Interview Questions - Set 2

7/30/2019 Interview Questions - Set 2

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Choose the correct option. / Fill in the blanks.

Question Ans 1 Ans 2 Ans 3 Ans 4 Ans 5 CorrectAnswer

Which enzyme is incharge ofsynthesizing themajority of theribosomal RNA?

DNA Polymerase RNA Polymerase I RNA Polymerase II Ribosome Polymerase 3

Which of thefollowing is NOT a

type of spontaneousmutation?

Tautomerism Depurination Slipped strand mispairing Base analog guidedmutation

4

Mis-sense mutationsare the ones which:

Code for a differentamino acid than theoriginal one.

Code for the sameamino acid as theoriginal one.

Code for same amino acidas the original one butwith a different function.

Code for a stop and cantruncate the protein.

1

Which of thefollowing is not a"wobble base pair"?

Guanine-Uracil Inosine-Uracil Inosine-Adenine Inosine-Guanine 4

In which of thefollowing DNAregions does thetranscriptioninitiation take place?

Operator Promoter Regulator Initiator 2

Which gene normally

codes for regulatoryproteins controllingcell growth and celldifferentiation?

Growth gene Proto-oncogene Hormone gene Immunoglobin gene 2

What are DNA,consisting of verylarge arrays ofcyclically repeating,non-coding DNAfragments known as?

Euchromatin Heterochromatin Telomere Satellite DNA 4

Which of thefollowing processescan be used todeterminetranscriptioninitiation site?

Foot Printing Northern Blotting Primer Extension Nick Translation 3

Which of thefollowing is notrequired whilemaking DNA probesthrough the Nick

Translation process?

DNA Polymerase DNase Deoxyribonucleotides Primers 4

Which of thefollowing techniquesdid Messelson andStahl use to showthat DNA replicationis semi conservativein nature?

Radioactivity Centrifugation Chromatography Dialysis 2

In Griffith'sexperiment, which of

the following areINCORRECT withrespect toStreptococcuspneumoniae for theprocess oftransformation?

The extract ofbacteria is treated

with lipases,proteases and RNasedid not jhave anyeffect on the abilityto infect the mouse.

The live R strain ofStreptococcus

pneumoniae waspotentially ineffectiveto infect the mouse.

The dead S strain ofStreptococcus pneumoniae

was potentially ineffectiveto infect the mouse evenwhen mixed with live Rstrain.

The extract ofStreptococcus

pneumoniae having liveR starin and dead Sstrain could infect themouse.

2

Which of thefollowing enzymes isproduced by lacoperon?

Beta-lactamase Alpha-amylase Beta-galactosidase Alpha-galactosidase 3

Which of thefollowing virusesdoes not have DNA

Tobacco MosaicVirus

T2 bacteriophage Small pox virus Herpes virus 1

INTERVIEW QUESTIONS - SET 2

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as genetic material?

Which of thefollowingchromosomes is aspecial type ofchromosome foundin amphibians, birdsand insects but notfound in mammals?

Lampbrushchromosome

Adrenal glandchromosome

Micro chromosome Lampbeadchromosome

1

What is the correctsequence of stagesof cell division inmeiosis?

Pachytene,Diplotene,Diakinesis,Leptotene, Zygotene

Leptotene, Zygotene,Pachytene,Diplotene, Diakinesis

Diplotene, Leptotene,Zygotene, Pachytene,Diakinesis

Diplotene, Leptotene,Diakinesis, Pachytene,Zygotene

2

Which of thefollowing enzymescauses negativesuperhelical turnsduring DNAreplication?

DNA Gyrase DNA Polymerase Helicase DNA Primase 1

Which of thefollowing is true forthe parts of DNAdouble helix that

tend to separateeasily?

They have high GCcontent and high ATcontent.

They have high GCcontent and low ATcontent.

They have low GC contentand high AT content.

They have low GCcontent and low ATcontent.

3

Which of thefollowing parts ofmessenger RNA hasthe start codon?

Leader sequence Trailer sequence Reading frame Promoter region 3

Which of thefollowing proteins isinvolved in folding ofProkaryotic genome?

Protein SR Protein HU Polymerase Helicase 2

With which of thefollowing enzymesdoes primase form acomplex in order toassist in DNAreplication?

DNA Helicase DNA Polymerase DNA Ligase DNA Nuclease 1

Which of thefollowing causestranscription to stop?

When RNAPolymerase meets astop codon.

When DNAPolymerase meets astop codon.

When DNA Polymerasemeets a terminatorsequence.

When Taq Polymerasemeets a stop codon.

4

Which of thefollowing enzymes isa part of primosomecomplex that causesDNA replication?

Ligase DNA Polymerase Helicase Nuclease 3

Which of thefollowing enzymescleaves the RNAprimer from the DNAmolecule duringDNA replication?

RNase H RNase A DNA Polymerase II DNA Polymerase I 4

Which of thefollowing is true forDNA replication?

It takes place in 5'-3'direction and is semiconservative innature.

It takes place in 5'-3'direction and isconservative innature.

It takes place in 3'-5'direction and isconservative in nature.

It takes place in 3'-5'direction and is semiconservative in nature.

1

Proof reading is a/an_______.

DNA Gyrase activity 3'-5' Exonucleaseactivity

5'-3' Exonuclease activity Endonuclease activity 2

Which of thefollowing is a featureof homologousgeneticrecombination?

Holliday intermediate Klenow fragments Transposition Replisome 1


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Which of the

following proteinsactivatestranscription in E.Coli?

cAMP receptor

protein

Transcription factor Humulin ATP-binding proteins 1

Which of thefollowing bases is

not found in RNAgenetic code?

Adenine Guanine Thymine Cytocine 3

Which of thefollowing maximizesthe number ofcodons that can beread by a particulartRNA molecule?

Inosine Xanthosine Thymidine Guanosine 1

Upstream Activation

Sequence (UAS) inyeast is also knownas _______.

Adaptor Enhancer Promoter Activator 2

Which of thefollowing groups inGuanine is

responsible forGuanine not beingable to form adouble bond withAdenine?

NH2 OH CH3 SH 1

What is the functionof photolyases?

It cleavesphosphodiesterbonds at the end of apolynucleotide chain.

It bindscomplementary DNAstrands and breakscertain types ofpyrimidine dimers.

It cleaves phosphodiesterbonds within apolynucleotide chain.

It repairs single-stranded discontinuitiesin double strandedDNA molecules.

2

Which of thefollowing enzymes isused to join DNAfragments together?

Nucleases Kinases Ligases DNA Polymerases 3

Which of thefollowing groups ofenzymes areresponsible for baseexcision repair in E.Coli, a mechanism

by which damagednucleotides in DNAare removed andreplaced?

DNA Glycosylase, APEndonuclease, DNALigase and DNAPolymerase.

AP Endonuclease,Photolyase, DNALigase and DNAPolymerase.

DNA Glycosylase, Kinase,DNA Ligase and DNAPolymerase.

Kinase, Photolyase,DNA Ligase and DNAPolymerase.

1

The lac repressorprotein is a

________.

Dimer Trimer Tetramer Hexamer 3

Which of thefollowing is a featureof 'Restriction

enzyme'?

(i) It cuts doublestranded DNA atspecific sites.(ii) It cuts themethylated DNA.(iii) It makes twoincisions, one oneach strand, in thesugar-phosphateback bone.

(i) and (ii) (i) and (iii) (ii) and (iii) (i), (ii) and (iii) 2

Beginning with 30template DNAmolecules, howmany amplicons willbe produced after 25

2x1010 molecules 1x109 molecules 1x1010 molecules 2x109 molecules 2


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cycles of Polymerase

Chain Reaction?

What are homingendonucleases?

They are DNaseencoded by intronsor inteins.

They are DNaseencoded by exons.

They are DNase found inbacteria.

They are DNAPolymerases.

1

Which of thefollowing is false for

restrictionendonucleases?

They are found inEukaryotes.

They were developedas defense

mechanism againstinvading viruses.

HindII was the firstrestriction endonuclease to

be isolated.

They do not requireany other enzyme

activity to cut thestrands of DNA.

4

Which of thefollowing enzymescuts the 3' hairpinloop during theconstruction ofcDNA?

S1 Nuclease DNA Polymerase RNA Polymerase Ribonuclease 1

Transformation doesnot work well forlinear DNA because:

DNA does not getintegrated into themain chromosome.

Exonuclease insidethe bacteria rapidlydegrades the DNA.

DNA gets degraded byheating the step at42C.

DNA is cleaved by DNAPolymerases.

2

Which of thefollowing principlespermits heating andcooling of blocks by

reversing the electriccurrent in a thermalcycler (PCRmachine)?

Chemiluminescence Peltier effect Doppler effect Electromagnetic effect 2

Which of thefollowing is an ionicdetergent used inPolymerase ChainReaction whichprevents formationof secondarystructure?

Tween-20 Dimethyl Sulfoxide(DMSO)

NonylPhenoxypolyethoxylethanol(NP-40)

Triton-x 100 2

Which of thefollowing classes ofrestriction enzymescleaves methylatedDNA?

Type-I Type-II Type-III Type-IV 4

Taq Polymerase usedin Polymerase ChainReaction is a heatstable _______.

RNA Polymerase DNA Polymerase mRNA Polymerase tRNA Polymerase 2

Which of thefollowing moleculesdoes pyrosequencingoperate on?

Protein DNA RNA Lipid 2

Which of thefollowing is best forDNA storage?

Normal saline Tris EDTA Distilled water Acetic acid 2

SmaI is a restrictionenzyme that

produces ______.

Blunt ends only Sticky ends only Both blunt and sticky ends Depends upon themolecule being cleaved.

1

Which of thefollowing is true for

Type I restrictionenzyme?

It has ligase activity. It has methyltransferase activityonly.

It has both restriction andmethyl transferase activity.

It has both ligase andmethyl transferaseactivity.

3

Which of thefollowing is true forM13 bacteriophageDNA replication?

It uses the negativestrand of ReplicatingForm (RF) of thephage as template toform new strands.

It uses the positivestrand of ReplicatingForm (RF) of thephage as template toform new strands.

The phage uses its ownproteins to synthesize thenew strands.

It can use eitherpositive or negativestrand of theReplicating Form (RF)of the phage astemplate to form newstrands.

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How many fold

amplifications wouldhappen if a targetsequence, whoseflanking sequence isunknown, undergoes5 cycles in PCR?

25 fold 125 fold 3125 fold 0 fold 4

According to theMenedelism, a typicalmonohybrid crossshows thephenotypic ratio of3:1. Whatpercentage of theoffsprings will behaving Bb genotypesif both of the parentsare having Bbgenotypes in amonohybrid cross?

0.25 1 0.5 0.75 3

What is a sepiasomiccondition?

It is a decrease inchromosomenumber (2n-2).

It is an increase inchromosome number(2n+5).

It is an increase inchromosome number (3n).

It is a decrease inchromosome number(2n-1).

2

Which of thefollowing has lost itsprotein codingability?

Pseudogenes Introns Exons Inteins 1

Which of thefollowing types ofgenome annotationsare ORFs related to?

Functionalannotation

Physical annotation Chemical annotation Structural annotation 4

What is percentrecombination in atest cross equal to?

Double cross over Single cross over Map-distance Map area 3

Mutation in SNC Agene that causesParkinson Diseasehas been mapped at

____________.

Chromosome 7 Chromosome 4 Chromosome 5 Chromosome 1 2

Which of thefollowing diseasesillustrates Mendel's

law?

Fragile X syndrome Huntington's disease Arthritis Sickle cell anemia 4

The differences ingenome sizes withina class of organismsare almost entirelydue to differences inthe amount of

_______.

Protein coding DNA Non protein codingDNA

Protein coding RNA Non protein codingRNA

2

Which of thefollowing enzymes

finds and removes

RNA primers duringDNA replication?

RNA Primase RNA Polymerase DNA Polymerase epsilon DNA Polymerase I 4

In direct reversalDNA repairmechanism, whattype of abnormalbond betweenadjacent pyrimidinebases is the result offormation ofpyrimidine dimersupon irradiation withUV light?

Phosphodiester bond Covalent bond Peptide bond Glycoside bond 2


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Which one of the

following is the moststable?

DNA-DNA Duplexes mRNA-rRNA

Duplexes

RNA-DNA Hybrid mRNA-tRNA Hybrid 3

Which of thefollowing paths doesthe 'proteintranslation from

mRNA' take?

N-terminus toC-terminus

C-terminus toN-terminus

N-terminus to N-terminus C-terminus toC-terminus

1

_____________ is adiverse group oflong primarytranscripts formed inthe eukaryoticnucleus, many ofwhich will beprocessed to mRNAmolecules by

splicing.

Heterogeneousnuclear RNA

Homogeneousnuclear RNA

Large nuclear RNA Small nuclear RNA 1

In genetictranscription, whichshort DNA sequenceis the most commontype of core

promoter ineukaryotes?

Transcription FactorII B

Transcription FactorII D

TATA Box Template Strand 3

Which of thefollowing anticodonsdoes the tRNAshave, that arecomplementary tothe codon in mRNAand can be chargedcovalently withamino acids?

3' terminal UGC ends 3' terminal AAU ends 3' terminal UGU ends 3' terminal CCA ends 4

Which of thefollowing mutationscauses Tay-Sachsdisease?

Point Mutation Missense Mutation Nonsense Mutation Frameshift Mutation 4

Which one of thesemethods can be usedfor analyzing thepattern ofmethylation in aspecific region ofDNA?

Methylation-specificPCR (MSP)

Pyrosequencing Southern Blotting Bisulfite Mapping 4

What do missensemutations code for?

Amino acid thatcodes for stop signal

Different amino acid Same amino acid Amino acid that codefor a change

2

Which type ofrestriction enzymestarget methylatedDNA?

Type I Type II Type III Type IV 4

In Cos sequences,

which site holds the

terminase while it isnicking andseparating thestrands?

Cos B Cos N Cos Q Cos X 1

What is a terminalDeoxynucleotidyl

Transfrerase?

A nuclease A ligase A polymerase A lipase 3

Which of thefollowing is SOLiDsequencingspecifically used for?

dNTPs ddNTPs Oligonucleotides Oligonucleosides 3


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Which technique is

used for producingtemplates forpyrosequencing?

Solid Phase

TemplatePreparation

Liquid Phase and

Enzymatic TemplatePreparation

Enzymatic Template

Preparation

Liquid Phase Template

Preparation

Solid Phase and

Enzymatic TemplatePreparation

5

Epistatis can becontrasted withdominance, which is

an interactionbetween alleles atthe:

Same gene locus Different gene locus Same allele locus Different allele locus 1

Inheritance of skincolour in humanbeings is an exampleof:

Sex linkedinheritance

Quantitativeinheritance

Sex limited inheritance Qualitative inheritance 2

In which phase ofcell cycle can

euchromatin andheterochromatin bedistinguished?

Interphase Metaphase Prophase Anaphase 1

Which of thefollowing is aconsequence of

overlapping genes?

Increased thetolerance formutation.

Reduced thetolerance formutation.

Trisomy Tetrasomy. 2

Turner syndrome isan example ofmonosomy where an

individual is bornwith:

X Chromosome Y Chromosome XXX Chromosome XXY Chromosome 1

Which of thefollowing is not acause of damage tobases of DNA?

Deamination Depurination Depyramidination Depolymerization 4

Which of thefollowing containsAT richAutonomouslyReplicatingSequences (ARS)?

Bacterial genome Fungal Genome Yeast genome Viral genome 3

In which of thefollowing forms doesDNA exists at arelative humidity of70%?

A-form B-form C-form Z-form 1

Which of thefollowing operonsrequire CataboliteActivator Protein(CAP) forrepression?(i) Lac operon(ii) Trp operon(iv) Arabinose

operon.

Both (ii) and (iii) (i) only (ii) only Both (i) and (iii) 4

Which of thefollowing RNApolymerase helps intranscription oft-RNA and smallRNA?

RNA Pol-I RNA Pol-II RNA Pol-III None of above 3

Which of thefollowing antibioticsinhibits thefunctioning ofprokaryoticribosomes?

Aminoglycosides Chloramphenicol Tetracycline Cycloheximide 1


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The ____________

have long terminalrepeats (LTR's)similar toretroviruses.

V iral Transposons Non-Viral

transposons

DNA transposons RNA transposons 1

Which of thefollowing is not an

intercalating agent?

Ethidium Bromide Daunomycin Doxorubicin Tris Chloride 4

During the synthesisof cDNA, 3'-OH isprovided byformation of a loopby the singlestranded DNA itself.

This loop is furtherreleased by

___________.

EcoRI S1 nuclease RNA polymerase Helicase 2

Which of thefollowing plasmidscontains partitiongenes that promotethe even distributionof plasmids after

bacterial celldivision?

F-plasmid Rec-plasmid GFP-plasmid Col-plasmids 1

Which of thefollowing polymeraseis produced instationery phase andconsidered to be anerror-pronepolymerase?

DNA Polymerase-I DNA Polymerase-II DNA Polymerase-III DNA Polymerase-IV 4

The process ofsequencing of wholegenome in which theDNA is broken intopieces, sequencedand finally, the readsare overlapped andcorrect reads areobtained is known as

___________.

Pyrosequencing Shot-gun method of sequencing

Chromosome walking Dideoxy method of sequencing

2

Which of thefollowing moleculesis not utilized &nbspin pyrosequencingtechnique?

DNA Polymerase Luciferase Apyrase Dideoxynucleotides 4

Which of thefollowing is not asubstrate forglucuronidase?

5-bromo-4-chloro-3-indolyl-beta-D-glucuronic acid(X-Gluc)

p-nitrophenyl-beta-D-glucuronide

4-methylumbelliferyl-beta-D-glucuronide (MUG)

Isopropyl-beta-D-1-thiogalactopyranoside(IPTG)

4

Which of thefollowing is anapplication of cDNAlibraries?

Mendelian genetics Reverse genetics Entomology Inheritance studies 2

When conjugation isinitiated by a signal

_______ creates anick in one of thestrands of theconjugative plasmidat the oriT and helpsin transfer of DNA.

Helicase Relaxase Endonuclease Exonuclease 2

In which of thefollowing phasesdoes independentassortment of genesoccur in eukaryotes?

Metaphase-I Anaphase Prophase Telophase 1


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What do the densely

stained and lightlystained areas of anucleus denoteduring the process ofnuclear staining?

Heterochromatin and

Euchromatinrespectively

Euchromatin and

Nucleolusrespectively

Heterochromatin and

Cytoplasm respectively

Cytoplasm and

Nucleolus respectively

1

Which of the

following is true?(A) C rossing overhappens betweentwo non-sisterChromatids.(B) Thecomplementarity ofthe strands aftercrossing over is notpreserved.(C) Holliday Junctionis the trihedralstructure associatedwith crossing over.(D) Crossing overtakes place onlybetween

homologouschromosomes.

Both A and D A,B and C Both B and D B, C and D Both A and C 1

Which of thefollowing is NOT a

DNA damage?

Thymine dimers Pyrimidine dimers Cyclobutane PyrimidineDimers

UV-endonuclease-sensitive-sites

3

Transposition is alsocalled illegimaterecombinationbecause

________________.

It require homologybetween sequences.

It helps in formingnew recombinants.

It is not site specific. It results into death ofthe cell.

3

The meltingtemperature or Tm ishighest for thoseDNAs which containhighest proportion of

______________.

C+A A+T C+G A+T 3

Zinc finger motifcomprises two betastrands and onealpha helical region.Which of these is/areresponsible forinteraction with theDNA?

Beta strands One beta strand andthe alpha helicalregion

The alpha helical region Both the beta strandsand the alpha helixtogether

3

Which of thefollowing requires

TA TA BindingProtein (TBP) forproper initiation oftranscription?

RNA polymerase I RNA polymerase II RNA polymerase III All of the above 3

The relative distancebetween two genescan be calculated bytaking the offspringof an organismshowing two linkedgenetic traits, andfinding thepercentage of theoffsprings where thetwo traits do not runtogether. If thepercentage of thedescendants thatdoes not show bothtraits are higher it

The two genes arecloser to each otheron the chromosome.

The two genes arefar apart to eachother on thechromosome.

The two genes areoverlapping on each otheron the chromosome.

The two genes do notbelong to the samechromosome.

2


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means that:

Bacteriophages carryportions of DNAfrom one bacteria toanother in a processcalled:

Transformation Transfection Transduction Conjugation 3

Which of the

following is theinitiation codon?

AUG CUG AUC AGU 1

The complementaryDNA strand for5'-TACGATCATAT-3'would be:

3'-TA CGA TCA TA T-5' 3'-A TGCTA GTA TA -5' 3'-A UGCUA GUA UA -5' 3'-GCA TA TA CGCG-5' 3'-TA TA CTA GCA T-5' 2

Which of thefollowing is not truefor house keepinggenes?

Constitutiveeuchromatin that isalways turned on.

Constitutiveheterochromatin thatis always turned on.

Constitutive euchromatinthat is always turned off.

Constitutiveheterochromatin that isalways turned off.

1

The first amino acidincorporated in aprotein chain ofeukaryotes andprokaryotes is:

Histidine Alanine Cysteine Methionine 4

How does the stranddirected mismatchrepair systemdistinguish the newDNA strand from theold one?

Hydroxyl groups areadded to all Aresidues in thesequence GATCsome time after theA has beenincorporated into anewly synthesizedDNA chain.

Methyl groups areadded to all Aresidues in thesequence GATCsome time after theA has beenincorporated into anewly synthesizedDNA chain.

Phosphate groups areadded to all A residues inthe sequence GATC sometime after the A has beenincorporated into a newlysynthesized DNA chain.

Ethyl groups are addedto all A residues in thesequence GATC sometime after the A hasbeen incorporated intoa newly synthesizedDNA chain.

2

What is the function

of DNAtopoisomerasesduring DNAreplication?

They catalyze

nucleosidetriphosphatepolymerization.

They help in opening

up the DNA helix sothat it can be copied.

They seal together the

discontinuouslysynthesized lagging-strandDNA fragments.

They help to relieve

helical winding and toprevent DNA tangling.

4

Which of the

following is not acomponent of anucleosome?

DNA segment Histone protein Chromatin Ribosomes 3

What is the role ofthe cAMP-CAPcomplex in E. coli?

It binds to theoperator, inhibitingtranscriptioninitiation.

It binds to the CAPsite, where itinteracts with RNApolymerase to inhibittranscriptioninitiation.

It binds to the operator tostimulate the rate oftranscription initiation.

It binds to the CAP site,where it interacts withRNA polymerase tostimulate the rate oftranscription initiation.

4

The mechanism ofpre-mRNA splicingimplies that thesplicing machinerymust recognize

_______ of theprecursor RNAmolecule.

the 5' splice site andthe 3' splice site

the 5' splice site andthe branch point

the 3' splice site and thebranch point

the 5' splice site, the 3'splice site and thebranch point

4

Which of thefollowing statementsis true regardingeukaryotic mRNA?

Eukaryotic mRNA&nbsp is essentiallymature upontranscription and

requires noprocessing.

Eukaryotic translationis coupled totranscription andoccurs

co-transcriptionally.

Introns are &nbsp presentin the mRNA of alleukaryotes.

Both ends of eukaryoticmRNAs are modified bycapping on the 5' endand by polyadenylation

of the 3' end.

4

Which of thefollowing statementsis false regardingcodons?

Most amino acids arerepresented by morethan one codon.

Codons for the sameamino acid tend tocontain the samenucleotides at thefirst and secondpositions.

Three codons act asinitiation sites signaling thestart of the protein-codingsequence.

The tRNA moleculebinds to the codonduring translation.

3


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The two subunits of

the ribosome jointogether on the

________ , usuallynear its _________end, to ini tiate thesynthesis of aprotein.

mRNA molecule, 5' rRNA molecule, 5' mRNA molecule, 3' rRNA molecule, 3' 1

How doestetracycline inhibi tthe protein synthesisin bacteria?

It binds in the exitchannel of theribosome andthereby inhibitselongation of thepeptide chain.

It blocks the peptidyltransferase reactionon ribosomes.

It blocks binding ofaminoacyl-tRNA to A -siteof ribosome.

It blocks thetranslocation reactionon ribosomes.

3

Which of thefollowing statementsis false regarding the

use of transposableelements in geneticresearch?

They are naturallyoccurring mutagens.

They function asDNA mismatch repairsystems.

They act as cloning tags. They function asvehicles for introducingforeign DNA into model

organisms.

2

Insertions ordeletions withincoding regions may

lead to___________ andprematureterminationof___________.

Missense mutation;transcription

Frameshift mutation;translation

Missense mutation;translation

Frameshift mutation;transcription

2

What feature(s)is/are required for aDNA molecule toserve as a vector?(i) It must be able toindependentlyreplicate itself andthe fragment itcarries.(ii) It should carry aselectable marker

gene.(iii) It should containseveral recognitionsequences.(iv) It should be easy

to recover from thehost cell.

(i), (ii) and (iv) (ii), (iii), and (iv) Both (i) and (iii) Both (i) and (ii) All - (i), (ii), (iii) and(iv)

5

pUC18 plasmidcarries a fragment ofthe bacterial lacZgene, and thepolylinker is insertedinto this fragment. Ifa DNA fragment isinserted into thepolylinker, the

bacterial cell carryingthe plasmid forms________ colonieswhen grown onmedium containing

______ .

Blue, X-Gal White, X-Gal Blue, X-Lac White, X-Lac 2

Which of thefollowing statementsis true aboutcosmids?

They are also calledphagemids.

They can carry DNAinserts up to 15 kb inlength.

They are plasmid vectors. They contain asequence of phagelambda.

4

What is a knockoutmouse?

It contains multiplecopies of a humangene.

It lacks a knownnormal gene.

It contains one normal andone mutant copy of aparticular gene.

It contains a genewhich is over expressed

2


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How can a specific

clone be identified ina DNA library?

By gene targeting. By colony

hybridization.

By recombinant DNA

technology.

By DNA hybridization. 2

___________ is theprocess by which ahost organism cantake up DNA from its

environment. E. colican take up DNAafter treatment with

_________.

Transformation,sodium ions

Transformation,calcium ions

Transvection, sodium ions Transvection, calciumions

2

What is theprobability that aplant of genotypeAaBb will beproduced fromparental plants of the

genotypes AaBb andAabb if the two genepairs independentlyassort during gameteformation?

0.5 0.25 0.125 1 2

The following are

frequencies ofcrossing overbetween each pair ofthree genes: A and B0.5 %; B and C 34.5%, A and C 35.4 %.What is the order ofthe genes on thechromosome?

A-B-C B-A-C A-C-B B-C-A 1

How many possiblereading frames mustbe examined insearching for ORFs(Open ReadingFrames) in anunidentified DNA

sequence?

1 2 3 6 4

Which of thefollowing convertssingle stranded DNAto double strandedDNA during cDNAsynthesis?

DNA Polymerase Nuclease RNase Reverse Transcriptase 1

Infection of the samecell by two viruses,in which oneprovides a geneproduct which theother requires iscalled ___________.

Anabolism Canabolism Complementation Symbiosis 3

The endonucleases

which cut DNAmolecules at a &nbspparticular pointwithin a specificregion of six basepairs are called

______________.

Site specific

nucleases

Sequence specific

nucleases

Base specific Amino acid specific 2

The cofactorrequired for E.coliDNA ligase is:

NAD+ ATP NADPH AMP 2

The best example forprocessive enzyme inprokaryote DNAsynthesis is:

DNA Polymerase III DNA Polymerase IV DNA Polymerase II DNA Polymerase I 1


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A fragment of

synthetic DNAcontaining arestriction site thatmay be used forcloning of genes iscalled:

Adaptors Linkers Regulator Homopolymeric tail 2

If a mutation is fixedin the population,those genes arecalled:

Unitary pseudogenes Duplicated genes Processed genes Overlapping genes 1

__________ alterthe specificity of&nbsp RNApolymerase for agiven promoter inprokaryotic

transcription.

Gamma factors Sigma factors Delta factors Alpha factors 2

Enhancement ofinteraction betweenRNA polymerase & aparticular promoteris done by:

Activators Enhancers Repressors General Transcriptionfactors

1

The large fraction ofthe C-value ineukaryotic cell ismade by:

Tranposons Insertinal elements Nucleases Mobile genetic elements 1

The recombinantfrequency of 1% isequivalent to:

1 centimorgan 10 centimorgans 5 centimorgans 100 centimorgans 1

A genetic map is amap based on thefrequencies ofrecombinationbetween markersduring:

Meiosis Mitosis Crossing over Replication 1

___________disease is anexample forMendelian trait.

Mulberry HeartDisease

Sickle-cell anemia Xerophthalmia Scurvy 2

Mating the F1 hybrid

with one of theparent is called

__________.

Test cross Back cross Three-point cross Two-point cross 2

Misalignment ofbases in DNAreplication is notcaused by:

Acridine molecules Alkylating agents Base analogs Nitrous acid 1

Mueller inducedlethal mutation inDrosophila by

treating with:

Gamma rays X-rays IR rays Alpha rays 2

Maxam-Gilbertmethod involves

_________modification of DNA .

Chemical Enzymatic Radioactive Flourescent 1

In which of thefollowing sequencingmethodreversible-dyeterminators areused?

Solid Illumina Ion semiconductor Pyrosequencing 2

In which of thefollowing methods ofsequencing theenzyme luciferase is

Automatedsequencing

Pyrosequencing Sangers dideoxy method Maxam-Gilbert method 2


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used?

In which of thefollowing sequencingmethods thetechnique ofchromosome walkingis observed?

Shotgun Sequencing Maxam-Gilbertmethod

Automated sequencing Sangers dideoxymethod

1

The reporter gene,which causes cellsthat express it toglow green underblue light, is called:

GUS GFP Luciferin YFP 2

A tRNA attached toits amino acid iscalled

_____________.

Uncharged tRNA Charged tRNA Neutralized tRNA Close ended tRNA 2

Which amino aciddoes the IUBMBsymbol "L" indicate?

Phenylalanine Histidine Leucine Lysine 3

The enzyme involvedin the attachment ofcorrect amino acid to

each type of tRNA is___________.

Aminoacyl-tRNAsynthetase

DNA Polymerase RNA Polymerase Aminoacyl-tRNA ligase 1

Which of thefollowing is stopcodon amber?

UAA UGA UAG UGG 3

Which of thefollowing is a featureof Prokaryoticgenome?

It is usually circularand tangled duringthe cell growthphase.

It is found in thenucleoid region.

It is highly extended andtangled during celldivision.

It is compressed andfolded.

3

What is a ribozyme? RNA without sugarwhich is foundprokaryoticorganisms.

RNA with extraphosphate.

RNA with enzymaticproperty.

RNA with catalyticproperty which is foundonly in the nucleus ofeukaryotic organisms.

3

Which of the

following reactions isNOT a posttranslationalmodification?

Hydroxylation Glycosylation Phosphorylation Polyadenylation 4

What would IPTG doif it is added to aculture of bacteriagrowing on poorlyutilized carbonsource?

It would bringconformationalchange in thestructure of inducermolecule.

It would induce thelac operon enzymes.

It would bringconformational change inthe structure of repressormolecule.

It would repress the lacoperon enzymes.

3

GACNNN/NNGTC isthe restriction site forAspEI and AhdI.

Therefore, the twoenzymes can beknown as:

Neoschizomers Isoschizomers Isocaudomer Blunt-end isomers 2

Aat II cuts at(GACGT/C) and Zra Icuts at (GAC/GTC).

Therefore, they canbe known as:

Isocaudomers Neoschizomers Isoschizomers Blunt-end isomers 2

Which of thefollowing is used asa tag for bases inautomated DNAsequencing?

Radioactive tag Fluorescent tag Antibodies Tracer dyes 2

What areneoschizomers?

Same restrictionenzymes thatrecognize differentsequences.

Different restrictionenzymes thatrecognize differentsequences.

Different restrictionenzymes that recognize thesame sequence.

Same restrictionenzymes that recognizesame sequences.

3


14 of 15


15/15



At which of the

following positionsdoes doublestranded LambdaDNA has (a) smallsingle strandedregion(s)?

3' end 5' end It does not have a single

stranded region.

In the middle of the

DNA sequence.

2

Which of thefollowing is true forpreproteins?

They needposttranslationalmodifications tobecome active.

They are proteinswithout signalpeptide.

They need posttranscriptionalmodifications.

They need bothpost-translational andpost-transcriptionalmodifications.

1

Which of thefollowing is NOT agene family?

Homeobox Achaete-scutecomplex

Major histocompatibilitycomplex (MHC)

All of the above belongto gene family.

4

Which of thefollowing is true for

Rifampicin?

It inhibits DNApolymerase.

It preventstranscription of DNA

to RNA.

It is lipophobic in nature. It allows transcriptionbut inhibits at the

translation.

2

Which among thefollowing screeningprocedure is highlysuited to findadjacent genomic

clones from alibrary?

Hybrid arrest Colony and plaquehybridization

Chromosome walking Primer walking 3

Transgenicorganisms are:

The ones thatcontain a foreign

gene and extracopies ofendogenous gene.

Geneticallymanipulated

organisms thatcontain multipleforeign genes.

Genetically manipulatedorganisms that contain

multiple foreign genes,extra copies ofendogenous gene.

The ones that contain acopy of endogenous

gene and multiplecopies of foreign genes.

3

Which of thefollowing is true fora retrotransposon?

It has a structuresimilar to retroviralgenome.

It encodes proteinssimilar to retroviralreverse transcriptase.

It is flanked by longterminal repeatsequences(LTR).

It is a genetic elementwhich can amplify itselfon a genome.

4

Which of thefollowing statementsis false regardingsatellite DNA?

Prokaryotes aredevoid of it.

It forms a variableproportion of thetotal DNA,depending on thespecies.

It is composed of shortrepetitive sequences.

It is found in theeuchromatic regions ofchromosomes.

4

Which of thefollowing statementsis true about pairedend sequencing?(i) Paired endsequencing is alsocalled double barrelshotgun sequencing.(ii) Paired endsequencing is usedto overcome thedifficulty of havingrepetitive DNAsequences.(iii) Paired endsequencing permitsthe assembly of largegenome scaffolds.

(i) and (ii) (ii) and (iii) (iii) only (i) only (i), (ii) and (iii) 5

_____________ isthe process ofsecondary antibodylinked to an enzyme.

Biotransformation Bioconjugation Biotransduction Biotransfusion 2

The proteinbeta-galactosidase isencoded by a genecalled

____________.

lacI lacY lacZ lacA 3


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Interview Questions - Set 2

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