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Page 1 Introducing the Agilent 7000A QQQ-MS for GC Sunil Kulkarni Product Specialist Agilent Technologies
Page 1
Introducing the Agilent 7000A QQQ-MS for GC
Sunil KulkarniProduct SpecialistAgilent Technologies
Page 2
Agilent GC/MS Portfolio
Smallest footprint, single GC column
Industry leading GC/MS System
5975C with 7890GC
5975VL with 6850GC
Page 3
Agilent GC/MS Portfolio
Smallest footprint, single GC column
Industry leading GC/MS System
5975C with 7890GC
5975VL with 6850GC
Introducing
Page 4
Agilent GC/MS Portfolio
Power of MRM7000A GC/MS/MS
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Agilent 7000A GC/MS/MS
7000A Width36 cm/14 inches
Only 2 inches wider than the 5975C MSD
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GC/MS Triple Quad (QQQ)
DetectorQuad 1Mass Analysis
Collision Gas (Ar, N2, He)
Quad 2Collision Cell
Ion Source•Ionize Quad 3
Mass Analysis
Carrier Gas (He, H2 )
Elements of a typical triple quadrupole MS system
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Agilent 7000 GC/MS/MS
DetectorQuad 1
Collision Gas (N2 )
Hexapole Collision Cell
Ion Source•Ionize Quad 2
The hexapole field has excellent transmission effic iency for precursor and product ions
Q1 Post-filter
Q2 Pre-filter
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What is MRM MS/MS?Multiple Reaction Monitoring
EI: many ions from the source
Q1 SIM isolate precursor ion
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What is MRM MS/MS?Multiple Reaction Monitoring
Product 3
Product 2
EI: many ions from the source
Product 1
EI-MS/MS
Q1 SIM isolate precursor ion
CID + Q2 SIM
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Why MS/MS?Lower detection limits by reducing noise
EI: many ions from the source
Q1 SIM isolate precursor before CID
chemical noise eliminated
Product ion signal often decreases, but the percentage decrease in noise is much larger for real samples; S/N and detection limits improve
Product 1Product 3
Product 2
CID +Q2 SIM
Product ion measured against zero chemical noise
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Why MS/MS?Greater Selectivity Than SIM
EI-SIMselectivity proportional to
spectral resolutionno selectivity against ions
with same m/z
interference
analyte
unit mass resolution
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Why MS/MS?Greater Selectivity Than SIM
PrecursorIon
Product 1Product 3
Product 2
EI-SIMselectivity proportional to
spectral resolutionno selectivity against ions
with same m/z
EI-MS/MSPrecursor selectivity same as SIM
High probability that at least one product ion will be a unique dissociation product
of the precursor BUT not the interferenceinterference
analyte
interference
The precursor ion should NOT be used for ion ratios or quantitation since the interferences will
be the same as the SIM ion
unit mass resolution
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Why a GC/MS/MS System?
Allows for the selective quantitation of target compounds in high chemical background samples
Better S/N in complex matrices than can be achieved by single quadrupole scan or SIM approaches.
Newer regulations in some markets specify analytical power commensurate with GC/MS/MS
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Why GC/MS/MS? A Picture Is Worth a Thousand Words
GC/MS/MS QQQ MRM
GC/MS Single Quad SIM
EI 100fg HCB in “DIRTY” Matrix
S/N: 116:1 RMS
100 fg HCB
MS SIM
MS/MS MRM A chromatographer’s dream: single peak on flat baseline
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Why GC/MS/MS? A Picture Is Worth a Thousand Words
GC/MS/MS QQQ MRM
GC/MS Single Quad SIM
EI 100fg HCB in “DIRTY” Matrix
S/N: 116:1 RMS
100 fg HCB
MS SIM
MS/MS MRM A chromatographer’s dream: single peak on flat baseline
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Technology
Agilent took the best technologies from their industry leading 597X Series GC/MSD:
– Heated monolithic gold plated quartz quadrupole
– Proven reliable high performance source design
– AUTOTUNE
and the 6410A LC/QQQ:
– Linear acceleration enhanced Collision Cell
– Wide Mass-Bandwidth QQQ ion optics
– Mass Hunter software
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Why Heated Quartz “Gold” Quads?
Unlike LC/MS, many high boiling neutral molecules enter the source and manifold of a GC/MS or GC/MS/MS
• Higher temperature reduces potential for contamination– 200C max
– Virtually eliminates the need to clean quads
• Low coefficient for thermal expansion for quartz– Stable structure during maintenance cycles (hot-cool-hot) for source
or detector
More stable tunes and methods over a longer period of time in real world sample environments
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GC/QQQ Analyzer
Inert, high temp source (same as 5975)
Triple-Axis detector (same as 5975)
Two gold plated quartz, hyperbolic analyzers (same as 5975)
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GC/QQQ Analyzer
Shown with shields for analyzers and collision cell
New high performance, hexapole collision cell with patent technology
EdwardsSplit Flow Turbo
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Analyzer Doors Open
Triple-Axis Detector
Inert, 350ºC Source
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Proven Inert Source Performance
Several thousands sources in current use
Stay-Clean design extends maintenance intervals with dirty samples
Source tune parameters in the Autotune file
Dual filament design reduces maintenance intervals
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EI Source Details
Partition between source and analyzer compartments
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High Performance Collision Cell Design
Linear acceleration design is optimized for high speed performance without ion ghosting or cross-talk
MRM speed to 500 MRMs/sec allows determination of more compounds per ion group
High sensitivity with wide mass bandwidth eliminates the need to “tune on your compound” for optimum sensitivity
“Helium Quenching” chemical noise reduction increases S/N
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Collision Cell Details
Quadrupole post-filter
Quadrupole pre-filter
Support cradle
(Cell housing and 2 rods removed for clarity)
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High Performance Triple-Axis Detector
Ultra low neutrals noise
Long life and high linearity
“Gain Normalization” corrects tune file for detector aging to allow repeatable long term method sensitivity
Same design as used in the 5975C GC/MSD
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Triple-Axis Detector
hyperbolic quartztransmission
quadrupoleanalyzer
triple channelelectron multiplier
high energy dynode
steering rod
ion beam
X
Y
OffsetZ
shield for secondary particles
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Autotune
Proprietary program tunes the source, mass analyzer, and detector for (as applicable):
• Ion transmission
• Mass axis calibration
• Mass resolution
• Detector gain vs Voltage
Autotune settings are saved with the method for repeatable method performance.
Manual Tune override is available
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Agilent 7000A GC/MS/MS OFN (octafluoronaphthalene) Sensitivity
272:241 Transition186:1 RMS S/N
100fg OFN
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One of the many innovations that led to this level of GC/MS/MS
sensitivity
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Collision Cell Gas Flows
1 ml/min N2 Collision Gas
Q1 Ions In
Collision Cell Ions OutHe* +
He* + N2 → N2+. + He + e-
N2+. + A → A+. + N2
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Collision Cell Gas Flows
1 ml/min N2 Collision Gas
Q1 Ions In
Collision Cell Ions OutHe* +
He* + He → 2 He + heat
He* + N2 → N2+. + He + e-
He Buffer Gas
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Effect of “Helium Quench”
S/N 341:1 Helium Quench Gas OFF
tested with hexachlorobenzene
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Effect of “Helium Quench”
S/N 1294:1 Helium Quench Gas ON
S/N 341:1 Helium Quench Gas OFF
Almost 4 XIncrease in S/N
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Effect of “Helium Quench”
Noise Comparison
S/N 1294:1 Helium Quench Gas ON
S/N 341:1 Helium Quench Gas OFF
tested with hexachlorobenzene
The increase in S/N was due to reduction of noise.
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MassHunter MS Workstation Software
Modern software interpretation of the proven industry standard GC/MS Chemstation platform
Single software platform for all Agilent MS Systems
• LC/SQ , LC/QQQ, LC/TOF, LC/QTOF
• GC/SQ, GC/QQQ
• ICP/MS
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GC/MS/MS Software Modules
Acquisition
Analysis
Excel Reporting
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MS/MS Acquisition Method Editor Dialog
Single window acquisition setup
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Agilent G7000A GC/QQQ
Outstanding sensitivity 100fg of OFN on column at 100:1 S/N RMS in MS/MS mode using AUTOTUNE parameters verified at customer installation.
1050 amu Mass Range
500 MRM/sec Speed
Reliable heated monolithic gold plated hyperbolic quadrupoles
Differentially pumped vacuum system
New Helium seeded collision cell technology
Agilent 7890 GC with Capillary Flow technology.
MassHunter Software
