Intraosseous Injection of rhBMP-2/Calcium Phosphate Matrix Improves Bone Structure and Strength in the

Proximal Aspect of the Femur in Chronic Ovariectomized Nonhuman Primates

by Howard J. Seeherman, X. Jian Li, Erica Smith, Jascha Parkington, Rebecca Li, and John M. Wozney

J Bone Joint Surg AmVolume 95(1):36-47

January 2, 2013

©2013 by The Journal of Bone and Joint Surgery, Inc.

Radiographic appearance of a femur treated with 1.5 mg/mL rhBMP-2/CPM (Fig. 1-A) and a femur treated with CPM alone (Fig. 1-B) prior to surgery, immediately after surgery, and at two and six

months after surgery.

Howard J. Seeherman et al. J Bone Joint Surg Am 2013;95:36-47

©2013 by The Journal of Bone and Joint Surgery, Inc.

Anteroposterior μCT appearance of a femur treated with 1.5 mg/mL rhBMP-2/CPM (Fig. 2-A) compared with that of the contralateral, untreated femur (Fig. 2-B); and the appearance of a

femur treated with CPM alone (Fig. 2-C) compared with that of the contralate...

Howard J. Seeherman et al. J Bone Joint Surg Am 2013;95:36-47

©2013 by The Journal of Bone and Joint Surgery, Inc.

Histological appearance of the femoral neck region at one month (Figs. 3-A and 3-B), three months (Figs. 3-C and 3-D) and six months (Figs. 3-E and 3-F) in a femur that was treated with

rhBMP-2/CPM as compared with the appearance of the femoral neck region ...

Howard J. Seeherman et al. J Bone Joint Surg Am 2013;95:36-47

©2013 by The Journal of Bone and Joint Surgery, Inc.

Histological appearance of the proximal part of the femur three months after treatment with 1.5 mg/mL rhBMP-2/CPM (closed arrows).

Howard J. Seeherman et al. J Bone Joint Surg Am 2013;95:36-47

©2013 by The Journal of Bone and Joint Surgery, Inc.

Histological appearance of the femoral head-neck junction of a 1.5 mg/mL rhBMP-2/CPM-treated femur at six months (Figs. 5-A and 5-B).

Howard J. Seeherman et al. J Bone Joint Surg Am 2013;95:36-47

©2013 by The Journal of Bone and Joint Surgery, Inc.

Histological appearance of the contralateral untreated femoral head-neck junction of a femur at six months (Figs. 6-A and 6-B).

Howard J. Seeherman et al. J Bone Joint Surg Am 2013;95:36-47

©2013 by The Journal of Bone and Joint Surgery, Inc.