Interphase Cells: Are they in G1, S or G2?

TIME FOR MITOSIS LAB

In this lab, you will determine the amount of time a cell spends in each phase of mitosis and interphase by counting the number of cells in prophase, metaphase , anaphase and telophase.

Let’s review the Cell Cycle: Interphase and M phase…..

Cells from Whitefish blastula undergoing mitosis. Can you identify interphase, prophase, metaphase, anaphase and telophase?

THE CELL CYCLE

Cell Cycle Animation

What do mitotic onion root tip cells look like?

Oogenesis – process that makes the sex cell, oocyte, has unequal cytokinesisSpermatogenesis – process that makes the sex cell, spermatocyte, has equal cytokinesis

Before beginning the lab, let’s watch the animation of Mitosis and

Cytokinesis Mitosis and Cytokinesis

As you watch the animation, look for when chromatin, chromosomes and sister chromatids are present.

As you watch the animation, note what happens to the cell in each phase.

As you watch the animation, can you describe what is happening to the amount of genetic material.

Let’s do the lab…. Below is only (1) of the (4) sample cards that you will be given. You must count all the cell on the cards, note the number on the table in the lab. Then identify cells in a phase of mitosis and write that number on the table, too

The Results: Table indicates the # of and % of cells in each phase

Phase Group 1 Group 2 Group 3

Prophase 70/485 (14.4%)

118/371 (31.8%)

41/530(7.7%)

Metaphase 12/485 (2.5%)

12/371(3.2%)

22/530(4.2%)

Anaphase 9/485 (1.9%)

4/371(1.0%)

13/530(2.5%)

Telophase 7/485(1.5%)

19/371(5.1%)

29/530(5.5%)

After completing the lab…

Be sure that all data is recorded on the data table.

Complete any calculations on the data table.

Answer all lab questions on the lab. Do the following:

If the total time for onion root tip cell cycle is 1,440 minutes, determine the amount of time each cell spends in prophase, metaphase, anaphase and telophase using the data from the data table.

How much time is spent in M phase and Interphase?

PHASES OF MITOSIS

If you were absent or want to review the “Determining the Time for Mitosis” lab, you can use the link below to perform the lab on this site.