Date post: | 23-Dec-2015 |
Category: |
Documents |
Upload: | sophie-wade |
View: | 224 times |
Download: | 1 times |
Parts of the microscope Eyepiece (9)- the
lens you look through, magnifies your specimen – 10x
Body tube (1)-supports the eyepiece
Arm (10)-supports body tube
Nose piece (2)- holds objective lenses
Objectives (3, 4, 5)- magnify by different powers
Low power objective=4X Medium power
objective=10X High power objective=40X
Stage Clips (6)- holds the slide in place
Aperature-allows light to show through(draw it on the diagram)
Stage (11)- supports the slide being used
Diaphragm (7)- controls the amount of light passing through the opening of the stage (light adjustment wheel)
Course Adjustment knob (12)- moves body tube up and down for focusing, low power
Light source (8)
Fine Adjustment knob (13)- moves body tube up and down for finer focusing, high power
COMPOUND LIGHT MIRCROSCOPE
Microscopes use lenses to make small objects look larger.
Microscopes must combine magnification and resolution. Magnification - the ability to
make objects look larger than they are
Resolution -the ability to clearly distinguish the individual parts of an object
Safety and Proper Use ALWAYS carry microscope
with two hands – one on the arm and one under the base
Report any problems with microscope first thing
Never touch lenses with finger/hand
Remove slides before returning microscope
NEVER use the Coarse adjustment knob on high power
Total MagnificationTotal Magnification
What is What is total magnification?total magnification? The sum power of all the lenses you are
viewing the specimen with. How do you calculate total
magnification? Eyepiece x objective lens = total mag.
Ex. Our microscopes using medium power. 10 x 10 = 100x (objects appear 100 times
larger than they are.)
Using the Microscope When using the scopes to view slides
it is important to remember to keep the specimen centered!
Keep in mind your field of view Field of View-the area visible through
the microscope lens. Field of view DECREASES as magnification INCREASES.
Adjust the diaphragm as you increase magnification, field of view gets darker.
Using the Microscope Because the light rays from an
object cross before reaching your eye, the image you see through our light microscopes will be inverted and upside down as well as larger and more detailed..
Letter “b” as seen on the stageLetter b as seen through the eyepiece
Image inversion
Because of this image inversion it is important to remember
-When you move your slide right it will move left in the field of vision
-When moving the slide up it will move down in the field of vision