Disclosure to Promote the Right To Information Whereas the Parliament of India has set out to provide a practical regime of right to information for citizens to secure access to information under the control of public authorities, in order to promote transparency and accountability in the working of every public authority, and whereas the attached publication of the Bureau of Indian Standards is of particular interest to the public, particularly disadvantaged communities and those engaged in the pursuit of education and knowledge, the attached public safety standard is made available to promote the timely dissemination of this information in an accurate manner to the public. इंटरनेट मानक “!ान $ एक न’ भारत का +नम-ण” Satyanarayan Gangaram Pitroda “Invent a New India Using Knowledge” “प0रा1 को छोड न’ 5 तरफ” Jawaharlal Nehru “Step Out From the Old to the New” “जान1 का अ+धकार, जी1 का अ+धकार” Mazdoor Kisan Shakti Sangathan “The Right to Information, The Right to Live” “!ान एक ऐसा खजाना > जो कभी च0राया नहB जा सकता ह ै” Bhartṛhari—Nītiśatakam “Knowledge is such a treasure which cannot be stolen” IS 2558 (1994): Ponceau 4R, Food Grade [FAD 8: Food Additives]
Transcript
Disclosure to Promote the Right To Information
Whereas the Parliament of India has set out to provide a practical regime of right to information for citizens to secure access to information under the control of public authorities, in order to promote transparency and accountability in the working of every public authority, and whereas the attached publication of the Bureau of Indian Standards is of particular interest to the public, particularly disadvantaged communities and those engaged in the pursuit of education and knowledge, the attached public safety standard is made available to promote the timely dissemination of this information in an accurate manner to the public.
इंटरनेट मानक
“!ान $ एक न' भारत का +नम-ण”Satyanarayan Gangaram Pitroda
“Invent a New India Using Knowledge”
“प0रा1 को छोड न' 5 तरफ”Jawaharlal Nehru
“Step Out From the Old to the New”
“जान1 का अ+धकार, जी1 का अ+धकार”Mazdoor Kisan Shakti Sangathan
“The Right to Information, The Right to Live”
“!ान एक ऐसा खजाना > जो कभी च0राया नहB जा सकता है”Bhartṛhari—Nītiśatakam
“Knowledge is such a treasure which cannot be stolen”
“Invent a New India Using Knowledge”
है”ह”ह
IS 2558 (1994): Ponceau 4R, Food Grade [FAD 8: FoodAdditives]
IS 2558 : 1994
~ JflrtCfJ
-qrm 4 3m,~ "!ts - ~( ?iil'!f TRterar )
Indian StandardPONCEAlJ 4R, FOOD GRADE - SPECIFICATION
( Second Revision)
Second Reprint NOVEMBER 1997
UDC 644.099.62 : 668.811.1 PON
co BIS 1994
BUREAU OF IN'DIAN STANDARDSMANAK BHAVAN, 9 BAHADUR SHAH ZAFARMARG
NEW DELHI 110002
October 1994 Price Groul) 2
Reaffirmed 2009
AMENDMENT NO.1 MAY 2008TO'
IS 2558 : 1994 PONCEAU 4R, FOOD GRADE SPECIFICAnON
[Page 1, Table 1,Sl No. (iv), col 3] - Substitute '0.2' for '0.4'.
(FAD 8)
RCiH'Oii"IPhY unit, aiS, NewDelhlliidia
Food Additives Sectional Committee. FAD 8
FOREWORD
This Indian Standard ( Second Revision) was adopted by the Bureau of Indian Standards. after thedraft finalized by the Food, Additives Sectional Committee had been .approved by the Food andAgriculture Division Council. .
This standard is one of the series of Indian Standards on synthetic food colours permitted under thePrevention of Food Adulteration Rules, 1955 issued by the Ministry of Health, Government of India.These rules, inter-alia prescribe:
'All food colours including natural colouring matter and permitted synthetic food colours andtheir preparations or mixtures excluding saffron and curcumin shall be sold only under theDIS Certification Mark.'
'Indian Standard specification for ponceau 4 R' was first issued in 1963. It was revised in 1974 tobring it in line with the FAO/WHO specifications and also taking into account the indigenous datagenerated. This standard is being revised again taking into consideration the latest specification forfood colour issued by FAO/WHO [see FAO Food and Nutrition Paper 31/1 ( 1984 ) 'Specification foridentity and purity of food colours' ], the latest specifications laid down under Food Chemical Codex.EEC Directives, Canadian Food Laws and Food and Drugs Act of USA. In addition, due considerationbas been given to indigenous data.
In this revision the minimum requirement for total dye content has been raised and the maximumrequirement of loss on drying at 135°C and chloride and sulphate expressed as sodium salt has alsobeen increased. The limits for water insoluble matter and combined ether extracts have been mademore stringent although, the requirements have still not been aligned with the International requirements as the industry expressed inability to follow the strict International requirements. TheCommittee, however, decided to collect data on the parameter and review the limits at a laterstage. '
Requirements for Ponceau 4 R, food grade have been prescribed under the PFA Rules. As foodcolours are under compulsory BIS Certification, the modified values given in Table 1 have beenreferred to CCFS for adoption under the PFA Rules. While formulating this standard, necessaryconsideration has been given to the Standards of Weights and Measures (Packaged Commodities)Rules, 1977. The standard is however subject to restrict ions imposed under these Rules, whereverapplicable.
DescriptioD
Common Name - Ponceau 4 R.
Synonyms - C.I. Food Red 7, L·Rot No.4, Coccine Nouvelle, Cochineal Red A; E.E.C. SerialNo. E 124.
Colour of the 0·1 Percent ( m/v ) Solution In Distilled Water - Red.
Colour Index Number ( 1975 ) - No. 16255.
class - Monoazo.
Chemical Name·- Trisodium salt of 1· ( 4· sulpho • 1· naphthylazo ) • 2· naphthol • 6, 8·disulphonic acid.
Empirical Formula - CIOH11N.Ol0S.Na••
Molecular Weight - 604·5.
( Contlnu,d on third cov., )
IS 1558 : 1994
Indian StandardPONCEAU 4R, FOOD GRADE ~ SPECIFICATION
( Second Revision)
2491 :'1972
3.1 The material shall conform to the requirementsprescribed in Table 1.
1 SCOPE
1.1 This standard prescribes the' requirements and themethods ofsamplingandtest forPonceau4R,foodgrade.
Z REFERENCES
The material shall be packed in glass containers,polyethylene container, metal containers, or cardboardcontainers suitably lined with polyethylene. Subject toagreement between the purchaser and the vendor anyother suitable container tnay also be used.
3.2 Freedom fro.. Contaminants
Precautions shallbe taken to ensure that the material isfree from mercury, selenium and cbromimum in anyfonn; aromatic amines, aromatic nitro compounds,aromatic hydrocarbons, and cyanides.3.3 The productshall be processed, packed,stored anddistributed under hygienic conditions in licencedpremises (see IS 2491 : 1972).
4 PACKING AND MARKING
4.1 Packing
Title
Reagentgradewater(thint revision)Methods of sampling and test forfood colours ( secondrevision)Code for hygienic conditions forfood processingunits(first revision)
3 REQUIREMENTS
The followingIndian Standardsare necessary adjunctsto this standard.
IS No.1070: 1992
1699 : 1994
Table 1 Requirements for Ponceau 4R
(Clause 3.1 )
SI No. Characteristic Requirement Method of Test, Ref to
(1)i)
ii)
iii)
iv)
v)
vi)
vii)viii)
ix)
(2)
Total dye content, corrected forsample dried at 105:t 1°C for2 h, percent by mass,Min
Loss on drying at 135°C, percent by mass, Max
andChlorides and sulphatcs expressed as sodium salt, percentby mass, MaxWater-i nsoluble matter, percentby mass, MaxCombined ether extracts percent by mass,MaxSubsidiary dyes, percent bymass,MaxDye intermediates, percent bymass, Max
. Lead,mg/kg,MaxArsenic, mg/kg, MaxHeavy metals, mg/kg, Max
(3)82
18
0.4
0.4
1.0
O.S
103
40
"Annex of ThisStandard
(4)
A
B
c
Clause ofIS 1699 : 1994
(5)
6
1314
7
8
151516
IS 1558 :\1994
4.% Marking
4.%.1 Each container shall be legibly and indeliblymarked with the following information:
a) The words 'FOOD COLOUR';b) Conunon name of tbe colour;c) Cheemical name oftbe colour;d) Colour index number;e) Source of manufacture;t) Date of Inanufacture;g) Nameof major dye intermediates;b) Minimumnet mass in grams or kilograms;j) Batcb or code number;andk) Any other requirements as specified under tbe
The product may also be marked witb tbe StandardMark.
4.2.%.1 The use of the Standard Mark is governed bytheprovisions of the Bureau of Indian Standards Act,1986and tbe Rulesand Regulations made thereunder.The details of conditions under whicb the licence fortbe use of Standard Mark may be granted to manufacturers or producers may be obtained from the Bureauof Iodia'n Standards.
S SAMPLING
S.1 Representative samples of the material for testsshall be drawn and criteria for ascertainingconformityto the rquirements of tbis specificationsball be determined according to the method prescribed in 4 of IS1699: 1994. .
'TESTS. 6.1 Testsshall becarried out as prescribed in col 4 and
5. of Table 1.
6.1 Quality of Reagents
Unless specified otherwise, pure chemicalsand distilledwater (see IS 1070: 1992)shall be employedin tests.
NOTE - 'Pure chemicals"shall mean chemicals that donotcontainimpuritieswhichaffect the resultsof analysis.
ANNEXA
[ Table 1, Item (i) ]
DETERNUNATIONOFTHETOTALDYECONTENT
. A·O GENERAL
Two methods, spectrophotometric metbod and thetitanium trichloride metbod, are specified. In case ofdispute, spectrophotometric method shall be regardedas the reference metbod.
A-I SPECTROPHOTOMETRIC METHOD
A-I.I Apparatus
Suitable spectropbotometer witb properly calibratedscales for botb wavelengtb and optical density. However, a suitable spectrocolorimeter may be used afterproper calibration against a spectrocolorimeter maybe used after proper calibration against aspectrophotometer.
A-I.% ProcedureWeigbaccurately about 250 mg of tbe dye sample anddissolve in 0.1 N hydrochloric acid in 250-mJvolumetric flask. Dilute this further with 0.1 Nhydrochloric acid to make a tinal concentration of 1mg/IOO mJ (approximately). Find out tbe optical density of tbis diluted solution, against 0.1 N bydrochloricacid as blank,.. t 506 nm, in a glass cell witb 10.0 mmlight patb.A-l.2.1 Simultaneously weigb accuratelyabout 2 g of
2
the dye-sample and dry tbis in an air-oven at105:t 1°C for 2 hours. Calculate tbe Joss of mass ondrying; from tbis data, calculate tbe dry mass of tbesample(M)in the finalsolution taken for measurementof opticaldensity. .
A·I.3 Calculation
Totaldye content in tbe sample, percent by mass =ODx 100Mx440
wbereOD = optical density found;M = dry mass of the sample in 100 mJsolution;
and440 = £1%1 em, 506 IUD, for Ponceau4R in 0.1 N
hydrochloricacid.
A·2 TITANIUM TRICHLORIDE METHOD
A-Z.I PROCEDURE
The method given in 5 of IS 1699 : 1994 shall befollowed. The percentage of total dye content sball bedetermined using tbefoJJowing calculations:
1 mJ orO.1 Nn03. 0.015 11 gofPonceau·4R.
IS 2558 : 1994
ANNEX 8
[ Table 1, Item (v) ]
DETERMINATION OF SUBSIDIARY DYE CONTENT
B·I PROCEDURE
The method given in 9 of IS 1699 : 1994 using theconditions given below shall be followed:
DevelopingsolventHeight of ascent of
solvent front
=No.3=17 em, then one hour
for further development.
ANNEX C"
[ Table 1, Item (vi) ]
DETERMINATION OF DYEINTERMEDIATES
e-o Two methods, column chromatographic andascending paper chromatographic method have beenspecified. In case of dispute tbe columnchromatognphic method shall be regarded as thereference method.
c-r METHOD I
C-I.O Principle
Ponceau 4R is prepared by diazotization of I-napthylamine-4- sulphonic acid and tbe diazo so formed iscoupled with 2-naphthol 6 :'8 disulphonic acid. Afterthe completion of tbe reaction the traces of these intermediates remain, which can be estimated by ascendingpaper cbromatography.e-I.1 ApparatusC-I.l.l Chromatogrphy Tank and Ancillary Equipment - as given under 9.2.1 of IS 1699 : 1994.
e-l.t.2 Microsyringe - capable of 0.2 ml with atolerance of:t 0.000 2 mI.C-I.I.3 Ultraviolet Lamp - at 365.5 nm.C·l.1.4 Filter Paper - Whatman No.1 or equivalent.
C·l.Z Reagents
C-I.2.1 Ammonium Hydroxide Solution - sp gr 0.923.
C-l.3.1 Preparation ofSolutionsC-l.3.1.1 Prepare 2 percent mlv solution of the dye ina mixture of nine parts of water and one part of theammonium hydroxide.C-l.3.I.% Reference Substances
a) Prepare on 100 percent basis,mlvsolution of 1Napbtbylantine-4-Sulpbonic Acid in 8 mixtureof nine parts ofwater and one partof ammoniumhydroxide, so that 5 f.ll of the solution shallcorrespond to 1.5 percent in tbe dye sample.
3
b) Prepare on 100 percent basis, mlv solution 'of2-Napbthol 6 : 8 Disulpbonic Acid in a mixtureofoine partsof water and one part of ammoniumhydroxide, so that 5 111 of the solution shallcorrespond to 0.3 percent in the dye sample.
C-l.3.Z Test SubstancesC-I.3.1.1 Dye solution50 ~I of tbe solution sbaII be equivalent to 1 000microrgamsof tbe sample.C-I.3.Z.2 Reference substances
a) I-Naphthylamine-4-Sulphonic Acid, andb) 2-NaphthoI6: 8 Disulphonic Acid.NOlc - Different concentrations of dye intermediatesmay be prepared, if necessary.
C-l.3.3 Mark out a sheet of chromatographic paper asshown in Fig. 3 of IS 1699 : 1994 and spot sufficientquantity of dye sample as unifonnly as possible withthe help of microsyringe. Also spot reference substances (C-l.3.Z.2). Before mounting the sheet of paper, cutotIsome portionoftbe paper below the base line to takecare of any spreading of the initial spot. Pour sufficientdeveloping solvent into the tank to bring the surface ofthe solvent just below the cut portion of tbechromatographic paper. Take out the filter paper afterthe developing solvent has sufficiently moved up. Dryin an oven at 70 to 750 C or by bair drier.C-1.3.4 Detection and Comparison of the DerivativesC-l.3.4.1 Detection and semi-quantitative estimation
Inspect the cbromatogram under ultra violet light.Compare visually the intensity of tbe Ouorescence ofthe sample with tbe reference substance and report tbecontent of l-Naphthylamine-4-Sulphonic Acid, and 2Naphthol6 : 8 Disulpbonic Acid which shall be nearestin intensity to one of the corresponding reference substances.
C-%METH6nz
The column chromatographic method given in toofIS 1699 : 1994 shall be followed.
St~ctu,al For~"'a
OH
N=N
So/ubility - Soluble in water.Sparingly soluble in ethanol.
For the purpose of deciding whether a particular requirement of this standard is complied with, thefinal value, observed or calculated, expressing the result of a test or analysis, sha II be rounded otT inaccordance with IS 2 : 1960 'Rules for rounding off numerical values (revised f. The number ofsignificant places retained in the rounded off value should be the same as that of the specified value inthis standard.
Bareau of Indian Studards
BIS is a statutory institution established under the Bureau oflndianStandardsACI. 1986 to promote harmoniousdevelopment of the activities of standardization.. marking and quality certification of goods and attending toconnected matters in the country.
Copyrigbt
BIS has the copyright ofall its publications. No pan of these publications may be reproduced in any formwithoutthe prior permission in writing of BIS. This does not preclude the free use.. in 'the course of implementing thestandard, of necessary details, such as symbols and sizes, type or grade designations. Enquiries relating tocopyright be addressed to the Director (Publications), BIS.
Review of Indian Standards
Amendmentsare issued to standards as the need arises on the basis of comments. Standards' are also reviewedperiodically; a standard along with amendments is reaffirmed when such review indicates that no changes areneeded: if the review indicates that changes are needed, it is taken up for revision. Users of Indian Standardsshould ascertain that they are in possession of the latest amendments or edition by referring to the latest issueof 'BIS Handbook' and 'Standards: Monthly Additions'.
This Indian Standard has been developed from Doc : No. FAD 8 (257 )
Amendments Issued Since Publication
Amend No. Date of Issue
BUREAU OF INDIAN STANDARDS
Text Affected
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