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INSTRUCTIONMANUALSORVALL SUPERSPEED

ANGLE ROTORSGSA SE-12GS-3 SM-24SA-600 SS-34

Du Pont CompanyInstrument ProductsBiomedical DivisionNewtown, Connecticut 06470

DuPont Instruments

PN 28505Issued June 1977

Superspeed Angle Rotors DU POflt Instruments

This manual is a guide for the use of

SORVALL SUPERSPEED ANGLE ROTORS

Data herein has been verified and validated and isbelieved adequate for the intended use of theinstrument. If the instrument or procedures are usedfor purposes over and above the capabilities specifiedherein, confirmation of their validity and suitabilityshould be obtained, otherwise Du Pont does notguarantee results and assumes no obligation orliability. This publication is not a license to operateunder, or a recommendation to infringe upon, anyprocess patents.

Notes, cautions, and warnings within the text of this manual are used to emphasizeimportant and critical instructions.

WARNING: An operating procedure, practice, etc., which if not correctly followed, couldresult in personal injury.

CAUTION: An operating procedure, practice, etc., which, if not strictly observed, couldresult in damage of equipment.

NOTE: An operating procedure, condition, etc., which it is essential to highlight.

Health hazards precaution data. If and when hazardous chemicals or adverse healthaffect the environment or use of the equipment, appropriate precautions are provided.

OU Pont Instruments Superspeed Angle Rotors

TABLE OF CONTENTSParagraph Page

Section 1. DESCRIPTION

1-1 SCOPE 1-11-2 DESCRIPTION 1-11-3 TUBES, BOTTLES AND ADAPTERS 1-21-4 PARTS AND ACCESSORIES 1-91-5 RCF DETERMINATION 1-121-6 CRITICAL ANGULAR VELOCITY 1-18

Section 2. OPERATION

2-1 PRE-RUN CHECKS 2-12-2 TUBE, BOTTLE AND ADAPTER INSTALLATION AND ASSEMBLY . . . 2-12-3 ROTOR MOUNTING AND BALANCING 2-12-4 ROTOR "SPEEDVTEMPERATURE DIFFERENTIAL

DETERMINATION 2-32-5 REDUCING "SPEEDS" FOR DENSE FLUIDS 2-52-6 ESTIMATION OF SEDIMENTATION TIMES IN AQUEOUS

(NON—GRADIENT) SOLUTIONS 2-6

Section 3. MAINTENANCE

3-1 CORROSION 3-13-2 WASHING 3-13-3 CONTAMINATION 3-23-4 STORAGE 3-3

LIST OF ILLUSTRATIONS

Figure Page

1 -1 Sorvall Superspeed Angle Rotors vi1 -2 Glass Tubes and Bottles 1-41-3 Plastic Tubes and Bottles 1-41-4 Stainless Steel Tubes and Bottles for Superspeed

Angle Rotors 1.41-5 Adapters 1-51-6 Parts of the GSA and GS-3 Superspeed Angle Rotors 1-101 -7 Parts of the SA-600, SE-12, SM-24 and SS-34

Superspeed Angle Rotors 1-111 -8 Superspeed Angle Rotor Cross Section 1-172-1 Positioning Pairs of Tubes or Bottles in the Rotor 2-22-2 Sample Rotor "Speed'YTemperature Differential Chart 2-3

111

Superspeed Angle Rotors DU POflt Instruments

LIST OF TABLES

TablePage

1-1 Superspeed Angle Rotors: Basic Specifications 1-11-2 Key to Abbreviations Used in Tables 1-3, 1-4, 1-5 & 1-6 1-21 -3 Glass Tubes and Bottles for Superspeed Angle Rotor 1-31 -4 Plastic Tubes and Bottles for Superspeed Angle Rotors 1-51-5 Stainless Steel Tubes and Bottles for Superspeed Angle Rotors . . . . 1-71 -6 Adapters 1-71-7 Chemical Compatability of Rotor Elements 1-81 -8 Parts: Superspeed Angle Rotors 1-91-9 GSA Rotor: RCF and K Factor 1-131 -10 GS-3 Rotor: RCF and K Factor 1-131-11 SA-600 Rotor: RCF and K Factor 1-141-12 SE-1 2 Rotor: RCF and K Factor 1-151-13 SM-24 Rotor: RCF and K Factor 1-161-14 SS-34 Rotor: RCF and K Factor 1-171-15 Critical Angular Velocity of Superspeed Angle Rotors 1-132-1 Recommended Design Mass per Compartment

for Each Rotor 2-5

IV

OuPOHt Instruments Superspeed Angle Rotors

DU PONT INSTRUMENT SUPERSPEED ROTORWARRANTY

Every Sorvall Superspeed Rotor is warranted (subject to the conditions specified below andin the warranty clause of the Du Pont Instruments terms and conditions of sale in effect at thetime of sale) against defects in materials or workmanship for seven (7) years (properly reduced forcertain fluid densities, fluid gradients, tube assemblies, and adapters as described in theseoperating instructions).

Conditions

a. This warranty is valid for seven (7) years from the date of shipment to the original buyer byDu Pont Instruments or by any authorized Du Pont Instruments representative.

b. This warranty extends only to the original buyer and may not be assigned or extended to athird person without the written consent of Du Pont Instruments.

c. This warranty covers the rotor onlyand Du Pont Instrumentsshall not be liable fordamageto accessories or ancillary supplies including but not limited to (i) tubes, (ii) tube caps, (iii) tubeadapters, or (iv) tube contents.

d. This warranty is void if the rotor is (i) operated or maintained in a manner contrary to theinstructions in the manual for the rotor or centrifuge in use, or(ii) used in a Sorva 11 centrifuge thathas been modified without the written permission of Du Pont Instruments.

e. Should a Sorvall Centrifuge be damaged due to the failure of rotor covered by thiswarranty, Du Pont Instruments will supply, free of charge, (i) all centrifuge parts required forrepair and (ii) if the centrifuge is currently covered by a Du Pont Instruments warranty or serviceagreement; all labor necessary for repair of the centrifuge.

Superspeed Angle Rotors DuPont Instruments

6/7SA/2

Figure 1-1. Sorvall Superspeed Angle Rotors(Top, L-RJ GS-3, SA-600, GSA

fBottom, L-RJSM-24, SE-12, SS-34

VI

DuPont Instruments Superspeed Angle Rotors

Section 1. DESCRIPTION1-1. SCOPE.

This manual contains descriptive, operational and maintenance data for all Sorvall SuperspeedAngle Rotors, including the GSA, GS-3, SA-600, SE-12, SM-24 and SS-34 rotors.

1-2. DESCRIPTION.

Sorvall Superspeed Angle Rotors, are designed for use in the Sorvall RC-2, RC2-B, RC-5, andRC-5B Superspeed Refrigerated Centrifuges. With the exception of the GS-3, the rotors may also beused in the Sorvall SS-3 Automatic Superspeed Centrifuge. The rotors are machined from analuminum alloy forging for a high centrifugal force strength-to-weight ratio. Table 1 -1 provides basicspecifications for each superspeed angle rotor.

Table 1-1. Superspeed Angle Rotors: Basic Specifications

Diameter

Mass(weight)

Angle

Number ofPlaces

MaximumAngular Velocity(in rev/min)

MaximumRelativeCentrifugalForce (RCF)

GSA

31 cm(12-1/4 in)

14.9 kg(33 lbs)

28°

6

13 OOO/min

27 600

GS-3

33 cm(13-3/16 in)

21.4 kg(47 lbs)

20°

6

9 OOO/min

13 700

SA-600

27 cm(10-3/4 in)

10.2 kg(22.5 lbs)

34°

12

1 6 500/min

39 400

SE-12

19 cm(7-4/5 in)

4.1 kg(9 lbs)

40°

12

20 000/min

41 500

SM-24

24 cm(9-1/8)

7.7 kg(17 lbs)

28°

24

20 OOO/min

OuterRow 49 300

InnerRow 39 700

SS-34

23 cm(9 in)

6.8 kg(15 lbs)

34°

8

20 000/min

48 200

1-1


1-3. TUBES, BOTTLES AND ADAPTERS.

Each Sorvall Superspeed Angle Rotor accepts a variety of plastic, glass and stainless steel tubesand bottles. Special adapters permit the use of tubes and bottles other than those which fit thebasic rotor.

Tables 1 -3, 1 -4 and 1 -5 provide the following data necessary to select the appropriate tube orbottle of glass, plastic or stainless steel

a. For Use in Rotor (Adapter) — Lists the rotor or rotors which will accept that tube or bottle. If anadapter is required to permit the rotor to accept the desired tube or bottle, a key number will followtherotor initials in parentheses. Table 1 -6, Adapters, lists the adapters by key number and provides partnumbers and descriptions. Table 1-6 is keyed by part number to figure 1-5 which illustrates theadapters listed.

b. Part Number — Gives the part number assigned to each item by the Biomedical Division*.Tables 1-3, 1-4 and 1-5 are keyed by part number to figures 1-2, 1-3 and 1-4 respectively. The figuresillustrate the tubes, bottles and covers listed.

c. Nominal Capacity —Gives the rounded amount of milliliters of solution which each tube orbottle can contain.

d. Material — Gives an abbreviation for the specific glass or plastic of which the item is made.Table 1-2, Key to Abbreviations is provided for interpretation of the material code. Table 1-7,Chemical Compatability of Rotor Elements, is provided as an aid in selecting the tube or bottle bestsuited to the solution which will be used.

e. Nominal External Dimensions — Gives the rounded diameter and length of the tube or bottlein millimeters.

f. Covers — Gives material and part number of cover(s) if required with the desired tube or bottle.

Table 1-2. Key to Abbreviations Used in Tables 1-3, 1-4, 1-5 and 1-6

CABCGNNMORPPAPC

= Cellulose Acetate Butyrate= Corex®= Graduated= Nylon= Narrow Mouth= Oak Ridge Style= Pyrex= Polyallomer= Polycarbonate

PEPIPPRRBS/STTW

= Polyethylene= Plastic= Polypropylene= Rubber= Round Bottom= Stainless Steel= Tapered= Thin Wall

*The part number cannot be used to order replacement parts. Please refer to the Sorvall Tube, Bottleand Adapter Price List for the correct catalog number before ordering.

1-2


Table 1-3. Glass Tubes and Bottles for Superspeed Angle Rotors

For Use in Rotor (Adapter)

SA-600 (7); SE-12 (20);SM-24(17); SS-34 (7)

GSA(12 in 46); SA-600 (6);

SE-12(19); SM-24(16);SS-34 (6)

GSA(30); SE-12 (21);

SM-24(18)

SE-12 (22)

GSA(11 in 46); SA-600 (8);SM-24(15); SS-34 (8)

SA-600 (9); SS-34 (9)

SA-600 (9); SS-34 (9)

GSA(46)

SA-600 (5); SS-34 (5)

GSA (46)

SA-600 (5); SM-24(1)

SS-34 (5)

GSA (46)

SA-600 (2); SS-34 (2)

GSA (45); SA-600 (10);SS-34 (10)

SA-600, SS-34

GSA (13)

GSA (13)

GSA (14)

GSA (30)

GSA (30)

Tubes/Bottles

PartNumber

00120

00100

00124

00118

00101

00123

00125

00104

00119

00127

00152

00103

00128

00156

00105

00129

00116

00158

—

NominalCapacity

(ml)

1 (T)

3

5

5

10

10 (T)

10(T)(G)

12 <T)

12

14

15

20

30 (NM)

30

45

70

100

150

4

5

Material

P

P

P

P

P

P

P

P

P

P

C

P

P

c

p

p

p

c

Standardglass testtubes

Nominal ExternalDimensions(mm x mm)

8 x 63

10 x 75

12 x 75

14 x 60

13 x 100

18 x 102

18 x 102

17 x 120

178 x 102

18 x 120

18 x 102

18 x 120

28 x 90

24 x 105

29 x 101

44 x 128

44 x 137

53 x 132

10 x 75

12 x 75

Covers

Material

—

R

—

—

—

—

R

R

—

—

R

—

—

—

I PlasticI Cover S

—

PartNumber

—

00326

—

—

—

—

00321

00321

—

—

00304or

00331

—

—

—

Screwupplied

—

1-3


Glass Tubes/Bottles

V

00321

Q

00331

00321 X3

i

Q/Pi

00100 00101 00103 00104 00105 00116 00118 00119 00120 00123 00124 00125 00127 00128 00129 00152 00156 00158

Figure 1 -2. Glass Tubes and Bottles

Plastic Tubes/Bottles

U

icn3i\ AUU

00211 00242 00244 00246 00259 00276 00279 0028300241 00243 00245 00258 00268 00278 00282

00287 0028600701 00702 00702 00704 00706 00706 00704 00704

00701 00701 00702 00704 00704 00706 00706 00704

00700 00700 _ _ ZT1 ^ C . Co^ CS) (oi

00288 00291 00294 00721 00730 00732 00741 00770 00772 00781 00784 0099200289 00292 00720 00722 00731 00740 00742 00771 00780 00782 00991 00993

Figure 1 -3, Plastic Tubes and Bottles

Stainless Steel Tubes/Bottles00518

00522 00525

Figure 1 -4. Stainless Steel Tubes and Bottles

1-4


f Adapters

00370 00370

00380 00381 0038? 00388 00389 O0402 00408 00116 00419 00424 00425 00439 00449 00456 00458 00473

Figure 1-5. Adapters

Table 1-4. Plastic Tubes

For Use inRotor (Adapter)

SA-600 (33); SS-34 (33)

GSA (30); SA-600 (23)SS-34 (23)

GSA (30); SA-600 (23)SS-34 (23)

GSA (30); SA-600 (23)SS-34 (23)

SA-600 (50); SM-24 (34)SS-34 (50)

SA-600 (50); SM-24 (34)SS-34 (50)

SA-600 (50); SM-24 (34)SS-34 (50)

SA-600 (37); SS-34 (37)

SA-600 (37); SS-34 (37)

SA-600 (50); SM-24 (34)SS-34 (50)

GSA (31); SA-600 (32)SS-34 (32)

GSA (31); SA-600 (32);SS-34 (32)

GSA (31); SA-600 (32)SS-34 (32)

SE-12

and Bottles for Superspeed Angle Rotors

Tubes/Bottles

PartNumber

00211

00720

00721

00722

00730

00731

00732

00241

00242

00291

00740

00741

00742

00991

NominalCapacity

(ml)

1

4

4

4

7

7

7

10 (OR)

10 (OR)

1 0 (TW)

12

12

12

14

Material

CAB

PC

PP

PE

PC

PP

PE

PC

PP

PA

PC

PP

PE

PC


7x 50

10.6 x 75

10.6 x 75

10.6 x 75

1 3 x 100

13 x 100

1 3 x 100

16 x 80

16 x 80

1 3 x 100

1 6 x 100

1 6 x 100

1 6 x 100

18 x 75

Covers

Material

—

PP

PP

PP

PP

PP

PP

) Plastic Scr\ Cover Si

PP

PP

PP

PP

PP

PartNumber

00700

00700

00700

00701

00701

00701

ewjpplied

00227

00702

00702

00702

00704

1-5

Superspeed Angle Rotors Qll pgnt InStPlimeiitS

Table 1-4. Plastic Tubes and Bottles for Superspeed Angle Rotors (continued)


SE-12

SE-12

SA-600(32); SS-34 (32)

SA-600 (24); SM-24SS-34 (24)

SA-600 (24); SM-24;SS-34 (24)

SA-600 (24); SM-24SS-34 (24)

SA-600 (35); SS-34 (35)

SA-600 (35); SS-34 (35)

SA-600; SS-34

SA-600; SS-34

SA-600; SS-34

SA-600; SS-34

SA-600; SS-34

SA-600; SS-34

SA-600; SS-34

GSA (47)

GSA(47)

GSA

GSA (36)

GSA (36)

GSA (36)

GSA (38)

GSA (36)

GS-3

GS-3

PartNumber

00992

00993

00292

00770

00771

00772

00243

00244

00245

00246

00780

00781

00782

00784

00294

00288

00289

00258

00268**

00259

00276

00278

00279

00282

00283

Tubes/BottlesNominalCapacity

(ml)

14

14

1 5 (TW)

16

16

16

30 (OR)

30 (OR)

50 (OR)

50 (OR)

50

50

50

50

50 (TW)

150 (TW)

150

250 (NM)

250

250

250

250 (RB)

290

500

500

Material

PP

PE

PA

PC

PP

PE

PC

PP

PC

PP

PC

PP

PE

N

PA

PA

PC

PE

PE

PP

PC

PC

PC

PP

PC


18 x 75

18 x 75

1 6 x 1 00

1 8 x 100

1 8 x 100

1 8 x 100

25 x 90

25 x 90

29 x 104

29 x 104

29 x 102

29 x 102

29 x 102

29 x 102

28 x 104

45 x 122

45 x 122

60 x 137

61 x 122

61 x 122

61 x 122

61 x 136

61 x 138

70 x 1 66

70 x 166

Covers

Material

PP

PP

PP

PP

PP

PP

PlasticCover S

PP

PP

PP

PP

—

PP

PP

> Plastic' Cover

PI

PI

PI

PI

PI

PI

PI

PartNumber

00702

00702

00228

00704

00704

00704

ScrewSupplied

00706

00706

00706

00706

—

00287

00286

ScrewSupplied

00450*

00450*

00450*

00450*ring

00450*

00430*

00430*

*Plastic screw caps supplied but sealing caps 00450 or 00430 must be used for full volume, full speed operation.

** The 00268 bottles are limited to 8 000 rev/min.

1-6

DU Pont Instruments Superspeed Angle Rotors

Table 1-5. Stainless Steel Tubes and Bottles for Superspeed Angle Rotors


SM-24

SA-600; SS-34

SA-600; SS-34

GSA

GSA

GSA

Tubes/Bottles

PartNumber

00525

00517

00579

00521

00530

00522

NominalCapacity

(ml)

175

50

50

200

285

315

Material

S/S

s/s

S/S

s/s

s/s

s/s


18x96

28x 101*

2 8 x 1 0 1 * *

61 x 124

61 x 140

61 x 153

Covers

Material

—

S/S

S /S

—

PartNumber

—

00518***

00518***

—

Stainless SteelScrew Cover Supplied

S/S 00518***

*With flange"•Without flange^Requires wrench PN 01014

Item No.

1

2

b

6

7

o

9

10

11

12

13

14

15

16

17

18

19

20

Places

1

Pad

1

2

2

1

1

1

3

1

1

1

1

1

1

Material

R

R

R

R

R

R

R

R

PI

R

R

R

R

R

R

R

Table 1-6

PN

00324

00330

JC363

00364

00365

00366

00367

003C8

00^5?

00370

00371

00372

00373

00374

00375

00376

00377

00378

. Adapters

Item No.

21

22

23

24

30

31

32

33

34

35

36

37

i 38

45

46

47

50

Places

1

1

2

1

12

6

1

4

1

1

Pad

1

Pad

3

CJl

1

1

Material

R

R

PI

PI

PI

PI

PI

PI

PI

PI

PI

PI

PI

PI

R

PI

PI

PN

00379

00380

00381

00382

00388

00389

00402

00408

00416

00419

00424

00425

00439

00449

00456

00458

00473

1-7


Table 1-7. Chemical Compatability of Rotor Elements

Reagent

Acetaldehyde (100%)Acetic Acid (5%lAcetic Acid (60%)Acetic Acid (Glacial)AcetoneAllyl AlcoholAlum, ConcentratedAluminum ChlorideAluminum FluorideAmmonium AcetateAmmonium CarbonateAmmonium Hydroxide (10%) . .Ammonium Hydroxide (Cone.)Ammonium Persulfate (Sat'd.) .Ammonium SulfideAmyl AlcoholAnilineAqua FtegtaBenzeneBenzyl AlcoholBoric AcidBrineN-Butyl AlcoholCalcium ChlorideCalcium HypochlonteCarbon TetrachlondeCetyl AlcoholChlorine WaterChlorobenzeneChloroformChromic Acid I1O%IChromic Acid (50%lCitric Acid 110%)CresolCyclohexyl AlcoholDiacetoneDiazo SaltsDiethyl KetoneDimethylformamideDioxaneDistilled WaterEther DiethylEthyl AcetateEthyl Alcohol (50%)Ethyl Alcohol (95%)Ethylene DichlorideEthylene GlycolFerric ChlorideFluoboric AcidFormaldehyde (40%)Formic Acid (100%)Fuel OilGallic AcidGasoline (Refined)Gasoline (Sour)Hydrochloric Acid (10%) . . . .Hydrochloric Acid (60%) . . . .Hydrochloric Acid (Cone./ . . . .Hydrofluoric Acid (10%) . . . .Hydrofluoric Acid (100%) . . . .Hydrogen Peroxide (3%) . . . .Hydrogen Peroxide (100%). . . .HydroquinoneIsobutyl Alcohol

SSSSSSSMSSSSS

SSSSSSSSSsssss-sssssss-ss—ssssssssMss

ssssssuususs

-MuuMs

uM

___uusMuMM

ssssMMsu_MuuM

usM-MMMssMMMSsM__Us

ssuuuuusuMs

_sssMsss

_ssu

_s_MM-

s-ssss

susMM

s-

-sM--s—MSSMSSSMSS_s

ssususM_

s

usuuu

sss

_uuuusuMssssssss

ssssss

_u--M

usuuuusss

u_sssssssMussMs

suuuu

sss

usuuuMuuuus

ssuM_

uuuuus_-u--uusuussussuu

sMssuuuuuMuuM

sssss-

uM

s-__sssusss

sMMM_

u_-MusM

s

s

s-Mss

su

Ms

sssuuuuusss

ss_sssss-sss--s--sssssssss--s--sus--s

-s-ssssss

susss

s--s

s

ss

-ssss-suM

sssss-s-s

ss_suM—-ssuus-—--

-s--ss-—u

sussssuuuuuss

—

MsMMssssssssssssMuuusssssussuusssM

sssM

sMsM

sssusssssMsuusssssssss

usuuus-susuuu—us—uuusssMsu_suususu———uuusuussus„

sMs_sussMMuss_-

usuuuu—s_—suu

—u—usussus_susuMuus—uu—u—usuusuus—

_u

uusuuMuss_u

KEYS = Satisfactory, acceptable solutions for recommended

use.M = Mild attack, not recommended for use. If these

solutions must be used, avoid prolonged exposureand thoroughly clean and dry all rotor elements aftereach run.

U = Unsatisfactory, not recommended under any cir-cumstances.

— = Data unavailable at this time, not recommended foruse without prior testing.

Reagent

Isopropyl AlcoholLactic Acid (20%)Lactic Acid (100%)Lauryl AlcoholLead AcetateLinseed OilMaleic AcidManganese SaltsMagnesium HydroxideMercuryMethyl AlcoholMethyl Ethyl KetoneMethyl SalicylateMethylene ChlorideNickel SaltsNitric Acid (10%)Nitric Acid 150%)Nitric Acid 195%)Oleic AcidOxalic AcidPerchloric Acid (10%)PhenolPhenyl Ethyl AlcoholPhosphoric Acid I1O%IPhosphoric Acid (Cone.) . . .Phosphorus Trichloride . . . .Potassium AcetatePotassium CarbonatePotassium ChloratePotassium ChloridePotassium Hydroxide (5%) . . .Potassium Hydroxide (Cone.) .Potassium Permanganate . . . .Silicic AcidSilicone FluidsSilver Cyanide

Sodium BisulfateSodium BorateSodium CarbonateSodium Chloride 110%)Sodium Chloride (Sat'd.l . . .Sodium DichromateSodium Hydroxide (>1%) . . .Sodium Hydroxide (10%l . . .Sodium Hydroxide (Cone.). . .Sodium HypochlonteSodium Nitrate (10%)Sodium PeroxideSodium SulfideSodium ThiosulfateSulfuric Acid (10%)Sulfuric Acid 150%)SuKuric Acid 175%)Sulfuric Acid (Cone.)Tannic AcidTolueneTrichlorethyleneTrichloroethaneTrisodium PhosphateTurpentineUreaUrineXylenZinc Chloride

C

. s

. ss

• s. s. s. s. s. s. s. s. s. s. s. s. s. s. M. s

u. -

M. s. M. U. S. s. s

. s

. s

. M

. S

. —

. -

. s-

'. ss

. ssssM

ss-ssuuuussssssssss

s

sss-uM-MMss-uuus

uu

uuusM-

suuM-

suMMsss-uuuu—u—Muuuu—M-MMSssM—

sss--ss-sssM-

ussM

usssu

su

-ssssu-

—ssssss—ssuss—sssM

uussM

s—s——M

s

sss—uss-ssuu

MssssMsss

sssu

_suu

_s-sssss—MMus—sssssssss-s—s——ss

u————ss

M

ssuuusuuuuMu_uuuM

_

sMM__

s-sssss

MMMMMMsMUU

uusu-u—s-—us

_s

-Mss-—ussssMMMMSMUgs

—-_MsMUUs

-M—MSS-uuuMMSs—M-———

s

——ss—sM

f £

s-—s—--———ss———MMUssys

___ssss

s

_-ssssssssssssssMuuussss—sssus

ss—sss—sss——sssssssc

sss_ssssss

_ssssss—sssssss—uuuss—-—-————M

? ^

ssssssssssss

ssssussssssssssssssssMssssssssssssssssssusuuusMssus

F J? i

ss---s—-usuuuussMuss

u

ssuM_

ssuu„

ssss-—uuusu—ususss-uuuMussus

7/u—_uss-susuuuussMuss

usM—

ssssu

_

_ssss-

suus——s—suuu-s-s—ussss

NOTEThe critical components apt to come into contact with solutionwhen using a superspeed angle rotor are the rotor body (alumi-num) and the tube, bottle and/or adapter chosen for the run.Materials of tubes, bottles and or adapters may be obtained fromthe appropriate table 1-3, 1-4, 1-5 or 1-6.

IPDP-496B

1-8

Dll Pont Instruments Superspeed Angle Rotors

1-4. PARTS AND ACCESSORIES.

Parts for all Superspeed Angle Rotors are pictured in figures 1-6 and 1-7.

Both figures are keyed to Table 1-8, which includes all information necessary to:

• identify parts and accessories for assembly, operation and disassembly.

• order replacement parts or accessories.

To obtain replacement parts, contact any of the offices listed on the last page of this manual andinclude the part number and description of the desired item. To ensure that you receive the correctpart for your unit, be sure to include the rotor type and its serial and/or model number.

Table 1-8. Parts: Superspeed Angle Rotors

ItemNo.

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

Description

Rotor Body

Adapter-Sub Assy

Cap Screw

Seal Washer

Setscrew

Rotor Cover

O-Ring

Cover Sealing Stud

Flat Washer (1/4")

Lock Washer

Cover Stud TopWasher

Cover Seat Washer

Retaining Ring

Cover Seat

Cover Slamping Stud

O-Ring

O-Ring

O-Ring

Carrying Handle

Part Number

GSA

08140

08139

—

08132

—

08107

60356

08131

60751 (2)

28016

—

08125

60357

—

08120

61616

61617

60358

01047

GS-3

07010

07026

—

07004

—

07012

—

07024

60751 (2)

28016

—

08125

60357

—

07022

61616

—

—

10147

SA-600

28509

28021

63078 (3)

—

60574 (3)

28506

60810

28008

60751 (2)

28016

28010

28011

60067

28510

28006

61616

60189

—

01031

SE-12

27001

28021

63078 (4)

—

60574 (4)

27003

61720

28008

60751 (2)

28016

28010

28011

60067

28007

28006

61616

60189

—

01031

SM-24

29001

28021

63078 (4)

—

60574 (4)

29002

60696

28008

60751 (2)

28016

28010

2801 1

60067

28007

28006

61616

60189

—

01031

SS-34

28001

28021

63078 (4)

—

60574 (4)

28005

60188

28008

60751 (2)

28016

28010

28011

60067

28007

28006

61616

60189

—

—

1-9


6/7SA/7

SERIAL NO.

NOTEEarlier Models may not conform exactly toillustration.

1-10

Figure 1-6. Parts of the GSA and GS-3 Superspeed Angle Rotors


15

7 6

SERIAL NO.

Figure 1-7. Parts of the SA-600, SE-12, SM-24 and SS-34Superspeed Angle Rotors

1-11


1-5. RCF DETERMINATION.

Relative centrifugal force (RCF) is determined by the "common usage" formula:

when r = the radius in centimeterscj = the angular speed in revolutions per minute

Figure 1 -8 shows the minimum, average and maximum radii of a superspeed rotor. The value foreach radius may be found in the appropriate table 1 -9 through 1 -14. With this information, RCF forthe rotor at a given speed can either be calculated by the formula given above or extracted from theappropriate table.

Figure 1-8. Superspeed Angle Rotor Cross Section

1-12


Table 1-9. GSA Rotor: RCF and K Factor

AngularVelocity(rev/min)

1 0001 5002 0002 5003 0003 5004 0004 5005 000

5 5006 0006 5007 0007 5008 0008 5009 0009 50010 00010 500

11 00011 50012 00012 50013 000

RCF (Radius)r minimum9.27 cm(3.65 in)

104233414647932

1 2701 6602 1002 590

3 1303 7304 3805 0805 8306 6307 4808 3909 35010 40011 400

12 50013 70014 90016 20017 600

1 average11.94 cm(4.70 in)

133300534834

1 2001 6302 1302 7003 330

4 0304 8005 6406 5407 5008 5409 640

10 80012 00013 30014 70016 10017 60019 20020 80022 500

r maximum14.63cm(5.75 in)

163367653

1 0201 4702 0002 6103 3004 080

4 9505 8706 8908 0009 18010 40011 80013 20014 70016 30018 00019 70021 70023 50025 50027 600

K Factor

11 5 00051 10028 80018 40012 8009 8907 1905 6804 6003 8003 2002 7202 3502 0401 8001 600

1 4201 2701 1501 040

950869799736680

Table 1-10. GS-3: RCF and K Factor


1 0001 5002 0002 5003 0003 5004 0004 5005 0005 5006 0006 5007 0007 5008 0008 5009 000

RCFr minimum10.47 cm(4.12in)

117263468731

1 0501 4301 8702 3702 9203 5404 2104 9405 7306 5807 4908 4509 470

r average12.80 cm(5.04 in)

143322572894

1 2901 7502 2902 9003 5804 3305 1506 0407 0108 0509 15010 30011 600

r maximum15.14 cm(5.96 in)

169381677

1 0601 5202 0702 7103 4304 2305 1206 0907 1508 2909 51010 80012 20013 700

K Factor

93 40041 50023 40014 90010 4007 6305 8404 6103 7403 0902 6002 2101 9101 6601 4601 2901 150

1-13



1 0001 5002 0002 5003 0003 5004 0004 5005 0005 5006 0006 5007 0007 5008 0008 5009 0009 50010 00010 50011 00011 50012 00012 50013 00013 50014 00014 50015 00015 50016 00016 500

Table 1-11r minimum7.93 cm(3.12 in)

89199354553792

1 0801 4201 7902 2102 6803 1903 7404 3404 9805 6706 4007 1707 9908 8509 76010 70011 70012 80013 80015 00016 10017 40018 60019 90021 30022 70024 100

. SA-600: RCF and K Factorr average10.44 cm(4.11 in)

117262467729

1 0501 4301 8702 3702 9203 5304 2004 9305 7206 5607 4708 4309 45010 60011 70012 90014 10015 40016 80018 20019 70021 30022 90024 50026 20028 00029 90031 800

r maximum12.95 cm(5.10in)

145326579905

1 3001 7702 3202 9303 6104 3805 2106 1207 0908 1409 26010 50011 70013 10014 50016 00017 50019 10020 80022 60024 50026 40028 40030 40032 60034 80037 10039 400

K Factor

124 00055 30031 10019 90013 80010 1007 7706 1404 9704 1103 4502 9402 5402 2101 9401 7201 5301 3801 2401 1301 030940863796736682634591552517486457

1-14


Table 1-12. SE-12 Rotor: RCF and K Factor


1 0001 5002 0002 5003 0003 5004 0004 5005 0005 5006 0006 5007 0007 5008 0008 5009 0009 50010 00010 50011 00011 50012 00012 50013 00013 50014 00014 50015 00015 50016 00016 50017 00017 50018 00018 50019 00019 50020 000

RCFr minimum5.21 cm(2.05in)

58131233364524713931

1 1801 4501 7602 0902 4602 8503 2703 7204 2004 7105 2505 8206 4107 0407 6908 3809 0909 83010 60011 45012 20013 10014 00014 90015 90016 80017 80018 90019 90021 00022 10023 300


81182324506729993

1 3001 6402 0302 4502 9203 4203 9704 5605 1905 8506 5607 3108 1008 9309 80010 70011 70012 70013 70014 80015 90017 00018 20019 50020 70022 10023 40024 80026 30027 70029 30030 80032 400


104234416649935

1 2701 6602 1002 6003 1403 7404 3905 0905 8406 6507 5008 4109 37010 40011 50012 60013 80015 00010 20017 60018 90020 45021 80023 45025 00026 60028 30030 00031 90033 70035 50037 50039 50041 500

K Factor

147 00065 20036 70023 50016 30012 0009 1707 2405 8704 8504 0703 4703 0002 6102 2902 0301 8101 6201 4701 3301 2101 1101 020939868805748698652610573539507471453429400386367

1-15


Table 1-13. SM-24 Rotor: RCF and K Factor


1 0001 5002 0002,5003 0003 5004 0004 5005 0005 5006 0006 5007 0007 5008 0008 5009 0009 50010 00010 50011 00011 50012 00012 50013 00013 50014 00014 50015 00015 50016 00016 50017 00017 50018 00018 50019 00019 50020 000

NNER Rbw1

RCF

r minimum4.83 cm(1.90 in)

54121216337485661863

1 0901 3501 6301 9402 2802 6403 0303 4503 9004 3704 8705 3905 9406 5207 1307 7608 4309 1109 83010 60011 30012 10013 00013 80014 70015 60016 50017 50018 50019 50020 50021 600


77172307479690939

1 2301 5501 9202 3202 7603 2403 7504 3104 9005 5406 2106 9207 6608 450921010 1001 1 00012 00012 90014 00015 00016 10017 20018 40019 60020 90022 10023 50024 80026 20027 70029 10030 750


99224398621894

1 2201 5902 0102 4803 0003 5804 2004 8705 5906 3607 1808 0508 9609 93011 00012 00013 10014 30015 50016 80018 10019 50020 90022 30023 90025 40027 00028 70030 40032 20034 00035 90037 80039 700

K Factor

1 55 00068 70038 60024 80017 20012 6009 6607 6306 1805 1104 2903 6603 1502 7502 4202 1401 9101 7101 5501 4001 2801 1701 070989915848789735687643604568535505477452428407386

OUTER ROW

RCF


78176312488702956

1 2501 5801 9502 3602 8103 3003 8204 3904 9905 6406 3207 0407 8008 6009 44010 30011 20012 20013 20014 20015 30016 40017 60018 80020 00021 20022 60023 90025 30026 70028 20029 70031 200


101226402629906

1 2301 6102 0402 5203 0403 6214 2504 9305 6606 4407 2708 1509 080

10 10011 10012 20013 30014 50015 80017 00018 30019 70021 20022 60024 20025 80027 40029 10030 80032 60034 40036 30038 30040 200


123277493770

1 1101 5101 9702 4903 0803 7304 4305 2006 0406 9307 8808 9009 98011 10012 30013 60014 90016 30017 70019 20020 80022 40024 10025 90027 70029 60031 50033 50035 60037 70039 90042 20044 50046 80049 300

K Factor

115 00051 30028 90018 50012 8009 4207 2105 7004 6203 82032102 7302 3602 0501 8001 6001 4301 2801 1501 050954873802739683633589549513481451424399377356337320304289

1-16


Table 1-14. SS-34 Rotor: RCF and KFactor


1 0001 5002 0002 5003 0003 5004 0004 5005 0005 5006 0006 5007 0007 5008 0008 5009 0009 50010 00010 50011 00011 50012 00012 50013 00013 50014 00014 50015 00015 50016 00016 50017 00017 50018 00018 50019 00019 50020 000

RCF


64144255399575782

1 0201 2901 6001 9302 3002 7003 1303 5904 0904610

5 1705 7606 3907 0407 7308 4409 2009 98010 80011 60012 50013 40014 40015 30016 30017 40018 50019 60020 70021 90023 10024 30025 500


92208369577830

1 1301 4801 8702 3102 7903 3203 9004 5205 1905 9006 6607 4708 3209 22010 20011 20012 20013 20014 40015 60016 80018 10019 40020 80022 20023 60025 10026 70028 20029 90031 60033 30035 10036 900


121271483754

1 0901 4801 9302 4403 0203 6504 3405 1005 9106 7807 7208 7109 77010 90012 10013 30014 60016 00017 40018 80020 40022 00023 60025 40027 10029 00030 90032 80034 90036 90039 10041 30043 50045 90048 200

K Factor

161 00071 50040 20025 70017 90013 10010 1007 9506 4505 3204 4703 8103 2802 8602 5102 2301 9901 7801 6101 4601 6301 2201 1201 030952883821765715670629591557525497470446423402

1-17


1-6. CRITICAL ANGULAR VELOCITY (Critical "Speed").

The critical "speed" is that "speed" at which any rotor imbalance will produce a driving frequencyequal to the resonant frequency of the rotating system (i.e., rotor, and the centrifuge drive).At this "speed", the rotor exhibits large amplitude vibrations which can be felt in the instrumentframe. Mass imbalance will contribute to increased vibration intensity at the critical "speed" range.Good operating procedure is to avoid operation at the critical "speed" range.

Critical "speed" ranges for the superspeed angle rotors are given in Table 1-15.

CAUTION

Continued operation at the critical "speed"range will have a detrimental effect on centri-fuge component life.

Table 1-15. Critical Angular Velocityof Superspeed Angle Rotors

(in rev/min)

GSA

GS-3

SA-600

SE-12

SM-24

SS-34

RC-2B, RC-5, RC-5B

800

—

950

1100

1100

1140

SS-3

800

600

950

1100

1100

1140

1-18


Section 2. OPERATION2-1 . PRE-RUN CHECKS.

Before every run, make a quick check of the following to ensure optimum safety performance:

a. Check that the tube cavities are free of corrosion.

b. Check that the tapered mounting surface is clean.

WARNING

Every part of a centrifuge rotor should bekept scrupulously clean. It should becarefully inspected by the user prior to everyrun. If inspection reveals any sign of corrosionor cracking, rotor should be withdrawn fromservice.

2-2. TUBE, BOTTLE AND ADAPTER ASSEMBLY AND INSTALLATION.

Refer to the assembly and installation instructions which accompany the tube, bottle, and/oradapter used.

Tubes or bottles may be installed either before or after the rotor is placed in the centrifuge perthe rotor balancing instructions in paragraph 2-3.

2-3. ROTOR MOUNTING AND BALANCING.

a. Mounting

Prior to mounting the rotor on the tapered spindle of the centrifuge rotor drive, insure that therotor center hole and tapered spindle are free of foreign materials, nicks, and scratches. Wipe surfacesclean before each seating operation to minimize rotor sticking, scratching, and corrosion. Place therotor on the tapered spindle and carefully engage the drive pins.

If centrifuge temperature is below room temperature, and unless the rotor has been pre-chilled, allow time for the rotor to cool to the lower temperature before clamping the rotor in placeto preclude the rotor sticking to the spindle. Relative motion of the rotor with respect to the spindlemay wedge the rotor on the spindle as it contracts with decreasing temperature.

All superspeed angle rotors are equipped with double locking screws on the cover. The largerhand screw secures the cover to the rotor and the smaller hand screw secures the rotor to the taperedspindle. Tighten each screw separately — first the larger screw, then the smaller.

2-1


b. Balancing

For best performance, each rotor compartment load, including adapters (where applicable),tubes, and specimen must be properly balanced within 3 to 5 grams. Improper rotor balance maydamage the rotor drive. For a fully loaded rotor, a visual check of tube contents is generally sufficient toprove the rotor is balanced.

Do not attempt to operate rotors with massesthat differ by more than five grams in opposingcompartments.

If less than a full complement of specimen tubes is being run, place them in opposite compart-ments. Water-filled tubes may be used to balance the rotor as required. Balance each opposing pairindividually, as shown in figure 2-1.

Six Place Rotor(GSA, GS-3)

Eight Place Rotor(SS-34)

Twelve Place Rotor(SA-600, SE-12)

Twenty-four Place Rotor(SM-24)

6/7-1SA/10

Figure 2-1. Positioning Pairs of Tubes or Bottles in the Rotor

2-2


2-4. ROTOR "SPEED'VTEMPERATURE DIFFERENTIAL DETERMINATION.

Rotor "speed'Vtemperature differential graphs and tables, such as figure 2-2, are supplied withthe instruction manual for most centrifuges. These are only an approximate guide showing thetemperature run control setting required to maintain desired sample temperature in the rotorbeing used. The curves are approximate since the temperature offset (difference between indicatedand sample temperature) depends upon instrument efficiency and location, ambient temperature,type of rotor and rotor "speed". When sample temperature is critical, the required offset should bedetermined for each specific run.

CAUTION

The temperature offset technique should beused on all runs at either low or high "speeds"to prevent overtemperature and/or freezingof sample.

To plot a correction curve and create graphs for each rotor for an individual instrument, it isnecessary to plot the set temperature versus the actual sample temperature for the rotor used at aspecific operating "speed" and ambient temperature.

Example: To derive a +7.5 °C sample temperature with a SE-1 2 rotor at a speed of 1 5 000 rev/minin an RC-5 centrifuge in an ambient temperature of 25 °C, it is necessary to set the blue set tempera-ture at approximately +5 °C, or 2.5 °C colder than the desired sample temperature.

o

LU

en

ECLUQ-

LU

LU_ l0 .

<C/)

EQUILIBRIUM TIME=90MIN@SET TEMPERATURE

SET TEMPERATURE (°C)

Figure 2-2. Sample Rotor "Speed"/Temperature Differential Chart

2-3

superspeed Angle Rotors DuPont Instruments

NOTE

An ambient temperature of 25 °C or less isrequired to obtain optimum cooling efficiency.At higher ambient temperatures, lower oper-ating "speed" to maintain sample temperaturemay be necessary.

Correction curve data can be obtained and plotted in a test run by dynamically calibrating a rotor/centrifuge/desired speed combination for an actual run. Using an immersable centigradethermometer calibrated in 0.1 °C increments, and performing the following sequence, the result willbe a more accurate temperature correction for that rotor/centrifuge combination at that specific"speed" and ambient temperature.

NOTE

Blank rotor "speed'Vtemperature differentialcharts are provided in the back of this manual.

a. Set temperature to the desired sample temperature.

b. Prepare two opposing compartments with dispensable fluid (compatible with aluminum andwith a freezing point somewhat below sample temperature described) per rotor balancing instructionsdescribed in Section 2-3.

c. Pre-cool the rotor in the centrifuge as per instrument operating instructions.

d. Pre-cool the thermometer in a refrigerator to 1.0 °C below desired sample temperatureestablished in step (1) above.

e. When pre-cool time has elapsed, mount the rotor in the centrifuge and run at the speed forwhich the offset is to be determined for at least 1/2 hour.

f. When run time has elapsed and the rotor has stopped, open the door, quickly open acompartment and immerse the pre-cooled thermometer into the liquid. Agitate the thermometer inthe liquid for approximately five to ten seconds and record the temperature indication.

g. The desired temperature for the actual run is obtained by resetting the blue set temperatureupward or downward from the indication recorded on the thermometer.

Example: If the recorded indication is 2 °C warmer than that desired, reset set temperaturedownward 2 °C.

h. Record the data on the appropriate Rotor "Speed'VTemperature Differential Chart for futureuse.

2-4


2-5. REDUCING "SPEEDS" FOR DENSE FLUIDS.

There is a recommended design mass established for each superspeed angle rotor, representingthe maximum mass which should be carried in each compartment. The total package of contents foreach compartment, including specimen, tubes, cover, and adapter (if used), should not exceed therecommended figure given in Table 2-1 unless rotor "speed" is lowered proportionately.

Angular Velocity = maximum rev/min(from Table 2-1)

X/Design Mass (from Table 2-1Actual Compartment Mass

CAUTION

Solubility limits of gradients at the operatingtemperature should never be exceeded.

The structural parts of the rotor are made ofaluminum alloy. For this reason it is not in-tended for use with strong acids, strong basesor with the salts of heavy metals such ascesium, lead, silver or mercury.

Table 2 -1 . Recommended Design Mass perCompartment for Each Rotor

Rotor

SS-34SE-12SM-24GSAGS-3SA-600

Design Mass perCompartment

(9)

1153027

580780115

MaximumAngular Velocity

(rev/min)

20 00020 00020 00013 0009 000

16 500

100

10 30 40 50 60 70

Percentage Over Design Mass

80 90 100

2-5

Superspeed Angle Rotors Qll Pont Instruments

2-6. ESTIMATION OF SEDIMENTATION TIMES IN AQUEOUS(NON-GRADIENT) SOLUTIONS.

The time required to sediment a particle in water at 20 °C through the maximum rotor path length(i.e. the distance between r minimum and r maximum) can be estimated using the equation

Kt = -=

where t = sedimentation time in hoursK = the clearing factor for the rotor (defined below)

expressed in hour-Svedbergs*

S20, u> — the sedimentation coefficient for the particle of in-terest in water at 20 °C as expressed in Svedbergs*

The clearing, or K factor, is defined by the equation

if f ? n m m I i.L lx maximum \ . /rotor angular velocityVK — (253 300) In I 7 — I — I i r t r . r i I

I \ r minimum / \ 1000 /

Where r maximum and r minimum are the maximum and minimumrotor radii, respectively, and rotor angular velocity ("speed") isexpressed in rev/min.

K factors for the superspeed angle rotors for "speeds" from 1000 rev/min to maximum rev/minhave been listed in Tables 1-9 through 1-14.

EXAMPLE

The SS-34 rotor has a K factor of 402 at the maximum permitted "speed" (20 000 rev/min). Ifthe particles to be sedimented have a sedimentation coefficient of 100 S, the estimated run timerequired at maximum "speed" will be

402t = = 4.02 hours = 4 hours, 1 min

Note that the calculation assumes particles in waterat 20 °C; if the suspending medium isdenseror more viscous than water, the sedimentation time will be greater.

dx 1 d x

*The sedimentation coefficient (s) in seconds, for a centrifugal field is defined by the equation s = ^ 2 , where -^ =sedimentation velocity of the particle in cm/s; a>= rotor "speed" in radians/s; and x = the distance of the particle from the axis of rotationin centimeters. Conventionally, experimentally determined values of sedimentation coefficients are multiplied by 10" to convert them toSvedberg units (S), so named in honor of the pioneer in ultracentrif ugation, The Svedberg. Thus a particle with an experimentally determinedsedimentation coefficient of 1 0 " seconds is usually referred to in the literature as "100 S particle". Since the value determined for thesedimentation coefficient is dependent on the density and viscosity of the solution in which centrifugation is performed, values are usuallyreported for the standard conditions of infinite dilution in water at 20 °C, and designated S2o,«u.

2-6


Section 3. MAINTENANCE

3-1. CORROSION.

The precision-engineered rotor is made from aluminum alloy for a high strength-to-weight ratio.Although its corrosion resistance is good, it is not as good as stainless steel or titanium. Therefore,exercise greater care in its maintenance. With a few precautions, you can minimize corrosion andsignificantly prolong the useful life of the rotor. A little care, conscientiously applied, may precluderotor failure and its potential damage to the centrifuge.

Generally, corrosion refers to chemical reactions at the surface; rusting or pitting signaled bygrowing areas of visible deterioration. On the other hand, stress corrosion attacks the inside of themetal as well. Barely detectable cracks on the surface grow larger inward, weakening the part withouta visible warning.

Stress corrosion is thought to be initiated by certain combinations of stresses and chemicalreactions. The most common chemical causing deleterious effects is the chloride ion, whether insolution (ammonium salts) or in as subtle a form as the sodium chloride in perspiration. If thesechemically harmful substances are allowed to remain on the rotor, corrosion almost certainly willresult.

The normal environment of a working rotor is a moist atmosphere, through normal humidity orbecause the rotor is cooled belowthe dew point in a cold room or in a refrigerated centrifuge. Moistureof any sort can initiate corrosion. Therefore, the rotor should be kept dry when not in use, and tubesshould be removed from the compartments after each run prior to storage. Corrosion often startsunder tubes left in compartments, particularly if condensation develops.

Stress, such as high centrifugal force, with corrosive conditions, will usually accelerate thecorrosive process in an exponential time relationship; i.e., time to failure, or useful life, tends todecrease rapidly as stresses increase. Thus, rotor condition, speed, and load demand equal attention.

Under appropriate conditions, the stress corrosion applies to almost all commonly used alloys.Even the corrosion-resistant alloys have been found susceptible. Therefore, be aware that favorableconditions for stress corrosion cracking are present in all rotor applications and that care and attentionare required to minimize its effects.

3-2. WASHING.

Wash the rotor with warm water and a mild soap or detergent at least once a week or, ideally, aftereach use. It is particularly important to wash the rotor immediately after any spills have occurred. Mostlaboratory chemicals can be removed with a lukewarm, 1 % solution of a mild, non-alkaline detergentsuch as Ivory Liquid. Rinse the rotor well, inside and out. Ordinary tap water is usually adequate, but

3-1

Superspeed Angle Rotors DU POIIt Instruments

distilled water is preferable, especially for the last rinse. After rinsing, dry thoroughly, especially thecavities. Use a soft absorbent cloth or towel or an air blast.

CAUTION

To avoid damaging the rotor surfaces whencleaning:

• Do not use strong laboratory detergents.

• Use a stiff bristle brush only as required toloosen encrusted materials, but be careful notto scratch the surfaces with the wire tip.

• Temperature must not exceed 100 °C at anytime. Use ethylene oxide not autoclaving tosterilize rotor body.

3-3. CONTAMINATION.

Because of the nature of samples likely to be processed in the rotor, the chance of contamina-tion, either biological or radioactive, is very possible. Always be aware of this possibility and takenormal precautions. Use standard decontamination procedures should exposure occur.

Should a centrifuge or rotor that has been used with radioactive or pathogenic material requireservicing by Du Pont personnel either at the customer's laboratory or at Du Pont facilities, complywith the following procedure to ensure safety of our personnel.

a. Clean the centrifuge and/or rotor to be serviced of all encrusted material and decontaminateit prior to servicing by our representative. There must be no radioactivity detectable by surveyequipment.

b. Complete and attach Decontamination Information Certificate (Du Pont Instruments FormNo. IPDP-59) to the centrifuge or rotor.

If centrifuge or rotor to be serviced does not have a Decontamination Information Certificateattached and, in our opinion, presents a potential radioactive or biological hazard, our represe-entative will not service the equipment until proper decontamination and certification is complete.If we receive a centrifuge or rotor at our Service facilities which, in our opinion, is a radioactive orbiological hazard, the sender will be contacted for instructions as to disposition of the equipment.Disposition costs will be borne by the sender.

Decontamination Information Certificates are included with these instructions. Additionalcertificates are available from your local technical or customer service representative. In the eventthese certificates are not available, a written statement certifying that the unit has been properlydecontaminated and outlining the procedures used will be acceptable.

NOTE

Service representative wil l annotate aCustomer Service Repair Report if decontam-ination was required, and if so, what thecontamination was and what procedure wasused. If no decontamination was required, itwill be so stated.

3-2

DU POnt Instruments Superspeed Angle Rotors

3-4. STORAGE.

It is recommended that rotors be stored (after rinsing and drying) upside-down, with caps, plugs,and tubes removed, so air can circulate. This will assist in preventing moisture from gathering andsettling at the bottom of the tube compartments.

3-3/3-4


+30 4----J

LU

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-20

30 - -+- -

+20 +10 +5 0 -5 -10


Rotor "Speed'VTemperature Differential Chart for theRotor

15

Ambient Temperature -

Rotor "Speed"


+30

+20

+10

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tti

::±±:* • -

±

•10

-20

•30

ffl 1 :±tttI

+20 +15 +10 +5 0 -5 10



•20

Ambient Temperature _

Rotor "Speed"


•-t

mix

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DU PONT COMPANY INSTRUMENT PRODUCTSNEWTOWN, CT 06470

(203) 426-5811

BIOMEDICAL DIVISION

District Offices:

WILMINGTON, DE 19898Quillen Bldg., Concord Plaza(302) 772-5655

ROCKVILLE, MD 2085211900 Parklawn Drive(301)770-2500

DES PLAINES, IL 600182200 E. Devon Ave.(312)298-4555

CLAREMQNT, CA 91711675 West Foot Hill Blvd.(714) 624-9007

DuPont Instruments

IPDP-406

Printed in U.S.A.

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