Instruction Manual for the
ibidi Heating System 1, Universal Fit
Version 2.1
ibidi Heating System 1, Instruction Manual
II ©ibidi GmbH 2012, version 2.1, revision 1, 2012-12-03, Dr. Miro Kroutvar, Dr. Zeno Guttenberg
ibidi Heating System 1, Instruction Manual
© ibidi GmbH 2012, version 2.1, revision 1, 2012-10-09, Dr. Miro Kroutvar, Dr. Zeno Guttenberg 1
Dear User,
Live cell imaging enhances the power of cell microscopy, immensely. Intracellular transport
phenomena can be tracked with high-resolution fluorescence microscopy. In addition, the
low-magnification microscopy of living cells, such as is used for migration or proliferation
experiments, is a topic of great interest. From these examples, you can appreciate the wide
variety of optical microscope setup requirements that are often used in one lab.
So, when considering a design for the ibidi Heating System, our objective was to create a
microenvironment for cell culture and microscopy that would be compatible with every type
of inverted microscope, regardless of the brand.
We then created the following list of requirements for the new heating system, based on
customer needs and the industry standards:
• Stable conditions for temperature, humidity, CO2, and O2
• Compatible with any inverted microscope
• Suitable for, but not limited to, use with all ibidi µ-Slides and µ-Dishes
• Usable with all objective lenses (up to 100x oil)
• Portable from one microscope to another
• Easy handling
• Reasonable pricing
• Short installation time
• Reversible; no modification to your microscope required
• Usable within an incubated microscope
After considering these issues, we came up with the idea to search for a standard format
that could be used on all inverted microscopes. Our solution was simple: a multi-well format.
There are a large numbers of plates currently in use (e.g., 12 well, 24 well, 96 well, etc.) and
all of them have the same outer dimensions: 127.5 mm x 85.5 mm. This means that for
every inverted microscope, there is a stage or a holder in the same format. This type of
holderbecame the basis for all of the microscope platforms.
Using this multi-well idea, we designed a heating system that is capable of meeting all of
our requirements. Universal usage is just one of the system’s outstanding features. We also
developed a heated lid that prevents water condensation effects and provides stable
temperature conditions. Finally, controls for CO2, O2, and humidity complete the system.
Thank you for purchasing the ibidi Heating System. We wish you success with it, and we
welcome your feedback!
ibidi Heating System 1, Instruction Manual
2 ©ibidi GmbH 2012, version 2.1, revision 1, 2012-12-03, Dr. Miro Kroutvar, Dr. Zeno Guttenberg
Table of contents
Safety Considerations............................................................................................................ 4
Regulatory Statement............................................................................................................ 7
Specifications ........................................................................................................................ 8
Preface .................................................................................................................................10
Notices .................................................................................................................................10
Limited Warranty ..................................................................................................................11
Installation Requirements .....................................................................................................12
Installation and Connecting Cables .......................................................................................13
Maintenance and Operation .................................................................................................14
Waste Treatment .................................................................................................................15
1 Working Principle ..........................................................................................................16
2 Equipment .....................................................................................................................17
2.1 Product Description ..............................................................................................17
2.2 Heated Lid ............................................................................................................17
2.3 Heated Plate and Inserts .......................................................................................18
2.4 Sterilization and Cleaning ......................................................................................19
3 ibidi Temperature Controller ..........................................................................................20
3.1 Connecting the Temperature Controller ................................................................21
3.2 Menu Navigation ...................................................................................................22
3.3 Supply Voltage Adjustments .................................................................................22
3.4 Setting into Operation ...........................................................................................22
4 Temperature Calibration ................................................................................................24
4.1 Calibration Routine ................................................................................................24
4.2 Influence of Surrounding Temperature and Airflow ...............................................25
5 TempControl Software ..................................................................................................27
5.1 Software Overview ...............................................................................................27
5.2 Logging Menu .......................................................................................................29
5.3 Advanced Menu ....................................................................................................29
5.3.1 Calibration Routine Using TempControl .............................................................30
6 Troubleshooting ............................................................................................................31
6.1 Focus Not Stable ...................................................................................................31
6.2 Evaporation Too High ............................................................................................31
6.2.1 Parafilm Procedure ............................................................................................31
6.2.2 Silicone Oil Procedure (ibidi’s Anti-Evaporation Oil) ...........................................31
7 WEEE/RoHS Compliance Statement .............................................................................33
A Menu Navigation ............................................................................................................. I
A.1 Setting the Temperature ......................................................................................... I
A.2 External Temperature Sensor ................................................................................. II
ibidi Heating System 1, Instruction Manual
© ibidi GmbH 2012, version 2.1, revision 1, 2012-10-09, Dr. Miro Kroutvar, Dr. Zeno Guttenberg 3
A.3 Run / Stop Button .................................................................................................. II
A.4 Setup Button ..........................................................................................................III
A.4.1 Mode Function ...................................................................................................III
A.4.2 Alarm Function .................................................................................................. IV
A.4.3 Preferences ........................................................................................................ V
A.4.4 Calibration .......................................................................................................... V
A.4.5 Info ................................................................................................................... VI
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4 ©ibidi GmbH 2012, version 2.1, revision 1, 2012-12-03, Dr. Miro Kroutvar, Dr. Zeno Guttenberg
Safety Considerations
To ensure operation safety, the ibidi heating system must be operated correctly and
maintained according to a regular schedule. Carefully read to fully understand all safety
precautions in this manual before operating the instrument. Please take a moment to
understand what the signal words WARNING!, CAUTION and NOTE mean in this manual.
Safety symbols
WARNING! A WARNING! indicates a potentially hazardous situation which,
if not avoided, could result in serious injury or even death.
CAUTION A CAUTION indicates a potentially hazardous situation which, if
not avoided, may result in minor or moderate injury. It may also
be used alert against damaging the equipment or the
instrument.
Do not proceed beyond a WARNING! or CAUTION notice until
you understand the hazardous conditions and have taken the
appropriate steps.
NOTE A NOTE provides additional information to help the operator
achieve optimal instrument and assay performance.
Read manual label. This label indicates that you have to read
the manual before using the instrument. This label is positioned
at the backside of the temperature controller.
This symbol indicates important notes.
Identification label. This label is positioned at the
backside / rear panel of the temperature controller.
ibidi Heating System 1, Instruction Manual
© ibidi GmbH 2012, version 2.1, revision 1, 2012-10-09, Dr. Miro Kroutvar, Dr. Zeno Guttenberg 5
Nomenclature
TEMPERATURE HEATED LID HEATED PLATE
CONTROLLER
WARNING! Do only operate the ibidi heating system with the delivered cables and plugs. If
not doing so you risk electric shock and fire.
WARNING! Do not operate the ibidi heating system with substances and under conditions
which do cause a risk of explosion, implosion or release of gases. Only operate the ibidi
heating system with aqueous solutions.
CAUTION Ensure that the power plug is well accessible. The ibidi heating system has to be
installed in a way that it does not hinder the access to the power plug.
CAUTION Operate the heating system only on a power socket with protective conductor to
ground the housing of the temperature controller.
CAUTION The weight of the ibidi heating controller is approx. 3.4 kg. Provide a secure stand
for controller, where it cannot be accidentally dropped. Moving the heating system poses a
risk of personal injury or damage to the instrument.
CAUTION The manual opening of the temperature controller is not allowed. Manual opening
pose a risk of personal injury or damage to the controller. Contact ibidi service personnel if
you need to open the controller.
CAUTION The ibidi heating system includes a heated plate with heated lid to control the
sample temperatures. Some accessible parts of the plate and lid can reach temperatures up
to 55°C. Avoid touching the temperature controlled parts of the system when you have set
the temperature controller to high temperatures.
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6 ©ibidi GmbH 2012, version 2.1, revision 1, 2012-12-03, Dr. Miro Kroutvar, Dr. Zeno Guttenberg
CAUTION When the glass plate of the heated lid was broken due to mechanical impact, the
shards can lead to injuries if touched.
CAUTION Be aware that when the system is switched on, 10 V AC voltage is applied to the
underside of the glass plate. Do not touch the underside or contact it with anything
conductive. This could cause a short circuit that may destroy the temperature controller
and/or the lid.
CAUTION Only use correct replacement fuses. The correct values of the replacement fuses
are indicated on the backside of the heating controller. Using wrong replacement fuses can
lead to overheating of the temperature controller which can cause fires.
CAUTION Only ibidi staff is allowed for servicing and opening of the temperature controller.
Turn off the power switch and unplug the power cord before servicing the system, unless
otherwise noted. Connect the temperature controller only to the delivered power plug. Do
not use extension cords. Have an electrician immediately replace any damaged cords, plugs,
or cables. Not doing so poses a risk of personal injury or damage to the system.
CAUTION Do only use the heating system in dry rooms (and do not use the heating system
in the cold room.
CAUTION Turn off the power switch in the back of the chassis, when the heating system is
not in use.
CAUTION Do not use ethanol or other types of organic solvents to clean the heating system
as they may remove the paint.
CAUTION Use the instrument only for cell experiments with aqueous solutions.
CAUTION Do not use the instrument with hazardous substances or substances/materials
which pose a risk of infections.
ibidi Heating System 1, Instruction Manual
© ibidi GmbH 2012, version 2.1, revision 1, 2012-10-09, Dr. Miro Kroutvar, Dr. Zeno Guttenberg 7
Regulatory Statement
in preparation
The following safety and electromagnetic standards were considered:
• IEC 61010-1:2001 Safety requirements for electrical equipment for measurement,
control and laboratory use. Part 1 General Requirements
• IEC 61010-2-010:2005 Safety requirements for electrical equipment for
measurement, control and laboratory use. Part 2-010: Particular requirements for
laboratory equipment for the heating of materials.
• IEC 61010-2-081:2001 Safety requirements for electrical equipment for
measurement, control and laboratory use. Part 2-081: Particular requirements for
automatic and semi-automatic laboratory equipment for analysis and other purposes.
• IEC 61326-1:2006 EMC, Electrical equipment for measurement, control and
laboratory use – EMC requirements.
• IEC 61000-3-2:2006 EMC, Limits for harmonic current emissions (equipment input
current up to and including 16A per phase).
• IEC 61000-3-3:2008 EMC, Limits
ibidi Heating System 1, Instruction Manual
8 ©ibidi GmbH 2012, version 2.1, revision 1, 2012-12-03, Dr. Miro Kroutvar, Dr. Zeno Guttenberg
Specifications Heating System 1
Power Consumption
Supply voltage 230 V / 50 Hz - 115 V / 60 Hz
Output voltage 10 – 40 V
Max. power input 160 VA
Max. power output 160 VA
Surrounding Conditions
Storage Temperature -5 – 50°C
Operating Temperature 15 - 40°C (Indoor use only)
Humidity 80 % relative humidity up to 31°C,
30 % relative humidity up to 40°C
Operating altitude max. 2000 m
Temperature Control Range Ambient temperature to +55°C
Net Dimensions (hxwxd)
Controller 90 x 170 x 230 mm³
Heated Plate with Lid 25.5 x 125.5 x 85.5 mm³
Weight
Controller 3.4 kg
Heated Plate with Lid 210 g
Fuses DIN 41661 (5 x 20 mm)
230 V / 50 Hz 1 A (T)
115 V / 60 Hz 2 A (T)
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© ibidi GmbH 2012, version 2.1, revision 1, 2012-10-09, Dr. Miro Kroutvar, Dr. Zeno Guttenberg 9
Connections to the temperature controller
All ingoing and outgoing connections can be found at the rear panel of the instrument.
Name Function
USB cable
Power Switch
Power cable
Fuse box
External sensors
Control channels 1-4
Socket for connecting the USB cable to the PC/Notebook. To
setup a computer communication to the ibidi temperature
controller, the USB cable must be connected.
When the switch is pressed on (position “I”), the temperature
controller is switched on.
The connector to the electrical power supply
Contains the fuses for the temperature controller
Ports for external temperature sensors (four terminal
sensing of Pt100 resistors and thermocouple sensors)
4 connectors for the 4 control channels
ibidi Heating System 1, Instruction Manual
10 ©ibidi GmbH 2012, version 2.1, revision 1, 2012-12-03, Dr. Miro Kroutvar, Dr. Zeno Guttenberg
Preface
This manual is your guide for using the ibidi heating system for cell culture experiments on
an optical microscope. It instructs first-time users on how to use the instrument, and serves
as a reference for experienced users.
Before using the ibidi heating system, please read this instruction manual carefully, and
make sure that the contents are fully understood. This manual should be easily accessible to
the operator at all times during instrument operation. When not using the instrument, keep
this manual in a safe place. If this manual becomes lost, order a replacement from ibidi
GmbH.
Notices
1. ibidi shall not be held liable, either directly or indirectly, for any consequential damage
incurred as a result of product use.
2. Prohibitions on the use of ibidi software
• Copying software for other than backup
• Transfer or licensing of the right to use software to a third party
• Disclosure of confidential information regarding software
• Modification of software
• Use of software on multiple workstations, network terminals, or by other
methods
3. The contents of this manual are subject to change without notice for product
improvement.
4. This manual is considered complete and accurate at publication.
5. This manual does not guarantee the validity of any patent rights or other rights.
6. If an ibidi software program has failed causing an error or improper operation, this
may be caused by a conflict from another program operating on the notebook (PC). In
this case, take corrective action by uninstalling the conflicting product(s).
7. ibidi is a registered trademark of ibidi GmbH in Germany and other countries.
ibidi Heating System 1, Instruction Manual
© ibidi GmbH 2012, version 2.1, revision 1, 2012-10-09, Dr. Miro Kroutvar, Dr. Zeno Guttenberg 11
Limited Warranty
Products sold by ibidi, unless otherwise specified, are warranted for a period of one year
from the date of shipment to be free of defects in materials and workmanship. If any
defects in the product are found during this warranty period, ibidi will repair or replace the
defective part(s) or product free of charge.
THIS WARRANTY DOES NOT APPLY TO DEFECTS RESULTING FROM THE FOLLOWING:
1. IMPROPER OR INADEQUATE INSTALLATION.
2. IMPROPER OR INADEQUATE OPERATION, MAINTENANCE, ADJUSTMENT OR
CALIBRATION.
3. UNAUTHORIZED MODIFICATION OR MISUSE.
4. USE OF UNAUTHORIZED CELL CULTURE WARE.
5. USE OF CONSUMABLES, DISPOSABLES AND PARTS NOT SUPPLIED BY AN
AUTHORIZED IBIDI DISTRIBUTOR.
6. CORROSION DUE TO THE USE OF IMPROPER SOLVENTS, SAMPLES, OR DUE TO
SURROUNDING GASES.
7. ACCIDENTS BEYOND IBIDI’S CONTROL, INCLUDING NATURAL DISASTERS.
This warranty does not cover consumables like cell culture dishes, reagents and the like.
The warranty for all parts supplied and repairs provided under this warranty expires on the
warranty expiration date of the original product. For inquiries concerning repair service,
contact ibidi after confirming the model name and serial number of your ibidi system.
ibidi Heating System 1, Instruction Manual
12 ©ibidi GmbH 2012, version 2.1, revision 1, 2012-12-03, Dr. Miro Kroutvar, Dr. Zeno Guttenberg
Installation Requirements
To ensure operation safety, observe the following conditions:
1. Do only operate the ibidi heating system with the delivered power cable.
2. Only connect the power cable of the ibidi heating system to an electrical socket
containing a protective conductor terminal.
3. Ensure that the power plug of the power supply is well accessible. The ibidi heating
system has to be installed in a way that it does not hinder the access to the external power
supply and its power plug.
4. Only operate the heating system with the delivered PC (Notebook).
5. Only operate the heating system with original ibidi insert.
6. Operate the heating system in a temperature range of 15 – 40°C.
7. Operate the heating system in a humidity range of 0 – 80% RH up to 31°C and 30% RH
up to 40 °C.
8. Operate the heating system in an atmospheric pressure range of 800 – 1060 hPa.
9. Do not operate the heating system under conditions which pose a risk of explosion,
implosion or the risk of the release of gases.
10. Avoid strong magnetic fields and sources of high frequency. The heating system may
not function properly when near a strong magnetic field or high frequency source.
11. Avoid vibrations from vacuum pumps, centrifuges, electric motors, processing
equipment and machine tools.
12. Avoid dust and corrosive gas. Do not install the heating system where it may by exposed
to dust, especially in locations exposed to outside air or ventilation outlets.
13. For cleaning the heating system, only use ultrapure water.
14. Do not install the heating system in a location where it may be exposed to direct
sunlight.
15. Install the heating system in a horizontal and stable position. This includes a table, bench
or desk upon which the instrument is installed.
16. Ensure that no air conditioner blows air directly onto the heating system. This may
prevent stable conditions.
17. Install the heating system in a location that allows easy access for maintenance.
NOTE: The above conditions do not guarantee optimal performance of this heating system.
ibidi Heating System 1, Instruction Manual
© ibidi GmbH 2012, version 2.1, revision 1, 2012-10-09, Dr. Miro Kroutvar, Dr. Zeno Guttenberg 13
Installation and Connecting Cables
Connecting the ibidi heating system to the power supply
Confirm that the power switch of the heating system is off (power switch is at the backside,
right of the temperature controller).
WARNING! Do only operate the ibidi heating system with the delivered cables and plugs. If
not doing so you risk electric shock and fire.
CAUTION Ensure that the power plug of the external power supply is well accessible. The
heating system has to be installed in a way that it does not hinder the access to its power
plug.
Connect the heating system to the Notebook by using the delivered network cable.
Switch on the heating system and the Notebook.
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14 ©ibidi GmbH 2012, version 2.1, revision 1, 2012-12-03, Dr. Miro Kroutvar, Dr. Zeno Guttenberg
Maintenance and Operation
Pay attention to the heating system operating environment and always keep it clean so that
the instrument can be used in a stabilized condition over a long period. Do not place
anything heavy on the temperature controller.
Cleaning the ibidi heating system
Switch off the heating system and remove the power plug from the electrical socket. Only
use dry cloth or cloth wetted with water (ultrapure) for cleaning of the instrument.
CAUTION Do not use ethanol or other types of organic solvents to clean the instrument as
they may remove the instrument paint.
Transporting the ibidi heating system
Switch off the temperature controller and remove the power plug from the electrical socket.
Carry the instrument carefully and avoid mechanical shocks.
CAUTION The weight of the temperature controller is approx. 3.4 kg. Dropping the
instrument while moving it, may cause personal injury or damage.
Functional Disorder
In case of a functional disorder switch off the temperature controller and wait for five
minutes, then switch on the instrument again. If there is still a functional disorder switch off
the device, unplug the power cable and contact the ibidi service.
Repairing the ibidi heating system
Do not try to repair the instrument by yourself. Do contact the ibidi service for repairing the
instrument.
CAUTION The manual opening of the instrument is not allowed. Manual opening pose a risk
of personal injury or damage to the instrument. Contact ibidi service personnel if you need to
open the instrument.
ibidi Heating System 1, Instruction Manual
© ibidi GmbH 2012, version 2.1, revision 1, 2012-10-09, Dr. Miro Kroutvar, Dr. Zeno Guttenberg 15
Waste Treatment
Waste treatment is your own responsibility. You must hand it to a company specialized in
waste recovery. Do not dispose the ibidi heating system in a litter bin or at a public waste
disposable site. For detailed information please contact the ibidi service.
ibidi Heating System 1, Instruction Manual
16 ©ibidi GmbH 2012, version 2.1, revision 1, 2012-12-03, Dr. Miro Kroutvar, Dr. Zeno Guttenberg
1 Working Principle
The stage top incubator consists of a heated plate, and also a heated lid that is designed to
keep cells at 37°C in microscopy slides and dishes. The heated lid prevents condensation
effects inside the entire incubation system. Humidity, CO2 enriched, and/or O2
reduced/enriched air can be flushed in, which makes the heated chamber a fully controlled
incubator—right on the microscope. This enables the user to perform live cell imaging.
The glass top of the lid and the bottom plate are actively heat-controlled. The heated plate
has the outer dimensions of a 96-well plate (127.5 mm x 85.5 mm).
Figure 2: Heated Lid and Heated Plate (without Insert)
Figure 1: Schematic Drawing of the ibidi Heating System
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© ibidi GmbH 2012, version 2.1, revision 1, 2012-10-09, Dr. Miro Kroutvar, Dr. Zeno Guttenberg 17
2 Equipment
Due to the height of the lid, we recommend using condensers with a working distance of
≥ 26 mm.
Information on gas incubation (CO2, O2, humidity) is provided in the Add-On instructions:
“Gas Incubation with Active Gas Mixer”.
2.1 Product Description
A brief overview is provided in this section, including the nomenclature for later descriptions
in this document.
Figure 3: Schematic Setup
2.2 Heated Lid
The heated lid (127.5 mm x 85.5 mm) provides excellent optical quality, and also allows for
the use of all standard microscopy techniques, including differential interference contrast
(DIC). The upper glass part is electrically heat controlled. The heated lid fits accurately to the
heated plate.
For gas incubation, the lid is equipped with inlets for (a) the gas flow and (b) the humidity
sensor.
Optional: PC
Figure 4: Heated Lid
Temperature
controller
Incubation
chamber
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18 ©ibidi GmbH 2012, version 2.1, revision 1, 2012-12-03, Dr. Miro Kroutvar, Dr. Zeno Guttenberg
2.3 Heated Plate and Inserts
The heated plate provides the base for the heating system. Its format (127.5 mm x 85.5
mm) fits every multi-well stage or multi-well holder (e.g., for 96-well plates) on the
microscope. It is fully heated with electricity. The heated plate is the counterpart to the
heated lid.
The two-part insert has two functions that both use the force of the integrated magnets. The
first function is to hold the µ-Slides or µ-Dishes firmly into position, so as to avoid
displacement during microscope stage movements. The second is to create a tight contact
of the µ-Slides or µ-Dishes to the metallic insert, which then maximizes the heat transfer and
ensures the stable heat control of the sample. The heated plate passively heats the inserts.
Figure 5: Heated Plate with Inserts
Heated plate, heated lid, and inserts are positioned by strong Neodym
Magnets! Please contact us for a non-magnetic system, in the case
that permanent magnetic fields will disturb your experiment!
ibidi Heating System 1, Instruction Manual
© ibidi GmbH 2012, version 2.1, revision 1, 2012-10-09, Dr. Miro Kroutvar, Dr. Zeno Guttenberg 19
2.4 Sterilization and Cleaning
The heated plate, the heated lid, and the inserts do not have to be sterile, because the
incubation chamber has no direct contact with the cells and the cell media.
However, all these parts can be cleaned. The inserts are very robust and can be cleaned
with Ethanol. The lid is made of polycarbonate and, hence, has only limited resistance
against alcohol. Therefore, we recommend using ultrapure water for cleaning. Also, the
bottom of the heated plate is not alcohol resistant, so this plate should not be cleaned with
Ethanol. Please use only the ultrapure water.
Do not clean lid and plate with alcohol! Use ultrapure water
(e.g., Millipore water) instead!
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20 ©ibidi GmbH 2012, version 2.1, revision 1, 2012-12-03, Dr. Miro Kroutvar, Dr. Zeno Guttenberg
3 ibidi Temperature Controller
The ibidi Temperature Controller is designed to precisely regulate the ibidi incubation
chamber. To achieve this, the controller has four control channels on the rear panel. When
required, the controller can be customized for two, or up to 64 channels. In addition, this unit
can be run as a standalone system or PC-controlled.
In Figure 6, you can see a picture of the controller. The measured (‘I’) and set (‘S’)
temperatures of all the channels are displayed. You can navigate through the menu by using
the control buttons on the right. The LEDs, located below the display, indicate the status of
the channels and feedback, the power, and the USB connection.
Figure 6: Front Panel of the ibidi Temperature Controller
Up /
rightbutton
Select /
confirmbutton
Down /
leftbutton
Control LEDs
LC display
Figure 6: Front Panel of the ibidi Temperature Controller
Up /
Rightbutton
Select /
Confirmbutton
Down /
Leftbutton
Control LEDs
LC display
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© ibidi GmbH 2012, version 2.1, revision 1, 2012-10-09, Dr. Miro Kroutvar, Dr. Zeno Guttenberg 21
3.1 Connecting the Temperature Controller
All plugs for the electrical connections are integrated into the rear of the ibidi Temperature
Controller, as shown in Figure . On the right side, you will find the plug for the power cable.
Above that is the power switch, and the fuse box is at the top. The USB cable connection is
located in the center of the box. There are ports for external temperature sensors (four-
terminal sensing of Pt100 resistors and thermocouple sensors). On the left side, you will
find the four connectors for the four control channels. In addition to these plugs, there are
upgrade options which you can find below.
The standard connectors are:
• power cable
• USB cable
• 4 control channels
• 2 external temperature sensors
Optional features:
• USB stick
• customized connector to for up to 64 control channels
• reduction to 2 control channels
To setup a computer communication to the ibidi Temperature Controller, the USB cable
must be connected.
Figure 7: Rear Panel of the ibidi Temperature Controller
Power
cable
Power
switch
Fusebox
USB cable
Controlchan
nels 1-4
Externals
ensors
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22 ©ibidi GmbH 2012, version 2.1, revision 1, 2012-12-03, Dr. Miro Kroutvar, Dr. Zeno Guttenberg
To calibrate the system for the sample temperature, you can use the external temperature
sensor of the controller or the control software TempControl. For more details, please refer
to Section 4.
3.2 Menu Navigation
Most control parameters can be manually set on the controller using the buttons and the LC
display on the front panel. The functions of the button are:
• up / right
• down / left
• select / confirm
The cursor position is indicated with square brackets (‘[xxx]’). You can move the cursor using
the ‘Left’ and ‘Right’ buttons. If you want to select a parameter or a function, you need to
press the ‘Select’ button. When you select a parameter, the square brackets will change to
angle brackets (‘<xxx>’). Now you are able to change the value up or down. To confirm the
changed value, you must press the ‘Confirm’ button once more. When selecting a function,
you will see the individual function view, which is where you can change the parameters or
access further functions.
You can find a detailed description of the menu navigation, including all functions, in
Appendix A.
3.3 Supply Voltage Adjustments
The control unit should only be used in dry rooms.
The control units must be adjusted to the corresponding voltage (230 V or 115 V). The actual
voltage setting may be read and adjusted at the integrated fuse box of the power cable
connector, located at the rear panel. In order to change these settings, it is necessary to pull
out the fuse holder from the casing. Replace the fuses to match the input voltage (1 A(T) for
230V / 50Hz or 2 A (T) for 115 V / 60 Hz). Rotate the grey fuse holder, and then reinsert it so
that the correct voltage is shown in the window. Snap the fuse box back into the housing.
The ‘On/Off’ switch is at the rear side of the unit. After selecting ‘On’, the controller will
automatically start a self-test, and then the measured values will be shown.
3.4 Setting into Operation
Here is the procedure to start the operation of the controller.
Be aware that when the system is switched on, 10V AC
voltage is applied to the underside of the glass plate. Do not
touch the underside or contact it with anything conductive.
This could cause a short circuit that may destroy the
controller and/or the lid.
ibidi Heating System 1, Instruction Manual
© ibidi GmbH 2012, version 2.1, revision 1, 2012-10-09, Dr. Miro Kroutvar, Dr. Zeno Guttenberg 23
For the first power up, we recommend disconnecting the heating devices (e.g., the heated
lid and the heated plate). By doing this, you ensure that you do not start heating with the
wrong temperature settings. It is now possible to set the temperatures for the individual
channels. Afterwards, you can reconnect the heating devices.
Since the controller is preconfigured, we recommend connecting the heated lid to Channel 1
and the heated plate to Channel 2. For subsequent power ups, you will not need to
disconnect the heating devices.
Please let the temperature of the system equilibrate for a
minimum of 30 minutes before you start your experiments.
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24 ©ibidi GmbH 2012, version 2.1, revision 1, 2012-12-03, Dr. Miro Kroutvar, Dr. Zeno Guttenberg
4 Temperature Calibration
Depending on the room temperature, air conditioning, heat loss via microscope, chamber
type or objective lens, and humidification, the temperature at the position of the cells has to
be calibrated from time to time. A calibration is recommended before beginning the first
experiment. The heat calibration should be done for each chamber type, in order to control
temperatures with an absolute accuracy of less than 1 °C.
For calibration, the ibidi Temperature Controller has a plug, located on the rear panel, for an
external temperature sensor. Before shipment, all controllers are run through an in-house
calibration to ensure their accuracy. This calibration only refers to the actively heated
devices, such as the plate and the lid. The samples are only indirectly heated via the insert.
As a result, the cell temperature will lie somewhat below the temperature of the heated
plate. To compensate for this effect, the heated plate can be calibrated to the cell
temperature that is sensed by the external temperature sensor, which can be placed at the
position of the cells.
4.1 Calibration Routine
The recommended calibration routine is fairly simple. Follow these eight steps for the initial
temperature calibration:
1) Plug the external temperature (thermo-couple type K) sensor into the rear panel of
the temperature controller. Select the correct input for display of the sensor (TC-K,
see Section A.2).
2) Fill the µ-Slide or µ-Dish with medium and place the sensor as close as possible to
the later position of the cells. Use the prepared calibration set, which consists of a µ-
Dish or a µ-Slide with a perforated lid, as shown in the Figure .
3) Set all parameters to create conditions identical to those of the actual experiments,
such as room temperature, air conditioning, airflow, illumination, microscope settings,
etc.
4) Set the temperature of heated plate to the desired cell temperature and set the
temperature of the lid several degrees higher (e.g., plate 37 °C, lid 42 °C). You can do
this directly with the control buttons on the controller, or with the TempControl
software.
5) Let the system equilibrate for 30 minutes.
6) Go to the ‘Calibration’ mode of the controller (see Section A.4.4) and execute the
‘Calibrate Now’ function for the channel called ‘Plate’, because the heated plate is the
main heat source for the cells.
The displayed temperatures are valid for the heated
plate and the heated lid only. The temperature near
the cells might be different (lower in most cases)!
ibidi Heating System 1, Instruction Manual
© ibidi GmbH 2012, version 2.1, revision 1, 2012-10-09, Dr. Miro Kroutvar, Dr. Zeno Guttenberg 25
7) If you use the TempControl software, you can calculate the difference between the
measured cell temperature with the external sensor and the displayed temperature.
This value can then typed in as the ‘Offset’ in the corresponding window of the
channel configuration. This method is described in more detail in Section 5.3.1.
8) Check the temperature of the system from time to time.
Figure 8: Sensor Placement for Temperature Calibration
4.2 Influence of Surrounding Temperature and Airflow
The surrounding, ambient temperature affects the temperature inside the microscopy
chambers. Devices, such as computers and camera controllers, can significantly heat up
small rooms. In this case, we recommend equilibrating the room temperature to the typical
experimental conditions at least 2-3 hours before calibrating.
Keep in mind that the temperature sensor can also measure room temperatures.
You will be able to calibrate any other connected, heated
device in the same way.
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26 ©ibidi GmbH 2012, version 2.1, revision 1, 2012-12-03, Dr. Miro Kroutvar, Dr. Zeno Guttenberg
The airflow can enhance the effect of temperature changes in the vicinity of the incubation
chamber. In a case where the airflow (e.g., air conditioning, etc.) cannot be stopped, we
recommend protecting the microscope as much as possible from any strong airflow. Please
contact us for support.
ibidi Heating System 1, Instruction Manual
© ibidi GmbH 2012, version 2.1, revision 1, 2012-10-09, Dr. Miro Kroutvar, Dr. Zeno Guttenberg 27
5 TempControl Software
The ibidi Temperature Controller has an USB interface for computer control. The
TempControl software is the tool that was developed for this remote control, and comes
with the controller.
5.1 Software Overview
In the main view window of the TempControl software, you can set the temperature for
each individual channel, plus you can view the measured temperature, and also the
temperature course. Additionally, you can find indicators for the channel status and the
communication between the controller and the computer.
From this view, you can access further sub-menus. By
activating the ‘Monitoring’ button, you get a minimized view of
the measured temperatures for each channel, as shown in
Figure . You can use this feature when your desktop space is
limited.
By clicking inside the temperature graph, you can expand the
graphical presentation of the temperature course, as seen in
Figure .
Figure 9: Main View of TempControl Window
Graphical temperature progress
Click to expand
Activate minimized
temperature monitor
Channel status
Channel ON/OFF switch
Measured temperature
Set temperature
Figure 10: Minimized View
of the Temperature
Monitoring Window
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28 ©ibidi GmbH 2012, version 2.1, revision 1, 2012-12-03, Dr. Miro Kroutvar, Dr. Zeno Guttenberg
The ‘Logging Menu’ button brings you to a menu where you can start logging in the
temperature course. The ‘Advanced’ button leads you to a menu where the channel
parameters can be viewed and modified.
Figure 11: Graphical Presentation of the Temperature Course
ibidi Heating System 1, Instruction Manual
© ibidi GmbH 2012, version 2.1, revision 1, 2012-10-09, Dr. Miro Kroutvar, Dr. Zeno Guttenberg 29
5.2 Logging Menu
If you choose ‘Start Logging’, you have to select a file name under which the data should be
stored. This data can be viewed in a normal text viewer or it can be loaded using the ‘Open’
button.
There are two further options for the data logging. If you select ‘Advanced Logging’, you can
save disk space, because only the last 30 minutes will be stored. If you select the ‘Append
Values’ box, then, in addition to the last 30 minutes, all data after an incident is recorded, as
in the case of a temperature alert.
5.3 Advanced Menu
The ‘Advanced’ menu allows you to configure the individual channel parameters, starting
from the PID algorithm coefficients over the input limits, to the alarm settings. There, you
can also label the channels according to the heating device. You will find that Channel 1 is
labeled ‘Lid’ and Channel 2 is labeled ‘Plate’.
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30 ©ibidi GmbH 2012, version 2.1, revision 1, 2012-12-03, Dr. Miro Kroutvar, Dr. Zeno Guttenberg
An interesting option for many users is the possibility of defining a temperature offset. This
is a simple way to calibrate the controller.
5.3.1 Calibration Routine Using TempControl
Using the external temperature sensor, measure the temperature at the cell position, as is
shown in Section 4.1. Calculate the difference between the temperature measured by the
integrated sensor of the heating device and that of the external sensor. Use this value as the
offset. If, after changing the offset the temperature still deviates, repeat this step.
Figure 12: Advanced Menu for Control Channel Configuration
ibidi Heating System 1, Instruction Manual
© ibidi GmbH 2012, version 2.1, revision 1, 2012-10-09, Dr. Miro Kroutvar, Dr. Zeno Guttenberg 31
6 Troubleshooting
6.1 Focus Not Stable
Focus drift is a disturbing effect, especially during time-lapse experiments. Focus stability is
mainly influenced by mechanical changes and temperature variations. Follow these
recommendations to keep your cells in focus:
• Switch on all components (heating, gas incubation, computer, other equipment) at
least 60 minutes before starting the experiment.
• After you put the µ-Slide onto the microscope, wait 20 minutes before starting a
time-lapse experiment to achieve temperature and immersion oil equilibration. (*)
• Keep the room temperature as stable as possible. Air conditioning should either be
working continuously or switched off.
• Do not change the temperature during the experiments. Avoid door/window
openings, as this could rapidly change the temperature.
• Eliminate all sources of mechanical vibrations. Use a damped table for your
microscope.
(*) In case the experiment needs to be started immediately, either after placing the slide on
the microscope, or after closing the lid, we recommend controlling the focus for 20 minutes.
In the first minutes after starting the experiment, temperature equilibration might influence
the focus/z-position of the cells.
6.2 Evaporation Too High
Depending on the incubating conditions, small volumes might evaporate quickly, especially
during long-term experiments. We suggest using Parafilm (Pechiney Plastic Packaging
Company) or silicone oil AR 200 (Fluka, Sigma-Aldrich) to decrease evaporation. If you have
an actively controlled humidifying device, increase the set value of relative humidity.
6.2.1 Parafilm Procedure
Small stripes of Parafilm fitted onto the reservoirs of the slides are very effective at
preventing evaporation effects. Fill the slide, as recommended, then adapt the Parafilm until
it fits tightly.
6.2.2 Silicone Oil Procedure (ibidi’s Anti-Evaporation Oil)
Covering the medium with sterile silicone oil prevents all evaporation effects and is
compatible with cell culture. Please don’t use mineral oil, as this would be harmful to the
ibidi µ-Slides.
Equilibrate oil and medium inside the incubator overnight. This step helps avoid the
formation of air bubbles, and pre-warms the solutions to 37°C. Afterwards, fill your slide
with cells and medium. Cover the medium’s surface with an appropriate amount of silicone
oil. Don’t drip the oil directly onto the surface, but let it run down the edges by pressing the
ibidi Heating System 1, Instruction Manual
32 ©ibidi GmbH 2012, version 2.1, revision 1, 2012-12-03, Dr. Miro Kroutvar, Dr. Zeno Guttenberg
pipette tip directly on the upper side of the reservoir. For example, when using µ-Slide
VI 0.4, fill each reservoir with 30 µl cell-free medium and 30 µl silicone oil.
Please contact us at [email protected] for further troubleshooting help.
ibidi Heating System 1, Instruction Manual
© ibidi GmbH 2012, version 2.1, revision 1, 2012-10-09, Dr. Miro Kroutvar, Dr. Zeno Guttenberg 33
7 WEEE/RoHS Compliance Statement
EU Directives WEEE and RoHS
To Our Valued Customers:
ibidi GmbH is committed to being a good corporate citizen. As part of that commitment, we
strive to maintain an environmentally conscious manufacturing operation. The European
Union (EU) has enacted two directives, the first on product recycling (Waste Electrical and
Electronic Equipment, WEEE) and the second on limiting the use of certain substances
(Restriction on the use of Hazardous Substances, RoHS).
Two Categories of products covered by the WEEE Directive are currently exempt from the
RoHS Directive – Category 8, medical devices (with the exception of implanted or infected
products) and Category 9, monitoring and control instruments.
All of our products fall into either Category 8 or 9, and are currently exempt from the RoHS
Directive. We will, however, continue to monitor the application of the RoHS Directive in
relation to our products and will comply with any changes, as they apply.
Recycling is offered for our products that fall within the scope of the WEEE Directive. These
specific products, available for sale after August 13, 2005, will be identified with a “wheelie
bin” symbol.
When you see this symbol, do not dispose of this product with Municipal Waste. Special
Collection/Disposal is required.
Please contact us for feedback or further information.
ibidi GmbH
Am Klopferspitz 19
82152 Planegg-Martinsried
Germany
www.ibidi.com
Tel.: +49 89 520 4617 0
Fax: +49 89 520 4617 59
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34 ©ibidi GmbH 2012, version 2.1, revision 1, 2012-12-03, Dr. Miro Kroutvar, Dr. Zeno Guttenberg
Ibidi Heating System 1, Instruction Manual
© ibidi GmbH 2012, version 2.1, revision 1, 2012-12-03, Dr. Miro Kroutvar, Dr. Zeno Guttenberg I
Appendix
A Menu Navigation
Most of the functions and parameters of the ibidi Temperature Controller can be accessed
with the control buttons on the front panel. The LC display shows the changes. However,
the display is limited to 20 characters per line and four lines. Therefore, some of the fields
are out of the initial view. Nevertheless, they are reachable by using the ’Up / Right’ and
’Down / Left’ arrow buttons. The view shifts accordingly.
By default, there are four channels displayed, but only three of them fit into the viewing
frame. Therefore, to reach the last channel you need to push the ’Up / Right’ arrow button
once more, after you have reached the Channel 3. Then, Channel 4 is displayed and Channel
1 vanishes. By using the ’Down / Left’ arrow button, you will reach Channel 1 again.
A.1 Setting the Temperature
By pressing the control buttons, the cursor on the display will change position. When you
are at the parameter you want to change, you need to select the field with the round ‘Select’
button and change the value using the ‘Up / Right’ and ‘Down / Left’ arrow buttons. To
confirm the change, you need to press the ‘Select’ button again.
Channel names
Measuredte
mperature
Set
temperature
Cursor
Externaltemp
eraturesenso
r
Setup
functions
Run /
Stopbutton
Indicatorfor
additional
view Up / Right
Select / Confirm
Down / Left
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II ©ibidi GmbH 2012, version 2.1, revision 1, 2012-12-03, Dr. Miro Kroutvar, Dr. Zeno Guttenberg
A.2 External Temperature Sensor
The ibidi Temperature Controller has plugs for two different temperature sensors:
(a) Type-K plug for a thermo-couple sensor
(b) Plug for a 4-terminal sensing of a Pt100 resistor
You can choose which sensor you want to have displayed and use it as the reference
sensor:
• Thermo-couple: ‘TC-K’
• Pt100 sensor: Pt100
• No selection: ‘NoSel’
Ch1 Ch2 Ch3 Ch4I 42.1 36.9 37.3 OffS 42.0 37.0 37.3 Off
E [25.3] Run Setup
Ch1 Ch2 Ch3 Ch4I 42.1 36.9 37.3 OffS 42.0 37.0 37.3 Off
E <Pt100>Run Setup
Ch1 Ch2 Ch3 Ch4I 42.1 36.9 37.3 OffS 42.0 37.0 37.3 Off
E <TC-K>Run Setup
Ch1 Ch2 Ch3 Ch4I 42.1 36.9 37.3 OffS 42.0 37.0 37.3 Off
E <NoSel>Run Setup
A.3 Run / Stop Button
With the ‘Run / Stop’ button, you can set all control channels to standby.
Ch1 Ch2 Ch3 Ch4I 42.1 36.9 37.3 OffS [42.0] 37.0 37.3 OffE 25.3 Run Setup
Ch1 Ch2 Ch3 Ch4I 42.1 36.9 37.3 OffS 42.0 [37.0] 37.3 OffE 25.3 Run Setup
Ch1 Ch2 Ch3 Ch4I 42.1 36.9 37.3 OffS 42.0 37.0 [37.3] OffE 25.3 Run Setup
…
Ch1 Ch2 Ch3 Ch4I 42.1 36.9 37.3 OffS 42.0 <37.0> 37.3 OffE 25.3 Run Setup
Ch1 Ch2 Ch3 Ch4I 42.1 36.9 37.3 OffS 42.0 <37.1>37.3 OffE 25.3 Run Setup
Ch1 Ch2 Ch3 Ch4I 42.1 36.9 37.3 OffS 42.0 <37.2>37.3 OffE 25.3 Run Setup
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A.4 Setup Button
Using the ‘Setup’ button, you can reach further functions, such as the channel output
‘Mode’, the ‘Alarm’ function, the controller ‘Preferences’, the ‘Calibration’ function, and you
can view the ’Info’ (information) about the controller.
A.4.1 Mode Function
In this field, you can switch the channels individually ‘On’ and ‘Off’. For that, you have to
select the corresponding channel number and change the ‘Mode’ from ‘On’ to ‘Off’.
Ch1 Ch2 Ch3 Ch4I 42.1 36.9 37.3 Off
S 42.0 37.0 37.3 Off
E 25.3 [Run] Setup
Ch1 Ch2 Ch3 Ch4
I 42.1 36.9 37.3 Off
S 42.0 37.0 37.3 OffE 25.3 <Run> Setup
Ch1 Ch2 Ch3 Ch4
I 42.1 36.9 37.3 Off
S 42.0 37.0 37.3 OffE 25.3 <Stop> Setup
Setup <Mode>
Channel = 1Mode = On
Return
Setup <Alarms>
Channel = 1High = +40.0 °CLow = +25.5 °C
Return
Setup <Preferences>
Backlight = 65Contrast = 25
Return
Setup <Calibration>Channel 1 Corr +0.0
Calibrate nowMeas +42.1 Ref +41.5
Return
Setup <Info>TempCoSN: xxxxFirmware 1.01
Return
Setup <Return>
…
…
Ch1 Ch2 Ch3 Ch4I 42.1 36.9 37.3 OffS 42.0 37.0 37.3 OffE 25.3 Run [Setup]
Ch1 Ch2 Ch3 Ch4I 42.1 36.9 37.3 OffS 42.0 37.0 37.3 OffE 25.3 Run [Setup]
Setup <Mode>
Channel = 1Mode = On
Return
Setup <Alarms>
Channel = 1High = +40.0 °CLow = +25.5 °C
Return
Setup <Mode>
Channel = 1Mode = On
Return
Setup <Alarms>
Channel = 1High = +40.0 °CLow = +25.5 °C
Return
Setup <Alarms>
Channel = 1High = +40.0 °CLow = +25.5 °C
Return
Setup <Mode>
Channel = 1Mode = On
Return
Setup <Alarms>
Channel = 1High = +40.0 °CLow = +25.5 °C
Return
Setup <Mode>
Channel = 1Mode = On
Return
Setup <Alarms>
Channel = 1High = +40.0 °CLow = +25.5 °C
Return
Setup <Info>TempCo
SN: ibiTC-xxxFirmware 0.35E5
Return
Setup <Return>
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IV ©ibidi GmbH 2012, version 2.1, revision 1, 2012-12-03, Dr. Miro Kroutvar, Dr. Zeno Guttenberg
Setup Mode
Channel = [1]Mode = On
Return
Setup Mode
Channel = 1Mode = [On]
Return
Setup Mode
Channel = 1Mode = On
[Return]
Setup ModeChannel = <1>
Mode = On
Return
Setup ModeChannel = 1
Mode = <On>
Return
Setup ModeChannel = 1
Mode = <Off>
Return
Setup <Mode>Channel = 1
Mode = OnReturn
A.4.2 Alarm Function
If you activate the ‘Alarm’ function, you will get a visual (�’High’ or ‘Low’ message on the
LCD) and optionally acoustical signal (� Beeper, please contact ibidi). The alarm goes off
when the measured temperature exceeds the upper or lower thresholds of the alarm limits.
The limits are individually programmable. You can set the thresholds as absolute or relative
temperatures using the TempControl software.
Setup AlarmsChannel = [1]
High = +40.0 °CLow = +25.5 °C
Return
Setup AlarmsChannel = 1
High = [+40.0] °CLow = +25.5 °C
Return
Setup AlarmsChannel = 1
High = +40.0 °CLow = [+25.5] °C
Return
Setup AlarmsChannel = 1
High = +40.0 °CLow = +25.5 °C
[Return]
Setup AlarmsChannel = <1>High = +40.0 °CLow = +25.5 °C
Return
Setup AlarmsChannel = 1High = <+40.0>°CLow = +25.5 °C
Return
Setup AlarmsChannel = 1High = <+40.1>°CLow = +25.5 °C
Return
Setup AlarmsChannel = 1High = +40.0 °CLow = <+25.5>°C
Return
Setup <Alarms>Channel = 1High = +40.0 °CLow = +25.5 °C
Return
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© ibidi GmbH 2012, version 2.1, revision 1, 2012-12-03, Dr. Miro Kroutvar, Dr. Zeno Guttenberg V
A.4.3 Preferences
Setup <Preferences>
Backlight = 65
Contrast = 25Return
Setup PreferencesBacklight = [65]
Contrast = 25
Return
Setup PreferencesBacklight = 65
Contrast = [25]
Return
Setup PreferencesBacklight = 65
Contrast = 25
[Return]
Setup Preferences
Backlight = 65
Contrast = <25>Return
Setup Preferences
Backlight = 65
Contrast = <26> Return
Setup PreferencesBacklight = <65>
Contrast = 25
Return
Setup PreferencesBacklight = <66>
Contrast = 25
Return
The display settings of the controller can be changed under ‘Preferences’. You can modify
the brightness of the display (‘Backlight’) and the contrast.
A.4.4 Calibration
The ibidi Temperature Controller has a built-in function for calibrating the controlled channels.
To do this, there are two plugs for external temperature sensors on the rear panel of the
controller. In this menu, you will find the channel number, the currently measured
temperature ‘Meas’, the sensed temperature ‘Ref’ of the external sensor, and the currently
used temperature offset ‘Corr’. You can change the reference sensor, as described in
Section A.2.
ibidi Heating System 1, Instruction Manual
VI ©ibidi GmbH 2012, version 2.1, revision 1, 2012-12-03, Dr. Miro Kroutvar, Dr. Zeno Guttenberg
Setup <Calibration>Channel 1 Corr +0.0Calibrate now
Meas +42.1 Ref +41.5Return
Setup Calibration
Channel [1]Corr +0.0Calibrate nowMeas +42.1 Ref +41.5
Return
Setup CalibrationChannel 1 Corr +0.0
Calibrate [now]Meas +42.1 Ref +41.5
Return
Setup Calibration
Channel 1 Corr +0.0Calibrate nowMeas +42.1 Ref +41.5
[Return]
Setup CalibrationChannel <1> Corr +0.0Calibrate now
Meas +42.1 Ref +41.5Return
Setup CalibrationChannel<2>Corr +0.0Calibrate now
Meas +42.1 Ref +41.5Return
Setup CalibrationChannel 1 Corr -0.6Calibrate now
Meas +41.5 Ref +41.5Return
automatic
Once you have activated the calibration ‘Calibrate Now’, the controller will calculate an offset
from the temperature difference between the control channel and the external sensor. This
offset will replace the former value of ‘Corr’.
A.4.5 Info
Setup <Info>TempCoSN: ibiTC-xxxFirmware 0.31E5
Return
In this menu, you can find the serial number of the device, as well as the hardware and
firmware versions.