RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures
Introducing the SCIEX Triple Quad™ and QTRAP® 6500+ LC-MS/MS System with optional SelexION®+ Technology
SCIEX, Concord, Ontario (Canada)
2 © 2015 AB Sciex RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures
Our Best Triple Quad Just Got Better – the Most Sensitive and Fastest SCIEX Triple Quad™ System
The New SCIEX 6500+ Series with IonDrive™ Technology
Improved IonDrive™ detection system
• Detect analytes across a wide scope of
chemistries, low mass or high mass, positive or
negative polarity
• Quantify with lowest limits of quantitation and up
to 6 orders of linear dynamic range
• Ultra fast polarity switching with Scheduled
MRM™ Pro algorithm for multi-target
quantitation
Elevated SelexION® technology
• Jet Injection technology with improved
transmission and no effect on resolution
Audible noise reduction
Industry proven performance of IonDrive™
Turbo V ion source and QJet® ion guide
Results in less time – fast, high-throughput,
simplified data processing tools
Service and support resources for success
New
New
New
RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures
Hardware Overview
4 © 2015 AB Sciex RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures
Improved IonDrive™ Detection System
• New detector ‒ Larger detection area
• New power supply ‒ High energy dynode at 15 kV
improves sensitivity
‒ Enables faster 5 msec polarity switching
• “Floated” design ‒ Ions in negative polarity directed straight into the detector
‒ This benefits low mass ions which are detected much more efficiently than
in the 6500 system
Contributes to significant signal gains in negative mode Q3 fragment
ion <100 Da
S/N (LOQ) improvements are compound, matrix and LC dependent
Improved data quality in screening applications with ultra-fast 5 msec
polarity switching
5 © 2015 AB Sciex RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures
Sensitivity of 6500+ System in Positive Polarity
0.1 pg/mL Testosterone (with SelexION®+ Technology, 50 µL Injection)
6 © 2015 AB Sciex RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures
Linearity of 6500+ System in Positive Polarity
0.1 to 100 pg/mL Testosterone (with SelexION®+ Technology)
0.1 to 5 pg/mL
Accuracies: 92 to 109%
%CV: 3.8% at 0.1 pg/mL
7 © 2015 AB Sciex RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures
Linearity of 6500+ System in Positive Polarity
1 to 100,000 pg/mL Flurazepam (with SelexION®+ Technology)
1 to 1000 pg/mL
5 orders
r > 0.9999
8 © 2015 AB Sciex RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures
Sensitivity of 6500+ System in Negative Polarity
1 ng/L (1 part-per-trillion) PFOA and PFOS (Linearity 0.5 to 5000 ng/L)
Quantifier (blue)
Qualifier 1 (purple)
Qualifier 2 (orange)
Quantifier (blue)
Qualifier 1 (purple)
PFOA
PFOS
499/80 499/99
413/369 413/219 413/169
9 © 2015 AB Sciex RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures
100 ppt PFBS
QTRAP® 5500 LC-MS/MS system
Sensitivity of 6500+ System in Negative Polarity
QTRAP® 6500+ LC-MS/MS system
MRM 298.9 / 80.0
~ 2x S/N improvement
MRM 298.9 / 99.0
~ 2.5x S/N improvement
10 © 2015 AB Sciex RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures
Sensitivity of 6500+ System in Negative Polarity
0.1 ng/mL (100 ppt) Monofluoroacetate (Linearity 0.05 – 1000 ng/mL)
77/57 (blue)
77/33 (purple)
Internal standard
81/60 (blue)
81/35 (purple)
Accuracies: 96.1 to 110.9%
%CV: 4.99% at 0.1 pg/mL (n = 10)
11 © 2015 AB Sciex RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures
Ultra Fast Polarity Switching of 6500+ System
Multi-Pesticide Screening for ~300 Analytes with 5 msec Switching
552 MRM transitions
Positive ESI
58 MRM transitions
Negative ESI
Flufenacet CV = 4.37%
Methoxyfenozide CV = 1.52%
12 © 2015 AB Sciex RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures
Multi-Pesticide Screening with 5 msec Polarity Switching
Pesticide Standard and Sample Extracts (Positive Polarity)
1 ng/mL
Tomato (10x)
Banana (10x)
Lemon (10x)
Cucumber (10x)
Strawberry (10x)
Arugula (20x)
13 © 2015 AB Sciex RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures
Multi-Pesticide Screening with 5 msec Polarity Switching
Pesticide Standard and Sample Extracts (Negative Polarity)
1 ng/mL
Tomato (10x)
Banana (10x)
Lemon (10x)
Cucumber (10x)
Strawberry (10x)
Arugula (10x)
RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures
Software Overview
15 © 2015 AB Sciex RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures
Quantitation and Ion Ratio in MultiQuant™ Software
QuEChERS Extract of Cucumber (dSPE with PSA) and 10 x Dilution
Std. 1 Lemon Tomato Cucumber Strawberry Arugula
Propamocarb
16 © 2015 AB Sciex RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures
QTRAP® Library Searching in MasterView™ Software
QuEChERS Extract of Cucumber (dSPE with PSA) and 10 x Dilution
Sample
MS/MS FIT = 100%
Standard
17 © 2015 AB Sciex RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures
Quantitation and Ion Ratio in MultiQuant™ Software
QuEChERS Extract of Strawberry (dSPE with PSA) and 10 x Dilution
Std. 1 Lemon Tomato Cucumber Strawberry Arugula
Dimethoate
18 © 2015 AB Sciex RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures
QTRAP® Library Searching in MasterView™ Software
QuEChERS Extract of Strawberry (dSPE with PSA) and 10 x Dilution
Sample
MS/MS FIT = 100%
Standard
19 © 2015 AB Sciex RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures
Quantitation and Ion Ratio in MultiQuant™ Software
QuEChERS Extract of Strawberry (dSPE with PSA) and 10 x Dilution
Std. 1 Lemon Tomato Cucumber Strawberry Arugula
Fludioxonil (-)
RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures
SelexION®+ Technology Differential Mobility Spectrometry (DMS)
21 © 2015 AB Sciex RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures
DMS Electrode 1
DMS Electrode 2
DMS Electrode 1
DMS Electrode 2
Lens
Lens
SelexION®+ Ion Mobility Device with Jet Injector Technology
• New DMS cell designed to improve ion
transmission by ~2x without loss in
selectivity or resolution
• Addition of lens increases ion velocities
into cell
• Reduces transit times through
detrimental fringing fields
• Only available on 6500+ platform
Continually driving selectivity improvements
SelexION cell
SelexION+ cell
DMS Electrode 1
DMS Electrode 2
DMS Electrode 1
DMS Electrode 2
Lens
Lens
22 © 2015 AB Sciex RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures
6500+ DMS removed
Detection of Triazole with and without DMS
6500+ with DMS
Standard
0.5 ng/L
Spiked and extracted fruit
(10 µg/kg)
S/N ~ 390
S/N ~ 60
IS = 3000 V; TEM = 550ºC
N
NHN
23 © 2015 AB Sciex RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures
Accuracy 97-103%
%CV < 2%
Linearity and Repeatability (0.01 mg/kg – 50 mg/kg; n = 3)
24 © 2015 AB Sciex RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures
Added Selectivity to Simplify Sample Extraction
Example: 1 µg/kg Imidacloprid and Metabolites in Bee Pollen Analyzed
using QTRAP® 6500+ System with SelexION®+ Technology
Pollen extract 4x dilution, average sensitivity gain over 6500 with DMS = 3x
Imidacloprid Imidacloprid-IS
5-OH
Olefin
25 © 2015 AB Sciex RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures
Detection of Somatostatin with and without SelexION+
6500+ DMS removed 6500+ with DMS
Somatostatin, a cyclic peptide with poor MS/MS fragmentation is acquired using MIM (819.7/
819.7) assay in rat plasma showing high background noise limiting the S/N ratio and LOQ
Highly reduced background noise enabling desirable LOQ of 100 pg/mL using new SelexION+ jet
injector on 6500+ System (S/N ratio = 12, accuracy = 101% and %CV < 10% )
100 pg/mL
26 © 2015 AB Sciex RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures
Industry Proven Robustness and Ruggedness
Alprazolam %CV = 3.5%
Buprenorphine %CV = 7.9%
Clenbuterol %CV = 2.9%
Methamphetamine %CV = 2.8%
Repeatability of 4000 injections (crashed human plasma (1:3) diluted 1:2)
SelexION®+, IonDrive™ Turbo V Ion Source and QJet® Ion Guide
27 © 2015 AB Sciex RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures
Industry Proven Robustness and Ruggedness
Alprazolam
Buprenorphine
Clenbuterol
Methamphetamine
Repeatability of 4000 injections (crashed human plasma (1:3) diluted 1:2)
1. Injection 1000. Injection 2000. Injection 3000. Injection 4000. Injection
28 © 2015 AB Sciex RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures
Audible Noise Reduction
• Active (source exhaust modifications) ‒ Source exhaust flow minimized to match gas load
‒ Current shipping design assumes maximum
incoming source gas load at all times
• Passive (quieter tubing) ‒ Eliminate “whistling” noise
• Quieter system operation than 6500 ‒ Noticeable under most source conditions
‒ No impact to sensitivity
Decreased audible noise for a more comfortable work
environment
29 © 2015 AB Sciex RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures
The New SCIEX 6500+ Series and SelexION+ technology
Industry proven performance: IonDrive™ Turbo V ion source
and QJet® ion guide
• Significant sensitivity boost in low mass
negative mode with “new HED+ detector
technology”
• Enhanced SelexION+ performance with
“new jet injector DMS cell” without
compromising resolution
• Fast polarity switching speed (5 msec)
• Audible noise reduction
30 © 2015 AB Sciex RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures
Trademarks/Licensing
For Research Use Only. Not for use in diagnostic procedures.
© 2015 AB Sciex. The trademarks mentioned herein are
the property of AB Sciex Pte. Ltd. or their respective owners.
AB SCIEX™ is being used under license.