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Copyright © Proxeon Biosystems A/S2
Overview
The EASY-nLC™ is:
• Complete• Configured• Compact• EASY to
– install– learn– operate
• Performance
Copyright © Proxeon Biosystems A/S3
Complete
• 1 cooled autosampler– 96 or 384 well plates– Single or double height– Wash & Waste bottles
• switching valves
• nano-flow sensor
• nano-litre pumps
• Built-in PC & software
Copyright © Proxeon Biosystems A/S4
EASY2-nLC™ (with flow control)2-column
Waste
Solvent A
Solvent B
AFC™
AdvancedFlowControl system
Copyright © Proxeon Biosystems A/S5
EASY2-nLC™ (with flow control)1-column
Solvent A
Solvent B
AFC™
AdvancedFlowControl system
Copyright © Proxeon Biosystems A/S7
Compact
• Small footprint – 35cm wide (14”)– 38cm deep (15”)– 45cm high (18”)
• Touch-screen controlled– built-in computer– no keyboard or mouse
required
• Light– 35 kg, all inclusive
Copyright © Proxeon Biosystems A/S8
Easy to install• Only a few cables required
– contact closure– [network is optional]– Power
• Software is – integrated, – pre-installed and – up-to-date
• Solvent purging, air removal and column flushing is fully automated
• Less than 4 hours from power up to first analytical data
Copyright © Proxeon Biosystems A/S9
Easy to learn
• Logical software structure• Practice oriented work flow
• Results oriented parameters– E.g. volume & flow rates,
not hardware control• Intuitive graphical
user interface
Copyright © Proxeon Biosystems A/S10
Easy to operate (1)
• What You See Is What You Get– Simple design– Logical layout– Clear separation
in compartments– Modular
components
• Easy access to all parts of instrument
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Easy to operate (2)
• Automated maintenance scripts
• Intelligent trouble shooting software
• Web interface for software upgrades
Copyright © Proxeon Biosystems A/S12
Easy to operate (3)
• Split-less, nano-flow means:• Less solvent use• Less waste
• Robust design & Intelligent software control means:
• Less support • Less maintenance • More uptime
M T W T F S S
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M T W T F S S
1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
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28 29 30
Copyright © Proxeon Biosystems A/S13
Example (1)• Mass spectrometer
– Finnigan LCQ Classic• Pre-column
– 2cm, 75µm ID, Reprosil-Pur C18-AQ. 5µm
• Analytical column– 10cm, 75µm ID, Reprosil-Pur
C18-AQ. 3µm• Solvents
– A: 0.005% HFBA, 0.4% acetic acid, water
– B: 0.005% HFBA, 0.4% acetic acid, 90% acetonitril, water
• Amount injected– 100 fmol tryptic digested BSA
• HPLC gradient– 300 nl/min, 10-100 %B in 25
minutes
6 out of 36 consecutive runs
Copyright © Proxeon Biosystems A/S14
Example (2)
• Carry-over– < 0.003%
• Sample pickup– From 0.2µl to full loop in
0.01µl steps– Loop size adjustable– RSD < 0.5%
• Peak widths (FWHM)– < 10-15s at 50%
• Reproducibility– RT RSD < 0.7%