

a defense mechanism against microbial infection.

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CAJANUS CAJAN GENOME

Taxonomy ID: 3821

Accession: PRJNA48381 ID: 48381Accession: PRJNA48383 ID: 48383

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RESULTS

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Structure of TIR Domain From Cajanus cajan PMDB ID: PM0078097

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Verify 3D

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SUPERIMPOSITION BETWEEN TARGET & TEMPLATE

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http://eds.bmc.uu.se/eds/australis.phpAuStrAlis

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Phylogenetic classification TIR Domain in Cajanus cajan