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Ishrat Mahjabeen, PhD

POSITION: Senior Scientific Officer (since Jan 2014)

Department Of Biosciences

COMSATS Institute of Information Technology, Islamabad

E-MAIL Ishrat.mahjabeen@comsats.edu.pk

PERSONAL DATA

Father’s name Din Muhammad

Address khalid manzil, house # 126, ward #8, mohallah sadaat,

Talagang

Phone number 0092-3135651941, 0092-3333138695

ACADEMIC QUALIFICATION

1. Ph.D., COMSATS Institute of Information Technology Islamabad in 2013.

Major in Cancer Genetics. Thesis title “Studies on Deregulation of Base

Excision Repair Pathway Genes and microRNAs in Head and Neck Cancer”.

2. M.Phil. Quaid-e-Azam University Islamabad in 2006. Major in

Developmental Biology. Thesis title “ Effect of Methylcobalamin on

regeneration of crushed sciatic nerve of Rabbit”.

3. MSc., University of Arid Agriculture Rawalpindi in 2003. Major in

Zoology. Thesis title “Effect of temperature on population of arthropods on

Rosa Indica”.

AREA OF INTRESRT

Cancer Genetics

Molecular Genetics

COURSES TAUGHT

Research Techniques

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RESEARCH EXPERTISE

Light Microscopy and morphometric analysis

Slide Preparation, Semi thin section cutting

DNA and RNA tumor samples and cell lines

Protein extraction from blood samples, tumor samples and cell lines

PCR, Reverse transcriptase PCR and Real time PCR

Sequencing

Mutational analysis, Single stranded confirmation assay, Restriction fragment

length polymorphism

Protein structure prediction

Cell culturing, Cell freezing and cell growth Assay

Cell proliferation assay

Cell migration assay

Cell apoptosis assay

Microarray

MicroRNA profiling and TaqMan Assay

Western Blotting, ELISA

Immunohistochemistry and Flow Cytometry

Training of graduate students and project designing in Lab

COURSES STUDIED

Molecular Biology

Molecular Genetics

Human Genetics

Morphology

Physiology

Biochemistry

Advances in Developmental Biology

Advances in Reproductive Biology

Advances in Human physiology

Evolutionary Biology

Taxonomy

Ecology

Advances in Molecular Biology

Advances in Research

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Research Report

Zoogeography

Andrology

Wild life management

Advances in Report writing

DISTINCTIONS/AWARDS

Gold medal for young scientist 2015 awarded by Pakistan Academy of

Medical Sciences.

Merit based scholarship at Govt Post Graduate college, Rawalpindi during FSc

Merit based scholarship at QAU, Islamabad during M.Phil

PhD, 5000 Indigenous Scholarship from HEC

IRSP (HEC) six monthly Foreign Scholarship

RESEARCH AND WORK EXPERIENCE

2014 to date: Senior Scientific Officer, Department of Biosciences,

COMSATS Institute of Information Technology, Islamabad.

2012- 2014: PhD. Fellow

Cancer Genetics Lab, Department of Biosciences, COMSATS Institute of

Information Technology, Islamabad.

October 2011-April 2012: Research Fellow

Centre College of Dentistry, Center for Molecular Biology of Oral Diseases.

University Of Illinois at Chicago, USA.

2004-2006: Research Fellow

Developmental Biology Lab, Quaid-e-Azam University, Islamabad.

2002-2003: Research Fellow

Entomology Lab, PMAS UAA Rawalpindi.

One year honorary lectureship in Makkah System Of Education Talagang,

(2007-2008)

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STATISTICS AND SOFTWARES

Proficient use of window based software (Ms Word, Ms Excel, Ms

PowerPoint).

Data based software’s operational skills including Statistical software

(Statistica, SPSS, Pc-ORD, and MVSP).

Graph pad prism

Data Assist software for Real Time PCR data analysis

Sequencing software

Protein structure prediction software and other Bioinformatics tools (BLAST,

primer3, ClustalW).

CONFERENCES AND WORKSHOPS

Poster presented at BICMAPE Science Conference, CIIT, Abbottabad. (July

28-29, 2009)

Poster presented at 2nd Annual Biosciences Poster Exhibition and Competition

(BIOPEC), CIIT- Islamabad. (April, 2011).

Poster presented at 1st Annual Biosciences Poster Exhibition and Competition

(BIOPEC), CIIT- Islamabad. (May, 2010).

Sixth International Biennial Conferences of Microbiology, March 18-21,

2007, Quaid-i-Azam University, Islamabad.

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PUBLICATIONS IN INERNATIONAL PEER REVIEWED JOURNALS

1. Mahjabeen I, Ali K, Zhou X, Kayani MA (2014) Deregulation of base excision

repair gene expression and enhanced proliferation in head and neck squamous cell

carcinoma. Tumour Biol. 35(6):5971-83. doi: 10.1007/s13277-014-1792-5.

2. Mahjabeen. I, RM Baig, Sabir M and Kayani MA (2013) Genetic and

Expressional variations of APEX1 are associated with increased risk of head and

neck cancer. Mutagenesis. 28(2):213-218.

3. Mahjabeen I, Baig RM, Masood N, Sabir M, Inayat U, Malik FA, Kayani MA

(2013) Genetic variations in XRCC1 gene in sporadic head and neck cancer

(HNC) patients. Pathology Oncology Research. Res. 19(2):183-8.

4. Mahjabeen I, Chen Z, Zhou X, Kayani MA (2012) Decreased mRNA expression

levels of base excision repair (BER) pathway genes is associated with enhanced

Ki-67 expression in HNSCC. Medical Oncology. 29:3620–3625.

5. Mahjabeen I, Baig RM, Masood N, Sabir M, Inayat U, Malik FA, Kayani MA

(2012) Novel mutations of OGG1 base excision repair pathway gene in laryngeal

cancer patients. Familial Cancer. 11:587–593.

6. Mahjabeen I, Baig RM, Masood N, Sabir M, Malik FA, Kayani MA (2011)

OGG1 Gene Sequence Variation in Head and Neck Cancer Patients in Pakistan.

Asian Pacific J Cancer Prev, 12, 2779-2783.

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7. Chen Z, Jin Y, YU D, Wang A, Mahjabeen I, Wang C, Liu X, Zhou X (2011)

Down-regulation of the microRNA-99 family members in head and neck

squamous cell carcinoma. Oral oncology. 48(8):686-91.

8. Baig RM, Mahjabeen I, Sabir M, Masood N, Ali K, Malik FA, Kayani (2013)

Mutational spectrum of Gelsolin and Its down regulation is associated with breast

cancer. Disease Marker. 1-10.

9. Baig RM, Mahjabeen I, Sabir M, Masood N, Hafeez S, Malik FA, Kayani MA

(2011) Genetic Changes in PTEN Gene and their Association with Breast Cancer

in Pakistan. Asian Pacific Journal of Cancer Prevention. 2365-2370.

10. Sabir M, Baig RM, Mahjabeen I, Kayani MA (2012) Novel germline CDK4

mutations in patients with head and neck cancer. Hereditary cancer in clinical

practice 10:11.

11. Sabir M, Baig RM, Mahjabeen I, Saeed M, Malik FA, Kayani MA (2012)

Rb1/105 gene alterations and head and neck carcinogenesis. Molecular biology

reports 39(10): 9573-81.

12. Sabir M, Baig RM, Mahjabeen I, Kayani MA (2012) Significance of Cyclin B1

polymorphisms in patients with head and neck cancer. Accepted for publication in

Internation journel of biological markers.

13. Masood N, Malik FA, Mahjabeen I, Baig RM , Kayani MA (2011) A novel

CYP1A1 gene polymorphism and the risk of head and neck cancer in Pakistani

population. African Journal of Biotechnology; 10 (27):5273-5280.

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14. Masood N, Kayani MA, Malik FA, Mahjabeen I, Baig RM, Faryal R (2011)

Genetic Variations in Carcinogen Metabolizing Genes Associated with Oral

Cancer in Pakistani Population. Asian Pacific Journal of Cancer Prevention.

12:491-495.

15. Masood N, Mahjabeen I, Malik FA, Baig RM , Kayani MA (2010) Association

of GSTM1 and GSTT1 gene deletions with risk of head and neck cancer in

Pakistan: a case control study. Asian Pacific Journal of Cancer Prevention. 11(4):

881-5.

16. Shakeel S, Mahjabeen I, Kayani MA, Faryal R (2013) Association of SYK

genetic variations with breast cancer pathogenesis. Asian Pacific Journal of

Cancer Prevention 14 (5), 3309-3314.

17. Wahid M, Mahjabeen I, Baig RM, Kayani MA (2013) Expression of CYP1A1

and GSTP1 in human brain tumor tissues in Pakistan. Asian Pac J Cancer

Prev.14(12):7187-91.

18. Mahjabeen I, Kayani MA (2013). Novel Mutations of Apurinic/Apyrimidinic

Endonuclease 1 Gene in Head and Neck Cancer Patients. International Journal of

Clinical Toxicology, 1, 4-8.

19. Sabir M, Baig RM, Ali K, Mahjabeen I, Saeed M, Kayani MA (2014)

Retinoblastoma (RB1) pocket domain mutations and promoter hyper-methylation

in head and neck cancer. Cell Oncol (Dordr). 37(3):203-13.

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20. Bashir N, Sana S, Mahjabeen I, Kayani MA (2014) Association of reduced

XRCC2 expression with lymph node metastasis in breast cancer tissues. Fam

Cancer. DOI: 10.1007/s10689-014-9745-0.

21. Ali K, Mahjabeen I, Sabir M, Baig RM, Faheem M, Kayani MA (2014) Germline

variations of Apurinic/apyrimidinic Endonuclease 1 (APEX1) detected in female

breast cancer Patients. Asian Pacific Journal of Cancer Prevention 15(18):7589-

95.

22. Khan S, Mahjabeen I, Malik FA, Kayani MA (2014) Prognostic Significance of

Altered Blood and Tissue Glutathione levels in Head and Neck cell Carcinoma.

Asian Pacific Journal of Cancer Prevention 15(18):7603-9.

23. Qureshi Z, Mahjabeen I, Baig RM, Kayani MA (2014) Correlation between

selected XRCC2, XRCC3 and RAD51 genes′ polymorphisms and primary breast

cancer in women. Asian Pacific Journal of Cancer Prevention 15 (23): 10225-

10229.

24. Kayani MA, Khan S, Baig RM, Mahjabeen I (2014) Association of RAD 51 135

G/C, 172 G/T and XRCC3 Thr241Met gene polymorphisms with increased risk of

head and neck cancer. Asian Pacific Journal of Cancer Prevention 15 (23), 10457-

10462.

25. Ali K, Mahjabeen I, Sabir M, Mehmood H, Kayani MA (2015) OGG1 Mutations

and Risk of Female Breast Cancer: Meta-Analysis and Experimental Data.

Disease Markers. doi.org/10.1155/2015/690878.

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26. Shah A, Mahjabeen I, Kayani MA (2015) Genetic polymorphisms in cell cycle

regulatory genes CCND1 and CDK4 are associated with susceptibility to breast

cancer. B.U.ON. 20(4):985-93.

27. Akhtar S, Mahjabeen I, Akram Z, Kayani MA (2015) CYP1A1 and GSTP1 gene

variations in breast cancer, A systematic review and case-control study. Familial

cancer. Familial cancer. DOI 10.1007/s10689-015-9849-1.

28. Zafeer M, Mahjabeen I, Kayani MA (2015) Increased expression of ERCC2 gene

in head and neck cancer is associated with aggressive tumors; A systematic review

and case-control study. International Journal of Biological Markers (accepted).

29. Mahjabeen I, Kayani MA (2015) Loss of mitochondrial tumor suppressor genes

expression is associated with unfavorable clinical outcome in head and neck

squamous cell carcinoma: data from retrospective study. Plos One (under-review)

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BOOK CHAPTER

Mahjabeen, Y. Jin, D. Chen, A. Wang, A. Kolokythas, X. Zhou. The role of

microRNA in head and neck cancer. In: Head & Neck Cancer: Current Perspectives,

Advances, and Challenges, (Chapter 33) J.A. Radosevich, ed., ISBN 978-94-007-

5826-1, Springer (2013).

RESEARCH SUPERVISION

Graduates students supervised

Saman Jahan Ansari (FA-11-BBS-012). Thesis title “Studies on OGG1

polymorphism (ser326cys) in Thyroid Cancer Patients”.

Graduates students co-supervised

AbidUllah Khan (FA-12-RBS-001). Thesis title “Analysis of CCND1 and

CDK4 variations in Breast Cancer Patients in Pakistani Population”.

Sumaira Akhtar (FA-12-RBS-023). Thesis title “Analysis of Glutathione S-

transferase PI and CYP1A1 variations in Breast cancer patients”.

Asad Ullah Khan (FA12-RBS-003). Thesis title “Analysis of CYP2A6 and

CYP2D6 genes in Head and Neck Cancer”.

Malik Waqar Ahmed (FA13-RBM-006). Thesis title “Studies on expression of

PTEN and proliferation marker Ki-67 in patients with head and neck cancer”.

Maryam Zafeer (FA13-RMG-002). Thesis title “Expression deregulation of

XPD/ERCC2 gene and proliferation marker Ki-67 in patients with head and

neck cancer”.

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Project Awarded

“Analysis of XRCC1 Gene in Cancer Patients with HNC” (0.2 millions) By

CIIT (2012-2013). Dr Ishrat Mahjabeen, Dr Mahmood Akhtar Kayani.

Genetic, Epigenetic and Functional Analysis of Base Excision Repair

Pathway Genes in Thyroid Cancer Patient. (3.1 millions) NRPU project

HEC (2015-2017) Dr Mahmood Akhtar Kayani, Dr Ishrat Mahjabeen.

Arsenic Induced Genotoxicity in workers of different Industries of Pakistan

(3.5 millions) NRPU project HEC (2015-2017) Dr Zertashia Akram, Dr

Ishrat Mahjabeen.

Role Of microRNA-145 in Regulation of Metastasis in Head And Neck

Cancer by Targeting Magnesium Superoxide Dismutase 2 (SOD2) (1.0

millions) ISESCO Joint Research Grant Program Morocco (under review)

Dr Ishrat Mahjabeen.

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REFERENCES

1. Dr. Mahmood Akhtar Kayani, Professor, Department of Biosciences,

COMSATS Institute of Information Technology Islamabad.

E-mail: mkayani@comsats.edu.pk.

Telephone #: 0092-321-5357981.

2. Dr. Xiaofeng Zhou, Associate Professor, Center for Molecular Biology of Oral

Diseases, University of Illinois at Chicago, USA.

E-mail: xfzhou@uic.edu

Telephone #: (312) 355-0825

.