International Conference on
Biodiversity
Abs Soc Indon Biodiv vol. 6 | no. 3 | pp. 67-116 | November 2019
ISSN: 2407-8069
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TIME SCHEDULE
International Conference on Biodiversity
Society for Indonesian Biodiversity (SIB)
Surakarta, Indonesia, 11-12 November 2019
TIME ACTIVITIES PERSON IN CHARGE SITE
November 11, 2019
07.30-08.30 Registration Committee Lobby
08.30-08.40 Opening ceremony Chairman of the SIB (on behalf) R1
08.40-09.00 Photo session and coffee break Committee R1,
Closing ceremony & other explanation
Chairman of the Board of Assessors
Chairman of the Committee
R1
November 12, 2019
07.30- ... City tour [optional] Committee Lobby
Lobby
09.00-10.30 Panel I Moderator R1
Prof. Jean W.H. Yong
Prof. Takaomi Arai
10.30-12.00 Panel II Moderator R1
Dr. Sunardi Dr. Hamim
12.00-13.00 Rest, prayer, lunch & Poster session Committee Lobby
13.00-14.00 Parallel presentation I
Group 1: AO-01 to AO-07 Moderator R1
Group 2: AO-08 to AO-14 Moderator R2
Group 3: AO-15 to AO-21 Moderator R3
Group 4: AO-22 to BO-06 Moderator R4
Group 5: BO-07 to BO-13 Moderator R5
14.00-15.00 Parallel presentation II
Group 6: BO-14 to BO-20 Moderator R1
Group 7: BO-21 to BO-27 Moderator R2
Group 8: BO-28 to BO-34 Moderator R3
Group 9: BO-35 to CO-07 Moderator R4
Group 10: CO-08 to DO-05 Moderator R5
15.00-15.15 Coffee break, prayer Committee Lobby
15.15-16.15 Parallel presentation III
Group 11: DO-06 to DO-11 Moderator R1
Group 12: DO-12 to EO-02 Moderator R2
Group 13: EO-03 to EO-08 Moderator R3
Group 14: EO-09 to EO-14 Moderator R4
Group 15: EO-15 to EO-20 Moderator R5
16.15-16.30 Announcement of the Best Presenters
iv
RELATED ACTIVITIES
Workshop on Biodiversity Conservation & Bioprospecting
TIME ACTIVITIES PERSON IN CHARGE SITE
November 11, 2019
07.30-08.30 Registration Committee Lobby
08.30-08.40 Opening ceremony Chairman of the SIB (on behalf) R1
08.40-09.00 Photo session and coffee break Committee R1,
area, dryland area, and other
developed areas
Dr. Ruhyat Partasasmita
12.00-13.00 Rest, prayer, lunch Committee Lobby
13.00-15.00 Session II Moderator R6
Biodiversity and ecotourims in
mangrove ecosystem
Prof. Jean W.H. Yoon
15.00-15.15 Coffee break, prayer Committee Lobby
15.15-16.15 Session III Moderator
How to write good papers for reputable
international journals
R6
Prof. Dwi Astiani
Lobby
09.00-09.30 Prelude Head of Enviro Sci Program UNS (on behalf) 09.00-12.00 Session I Moderator R6
Utilization of biodiversity in urban
TABLE OF CONTENTS
International Conference on Biodiversity
Society for Indonesian Biodiversity (SIB)
Surakarta, Indonesia, 11-12 November 2019
CODE TITLE AUTHOR(S) PAGES
Genetic diversity
AO-01 Morphometric diversity and genetic relationship analyze to determine potential indigenous buffaloes of Banten,
Indonesia
Dewi Murni, Umie Lestari, Sri
Endah Indriwati, Achmad Efendi,
Nani Maryani, Mohamad Amin
67
AO-02 New DNA barcode primer assembly for Indonesian Bamboo
Annisa, Rini Hafzari, Tia
Setiawati, Joko Kusmoro
67
AO-03 Genetic diversity of sago palm (Metroxylon sagu) accessions based on plastid cpDNA matK gene as DNA
barcoding
Barahima Abbas, Ihwan Tjolli,
Munarti
68
AO-04 Morphological diversity and the cultivation practices of Laportea decumana as a medicinal plant in the highland
area of Puncak District, Papua Province, Indonesia
Marpen Y. Tabuni, Saraswati Prabawardani, Nouke L.
Mawikere
68
AO-05 Spatial modeling for kedondong (Spondias dulcis) cultivation in Central Part of Sumatra, Indonesia
Ibna Hayati, Alex Hartana, Nina
Ratna Djuita
68
AO-06 Shade stress in various growth phases and its effect on agronomic characters and chlorophyll content
of peanut genotypes
A. Farid Hemon, Sumarjan,
Hanafi Abdurachman
69
AO-07 Erythrina fusca Lour. on Mount Merapi, Central Java, Indonesia
ILG Nurtjahjaningsih, AYPBC
Widyatmoko, Purnamila
Sulistyawati, Bangun Bramantya,
Putu Dhian Budhami, Lukman
Hakim, Yayan Hadiyan, Liliek
Haryjanto, Sugeng Pudjiono, Dedi
Setiadi, Y. Wibisono
69
vi
AO-08 Genetic diversity of tesek (Dodonaea viscosa Jacq.) from Mount Merapi based on Random Amplified Polymorphic
DNA markers
69
AO-09 Genetic similarity of 35 cassava (Manihot esculenta) clones by RAPD
Rani Agustina Wulandari, Qonita
Luthfi Nur Hanifah, Christin
Triagustina, Aisyah Fitri Rohani,
Patrisia Citra Saraswati, Tri
Harjaka
70
AO-10 Bacillus spp. strain from Indonesian honey and other-bee associated products as probiotic candidates
Stella Magdalena, Gracia
Nathalie, Ivana Purnawidjaja,
Yogiara
70
AO-11 Identification and pathogenicity of Colletotrichum spp. associated with chili anthracnose in Yogyakarta,
Indonesia
Dhika Sri Anggrahini, Arif
Wibowo, Siti Subandiyah
71
AO-12 Realized genetic gain on oil productivity resulted from breeding program of cajuput and its impact on farmer’s
income
Sumardi, Noor Khomsah
Kartikawati, Prastyono, Arif
Nirsatmanto, Anto Rimbawanto
71
AO-13 Morphological and molecular characterization of Kryptopterus bicirrhis from Riau, Indonesia
Roza Elvyra, Meyla Suhendra,
Widya Hidayana
71
AO-14 Variation of prolactin gene in the Indonesian Frisian-Holstein cattle
Paskah Partogi Agung, Ari
Sulistyo Wulandari, Sharon
Hillary
71
AO-15 Pollen fertility of F1 crossing local rice with drought tolerant rice
Aditya Wahyudhi, Panjisakti
Basunanda
72
AO-16 Pod yield and haulm weight peanut cultivars grown in dry lands with dry climate
Agustina Asri Rahmianna, Andy
Wijanarko, Yuliantoro Baliadi
72
AO-17 Antixenosis of soybean promising lines and resistance levels to pod borer, Etiella zinckenella
Marida Santi Yudha Ika Bayu,
Gatut Wahyu Anggoro Susanto,
Yusmani Prayogo
72
AO-18 Physical and chemical properties of sixty peanut germplasm
Erliana Ginting, Novita
Nugrahaeni
73
AO-19 The phenology and morphology characteristic of nine shallot cultivars from TSS in the low land area,
Yogyakarta, Indonesia
Retno Pangestuti, Endang
Sulistyanigsih, Budiastuti
Kurniasih, Rudi Hari Murti,
Stephen Harper, Siti Subandiyah
73
AO-20 Evaluation of flowering of cassava clones: the effect of genotypes, altitude, paclobutrazol, and grafting with
rootstock Manihot glaziovii
Setyo Dwi Utomo, Erwin Yuliadi,
Akari Edy, Agustiansyah, Kresna
Shifa Usodri, Fajar Danu Aslami,
Mustika Adzania Lestari
74
Purnamila Sulistyawati, AYPBC
Widyatmoko, ILG Nurtjahjaningsih, Liliek
Haryjanto, Yayan Hadiyan,
Lukman Hakim, Dedi Setiadi,
Sugeng Pudjiono, Toni Herawan,
Yohannes Wibisono, Bangun
Baramantya, Dhani Suryawan
vii
AO-21 Evaluation of crossing local rice with drought tolerance rice by molecular markers SSR
Aditya Wahyudhi, Panjisakti
Basunanda
74
AO-22 Identification of leptin gene in Belgian blue cross breed cattle
Laksa Ersa Anugratama, Tety
Hartatik
74
AP-01 Genetic diversity of low-risk Stelechocarpus burahol from Magelang and Purworejo (Central Java, Indonesia)
revealed by ISSRs
Tri Suwarni Wahyudiningsih,
Esna Dilli Novianto, Erry
Purnomo, Dian Sartika
75
AP-02 Seed coating plus biological agents to increase of rice growth for blast disease in seedling
Tantri Palupi, Fadjar Riyanto 75
AP-03 Analysis of lactate dehydrogenase gene expression as an adaptation strategy for jambal catfish to stress the aquatic
environment
Sularto, Lies Emmawati Hadie,
Huriah Marnis, Jadmiko
Darmawan, R.R. Sri Puji Sinarni
Dewi
75
AP-04 Diversity of Central Sulawesi local mangosteen (Garcinia mangostana) genotypes in correlation with
resistance to drought stress
Enny Adelina, Nuraeni, Yohanis
Tambing
76
AP-05 Genetic variation of agarwood producing tree (Gyrinops versteegii) from Pongkor, Manggarai District, Flores
Island, Indonesia using ISSR molecular markers
Alfi Fauzan Irsyad, Ridesti
Rindyastuti, Titut Yulistyarini,
Agung Sri Darmayanti, Budi
Setiadi Daryono
76
AP-06 Resistance varieties and pattern of disease progress of rust (Pucciana horiana) in chrysanthemum
Yayuk Aneka Bety, Retno
Pangestuti
76
AP-07 Physico-chemical characteristics of elephant foot yam (Amorphophallus campanullatus) germplasm
Joko Susilo Utomo, Rahmi
Yulifianti, Erliana Ginting
77
AP-08 Morphological characterization of Lombok upland rice, West Nusa Tenggara, Indonesia
Baiq Sri Hartina, Rani Agustina
Wulandari, Panjisakti Basunanda
77
AP-09 Morphological and genetic changes of Spathoglottis plicata seedlings resulted from X-Ray irradiated seeds
Suyitno Aloysius, Aziz
Purwantoro, Kumala Dewi,
Endang Semiarti
77
Diversity of Species
78
BO-01 Fish hosts of the freshwater mussel Unio foucauldianus Pallary, 1936
Hassan Benaissa, Amílcar
Teixeira, Manuel Lopes-Lima,
Ronaldo Sousa, Simone Varandas, Hanane Rassam,
Mohamed Ghamizi
BO-02 Diversity of endophyte Colletotrichum spp. from quina plant (Cinchona calisaya) base on its and actin rDNA
sequence
Nani Radiastuti, Dwi N.
Susilowati, Zhafira Amila Haqqa
78
BO-03 Linking of forest genetic resources, breeding and industry: A case study in developing new variety of
Acacia hybrid (Acacia mangium × Acacia
auriculiformis) for pulp and paper industry
Sri Sunarti, Arif Nirsatmanto 78
viii
BO-04 Biodiversity wild plants, indigenous, edible and nutrient substances: Case study in Suka Maju and Tamao
Villages, Kapuas Hulu, West Kalimantan, Indonesia
Sulvi Purwayantie, Uray Edi
Suryadi
79
BO-05 Diversity of bird species in the coffee agroforestry landscape: Case study in Pangalengan District, Bandung
District, West Java, Indonesia
Susanti Withaningsih, Parikesit,
Rizka Fatharani Alham
79
BO-06 Re-vegetation practices, invasive species and forestry industry: a compromise among biological factors toward
eco-friendly Acacia plantation for local biodiversity
Arif Nirsatmanto 80
BO-07 Ecological indices on biodiversity of lichen as bioindicator of ambient air
Efri Roziaty, Sutarno, Suntoro
Suntoro, Sugiyarto
80
BO-08 Rice landraces of Tana Toraja, South Sulawesi, Indonesia: A study on digital conservation
Ikma C. Ranteallo, Azwar H.
Nasution, Meredian Alam, Lala
M. Kolopaking, Djuara P. Lubis,
Ervizal A.M. Zuhud
80
BO-09 Land use change on insect visitor diversity in coffee orchards in East Java, Indonesia
Hafsah, Dini Siti Anggraeni,
Iriawati, Tati Suryati Syamsudin
81
BO-10 Associated biodiversity in the area of habitat quality improvement in Taman Buru Gunung Masigit Kareumbi,
West Java, Indonesia
Parikesit, Susanti Withaningsih,
Ratna Wingit
81
BO-11 Analysis of local-varieties brown rice to salinity tolerance
Mawaddah Putri, Arisma Siregar,
Chairani Hanum, Lutfi A.M.
Siregar
81
BO-12 Call characteristic of Philautus sp. (Amphibia: Anura) from Gunung Ungaran, Central Java, Indonesia: a
preliminary study
Tundiyati, Margareta
Rahayuningsih
82
BO-13 The diversity of fish species in the Kaligarang River, Semarang City, Indonesia
Ela Puji Aprilliyani, Margareta
Rahayuningsih
82
BO-14 Performances of legume and non-legume trees under controlled dry treatment and dry Karst Field Area
Rina Laksmi Hendrati, Nur
Hidayati, Ari Fiani
82
BO-15 How far arbuscular mycorrhiza fungi and rhizobium enhance the growth of trembesi (Samanea saman) on
gold mine tailings?
Luluk Setyaningsih, Firli Azhar
Dikdayatama, Arum Sekar
Wulandari
82
BO-16 Habitat profile and distribution pattern of Dendrobium jacobsonii orchid in Cetho Temple Hiking Trail, Mount
Lawu, Central Java, Indonesia
Gilang Dwi Nugroho, Suratman,
Sugiyarto
83
BO-17 Population of arbuscular mycorrhizal fungi in oil palm rhizosphere on various of peat water tables
Hanna Artuti Ekamawanti,
Burhanuddin, Dwi Astiani, Wiwik
Ekyastuti, Emi Roslinda
83
BO-18 Biodiversity of endophytic fungi from Vernonia amygdalina, phenolic and flavonoids content and their
bioactivities
Praptiwi, Ahmad Fathoni, M.
Ilyas
83
BO-19 The diversity of Araceae in Siau, Sangihe and Talaud Archipelago, North Sulawesi, Indonesia
Ni Putu Sri Asih, Agung
Kurniawan, Ina Erlinawati
84
ix
BO-20 Innovation of biological control technology for mungbean pests and diseases
Yusmani Prayogo, Marida Santi
Yudha Ika Bayu, Gatut Wahyu
Anggoro Susanto
84
BO-21 Population of rhizosphere bacteria in oil palm plantations in peatlands with the management of water table levels
Wiwik Ekyastuti, Dwi Astiani,
Burhanuddin, Hanna Artuti
Ekamawanti, Emi Roslinda
84
BO-22 Spatial and temporal phytoplankton diversity in developing urban coastal area: Study case in Tuban,
Indonesia
Edwin Setiawan, Nova Maulidina
Ashuri, Alfian Amrullah, Fa’izah
Nur Shabrina, Marita Ika
Joesidawati, Suwarsih, Dewi
Hidayati
85
BO-23 Potency, natural distribution and habitat condition of six endangered high-value indigenous wood species of
Sumatra
Eka Novriyanti, Ahmad Juneadi,
Dodi Frianto
85
BO-24 Molecular identification of amylase-producing thermophilic bacteria isolated from Bukit Gadang Hot
Spring, Solok, West Sumatra, Indonesia
Aulia Ardhi, Arina Sidaruk,
Nabella Suraya, Nova Wahyu
Pratiwi, Usman Pato, Saryono,
85
BO-25 Screening of cellulase-producing thermophilic fungi isolated from hot springs in West Sumatra
Nova Wahyu Pratiwi, Nabella Suraya, Riryn Novianti, Aulia
Ardhi, Saryono,
86
BO-26 New genus of endosymbiont bacteria in Rhizopus microsporus
Dewi Peti Virgianti, Desi Natalia,
I Nyoman Pugeg Aryantha
86
BO-27 Dry season Bryophytes in Cangar Hotspring-Taman Hutan Raya Raden Soeryo, East Java, Indonesia
Iska Desmawati, Angga Gian, Rizki Widyaningsih, Zulfann
Febriawan, Saniah Kusnatur, Ory
Kurnia, Alwi Muhammad, Dimas
Raihan
86
BO-28 Botanical inventory and ecology of the Genus Cyathea in Submontane of the Southern Slopes of Mt. Salak,
western Java, Indonesia
Titien Ngatinem Praptosuwiryo 87
BO-29 Effect of thermo-tolerant lignocellulolytic fungi as substrate bio activator on oyster mushroom production
(Pleurotus ostreatus)
Atria Martina, Nova Wahyu
Pratiwi, Rodesia Mustika Roza,
Mira, Febriani, Imelda Wardani
87
BO-30 The herbivore assemblage, herbivory and leaf chemistry of the mangrove Rhizophora apiculata in two mangrove
forests in East Java, Indonesia
Indah Trisnawati, Mukhammad
Muryono, Iska Desmawati
87
BO-31 Variation of body size and shape of Baduy Muslim, Indonesia
Eneng Nunuz Rohmatullayaly,
Ruhyat Partasasmita, Johan
Iskandar
88
BO-32 Production of Cinchona alkaloid from endophytic fungus (Colletotrichum spp.) of Cinchona plant (Cinchona
calisaya)
Nani Radiastuti, Wiwid Wildatus
Sholihah, Siti Nurbayti
88
BO-33 Biochemical test on seed rice of three varieties Nalwida Rozen, Musliar Kasim, Aswaldi Anwar
88
BO-34 Meiotic abnormalities in male sterile Dendrobium hybrid Ari Pitoyo, Solichatun, Suratman 89
x
BO-35 Toxic effects of copper (Cu) exposure on survival and regeneration of freshwater planaria (Dugesia tigrina)
Sunardi, Sri Rahayu, Nadia
Istiqomah, Ade Pandi
89
BP-01 Early study of species richness of flora and fauna in Gondang Village, Kendal District, Central Java,
Indonesia
M. Rahayuningsih, M. Abdullah,
A. Marianti, A. Haning, A.
Handayani, Saniaturrohmah
89
BP-02 Diversity, density, and potential of mollusks in the intertidal zone of Pandawa beach, Kutuh Village,
Badung, Bali, Indonesia
Joko Wiryatno, Ketut Sundra 90
BP-03 Orchid exploration in Wan Abdul Rahman Great Forest Park, Lampung Province, Indonesia
Dwi Murti Puspitaningtyas 90
BP-04 Biopesticides based pest control on soybean in tidal swamp, Southern Kalimantan, Indonesia
Sri Wahyuni Indiati 90
BP-05 Diversity, conservation and potential of Euphorbiaceae in Bogor Botanical Gardens, Indonesia
Esti Munawaroh, Yuzammi 91
BP-06 Mosses diversity from Laewangi Wanggameti National Park, Sumba, East Nusa Tenggara, Indonesia
Florentina Indah Windadri, Dewi
Rosalina
91
BP-07 Leptospermum spp. (Myrtaceae) in Indonesia Siti Sunarti 91
BP-08 Pitcher morphology and microstructure of the pitcher surface in Nepenthes reinwardtiana (Nepenthaceae)
Tri Handayani, Hary
Wawangningrum
91
BP-09 Diversity of herpetofauna in Darajat, West Java, Indonesia
Teguh Husodo, Tatang S. Erawan,
Jirjiz Jauhan, Deri Achmad Fauzi,
Sya Sya Shanida, Indri
Wulandari, Irwananda Satria
Putra, Erri Noviar Megantara,
92
BP-10 The identification of flora reliefs in the Lalitavistara section of Borobudur temple, Central Java, Indonesia
Destario Metusala, Fauziah, Dewi
Ayu Lestari, Janis Damaiyani,
Shofiyatul Mas'udah, Hari
Setyawan
92
BP-11 Potential risk of various trees grown in the inner part of first zone to the preservation of Borobudur temple,
Central Java, Indonesia
Destario Metusala, Trimanto,
Laily Mukaromah, Dewi Ayu Lestari, Shofiyatul Mas'udah,
Fauziah, Mohamad Habibi
93
BP-12 Abundance and utilization of Chaetoceros sp. in Muara Gembong Waters, West Java, Indonesia
Adriani Sri Nastiti, Mujianto,
Krismono
93
BP-13 Orchid exploration in Central Java, Indonesia to enrich collections in Indrokilo Boyolali Botanic Gardens,
Indonesia
Ponco Yulianto, Titien Ngatinem
Praptosuwiryo, Dwi Murti
Puspitaningtyas
93
BP-14 Saving of critical Gembrong goat from extinction through crossbreeding
Bayu Andri Atmoko, I Gede
Suparta Budisatria, Sigit Bintara,
Dyah Maharani
93
xi
Diversity of Ecosystem
CO-01 Distribution of C-stocks in drainage areas on peatlands Sinta Haryati Silviana, Bambang Hero Saharjo, Sigit Sutikno
94
CO-02 Abundance model of bryophytes in Jumog Waterfall, Ngargoyoso, Karanganyar, Central Java, Indonesia
Efri Roziaty, Siti Naily Rohmah 94
CO-03 Response of geronggang (Cratoxylum arborescens) seedlings to pulp sludge compost addition in peat soil
Siti Wahyuningsih 95
CO-04 Vegetation analysis and economic benefits of damar (Agathis dammara) in Kawah Ratu Resort, Mount
Halimun Salak National Park, Indonesia
Yelin Adalina, Reni Sawitri 95
CO-05 Study of above-ground carbon stock at Kamojang Regions, West Java, Indonesia
Dwiki Danang Kuncoro, Teguh
Husodo, Joko Kusmoro, Randi
Hendrawan, Suroso, Femi Afriyanti
95
CO-06 Investigation of causal agent of garlic root rot disease in Central Java, Indonesia
Lutfi Arifin, Siwi Indarti, Arif
Wibowo
95
CO-07 Soil characteristics of an ex-burnt peatland in Kubu Raya District, West Kalimantan, Indonesia
Dwi Astiani, Darbin Simatupang 96
CO-08 Analysis of acid sulfate soil bacteria communities from two types of swampland in Kalimantan with the next
generation sequencing (NGS) approach
Dwi N. Susilowati, Erny Yuniarti,
Eva Moulia, Nani Radiastuti,
Yadi Suryadi, Surono
96
CO-09 Antifungal effect of endophytic bacteria isolated from Nusakambangan Island (Central Java, Indonesia) on
tomato (Lycopersicum esculentum) growth
Sri Yuwantiningsih, Rahayu
Widowati
96
CP-01 Population structure and floristic importance of Gyrinops versteegii in its natural habitat in Nggalak Forest, Flores,
Indonesia
Abban Putri Fiqa, Sugeng
Budiharta, Febrina Artauli
Siahaan, Ridesti Rindyastuti
97
CP-02 Distribution of Gyrinops versteegii in varying vegetation structures, soil properties, and microclimates in
Manggarai, Flores, East Nusa Tenggara, Indonesia
Titut Yulistyarini, Abban P. Fiqa,
Sugeng Budiharta, Ridesti
Rindyastuti
97
CP-03 Diversity and distribution patterns of tree ferns in the Mount Papandayan Conservation Area, West Java,
Indonesia
Suryana, Johan Iskandar,
Parikesit, Joko Kusmoro, Betty
Mayawatie, Budi Irawan, Ruhyat
Partasasmita
98
CP-04 Potential plant diversity in supporting food security in Rancakalong Village, Sumedang District, West Java,
Indonesia
Ruhyat Partasasmita, Eneng
Nunuz Romatullayali, Johan
Iskandar
98
CP-05 Rhizospheric microbe abundance, their functional diversity and biological activity of acid sulfate soil in
South Kalimantan, Indonesia
Erny Yuniarti, Surono, R. Cinta
Badia Ginting, D.N. Susilowati
98
CP-06 Diversity of macroscopic fungi and its utilization by communities in Genilangit Village, Magetan District,
East Java, Indonesia
Anisa Septiasari, Sugiyarto,
Ahmad Dwi Setyawan
99
xii
Ethnobiology and Socioeconomics
DO-01 Ethnobotany of medicinal plants of indigenous peoples in Kampung Adat Dukuh, Garut District, West Java,
Indonesia
Santhyami, Endah Sulistyawati 99
DO-02 The diversity of useful plants and the botanical knowledge of Rejang tribe people in Kepahiang District,
Bengkulu Province, Indonesia
Wiryono, Sriwahyuni, Gusti Ayu
Winanda, Saprinurdin, Steffanie
Nurliana
99
DO-03 Ethnobotany of food plant used by Sundanese Ethnic in Nyangkewok Village, Sukabumi District, West Java,
Indonesia
Ken Dara Cita, Ratnasari
Hasibuan
100
DO-04 Ethnobotany Amorphophallus paeoniifolius in Cimanuk watershed: Case study in Cisoka Village, Cikijing
Subdistrict, Majalengka District, Indonesia
Asep Zainal Mutaqin, Denny
Kurniadie, Johan Iskandar,
Mohamad Nurzaman, Ruhyat
Partasasmita
100
DO-05 Analysis of changes in wetland management system in Sindang Hamlet, Rancakalong Village, Sumedang
District, West Java, Indonesia
Rahmi Aulia Hidayat, Ruhyat
Partasasmita, Johan Iskandar
100
DO-06 Ethno-ecology of mina padi management in Lampegan Village, Ibun District, Bandung District, West Java,
Indonesia
Iik Nurul Fatimah, Ruhyat
Partasasmita, Johan Iskandar
101
DO-07 Developing mangroves recreation area in Sukakerta Village, Karawang, West Java, Indonesia: Challenges in
building perspective of local community
Tuty Handayani, Fika Afriyani,
Noverita Dian Takarina, Titi
Soedjiarti, Adhitya Anggit
Wicaksono
101
DO-08 The utilization of native starfruit from Indonesia Averrhoa dolichocarpa Rugayah & Siti Sunarti and
Averrhoa leucopetala Rugayah & Siti Sunarti
Frisca Damayanti, Inggit Puji
Astuti, Rizmoon Nurul
Zulkarnaen, Siti Sunarti
101
DO-09 SWOT analysis to determine development strategies of Liwa Botanic Gardens, West Lampung, Indonesia
Trio Santoso, Sugeng P. Harianto,
Niskan W. Masruri, Gunardi D.
Winarno, Machya K. Tsani
102
DO-10 A bibliometric overview of Indonesia’s Biodiversity Research: Social network analysis and future strategic
policy
Irene Muflikh Nadhiroh, Tri
Handayani, Mia Amelia, Rizka
Rahmaida, Ria Hardiyati
102
DO-11 The role of women in fishing villages in handling plastic waste on the coast of Tuban, East Java, Indonesia
Dewi Hidayati, Nur Syahroni, R.
Haryo Dwito Armono, Putu Dana
Karningsih, Nova Maulidina
Ashuri, Suwarsih, Marita Ika
Joesidawati
103
DO-12 Community knowledge of land-use change around oil palm plantation
Emi Roslinda, Dwi Astiani, Burhanuddin, Hanna Artuti
Ekamawanti, Wiwik Ekyastuti
103
DO-13 Plant diversity as potential to Timber Forest Products (TFPs) and Non-Timber Forest Products (NTFPs) in
Karst Ecosystem, Grobogon, Central Java, Indonesia
Gilang Dwi Nugroho, Aditya,
Sunarto, Widhi Himawan,
Chynthia Permata Sari, Ganes
Regitha Sukma Pramesthi,
Natasha Nancy
103
xiii
DO-14 The growth and yield performance of true shallot seed production in Central Sulawesi, Indonesia
Saidah, Andi Nirma Wahyuni,
Muchtar, Syafruddin, Andi
Irmadamayanti, Irwan S. Padang,
Heni SP Rahayu
104
DO-15 The growth and yield performance of various new high yield varieties of hybrid maize in Sigi District, Central
Sulawesi
Saidah, Andi Irmadamayanti,
Heni SP Rahayu, Muchtar, Irwan S. Padang, Andi Nirma Wahyuni,
Syafruddin
104
DP-01 Antibacterial activity test of forest honey from Apis dorsata bee species origin of South Kalimantan and West
Kalimantan, Indonesia
Yelin Adalina, Pindi Prayoga,
Ade Heri Mulyati
105
DP-02 Study ethnobotany of "ketak" (Lygodium circinnatum) and its utilization as crafts in Indonesia
Mulyati Rahayu, Emma Sri Kuncari, Mahdawia, Marwan
Setiawan
105
DP-03 Utilization of ‘Benda’ (Artocarpus sp.) as alternative food in Bogor, Indonesia
Siti Susiarti, Mulyati Rahayu,
Emma S. Kuncari, I. Puji Astuti
105
DP-04 Introduction of soybean cultivation technology in rainfed areas to increase productivity and farmers’ income
Dian Adi Anggraeni Elisabeth,
Agustina Asri Rahmianna, Arief
Harsono
105
Bioscience
EO-01 Optimization of bioactive compounds of wood vinegar from Shorea pachyphylla
Hasan Ashri Oramahia, Elvi
Rusmiyanto, Kustiati
106
EO-02 Lead toxicity effect on physio-anatomy of bead-tree, jatropha, castor bean and Philippine tung grew in water
culture
Hamim, Hanifatunisa,
Hadisunarso, Luluk Setyaningsih,
Deden Saprudin
106
EO-03 The effect of NAA, ascorbic acid hormone and nutrition combination for tapping panel dryness in Hevea
brasiliensis clon PB 260 and IRR 42
Ade Fipriani Lubis, Rosmayati,
Radite Tistama, Revandy A.M.
Damanik
106
EO-04 Conservation of Phalaenopsis amabilis by orchid seeds storage
Dwi Murti Puspitaningtyas,
Elizabeth Handini
107
EO-05 Comparative skin histology of striped catfish (Pangasius hypophthalmus) at different age
Dewi Hidayati, Moh. Ulya
Alfarisy, Fredy Kurniawan, Endry
Nugroho Prasetyo
107
EO-06 Preference and biology of Tribolium castaneum Herbst. (Coleoptera: Tenebrionidae) on five corn varieties in
grain and flour form
Ludji Pantja Astuti, Rina
Rachmawati, Mutala'liah, Yunita
Eka Lestari
107
EO-07 Effects of black soy phytoestrogens (Glycine soja) against elevated levels of estradiol in rat blood (Rattus
norvegicus) ovariectomy
Dwi Yuliawati, Wuri Widi Astuti,
Fitri Yuniarti
108
EO-08 Histopathology of gill tilapia (Oreochromis niloticus) infected by Trematoda monogenean Dactylogyrus sp.
ectoparasite
Syela Paramesti Kusuma, Agung
Budiharjo, Tetri Widiyani
108
xiv
EO-09 In vitro evaluation of culturable bacteria from pineapple (Ananas comosus) for their drought-tolerant capability
Rahayu Fitriani Wangsa Putrie, I
Nyoman Pugeg Aryantha,
Iriawati, Sarjiya Antonius
108
EO-10 Biomass allocation of rice (Oryza sativa) with varying soil salinity related to growth stages
M. Muryono, Nafisah, I.
Trisnawati, N. Habibi
109
EO-11 Investigation of nitrogen uptake ability of mangrove species by chlorophyll meter measurements
M. Muryono, Indah Trisnawati 109
EO-12 Allelopathic activity of Dendrophthoe pentandra as a potential bioherbicide to control seed germination and
growth of Eleusine indica
Lutfan Alharits, Niarsi Merry
Hemelda, Yasman, Windri
Handayani
109
EO-13 Utilization of drone technology for the design of yard use in Cinanjung, Village Sumedang, West Java, Indonesia
Sofiatin, Hikmat Ramdan, Yooce
Yustiana, Angga Dwiartama, Ira
Rahmayunita, Olga Fatriana
109
EO-14 Designing and validation of a questionnaire assessing knowledge, attitude, and practices about caging backyard
in order to prevent influenza human (H5N1)
Risqa Novita, Ridad Agoes 110
EO-15 Length-weight, maturity, and condition factor of Torpedo scads (Megalaspis cordyla Linnaeus, 1758) in Java Sea,
Indonesia
Dian Oktaviani, Eko Prianto,
Duto Nugroho
110
EO-16 Microbiological analyses of honey water jelly beverages containing probiotic and mangosteen peel extract
Nilam Fadmaulidha Wulandari,
Novik Nurhidayat, Titin Yulinery,
Nandang Suharna
110
EO-17 The effect of red turi (Sesbania grandiflora) leaf extract on the amount of bacterial colonies and IL-6 in
postpartum mice inoculated by Staphylococcus aureus
Wuri Widi Astuti, Rahma Dian
Hanifarizani, Endah Kamila
Mas’udah, Anna Malia, Sanarto
Santoso, Sri Poeranto,
Noorhamdani, Pande Made
Dwijayasa
111
EO-18 Production of secondary metabolites and antibacterial activity during the growth period of endophytic fungi
isolated from gall rust sengon plants
Noor Rahmawati, Alfi Rumidatul,
Sopandi Sunarya
111
EO-19 Isolation and identification fungi inhabiting impregnated wood, and their role of quality changing at rubber
impregnated wood
Noor Rahmawati, Ihak Sumardi,
Rudi
112
EO-20 Evaluation of bio-attractant and matrix bait from organic waste for termite bait
Yuliati Indrayani, Sofwan Anwari 112
EP-01 The toxicity of Hg, Cd and Pb to the development of gonads mussels (Perna viridis) as biomarker histological
for pollution
Yulia Irnidayanti, Shofy Septiana 112
EP-02 The effect of major invasive species attack on rice production in East Kalimantan, Indonesia
Wawan Banu Prasetyo 113
EP-03 Effects of sengon (Falcataria moluccana) bark methanol extract as anthelmintic against Ascaris suum
Alfi Rumidatul, I Nyoman P.
Aryantha, Endah Sulistyawati
113
xv
EP-04 Seed germination, seedling survival and storage behavior of Koompassia excelsa (Leguminosae)
Dian Latifah, Fitri Fatma
Wardani, Rizmoon Nurul
Zulkarnaen
113
EP-05 Resistance of oil palm trunk lumber impregnated by phenol-formaldehyde and oil palm fruit shell ash
nanofiller by Cryptotermes cynocephalus Light.
Atmawi Darwis, Anne Hadiyane,
Tati Karliati
114
EP-06 Dimensional stability and mechanical properties of sawdust Toona sinensis filler and pine resin-impregnated
Oil Palm Trunk Lumber (OPTL)
Anne Hadiyane, Tati Karliati,
Atmawi Darwis
114
EP-07 Toxicity test of combination of temulawak (Curcuma xanthorrhiza) and belimbing wuluh (Averrhoa bilimbi)
juice on kidney histopathology of rats (Rattus
norvegicus)
Kartiawati Alipin, Dika Widia
Nur Azizah, Nining Ratningsih
114
EP-08 The microscopic structure of kidney wistar rat diabetes mellitus under the effect of jengkol (Archidendron
pauciflorum) fruit peel ethanolic extract
Desak Made Malini, Nining
Ratningsih, Madihah, Adnin
Laila, Wawan Hermawan.
115
EP-09 Spatio-temporal monitoring land surface temperature using Landsat 8 Satelite: A case study in Surakarta and
Karanganyar, Indonesia
Sapta Suhardono, Ahmad Dwi
Setyawan
115
Keynote Speech
Diversity, resource and conservation of tropical anguillid
eels in Southeast Asia
Takaomi Arai 115
Note: A. Genetic Diversity, B. Diversity of Species, C. Diversity of Ecosystem, D. Ethnobiology and Socioeconomics, E.
Bioscience (Life Science and Technology); O. Oral, P. Poster; AA. Keynote speech
AA-01
v
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ABS SOC INDON BIODIV Vol. 6, No. 3, November 2019 ISSN: 2407-8069 Pages: 67-116 DOI: 10.13057/asnmbi/m060301
ABSTRACT
International Conference on Biodiversity
Society for Indonesian Biodiversity (SIB)
Surakarta, Indonesia, 11-12 November 2019
Genetic diversity
AO-01 Morphometric diversity and genetic relationship
analyze to determine potential indigenous
buffaloes of Banten, Indonesia
Dewi Murni1,2,♥, Umie Lestari1, Sri Endah Indriwati1,
Achmad Efendi3, Nani Maryani2, Mohamad Amin1 1 Department of Biology, Universitas Negeri Malang. Jl. Semarang 5,
Kota Malang 65145, East Java, Indonesia 2 Department of Biology Education, Universitas Sultan Ageng Tirtayasa.
Jl. Raya Jakarta, Km. 4, Kota Serang 42124, Banten, Indonesia. 3 Department of Statistics, Universitas Brawijaya. Jl. Veteran, Kota
Malang 65145, East Java, Indonesia.
This study aimed to describe the morphometric diversity
and genetic relationship among indigenous buffaloes in
Banten, Indonesia. In this study, 125 heads of buffaloes
from six regions were investigated based on 11
morphometric characters. Morphometric diversity was
analyzed using multivariate discriminant analysis. The
Euclidean genetic distances were used to estimate the
genetic relationship among the buffalo populations. The
indigenous buffaloes of Banten showed high morphometric
diversity with a coefficient ranging from 2.83 to 41.43%.
The body length and chest circumference can be used as a morphometric marker to determine potential indigenous
buffaloes with a high coefficient value (0.546). The Serang
District buffaloes have the highest mean of body length and
chest circumference, which shows that this population is
more potent compared to the other regions. The
morphometric characteristics of buffaloes from Serang
City, Cilegon City, Serang District, and Pandeglang
District were likely to be homogeneous, while Lebak and
Tangerang District populations tended to be heterogeneous.
The proximate genetic relationship was found between
Serang and Pandeglang District's populations. The results of the analysis indicated that morphometric diversity and
genetic relationships among buffalo populations were
associated with geographical origins. The current
information can assist in potential decision making on
buffaloes genetic resources management and conservation.
Euclidean distance, genetic relationship, indigenous
buffaloes, morphometric diversity, multivariate
discriminant
AO-02 New DNA barcode primer assembly for
Indonesian Bamboo
Annisa♥, Rini Hafzari, Tia Setiawati, Joko Kusmoro
Department of Biology, Universitas Padjadjaran. Jl. Raya Bandung-
Sumedang Km. 21, Jatinangor, Sumedang 45363, West Java, Indonesia
Indonesia has high diversity of Bamboo species. The use of
bamboo has been known for centuries, from cooking
utensils to house building material; from tea to cosmetics. As a versatile plant, it is very susceptible to over
exploration. Harvesting bamboo without re-planting has
been an issue that not only affects ecological niche but also
genetic. DNA barcode can be used as a tool to study A and
also product standardization for industry. Here we present
the result of primer assembly from one of the genes that is
recommended as DNA barcode, ribulose-bisphosphate
carboxylase (rbcl). In general, this primer is able to use as
DNA barcode. The primer was able to differentiate species
in Genus Schizostachyum used in this research and made a
monophyletic group. Some species were shown to be sister taxa. In our case, rbcl can be recommended as DNA
barcode for bamboo.
Barcode, rbcL, bamboo, genus
--------------------
Note: In order to avoid improper conduct of third parties against authors
by using email addresses, starting on 2018 correspondence emails (♥) are
not listed. Colleagues can communicate with the author by mail or contact
us at [email protected]
ABS MASY BIODIV INDON, Surakarta, 11-12 November 2019, hal. 67-116 68
AO-03 Genetic diversity of sago palm (Metroxylon sagu)
accessions based on plastid cpDNA matK gene as
DNA barcoding
Barahima Abbas1,♥, Ihwan Tjolli1, Munarti2 1 Faculty of Agriculture, Graduate School, Universitas Papua. Jl. Gunung
Salju, Manokwari 98314, West Papua, Indonesia 2 Faculty of Teacher Training and Education Science, Universitas Pakuan.
Jl. Pakuan, Bogor 16143, West Java, Indonesia
Metroxylon sagu Rottb.) is one of plant species including in
the Palmae family that deposits starch in the trunk. Sago palm was reported high capability to produce large
amounts of starch and large variation starch qualities. The
objectives of this study are revealed sequence chloroplast
DNA (cpDNA) associated with matK genes and revealed
Genetic diversity of sago palm accessions. Plant materials
used in the studies were derived from Sago Research
Center (SRC) collection. DNA extraction has adopted the
procedure of Plant Genomic DNA Mini Kit. Polymerase
chain reaction (PCR) was performed using matK primer
sets. DNA PCR product was sequenced by 1st Base Asia,
Singapore. Results of study were registered ten of 15
sequences of matK gene that were observed into the GenBank, NCBI as DNA barcoding and authenticity of
sago palm germplasm from Papua islands, Indonesia
territorials. Sequence cpDNA associated with matK genes
in the genome of sago palm were shown differences among
accessions. Molecular diversities of sago palm accessions
based on matK gene showed sago palm accessions
separated into two genotypes. Genotype-1 incorporated ten
individuals of 15 accessions and genotype-2 incorporated
five individuals of accessions. Genotype-2 was observed
mutations occur in the sequences of matK gene.
Chloroplast DNA, DNA barcoding, matK gene, sago palm
AO-04 Morphological diversity and the cultivation
practices of Laportea decumana as a medicinal
plant in the highland area of Puncak District,
Papua Province, Indonesia
Marpen Y. Tabuni, Saraswati Prabawardani♥, Nouke
L. Mawikere
Faculty of Agriculture, Graduate School, Universitas Papua. Jl. Gunung
Salju, Manokwari 98314, West Papua, Indonesia
Laportea decumana (Roxb.) Wedd (Itching leaf plant) is
suspected to spread in various regions of Papua. For the
people of Papua, L. decumana is one of the traditional
medicines that have long been used. The leaves are efficacious in curing headaches, aches, pains and various
other types of diseases. People in the central highlands of
Papua, especially in Puncak Region, have used L.
decumana leaves as traditional medicine for hundreds of
years ago. Previously the community only relied on forests
or nature to get L. decumana, but to make it easier to
obtain, the native communities in Puncak region had
traditionally cultivated L. decumana at this time. The
cultivation system is based on their traditionally hereditary
habits. However, information about the L. decumana
cultivation system in Puncak region has not been
documented. Likewise, information on the diversity of L.
decumana is still limited, so research on identifying this
plant species needs to be done considering the
morphological character of these plants is quite varied. The
purpose of this study was to identify the morphological character of L. decumana in the Sinak area of Puncak
District, Papua Province, Indonesia and examine the itch
leaf cultivation system by the local community. This
research was conducted for 3 months, from October to
December 2018. This study used a descriptive method with
surveys, observation and interview techniques. The
research location was determined intentionally, namely the
Sinak region in 4 subdistricts. In each subdistrict, 3 villages
were taken as samples that cultivate L. decumana. Each
village consists of 5 respondents, namely farmers, tribal
leaders (village chiefs), religious leaders, and people who understand the type of L. decumana, so we get 60
respondents. Data analysis was carried out by tabulation,
and to cluster the types of L. decumana plants based on
their similarity characters, cluster analysis was used using
NTSYS. From the results of the study, there were 9 (nine)
accessions of L. decumana consisting of 8 accessions of
shrub/sub-shrub plants and 1 accession of trees. A total of 4
accessions are cultivated in the home yard and garden
traditionally from land preparation, planting, maintenance
to harvest, and 5 other accessions in the form of wild. The
results of cluster analysis showed that L. decumana which had a high degree of similarity was the accessions of Abe
ponim-2 and Ponim lambuh-2 by 90%, while the degree of
similarity characters which is quite far was found between
the accession of Ponim katty katty-1 and Towo ponim by
37%
Cultivation, Laportea decumana, medicinal plant,
morphology, similarity
AO-05 Spatial modeling for kedondong (Spondias dulcis)
cultivation in Central Part of Sumatra, Indonesia
Ibna Hayati1,♥, Alex Hartana2, Nina Ratna Djuita2 1 Plant Biology Graduate Program, Department of Biology, Institut
Pertanian Bogor (IPB University). Jl. Raya Dramaga, Campus IPB, Bogor
16680, West Java, Indonesia 2 Department of Biology, Institut Pertanian Bogor (IPB University). Jl.
Raya Dramaga, Campus IPB, Bogor 16680, West Java, Indonesia
Kedondong (Spondias dulcis Parkinson) is a fruit plant
native to Society Islands and widely cultivated in all
tropical regions and countries. However, little is known
about the occurrences and potential distribution of
kedondong as well as its adaptability to different climates in central part of Sumatra. This is the first paper that tried
to reveal spatial modeling distribution of kedondong in
central part of Sumatra. The maximum entropy (MaxEnt)
ABS SOC INDON BIODIV, Surakarta, 11-12 November 2019, pp. 67-116 69
model was used to analyze and predict its distribution and
to develop preliminary habitat suitability map for
Kedondong in central part of Sumatra. The results showed
that the MaxEnt model can be used to study the climatic
suitability for Kedondong cultivation. The most suitable
area identified in this study covers the west coast of
Sumatra and the central part of Sumatra but does not reach
the eastern coast of Sumatra. Notably, the size of the
current potential distribution is significantly larger than the
present occurrence of kedondong in central part of Sumatra. The MaxEnt model performed better than random
with an AUC value of 0.981. Although kedondong is still
largely ignored by scientific community and its potential
has not been deeply explored but it is very important to
develop kedondong germplasm resources which have
adaptability to extreme climate in central part of Sumatra
that differ from its native area.
Central part of Sumatra, cultivated plant, distribution,
kedondong, spatial modeling
AO-06 Shade stress in various growth phases and its
effect on agronomic characters and chlorophyll
content of peanut genotypes
A. Farid Hemon♥, Sumarjan, Hanafi Abdurachman
Faculty of Agriculture, Universitas Mataram. Jl. Majapahit, Mataram
83115, West Nusa Tenggara, Indonesia
This research aimed to study the effect of shade stress in
various growth phases on agronomic character and
chlorophyll content of peanut genotypes. This experiment
used a Complete Random-Split Plot Design. The treatments
were shade at various growth phases and genotypes. Shade
stress consisted of: no shade, shading from planting
beginning to harvest, shade from planting beginning until
the age 25 days after planting (dap) (vegetative phase),
shade from 26 dap until 50 dap (flowering phase until pods
formation), shade from 51 dap to 75 dap (seed filling phase), and shade from 75 dap to 100 dap (seed maturation
phase until harvest). Shading was carried out using black
paranet with a sunlight barrier 35%. The genotypes used
were F3 generation plants from crosses (G200-I and G300-
II x cv.Domba, Takar1, Bison). The results showed that
shading at various growth phases with genotypes was not
significantly interaction on agronomic traits, but there were
significant interactions on chlorophyll-a content and total
chlorophyll. All peanut genotypes (12 genotypes) tended to
produce higher chlorophyll-a content and total chlorophyll
in shade given at planting beginning to harvest. Shade at various plant growth phases had a significant effect on
agronomic character. Shade given from planting beginning
to harvest produced the lowest dry pod weight (12.9
g/plant) or yield decreased until 23.7% compared to peanut
grown without shading. The genotypes significantly
affected on agronomic traits and g6 genotype (G200-I x
cv.Domba) produced the heaviest dry pod weight 17.2
g/plant.
Chlorophyll, genotypes, growth phases, shade
AO-07 Erythrina fusca Lour. on Mount Merapi, Central
Java, Indonesia
ILG Nurtjahjaningsih1,♥, AYPBC Widyatmoko1,
Purnamila Sulistyawati1, Bangun Bramantya2, Putu
Dhian Budhami2, Lukman Hakim1, Yayan Hadiyan1,
Liliek Haryjanto1, Sugeng Pudjiono1, Dedi Setiadi1, Y.
Wibisono1 1Center for Forest Biotechnology and Tree Improvement Research and
Development. Jl. Palagan Tentara Pelajar Km.15, Purwobinangun, Pakem,
Sleman 55582, Yogyakarta, Indonesia 2Gunung Merapi National Park. Jl. Kaliurang Km 22,6, Hargobinangun,
Pakem, Sleman, 55581, Yogyakarta, Indonesia
Erythrina fusca Lour. is distributed along a ridge of Mount
Merapi, Java, Indonesia in altitudes between 1300-1500 m
above sea level. It is an important species for fire-break.
Their habitats are threatened by volcanic eruptions. This study aimed to assess the remaining genetic diversity and
structure among populations of E. fusca on Mount Merapi.
Leaf samples were collected from six populations of 3
forest resorts. Genetic analysis was carried out using 47
polymorphic loci of 7 RAPD markers. The results showed
the HE values ranged between 0.305 and 0.382; suggesting
in a moderate level of genetic diversity; it was higher than
other Erythrina spp. The dendrogram analysis showed the
populations clustered only into a subdistrict i.e. Musuk
Cepogo. The AMOVA analysis also confirmed there was
no genetic diversity found among forest resorts. The low
genetic structure indicated fewer barriers among populations. Restoration areas on Mount Merapi have been
established from the mixed-seedlings from various seed
sources without genetic information that could lose the
genetic characteristics within populations. Frugivores birds
also have contributed to expanding the dispersal of the
seeds of E. fusca on Mount Merapi. This genetic
information has implications for conservation and
management of natural genetic resources.
Genetic structure, RAPD, restoration
AO-08
Genetic diversity of tesek (Dodonaea viscosa Jacq.)
from Mount Merapi based on Random Amplified
Polymorphic DNA markers
Purnamila Sulistyawati 1,♥, AYPBC Widyatmoko1, Ilg
Nurtjahjaningsih1, Liliek Haryjanto1, Yayan Hadiyan1,
Lukman Hakim1, Dedi Setiadi1, Sugeng Pudjiono1, Toni
Herawan1, Yohannes Wibisono1, Bangun Baramantya2,
Dhani Suryawan2 1Center for Forest Biotechnology and Tree Improvement Research and
Development. Jl. Palagan Tentara Pelajar Km.15, Purwobinangun, Pakem,
Sleman 55582, Yogyakarta, Indonesia 2Gunung Merapi National Park. Jl. Kaliurang Km 22,6, Hargobinangun,
Pakem, Sleman, 55581, Yogyakarta, Indonesia
ABS MASY BIODIV INDON, Surakarta, 11-12 November 2019, hal. 67-116 70
Tesek (Dodonaea viscosa Jacq.) is naturally distributed at
the steep-slope of Mount Merapi, Java, Indonesia at an
altitude between 1,200-3,000 m above sea level. It is an
important species for traditional ceremonies and medicine.
The population distributions are affected by volcano
activities. This study aimed to obtain data and information
on genetic diversity and structure of Tesek populations on
Mt. Merapi. Leaf samples were collected from 5 natural
populations of 4 forest resorts in Mt. Merapi and 1
population from Mt. Merbabu as an outgroup. Genetic analysis was conducted using 36 polymorphic loci of 7
RAPD markers. The genetic diversity value (HE) within
the population ranged between 0.2419-0.3532; meaning a
moderate level of genetic diversity. There was no private
allele that characterized within a population. A dendrogram
analysis showed a genetic relationship among populations
concordant with their geographic distant; indicating a
barrier of gene flow among populations. The AMOVA
analysis also confirmed the gene flow barrier; it showed a
significant value of about 8% of genetic diversity was
found among forest resorts. This genetic diversity information data can be used as a baseline to develop the
Tesek conservation in the Mt. Merapi areas, especially for
genetic-based land conservation.
AMOVA, dendrogram, genetic structure, genetic
relationship
AO-09 Genetic similarity of 35 cassava (Manihot esculenta)
clones by RAPD
Rani Agustina Wulandari1,♥, Qonita Luthfi Nur
Hanifah1, Christin Triagustina1, Aisyah Fitri Rohani1,
Patrisia Citra Saraswati1, Tri Harjaka2 1Department of Agronomy, Universitas Gadjah Mada. Jl. Flora No.1,
Bulaksumur, Sleman 55281, Yogyakarta, Indonesia 2Department of Plant Protection, Universitas Gadjah Mada. Jl. Flora No.1,
Bulaksumur, Sleman 55281, Yogyakarta, Indonesia
Cassava (Manihot esculenta Crantz) is widely grown in
various regions in Indonesia. Cassava is a type of plant
with a tendency towards low variety because it is
commonly propagated vegetatively by cuttings. Clone
names of cassava are distinguished according to the area
name of origin, or according to taste, and author desire.
Based on the differences in clone names, there is a concern
that cassava which has different names has same genotype.
Thus cassava identification using molecular markers
(RAPD) is essential to determine its genetic
variety/similarity level. The aim of this research to determine genetic similarity of 35 cassava clones based on
molecular analysis using RAPD marker. All of the cassava
clones were collection of Faculty of Agriculture,
Universitas Gadjah Mada that had been collected by Dr. Tri
Harjaka S.P, M.P. The research was conducted in January
2018 until April 2018 in Mendel Room, Plant Breeding
Laboratory, Department of Agriculture Cultivation, Faculty
of Agriculture, Universitas Gadjah Mada, Yogyakarta.
RAPD used in this research involved 10 primers. The result
of DNA band was observed and then analyzed by NTSYS
software. The research result showed that based on the
dendrogram among 35 cassava clones, the highest
similarity coefficient was showed between clone LG and
LL (86%), next closer genetic was showed by LE-LJ
(83%), and than LI and LB (78,5%). All of cassava clones
were divided into four groups. It was concluded that 35
cassava clones have various genetic information, two of
them very close and can be stated as one clone.
Cassava, clone, RAPD, similarity
AO-10 Bacillus spp. strain from Indonesian honey and
other-bee associated products as probiotic
candidates
Stella Magdalena1,♥, Gracia Nathalie1, Ivana
Purnawidjaja2, Yogiara3 1Department of Food Technology, Universitas Katholik Atma Jaya. BSD
Campus, Jalan Raya Cisauk Lapan 10, Sampora, BSD City, Tangerang
15345, Banten, Indonesia 2Department of Biology, Universitas Katholik Atma Jaya. BSD Campus,
Jl. Raya Cisauk Lapan 10, Sampora, BSD City, Tangerang 15345, Banten,
Indonesia 3Department of Biotechnology, Graduate School, Universitas Katholik
Atma Jaya. Semanggi Campus, Jl. Jenderal Sudirman 51, Jakarta 12930,
Indonesia.
Honey and other honey bee products are well known for
their nutritional and medical capabilities as antibacterial,
antifungal, and anti-inflammatory agents. However, there
have been only few research studies on microorganisms
isolated from honey in Indonesia. In this study, a total of
115 bacterial strains were isolated from honey (5 samples),
bee pollen (5 samples), beehive (6 samples), and propolis
(4 samples) from various bee species, floral origins, and
local farms in West Java, Indonesia. Six isolates
demonstrated antifungal activity against pathogenic fungal, Candida albicans and Aspergillus fumigatus. These six
bacterial strains (L30, L35, Y12, Y21, Y23, Y33) were
identified by partial 16S rRNA gene sequencing analysis.
L30 and L35 were similar to Bacillus subtilis, Y12 and
Y33 were similar to Bacillus velezensis, while Y21 and
Y23 were similar to Bacillus amyloliquefaciens. All
Bacillus isolates exhibited no hemolytic activity, except B.
velezensis Y33 that showed β-hemolysis activity.
Furthermore, the isolates showed amylolytic activity that
could be important for probiotics property. Thus, all
isolates used in this study were able to survive pH 2.5, 1% (w/v) of bile salts, and 0.2% (v/v) of phenol except B.
subtilis L35. Based on these results, Bacillus strains
showed promising probiotic candidates. Further probiotic
property screening is required to examine the ability of
each strain to become a fully health-conferring probiotic.
Bacillus, Indonesian honey, probiotic, safety
ABS SOC INDON BIODIV, Surakarta, 11-12 November 2019, pp. 67-116 71
AO-11 Identification and pathogenicity of Colletotrichum
spp. associated with chili anthracnose in
Yogyakarta, Indonesia
Dhika Sri Anggrahini♥, Arif Wibowo, Siti Subandiyah
Department of Plant Protection, Universitas Gadjah Mada. Jl. Flora No.1,
Bulaksumur, Sleman 55281, Yogyakarta, Indonesia
Anthracnose caused by Colletotrichum spp. is among the
numerous disease-causing low production and quality of
chili. A number of Colletotrichum isolates from
anthracnose symptoms were collected from chili cultivated
in five regencies in Special Region of Yogyakarta, namely
Bantul, Gunung Kidul, Kulon Progo, Sleman, and Yogyakarta (Jogja), in addition, Magelang as comparative
isolate collected from Central Java. A total of 14
Colletotrichum isolates were observed and screened based
on morphology using UPGMA. Conidial shape was
fusiform to cylindrical with two ends acute or one end
slightly obtuse, and falcate in various size. Conidial size
ranged 9.02-19.38 µm x 2.37-8.57 µm. At level of 0.85
similarities, 8 isolates were selected as representative
isolates including one comparative isolate, and then
analyzed in molecular level. Multi-gene phylogenetic tree
was generated using ITS1-4, gapdh, tub2 genes to reveal the species among Colletotrichum isolates. Phylogenetic
analysis delineated B1, G1, and K2 as C. scovillei; J1 as C.
truncatum; S1 and S2 as C. siamense. Isolates of Jogja 2
(J2) and Magelang (Mg) were distinct from chili
Colletotrichum ex-type or references adopted.
Pathogenicity assay on chili fruit showed that Mg isolate
had the highest virulence on wounded fruit, compared to all
other isolates. The existence of highly virulence species
such as Mg isolates threatened biosecurity of the country.
Anthracnose, Colletotrichum, chili
AO-12
Realized genetic gain on oil productivity resulted
from breeding program of cajuput and its impact
on farmer’s income
Sumardi♥, Noor Khomsah Kartikawati, Prastyono, Arif
Nirsatmanto, Anto Rimbawanto
Center for Forest Biotechnology and Tree Improvement Research and
Development. Jl. Palagan Tentara Pelajar Km.15, Purwobinangun, Pakem,
Sleman 55582, Yogyakarta, Indonesia
Cajuput (Melaleuca cajuputi subsp. cajuputi) is a potential species to improve community welfare by refining its
leaves into oil. Two important traits for increasing cajuput
productivity are oil yield and leaf biomass. Therefore,
breeding for improving these traits is necessary and first-
generation breeding program imposing selection on oil
yield was established. This paper presents realized genetic
gain on oil productivity from the breeding program
accompanied by its impact and other traits of leaf biomass.
Observation was conducted in gain trial consisting of trees
from two improved seed sources (ISS) and trees from three
unimproved seed sources (USS) as genetic checks. Trees
from each seed sources were planted in 20 squared tree-
plots, repeated in 8 blocks, spacing of 3x3 meters.
Measurement was conducted at 12 years of age. Result of
study showed that oil yield from ISS was higher than from
USS with realized genetic gain ranged from 17.49% to
31.32%. Although it was accompanied with 6.64% to
15.35% lower on leaf biomass than USS, combined
analysis between the two traits showed that ISS produced higher oil productivity per hectare at around 600 kilograms
oil per hectare per year, and making it more profitable to
increase farmer’s income than USS.
Biomass, cajuput, genetic gain trial, income, oil
productivity
AO-13 Morphological and molecular characterization of
Kryptopterus bicirrhis from Riau, Indonesia
Roza Elvyra♥, Meyla Suhendra, Widya Hidayana
Department of Biology, Universitas Riau. Jl. HR. Soebrantas Km. 12,5,
Kampus Bina Widya, Simpang Baru, Tampan Pekanbaru 28292, Riau,
Indonesia
The research on morphology and molecular
characterization has been conducted on Kryptopterus
bicirrhis from Riau Province, Indonesia. The identification
of species in the genus Kryptopterus, specifically K.
bicirrhis, is based on 23 morphological characters. This
study provides several morphological descriptions of K.
bicirrhis and also molecular characters with the COI gene
marker. The results of the Polymerase Chain Reactions are
partial cytochrome oxidase-1 gene of 655 bp. Blast analysis
of the K. bicirrhis COI gene sequence showed an identity of 88.66% with K. bicirrhis from GenBank data. The
sequence of Polymerase Chain Reactions product of partial
COI gene was done multiple alignments with other fish
from Genbank and analyzed using MEGA software of
version 6.0. The genetic distance and the phylogenetic tree
using the Neighbor-Joining method based on the sequence
of COI gene can be used to differentiate K. bicirrhis from
other fish species.
COI gene, Kryptopterus bicirrhis, morphology, Riau
AO-14 Variation of prolactin gene in the Indonesian
Frisian-Holstein cattle
Paskah Partogi Agung1,♥, Ari Sulistyo Wulandari1,
Sharon Hillary2
1Research Center for Biotechnology, Indonesian Institute of Sciences.
Cibinong Science Center, Jl. Raya Bogor Km. 46, Cibinong, Bogor
16911, West Java, Indonesia 2Graduate School, Universitas Jenderal Soedirman. Purwokerto,
Banyumas, Central Java, Indonesia
ABS MASY BIODIV INDON, Surakarta, 11-12 November 2019, hal. 67-116 72
Prolactin is a polypeptide hormone, encoded by the PRL
gene, synthesized and secreted by anterior pituitary, and
affecting milk yield and composition. This study was
conducted to identify the PRL gene polymorphism in the
Indonesian FH cattle. A total of 139 individual cattle blood
samples from West Java were used to obtain DNA samples
through the DNA extraction process. Identification of the
PRL gene was performed using PCR-RFLP method with
RsaI restriction enzyme. The PRL gene was amplified
using forward primer 5'-CCAAATCCACTGAATTATGCTT-3' and reverse primer
5'-ACAGAAATCACCTCTCTCATTCA-3'. The PRL gene
in the Indonesia FH cattle was polymorphic based on the
PCR-RFLP analysis but the heterozygosity value was low.
There were two alleles (G and A) and three genotypes (GG,
GA, and AA) identified in the PRL gene of the Indonesian
FH cattle. The PRL gene genotype frequencies were 0.914,
0.079, and 0,007 for GG, GA, and AA genotypes
respectively. Information about the PRL gen polymorphism
in this study can be considered for further study to analyze
its association with milk yield trait.
FH; Identification; Indonesia, PRL; Polymorphism;
AO-15
Pollen fertility of F1 crossing local rice with
drought-tolerant rice
Aditya Wahyudhi♥, Panjisakti Basunanda
Faculty of Agriculture, Universitas Gadjah Mada. Jl. Flora No.1,
Bulaksumur, Sleman 55281, Yogyakarta, Indonesia
Pollen fertility is an important parameter that influences
percentage of seedset. The high percentage of pollen
fertility is caused a high percentage of filled grain in rice.
The coloring pollen is method to find out pollen fertile by
detecting the starch content in pollen. This study aims to
determine the pollen fertility and the percentage filled grain
of F1 results from local rice crossing with drought-tolerant
rice. This research was conducted at the Plant Genetics and
Plant Breeding laboratory and the greenhouse of the
Faculty of Agriculture, Gadjah Mada University, Yogyakarta, Indonesia. Observation of pollen fertility and
percentage of filled grain was carried out on seven
combinations of F1. The crossbreeds are 'Bluebonnet' vs.
'Kasalath', 'Bluebonnet' vs' Mentik Wangi ',' Mentik Wangi
'vs' Bluebonnet', 'Bluebonnet' vs' Mentik Susu ',' Mentik
Susu 'vs' Bluebonnet', ' Kasalath 'vs' Mentik Wangi',
'Mentik Wangi' vs' Kasalath '. The results of staining with
IKI 1% solution showed 'Bluebonnet' vs 'Milk Mentik' had
the highest pollen fertility (84.67%), while the lowest
crossbreeds are 'Bluebonnet' vs 'Kasalath' (27.05) 'Mentik
Wangi' vs 'Bluebonnet' (22.03%), 'Mentik Susu' vs
'Bluebonnet' (30.43%), 'Mentik Wangi' vs 'Kasalath' (39.24%). A low percentage of fertile pollen is followed by
a low percentage of filled grain.
Breeding, filled grain, IKI staining
AO-16 Pod yield and haulm weight peanut cultivars
grown in dry lands with dry climate
Agustina Asri Rahmianna♥, Andy Wijanarko,
Yuliantoro Baliadi
Indonesian Legumes and Tuber Crops Research Institute. Jl. Raya
Kendalpayak Km 8, Pakisaji, Malang 65162, East Java, Indonesia
Peanut (Arachis hypogaea L.) is the third major food crops
in Indonesia after maize and soybean. The seeds contain
oil, protein, and carbohydrate which are benefit to human
health. Despite its health benefits, farmers sell all the pods
in order to get cash. The haulms or fodders which stay
green until harvesting time is an important source for
livestock during dry season. In drylands with dry climate
where there is only one growing season, however, the haulms are left in the field. This is one obvious benefit so
the C-organic content of the soils is high. Drylands with
dry climate such as in East Nusa Tenggara are the potential
lands for peanuts. The experiment was conducted to assess
the pod and haulm yields of eleven peanut cultivars grown
in drylands with low rainfall at Sumba Timur District of
East Nusa Tenggara during February-May 2017. Ten
peanut superior cultivars and one local cultivar are grown
in two sites: Laipori and Palakahembi Villages. A
randomized block design with three replicates was applied
in each site. The treatment was cultivar: Local Sandel,
Bison, Gajah, Hypoma 1, Hypoma 2, Kancil, Kelinci, Takar 1, Talam 1, Talam 2, and Tuban that belonged to
Spanish type expect Kelinci that was a Valencia type. A
100 kg/ha of composite NPK fertilizer was applied at
sowing time, water source was merely from rainfall. The
pesticides were applied three times during the growing
period. The results indicated that the average pod yields of
10 national cultivars from two sites were 1.75-2.57 times
higher than that of Local Sandel. Hypoma 1 cultivar gave
the highest with 2.313 t/ha of dry pod yield s or 157%
higher than that of Local Sandel yield. Instead of producing
high pod yield, Hypoma 1 also produced 6.1 t of fresh haulms/ha and those superior cultivars were 5.3-7.2 t/ha.
These amounts benefited the soil organic materials after
completing the experiment as showed by its high amount of
organic matter: 5-5.2%. These amounts are the ideal
amount of organic matters contained by soils to maintain
soil fertility. In summary, the superior cultivars especially
Hypoma 1 can be proposed to the farmers in Sumba Timur
because of its high pod yields and organic matters content
in soils after the cultivars harvested.
Arachis hypogaea, organic matters, pod yield, superior
cultivars
AO-17 Antixenosis of soybean promising lines and
resistance levels to pod borer, Etiella zinckenella
Marida Santi Yudha Ika Bayu, Gatut Wahyu Anggoro
Susanto♥, Yusmani Prayogo
ABS SOC INDON BIODIV, Surakarta, 11-12 November 2019, pp. 67-116 73
Indonesian Legumes and Tuber Crops Research Institute. Jl. Raya
Kendalpayak Km 8, Pakisaji, Malang 65162, East Java, Indonesia
Pod borer, Etiella zinckenella, is a severe agricultural pest
attacking various leguminous crops including soybean. The
objective of this study was to determine the antixenosis of
soybean genotypes and their resistance level against pod
borer. The experiment was conducted in screen house and
laboratory of Entomology, Indonesian Legumes and Tuber Crops Research Institute, Malang, Indonesia using
Randomized Block Design with four replicates. The
number of 16 soybean promising lines were used as
treatment. Planting dates were arranged in such a way to
synchronize the time of flowering of 16 soybean promising
lines. A pair of four days emerged adult pod borer were
infested into each plant at R4 stage of the soybean (21 days
after flowering) for two days. The number of egg was
observed two days after infestation (DAI) and the number
of larva and damaged intensity were observed at 14 DAI.
The result showed that the difference of soybean promising lines was significantly affected egg and larval population
and pod damage intensity. The lowest egg (1 egg/plant)
was found on GH-Gn-8 and the lowest larval population (0
individual/plant) was found on GH-Gn-8 and GH/KR-13.
Three soybean promising lines were recorded no pod
damaged by the larva, namely Brg/MLG-12, Brg/Myp-13,
and Grb/Myp-16. Seed weight varied from 2.0-6.9 gr/plant.
Trichome density varied from 24-50/mm2 with three-
position (lying, skewed, and upright). In conclusion, there
were three soybean promising lines showed consistently
resistant against pod borer, namely GH-Gn-8, GH/KR-13,
and Grb/Myp-16. The resistance of these promising lines was antixenosis or non-preference as place to lay eggs and
as feed. These three soybean promising lines are potential
as gene source for improving the resistance against pod
borer.
Antixenosis, damaged intensity, Etiella zinckenella,
resistance, soybean promising lines
AO-18 Physical and chemical properties of sixty peanut
germplasm
Erliana Ginting♥, Novita Nugrahaeni
Indonesian Legumes and Tuber Crops Research Institute. Jl. Raya
Kendalpayak Km 8, Pakisaji, Malang 65162, East Java, Indonesia
Nutrient values of peanut (Arachis hypogaea L.),
particularly protein and fat is essential in terms of
predominant use of peanut for foods. The seed size is also
important for end use of the products. The nutrient contents
and the seed size may differ between cultivars as well as in peanut germplasm. In terms of using the germplasm
collection for breeding purposes as well as for completing
the peanut germplasm database, characterization on seed
size and nutrient contents, particularly protein and fat,
therefore, needs to be studied. Sixty peanut germplasm of
Indonesian Legumes and Tuber Crops Research Institute
collection were analyzed their 100-seed weight, ash,
protein, and fat contents at our laboratory. The results
showed that 21 genotypes belonged to large-seeded (≥ 55
g/100 seeds), three small-seeded (< 40 g/100 seeds) and the
rest were medium sizes. Large and medium-seeded is
tailored for boiled, deep-fried, and oven-roasted peanut,
snacks, and confectionary, while small-seeded is favored
for peanut sauce, filler, and flour coated peanuts. The ash
contents slightly varied from 2.53% to 2.98% (dw). Two
genotypes contained protein < 25% (dw) and one genotype
> 30% (dw), while 57 genotypes had protein contents between 25% and 30% (dw). Most genotypes had fat
content between 45% and 50% (dw), two genotypes were
below 45% (dw) and one genotype had considerably high-
fat content (51.23% dw), namely MLGA 0621. This
genotype also belonged to large-seeded (65.2 g/100 seeds),
thus it is promising to be used as a parent material for
further peanut breeding with high-fat content and large
seed size.
Genotype, nutrient values, peanut germplasm
AO-19 The phenology and morphology characteristic of
nine shallot cultivars from TSS in the low land
area, Yogyakarta, Indonesia
Retno Pangestuti1,♥, Endang Sulistyanigsih1, Budiastuti
Kurniasih1, Rudi Hari Murti2, Stephen Harper3, Siti
Subandiyah2 1Faculty of Agriculture, Universitas Gadjah Mada. Jl. Flora No.1,
Bulaksumur, Sleman 55281, Yogyakarta, Indonesia 2Assessment Institute for Agricultural Technology of Central Java. Jl.
Soekarno Hatta Km 26, No 10, Bergas, Kabupaten Semarang 50552,
Central Java, Indonesia 3Departement of Agriculture Forestry and Fisheries, Gatton, Queensland
Australia
The using of TSS (True Seed Shallot) could greatly
improve shallot (Allium cepa L. aggregatum) productivity
in Indonesia. The development and implementation of
"best cultural practices" will be important in the next future. The information on shallot phenology and
morphology characteristic are very useful as basic data for
design any treatment related to timing of cultural practices
(nutrition, pesticide, and plant growth regulator
application) and cultivar selection. The 300 seeds of each
nine shallots cultivar (Tuk Tuk, Lokananta, Sanren, Tropix,
Maserati, Bima, Trisula, Pancasona, and Biru Lancor) were
planted in the screen house of Faculty of Agriculture
Gadjah Mada University, Banguntapan, Bantul District,
Yogyakarta (100 m asl) between May until September
2017. The data collected consists of eight phenology and five morphology characteristics that were obtained by
using descriptive statistics and analysis of ANOVA to
compare difference among means and correlation
coefficients. The research revealed that maximum
vegetative phase did not coincide with bulb initiation
phase. There was two type growth of bulb aggregation
development and. the nine cultivars have variation in
ABS MASY BIODIV INDON, Surakarta, 11-12 November 2019, hal. 67-116 74
aggregation ability. In this study, flowering phase did not
initiate.
Aggregation ability, phenology, true seed shallot
AO-20 Evaluation of flowering of cassava clones: The
effect of genotypes, altitude, paclobutrazol, and
grafting with rootstock Manihot glaziovii
Setyo Dwi Utomo1,♥, Erwin Yuliadi1, Akari Edy1,
Agustiansyah1, Kresna Shifa Usodri2, Fajar Danu
Aslami2, Mustika Adzania Lestari1
Department of Agronomy and Horticulture, Universitas Lampung. Jl.
Sumantri Brodjonegoro No. 1, Bandar Lampung 35145, Lampung,
Indonesia
The objectives of this study were to evaluate the effect of
altitude, genotype, paclobutrazol, and grafting with wild
species on the efficiency of flowering of cassava.
Experiments were conducted in lowland Natar (125 m asl),
South Lampung and highland Sekincau (1000 m asl), West Lampung, Indonesia. Percentage of plants producing
botanical seeds (PPPBS) in Natar (37 clones) at 11 months
after planting 17%; in contrast, PPPBS in Sekincau (32
clones) was 100%. In Natar, PPPBS ranged from 0-100%;
11 out of 37 clones did not produce botanical seeds. At 35
week after planting (WAP), the numbers of male and
female flowers per plants of 6 clones in Sekincau was
higher than those in Natar. Among the 6 clones treated with
500 mg/L paclobutrazol, in Natar only clone BL 2 showed
the number of male flowers significantly higher than
control; in contrast, in Sekincau, Malang 6, Kasetsart Ungu, and BL 2 showed significantly higher. The number
of female flowers per plant (NFFPP) of the 6 clones was
significantly higher in Sekincau than that in Natar; NFFPP
of all clones treated with paclobutrazol 500 mg/L was
higher than that of control. Artificial sexual hybridization
should be feasible in Sekincau. Grafting with M. glaziovii
effectively induce flowering for clone of UJ 3.
Diversity, hybridization, Manihot esculenta Crantz, wild
species
AO-21 Evaluation of crossing local rice with drought
tolerance rice by molecular markers SSR
Aditya Wahyudhi♥, Panjisakti Basunanda
Faculty of Agriculture, Universitas Gadjah Mada. Jl. Flora No.1,
Bulaksumur, Sleman 55281, Yogyakarta, Indonesia
Mentik Wangi and Mentik Susu are local rice originating
from Central Java, Indonesia which have good taste and
unique aroma, and Bluebonnet and Kasalath are drought-tolerant rice. Combining their superior cultivar traits can be
performed using hybridization. SSR markers can be used as
an effective and efficient method for selection. This study
aims to assess the success of the crossing using SSR
molecular markers and to examine the F2 segregation of
the cross between local rice and drought-tolerant rice. This
study was performed in a greenhouse and genetic and plant
breeding laboratory, Faculty of Agriculture, Universitas
Gadjah Mada, Yogyakarta. It used nonfactorial completely
randomized design (CRD). Genotypes used include Mentik
Wangi (MW), Mentik Susu (MS), Bluebonnet (BB) and
Kasalath (KS), F1 and F2. SSR markers used include
RM72, RM228, RM518, and RM20A. Parental polymorphism test using RM72, RM228, RM518, and
RM20A SSR markers showed polymorphism between
parents of local and drought-tolerant rice so that these
markers can be used as markers in F1 and F2 selection.
Evaluation result using SSR showed that the percentage of
heterozygous individuals in Bluebonnet vs Kasalath
(100%), Bluebonnet vs Mentik Wangi (75%), Bluebonnet
vs Mentik Susu (44.44%) and Mentik Wangi vs
Bluebonnet (46.67%) were consistent in every primer used.
Evaluation of F2 showed that the segregation pattern did
not follow Mendel's law of segregation in some crosses due to the small sample size. Differences in F2 heterozygous
individuals in each marker were caused by differences in
the location of the chromosome markers in each SSR
marker used.
Microsatellite, Oryza sativa, polymorphism, segregation
AO-22 Identification of leptin gene in Belgian blue cross
breed cattle
Laksa Ersa Anugratama♥, Tety Hartatik
Department of Animal Breeding and Reproduction, Universitas Gadjah
Mada. Jl. Flora No. 3, Bulaksumur, Sleman 55281, Yogyakarta, Indonesia
Leptin is a gene that affects to animal weight. Leptin gene
control of body weight, feed intake, energy expenditure,
immune function, and reproduction. This study aims to
identify the diversity of the Leptin gene in Belgian Blue
Cross Breed cattle, buffalo, sheep, and goat by comparing
the GenBank Data. The GenBank Data of Leptin was retrieved from NCBI (4 cattle, 1 buffalo, 1 sheep, 3 goats)
and Belgian Blue Cattle Cross Breed sample from Widodo
Makmur Perkasa. Leptin nucleotide sequences were
analyzed using BioEdit to identify SNPs. To create amino
acid change using BioEdit ver. 7.0.5. Phylogenetic tree and
Genetic distance have been analyzed based on the Leptin
gene using MEGA 7.0 program. The result shows that we
found 11 variations. The SNPs were located in an intron (6
SNPs) and exon (5 SNPs). The phylogenetic tree represents
that Bos taurus, Bos indicus, Bos frontalis, Bos grunniens,
Bubalus bubalis are in the same cluster with a close
relationship. The results of this study are expected to provide genetic information that will be used for further
research on the relationship between leptin gene
polymorphisms to animal weight.
Belgian blue cattle, bioinformatics, GenBank, Leptin gene,
ABS SOC INDON BIODIV, Surakarta, 11-12 November 2019, pp. 67-116 75
livestock
AP-01 Genetic diversity of low risk Stelechocarpus
burahol from Magelang and Purworejo (Central
Java, Indonesia) revealed by ISSRs
Tri Suwarni Wahyudiningsih♥, Esna Dilli Novianto,
Erry Purnomo, Dian Sartika 1Department of Agrotechnology, Universitas Tidar. Jl. Kapten Suparman
No. 39, Kota Magelang 56116, Central Java, Indonesia. 2Genetica and Improvement Laboratory, Faculty of Biology, Universitas
Gadjah Mada. Jl. Teknika Selatan, Sekip Utara, Sleman 55281,
Yogyakarta, Indonesia
Stelechocarpus burahol (Bl.) Hook.F & Th. as
multipurpose tree is useful as local fruit and as a raw
material for medicine, so it needs tree breeding strategy
research. The aim of this study was to determine Genetic diversity of S. burahol from Magelang and Purworejo
revealed by ISSRs. This study revealed that ISSR markers
are helpful for evaluating A as well as for evaluating intra-
species genetic variations. Molecular analysis of genotype
character was conducted using four ISSR primers. A total
of 47 bands, of which 39 (82.97%) were polymorphic. The
maximum number of DNA bands was generated by UBC
873 primer (14 bands) while minimum number of DNA
bands was produced by UBC 854 primer (9 bands). Every
primer was produced different patterns of DNA band,
range from 150-1500 bp. Primers of UBC-812, UBC-847-Y, UBC 854, and UBC 873 show polymorphisms ≥ 50.
The high overall polymorphism of 83.532% indicates that
twenty samples of S. burahol have their own genotypic
characters which are expressed through phenotypic
characters. Therefore, genetic diversity of S. burahol has
enriched Indonesian germplasm which will greatly
contribute to worldwide S. burahol plant improvement.
genotypic characters, molecular characters, PCR-ISSRs,
Stelechocarpus burahol
AP-02 Seed coating plus biological agents to increase of
rice growth for blast disease in seedling
Tantri Palupi♥, Fadjar Riyanto
Faculty of Agriculture, Universitas Tanjungpura. Jl. Achmad Yani,
Pontianak 78124, West Kalimantan, Indonesia
The research that consisted of two activities, was
conducted from March to November 2018 at the Plant Pest
and Disease Laboratory and Experimental Garden on the Faculty of Agriculture, Tanjungpura University, Pontianak,
Indonesia. Experiment 1, the aim to obtain five biological
agents that are compatible to one another. Experiments 2 to
determine the effect of rice seeds coated with biological
agents to evaluate the effectiveness of seed coating on the
incidence rate of blast disease and seedling growth of rice
in the screen house. In the first experiment, five isolates of
bacterial antagonists, i.e: isolates SP21, SP31, RP21, TP12,
and TP11, were tested for their compatibility with each
other on PSA plates. In the second experiment used a
completely randomized design with a single factor (seed
coating) consisting of seven levels, namely: seed coating
enriched SP21+SP31 isolates; seed coating enriched
isolates SP21+RP21; seed coating enriched isolates
SP21+TP12; seed coating enriched isolates SP31+RP21;
seed coating enriched isolates RP21+TP11; fungicides; and positive control (rice seeds contaminated with blast).
Observations were made on the incidence rate of blast
disease and plant height at 2, 3, and 4 weeks after planting.
The results of experiment 1 showed that isolates
SP21+SP3, SP21+RP21, SP21+TP12, SP31+RP21, and
RP21+TP11 had good growth compatibility with no
antagonism. Therefore, there are isolates were selected and
used as biocontrol agents in further studies. Results of
Experiment 2 showed that treatment with seed coating +
isolates RP21+TP11 no symptoms of blast attack at all
until observation 4 weeks after planting, with better growth compared to positive control treatments.
blast disease, coated seeds, seed quality, Pyricularia
grisea, seedling growth
AP-03 Analysis of lactate dehydrogenase gene expression
as an adaptation strategy for jambal catfish to
stress the aquatic environment
Sularto1, Lies Emmawati Hadie1,♥, Huriah Marnis2,
Jadmiko Darmawan2, R.R. Sri Puji Sinarni Dewi3 1Fish Breeding Research Insitute. Jl. Sukamandi No. 2, Subang 41263,
West Java, Indonesia 2Research Centre for Aquaculture. Jl. Ragunan 20, Pasar Minggu, South
Jakarta 12540, Indonesia 3Freshwater Aquaculture Research Center and Fisheries Counseling. Jl.
Sempur 1, Bogor 16121, West Java, Indonesia
The main element in the aerobic animal body cell
metabolism is oxygen. The metabolic process is affected by
fluctuations in oxygen solubility. If there is an acute lack of
oxygen, it will cause adverse physiological changes in the
body. The purpose of this study was to analyze the
expression of the lactate dehydrogenase gene in jambal
catfishes (Pangasius djambal Bleeker, 1846) to stress the
aquatic environment. Jambal catfish measuring 24.21 + 0.96 cm with a weight of 223.42 + 31.23 g were used as
test fish in this study. Low oxygen levels (hypoxia), high
oxygen (normoxia), and naturally high fluctuating
conditions as control were used as treatments.Variables
observed included: gill filament area, and lactate
dehydrogenase gene expression. LDH gene expression is
observed through the liver and pituitary. LDH gene
expression analysis was used Reverse Transcriptase
Polymerase Chain Reaction (RT-PCR) method. The r