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In The Name Of God
Precipitation
Antigen/Antibody ReactionPrimary reaction:
Immunoassay :Like Elisa , RIA , IFA
Secondary reaction : Lattice formation like Agglutination , Hemagglutination Complement Fixation, Neutralization ,
Precipitation : soluble antibody reacts with soluble antigen
Tertiary reaction : Detected by affect on tissues or cells.
Secondary Phenomenon
precipitation: proteinsSeveral types:Protein precipitationSRID DID
ImmunoElectherophoresis :Counter current IEP Rocket IEP
SRID plate preparation: (Agarose �+ BUFFER (Ab (Anti IgG ) Punch SamplingIncubation in RT for 24hmeasuring of diameter making of curve Calculation of sample concentration
SRID:Single Radial Immuno diffusion or Mancini
SRID Plate
Single Radial Immuno diffusion :
In SRID :34 GAM = Is useful for measuring of IgG , IgA, IgM,C3, C4
Gel is mixed with Ab
Ag is in punch In Single Radial Diffusion: one component is fixed
(e.g. antigen-in this case it is IgG)
As the Ag diffuses into the gel it reacts with the Ab and when the equivalence point is reached a ring of precipitation is formed as
illustrated in Figure
The diameter of the ring is proportional to the concentration of Ag .
(Double Immuno Diffusion =)DID or
Ouchterlony ImmunodiffusionQualitative2 DiffusionApplication in research laboratoryIs not as a routin method now
Ouchterlony - Identity
Ouchterlony – Partial Identity
Ouchterlony Non-Identity
Turbidimetery
measuring the amount of light Passing Through the solution.
Nephelometery
Measures SCATTERED light
Immunoelectrophoresis
Counter current IEP : Qualitative Rocket IEP: Quantitative
Thanks For your Attention