iìãÉå OMM pÉêáÉë Advanced Fluorescence Illumination Part Number Shutter/ Attenuator Filter Wheel Spectral Range L200## Manual No Standard Microscopy L210## Manual No Tuned for Cy5 and Cy5.5 L220## Manual No Extended in UV, Fura II and IR,Cy7 L200P## Motorised Motorised 6 position Standard Microscopy L210P## Motorised Motorised 6 position Tuned for Cy5 and Cy5.5 L220P## Motorised Motorised 6 position Extended in UV, Fura II and IR, Cy7 Options available: Lumen 200 is the latest product for fluorescence illumination to be designed and manufactured by Prior Scientific. Prior have been making illumination systems for fluorescence for over 20 years. The experience gained over this period has enabled Prior to design the most powerful, cost effective and flexi- ble system on the market. Fluorescence has never been so easy. Powerful 200W Illumination Long Life Lamp - Up to 2000 Hours Easy Lamp Replacement Flexible Liquid Light Guide Low Heat Transfer Shutter and Filter Wheel Options Part numbers are up to 8 characters long, the microscope specific digits have been removed and replaced with #. Consult the Prior Configurator or contact your local representative for further details on part numbers for specific microscopes.
L220## Manual No Extended in UV, Fura II and IR,Cy7
L200P## Motorised Motorised 6 position Standard Microscopy
L210P## Motorised Motorised 6 position Tuned for Cy5 and Cy5.5
L220P## Motorised Motorised 6 position Extended in UV, Fura II and IR, Cy7
Options available:
Lumen 200 is the latest product for fluorescence illumination to be designed and manufactured by Prior Scientific. Prior have been making illumination systems for fluorescence for over 20 years. The experience gained over this period has enabled Prior to design the most powerful, cost effective and flexi-ble system on the market. Fluorescence has never been so easy.
Powerful 200W Illumination Long Life Lamp - Up to 2000 Hours Easy Lamp Replacement Flexible Liquid Light Guide Low Heat Transfer Shutter and Filter Wheel Options
Part numbers are up to 8 characters long, the microscope specific digits have been removed and replaced with #. Consult the Prior Configurator or contact your local representative for further details on part numbers for specific microscopes.
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Powerful 200W Illumination A 200W metal-halide lamp gives a simi-lar spectral output to older mercury vapour lamps and the metal-halide has over seven times longer lamp life.
Long Life Lamp - Up to 2000 Hours The metal-halide lamp is safe for use for up to 2000 hours depending on opera-tional factors. Internal electronics monitor the number of hours used and warn the user through both a visual and audible alarm, when a lamp replacement is necessary.
Easy Lamp Replacement Unlike mercury vapour lamps the lamps used in the Lumen 200 system require no precision arc alignment for them to work properly. Lamps are pre-centred, so changes take less than one minute.
Flexible Liquid Light Guide A highly efficient liquid light guide delivers full spectrum illumination from 350nm upwards. Couplers are avail-able for all research micro-scopes.
Low Heat Transfer By mounting the light source remotely from the micro-scope stand the amount of heat transferred is signifi-cantly lower. This aids sta-bility and helps reduce drift due to thermal expansion in long term time lapse experi-ments.
Manual Shutter & Attenuation The Lumen 200 features a manually operated shutter mechanism that can be partially closed to attenuate illumination intensity protecting valuable samples from photo-bleaching.
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Stable Light Output Over 2000 Hour Lifetime The Lumen 200 is engineered specifically to keep the 200W lamp at optimum temperature. This delivers unrivalled stability both in long term and short term output.
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Lamp Lifetime The specially designed lamp has a linear degradation over 2000 hours of use, this provides a stable light source for quantitative and qualita-tive analysis.
The three available options for the Lumen are: L200 Standard microscopy L210 Tuned for Cy5 and Cy5.5 L220 Extended in UV and IR for Cy7
L210 Tuned for Cy5 and Cy5.5. The highlighted section of the above graph shows the improved emission be-tween 600nm and 700nm.
L220 Extended in UV for Fura II and IR for Cy7. The highlighted section of the above graph shows the improved emission between 300nm and 400nm. Applications include FISH and intercellular Cal-cium investigations with Fura II ratiometric inves-tigations.
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PN: DS08013 Ver 1 Issue 01/11/08
VISIT PRIOR ON THE WEB AT WWW.PRIOR.COM Specifications are subject to change without notice.
Prior Scientific Limited Cambridge, UK. T. +44 (0) 1223 881711 E. [email protected]
Prior Scientific Inc. Rockland, MA, USA. T. +1 781-878-8442 E. [email protected]
Prior Scientific GmbH Jena, Germany. T +49 (0)3641 675 650 E. [email protected]
Shutters & Attenuation The simple, robust and efficient design of the shuttering light attenuator provides both high speed and reliability. The less than 20 ms open-ing and closing speed will help to extend live cell studies by minimising the toxicity associated with photo-bleaching. With only one moving part the stepper motor driven shutter is de-signed to provide years of reliable un-interrupted service.
High Speed Motorised Filter Wheel The Lumen 200PRO features a built in, six posi-tion filter wheel compatible with all standard 25mm diameter filters for automated control of excitation wavelength. The unit is driven by the Prior range of ProScanTMII, OptiScanTMII and Prior PCI II advanced motor controllers allowing it to be combined with the highest performance scanning stages and focusing devices available. High speed filter changing with adjacent positions changed in 60ms with the ProScanTMII controller.
Software Compatibility Controlled by either the Prior ProScanTMII, OptiScanTMII or PriorPCI II makes adding the Lumen 200PRO to existing code controlling Prior Scientific automation equipment quick and easy. The Lumen 200PRO’s internal filter wheel responds to existing standard filter wheel com-mands whether through the DLL or via ASCII commands, and light intensity is controlled with one additional command.
Advanced Fluorescence Illumination with Integral Automated Light Attenuation and High Speed Filter Wheel The Lumen 200Pro features a built in, six position filter wheel for automated control of excitation wavelength. Lumen 200Pro also includes a variable aperture motorised shutter allowing computer controlled shuttering and light attenuation. The unit is driven by the Prior range of ProScanTMII, OptiScanTMII or PriorPCI II advanced motor controllers, allowing it to be combined with the highest perform-ance scanning stages and focusing devices available. By removing the filter wheel and shutter from direct contact with the microscope stand problems of vibration, heat transfer and long settling times are practically eliminated.
One of the common problems with the conventional fluorescence microscope light source is optimising and maintaining its proper adjustment to provide even illumination over the whole field of view. Uneven illumination becomes much more of a problem when using digital imaging. This is because contrast stretching used to bring out the signals from the background also enhances uneven illumination across the image. Note how the chromosomes in the images taken using a conventional lamp become darker around the periphery of the image, the red signal on the left is nearly lost altogether.
