1

Supplementary Information  

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Supplementary Figure S1 Scatter plots of alpha diversity indices versus spring

temperature and pH. (a) Shannon diversity index vs. temperature, (b) Shannon diversity

index vs. pH, (c) Chao1 vs. temperature, (d) Chao1 vs. pH, (e) Faith’s Phylogenetic

distance vs. temperature, (f) Faith’s Phylogenetic distance vs. pH, (g) OTUs vs.

temperature and (h) OTUs vs. pH. Solid lines represent the best fit Gaussian 4 parameter

model to the data. Dashed lines represent the best fit linear model to the data. Diversity

indices were calculated using 2,730 sequences per spring sample. Symbol shape indicates

Canada ( ) or New Zealand ( ).

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Supplementary Figure S2 Alpha diversity indices versus spring temperature at different

OTU clustering levels. (a) OTUs vs. temperature, sequences clustered at 95 %, (b) OTUs

vs. temperature, sequences clustered at 90 %, (c) Shannon diversity index vs.

temperature, sequences clustered at 95 %, and (d) Shannon diversity index vs.

temperature, sequences clustered at 90 %. Data calculated at a clustering level of 97% are

shown in Supplementary Figure S1. Solid lines represent the best fit Gaussian 4

parameter model to the data. Diversity indices were calculated using 2,730 sequences per

spring sample. Symbol shape indicates Canada ( ) or New Zealand ( ).

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Supplementary Figure S3 Alpha diversity indices versus spring temperature after data

filtering for singletons. (a) OTUs vs. temperature, singletons removed, (b) OTUs vs.

temperature, singletons and reads only present in one sample in the dataset removed, (c)

Shannon diversity index vs. temperature, singletons removed, (d) Shannon diversity

index vs. temperature, singletons and reads only present in one sample in the data set

removed. Solid lines represent the best fit Gaussian 4 parameter model to the data.

Diversity indices were calculated using 2,730 sequences per spring sample. Symbol

shape indicates Canada ( ) or New Zealand ( ). Unfiltered data showing the same

patterns are shown in Supplementary Figure S1.

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Supplementary Figure S4 Total OTUs detected (a) and Shannon diversity index (b)

versus spring temperature for data that were denoised using the QIIME pipeline. Solid

lines represent the best fit Gaussian 4 parameter model to the data. Diversity indices were

calculated using 2,730 sequences per spring sample. Symbol shape indicates Canada ( )

or New Zealand ( ). For comparison, data that were not denoised are presented in

Supplementary Figure S1.The diversity-temperature relationship showed the same strong

monotonic pattern in both datasets, and the R2 values of 0.40 for the Shannon index and

0.47 for OTU richness in the denoised dataset were similar to the respective values of

0.40 and 0.44 in the unfiltered dataset.

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Supplementary Figure S5 Number of OTUs versus temperature range rarefied to a

depth of 35,000 sequences showing a decrease in total OTUs with an increase in

temperature. OTUs were clustered at 97 % ( ), 95 % ( ), 90 % ( ) similarity and 97 %

similarity with singletons removed ( ).

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Supplementary Figure S6 Scatter plot of Shannon diversity index versus spring latitude

from equator of the microbial communities in neutral-alkaline springs (pH > 5). Diversity

indices were calculated using 2,730 sequences per spring sample. Symbol shape indicates

Canada ( ) or New Zealand ( ).

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Supplementary Figure S7 Nonmetric multidimensional scaling plot of geothermal

spring microbial communities based on unweighted UniFrac showing separation of sites

based on temperature and pH. Colour coding indicates temperature (a) or pH (b).

Symbols in (b) indicate location: Canada ( ) or New Zealand ( ).

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Supplementary Figure S8 Relative abundances of bacterial and archaeal phyla versus

temperature range (a) and pH range (b). Phyla with abundances >1 % in any range are

shown, low abundance phyla are included in the ‘other’ category.

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Supplementary Table S1. Sample ID, pH, temperature and associated diversity indices. Sample ID includes year of sampling. Diversity indices were calculated at a depth of 2,730 sequences.  

Sample ID pH Temperature (°C) Location Chao1 Phylogenetic

Distance

Degrees Latitude from

Equator BC3_2009 6.86 13.4 Canada 2315 96.28 49 BC4_2012 8.68 31.8 Canada 2957 97.59 49 BC5_2012 8.67 22.4 Canada 3435 103.22 49 BCs1_2009 6.5 30 Canada 2800 96.72 49 BCs2_2009 7.05 30 Canada 3124 99.65 49 BCse1_2009 7.82 32.4 Canada 1691 81.35 49 BMS_2012 6.93 37.4 Canada 999 52.23 51 BR2_2011 7.32 51.7 Canada 315 26.14 53 BR3_2011 7.32 51.7 Canada 397 27.81 53 DC1_2010 8.15 43.6 Canada 1867 72.11 49 DC1_2012 8.15 41.3 Canada 1128 62.13 49 DC10_2010 8 26.8 Canada 3128 103.14 49 DC11_2010 8 26.8 Canada 1119 49.78 49 DC12_2010 8 28.7 Canada 2680 97.15 49 DC14_2010 8 45 Canada 752 38.09 49 DC15_2010 8 27.2 Canada 581 32.11 49 DC15_2011 8 43.6 Canada 1312 56.70 49 DC16B_2010 8.15 44.5 Canada 1424 67.28 49 DC2_2010 7.08 79.9 Canada 218 10.90 49 DC2_2012 7.93 66.4 Canada 286 17.35 49 DC4_2010 7.08 85.9 Canada 240 8.92 49 DC5_2010 7.08 85.9 Canada 262 11.15 49 DC6_2010 7.46 77.4 Canada 359 23.74 49 DC6_2012 8.50 44.7 Canada 451 28.39 49 DC7_2010 8.15 35.3 Canada 1355 55.52 49 DC7_2012 8.5 44.7 Canada 392 33.62 49 DC8_2010 7.94 62.8 Canada 468 28.56 49 DC8_2012 7.94 64.7 Canada 298 23.74 49 DC9_2012 7.94 64.7 Canada 341 21.12 49 DCm_2010 8.3 54.9 Canada 1290 58.31 49 DCm_2011 8.3 43.6 Canada 865 45.76 49 DCs6_2011 7.46 77.4 Canada 318 15.39 49 DCsB_2011 8 27.2 Canada 555 33.13 49 DCsGBL_2011 8.15 37.9 Canada 2119 89.97 49 DCm2_2011 8.3 45 Canada ND ND 49 Deer1_2012 7.81 30.0 Canada 1937 92.56 59 Deer2_2012 7.81 30.0 Canada 1254 56.49 59 Deer6_2012 7.81 30.0 Canada 1662 63.21 59 FM4_2012 6.71 38.5 Canada 676 45.81 50 FM6_2012 6.17 44.7 Canada 1647 65.23 50 FMs_2009 6.51 39.5 Canada 1596 62.75 50 FMs2_2009 6.87 39.5 Canada 1451 63.74 50

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FS1_2010 7.2 24 Canada 2543 105.18 49 FS1_2012 7.48 21.2 Canada 2625 109.26 49 FS2_2010 7.2 19.2 Canada 2972 97.82 49 FS3_2012 7.48 21.2 Canada 2634 106.32 49 FS4_2012 7.48 21.2 Canada 1964 79.62 49 FS5_2010 7.2 24 Canada 3243 102.88 49 FS7_2010 7.2 24 Canada 2499 96.90 49 FS8_2012 7.67 21.0 Canada 800 42.03 49 FS9_2012 7.18 20.5 Canada 2261 100.15 49 FZ1_2012 8.01 40.3 Canada 1020 57.00 54 FZ2_2012 8.01 40.3 Canada 1447 51.22 54 GH3_2011 6.86 57.2 Canada 893 43.92 53 GS1_2012 7.02 56.1 Canada 492 25.53 53 GS3_2012 7.19 54.4 Canada 351 20.49 59 GS4_2012 7.3 28.9 Canada 1357 72.87 59 GS6_2012 7.19 54.4 Canada 501 29.26 59 GS7_2012 7.19 51 Canada 433 26.26 59 GS8_2012 6.81 56.2 Canada 180 12.34 59 HS1_2012 8.1 67 Canada 337 22.05 54 IR1_2012 7.87 7.5 Canada 1126 44.37 81 IR2_2012 7.87 7.5 Canada 585 28.28 81 IR4a_2012 7.87 7.5 Canada 737 40.99 81 IR4b_2012 7.87 7.5 Canada 1017 55.31 81 IR4c_2012 7.87 7.5 Canada 837 41.70 81 IR4d_2012 7.87 7.5 Canada 897 44.81 81 KP1_2012 7.79 85.8 Canada ND ND 54 KP3_2012 7.79 54 Canada 935 44.98 54 LL1_2011 8.27 45.1 Canada 632 36.82 54 LL1_2012 8.2 10 Canada 3211 109.64 54 LL2_2012 8.2 51.1 Canada 493 21.78 54 LL3_2011 8.27 45.1 Canada 668 37.41 54 LL4_2012 8.2 51.1 Canada 573 36.30 54 LL5_2012 8.2 51.1 Canada 600 36.05 54 LL6_2012 8.2 49.5 Canada ND ND 54 LN1_2012 6.85 50.4 Canada 397 23.10 60 LN2_2012 6.85 50.4 Canada 455 28.98 60 LN4_2012 7.16 33.1 Canada 1907 75.01 60 LN5_2012 6.85 50.4 Canada 805 43.59 60 LOR12_2010 3.21 48.5 New Zealand 173 10.71 38 LOR16_2010 5.02 29.7 New Zealand 366 19.74 38 LOR17_2010 5.97 60.6 New Zealand 214 13.89 38 LOR20_2010 4.72 70.1 New Zealand 699 37.25 38 LOR21_2010 2.64 22.5 New Zealand 249 11.92 38 LOR4_2010 6.62 79.8 New Zealand 339 16.20 38 LOR7_2010 3.09 66.5 New Zealand 188 8.61 38 LR1_2012 6.76 53.5 Canada 2890 104.44 59 LR2_2012 6.76 53.5 Canada 512 30.63 59 LR6_2012 6.76 53.5 Canada 426 24.53 59 LS1_2012 7.25 42.3 Canada 1380 68.17 60

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LS2_2012 7.25 42.3 Canada 742 42.81 60 LS3_2012 7.25 42.3 Canada 1999 85.15 60 LS4_2012 7.25 42.3 Canada 848 43.75 60 LS8_2012 7.25 42.3 Canada 790 43.81 60 LSS_2009 8.63 20 Canada 1628 72.18 50 LSS2_2012 7.15 41.5 Canada 1352 66.46 50 LSS3_2012 7.05 42.4 Canada 984 60.65 50 MM1_2010 7.94 28.9 Canada 1116 63.31 50 MM2_2010 7.94 27.5 Canada 1373 65.92 50 MM2_2012 7.29 34.9 Canada 2066 72.39 50 MM3_2010 7.94 21.6 Canada 2231 72.65 50 MMm_2010 7.94 27.5 Canada 1114 59.31 50 MT1_2011 6.87 46 Canada 374 25.67 53 MT2_2011 6.87 46 Canada 650 34.12 53 NGM1_2010 6.98 45 New Zealand 1043 53.00 38 NGM2_2010 6.94 45 New Zealand 1325 71.85 38 NGM4_2010 7.31 45 New Zealand 1553 80.28 38 NGM5_2010 6.99 50 New Zealand 680 41.64 38 NGM51_2012 6.99 50 New Zealand 452 23.32 38 NGM52_2012 6.99 50 New Zealand 505 35.47 38 NGM53_2012 6.99 50 New Zealand 733 48.32 38 NGM6_2010 6.9 56 New Zealand 674 33.87 38 NGM7_2010 7.19 50 New Zealand 1044 49.04 38 NGM7_2012 7.19 50 New Zealand 528 28.47 38 NGM72_2010 7.19 50 New Zealand 995 60.52 38 NGM89_2012 7.9 88.8 New Zealand 372 13.60 38 NGM95_2010 7.9 94.7 New Zealand 250 9.78 38 NGW01_2011 3 40.5 New Zealand ND ND 35 NGW02_2011 3.2 40.3 New Zealand 611 30.10 35 NGW03_2011 3.5 44.9 New Zealand 103 3.46 35 PB1_2012 6.34 45.9 Canada 604 39.84 59 PB5_2012 6.39 31.9 Canada 1284 66.47 59 Pool2_2012 7.07 31.8 Canada 1410 57.59 60 PPMP_2011 3.36 24.2 Canada 1554 69.38 51 PPOM_2009 3.36 24.2 Canada 1367 71.55 51 PR4_2012 7.54 31.3 Canada 1666 87.69 57 PR5_2012 7.33 32.9 Canada 925 40.60 57 PR7_2012 7.38 29.3 Canada 2726 109.48 57 RC1_2009 7.5 25 Canada 2739 86.75 50 RC1_2012 7.61 36.7 Canada 2464 93.50 50 RC3_2012 7.62 36.4 Canada 1852 86.81 50 RC4_2012 7.47 37.9 Canada 2169 91.63 50 RCs1_2009 7.5 30 Canada 3235 102.21 50 RR2_2009 7.14 17.9 Canada 1926 72.71 50 RRS1_2009 7.6 20 Canada 4594 118.91 50 RRW_2009 6.49 20 Canada 1453 64.76 50 RTK10_2010 2.82 31.4 New Zealand 235 15.25 38 RTK12_2010 2.5 78.1 New Zealand 117 9.75 38 RTK2_2010 2.31 35.2 New Zealand 193 7.40 38

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RTK3_2010 2.35 40.1 New Zealand 133 5.78 38 RTK4_2010 2.36 46.9 New Zealand 166 6.36 38 RTK5_2010 2.11 54.5 New Zealand 250 9.41 38 RTK6_2010 1.83 64.5 New Zealand 269 9.31 38 RTK7_2010 2.08 68.7 New Zealand ND ND 38 RTK9_2010 2.57 25 New Zealand 282 12.11 38 SW3_2011 7.49 23.9 Canada 1030 52.73 53 TIK2_2010 6.32 60 New Zealand 410 41.08 38 TIK3_2010 2.58 36.8 New Zealand 323 14.63 38 TIK4_2010 3.38 44.1 New Zealand 217 9.93 38 TIK4_2012 3.2 44.1 New Zealand 291 13.63 38 TIK5_2012 3.5 58.2 New Zealand 226 9.25 38 TIK6_2012 3.5 58.2 New Zealand 254 11.13 38 TIK7_2012 3.5 70.9 New Zealand 392 19.59 38 TIK8_2012 3.9 81.6 New Zealand 283 14.83 38 Toad2_2012 6.61 50.0 Canada 726 37.61 58 VM2_2012 7 21.2 Canada 354 17.72 51 WH1_2010 7.43 31.8 Canada 1738 62.76 49 WH4_2010 7.43 29.1 Canada 2277 85.16 49 WH5_2010 7.43 28.7 Canada 2219 83.72 49 WH7_2010 7.43 27.4 Canada 3006 108.94 49 WKT4_2010 7.52 99 New Zealand 378 21.06 38 WKT6_2010 8.96 81.6 New Zealand 420 16.82 38 WKT7_2010 7.25 81.6 New Zealand 537 25.35 38 WKT8_2010 7.81 94.5 New Zealand 277 11.14 38

ND: Not Determined  

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Supplementary Table S2. OTUs and Shannon diversity indices calculated with various quality filtering steps. Sample ID includes year of sampling. Diversity indices were calculated at a depth of 2,730 sequences.

Sample ID Unfiltered Singletons removed

Singletons and reads only present in one

sample removed Denoised

Shannon OTUs Shannon OTUs Shannon OTUs Shannon OTUs BC3_2009 7.99 914 7.70 772 7.36 677 7.42 599 BC4_2012 9.74 1343 9.59 1216 9.51 1172 8.42 646 BC5_2012 9.74 1368 9.51 1205 9.41 1139 8.52 675 BCs1_2009 8.88 1119 8.58 954 8.51 914 7.99 636 BCs2_2009 9.66 1273 9.46 1122 9.37 1058 8.62 709 BCse1_2009 7.82 785 7.54 648 7.39 603 7.30 550 BMS_2012 6.92 495 6.70 397 6.07 280 6.13 313 BR2_2011 3.51 192 3.45 177 3.45 178 2.98 124 BR3_2011 5.11 224 5.00 191 4.80 180 4.76 144 DC1_2010 8.12 707 7.90 588 7.80 552 7.57 446 DC1_2012 7.59 527 7.46 460 7.43 444 7.15 352 DC10_2010 8.95 1115 8.64 936 8.51 885 8.18 670 DC11_2010 6.00 482 5.70 368 5.29 318 5.82 326 DC12_2010 9.03 1045 8.77 886 8.70 844 8.33 636 DC14_2010 4.98 296 4.85 237 4.77 215 4.71 191 DC15_2010 4.14 275 4.02 228 3.86 214 3.86 191 DC15_2011 6.84 561 6.62 464 6.55 436 6.29 337 DC16B_2010 7.43 591 7.23 492 7.16 473 7.05 397 DC2_2010 3.35 88 3.29 72 3.27 65 3.08 50 DC2_2012 4.08 156 3.97 127 3.93 117 3.51 82 DC4_2010 2.78 94 2.74 73 2.60 58 2.04 42 DC5_2010 3.30 103 3.25 83 3.18 70 2.81 50 DC6_2010 3.61 160 3.55 142 3.55 139 3.38 118 DC6_2012 4.23 207 4.12 169 3.99 150 4.03 140 DC7_2010 6.44 536 6.26 455 6.15 427 5.94 336 DC7_2012 5.35 221 5.29 198 5.27 192 5.18 164 DC8_2010 4.34 213 4.24 182 4.19 167 4.10 147 DC8_2012 4.84 175 4.82 166 4.78 162 4.34 117 DC9_2012 3.13 179 3.01 149 2.96 139 2.82 103 DCm_2010 6.83 522 6.62 429 6.58 415 6.34 330 DCm_2011 5.88 405 5.76 353 5.65 322 5.40 254 DCs6_2011 3.57 126 3.51 106 3.47 95 3.25 72 DCsB_2011 5.58 283 5.44 237 5.14 208 5.32 212 DCsGBL_2011 8.70 953 8.57 873 8.47 821 8.04 608 DDm_2011 ND ND ND ND ND ND ND ND Deer1_2012 8.65 929 8.20 632 8.03 592 7.77 596 Deer2_2012 6.68 530 6.51 447 6.64 435 6.24 368 Deer6_2012 7.54 651 7.30 540 6.93 425 6.68 410 FM4_2012 6.40 352 6.29 311 6.19 286 6.15 261 FM6_2012 7.85 742 7.65 635 7.29 570 6.94 433 FMs_2009 7.72 632 7.47 510 7.38 477 7.21 413

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FMs2_2009 7.31 584 7.02 454 6.89 425 6.78 376 FS1_2010 8.84 1045 8.50 837 8.30 774 8.08 703 FS1_2012 8.57 1082 8.29 936 8.25 911 7.98 754 FS2_2010 9.48 1211 9.24 1046 9.15 995 8.35 643 FS3_2012 8.73 1076 8.55 965 8.48 931 8.13 720 FS4_2012 7.04 817 6.81 722 6.61 661 6.50 551 FS5_2010 8.62 1157 8.39 1021 8.28 962 7.93 729 FS7_2010 8.50 972 8.22 815 7.98 717 7.68 624 FS8_2012 4.31 341 4.16 291 3.89 252 3.18 240 FS9_2012 8.37 997 8.09 845 7.90 802 7.63 668 FZ1_2012 7.23 527 7.08 452 6.95 427 6.58 344 FZ2_2012 7.51 678 7.30 568 6.93 473 6.26 307 GH3_2011 5.04 401 4.71 287 2.47 113 4.64 252 GS1_2012 4.78 258 4.56 197 4.17 145 4.04 135 GS3_2012 4.15 155 4.03 121 4.02 111 3.90 91 GS4_2012 7.76 629 7.56 530 7.44 501 7.32 435 GS6_2012 4.62 227 4.48 185 4.40 168 4.18 119 GS7_2012 3.50 184 3.37 151 3.31 135 3.08 108 GS8_2012 1.72 80 1.67 67 1.54 45 1.41 37 HS1_2012 4.06 170 3.99 146 3.68 128 3.64 94 IR1_2012 7.18 538 6.99 446 6.61 392 5.50 250 IR2_2012 4.88 283 4.74 233 4.53 199 4.55 164 IR4a_2012 6.57 404 6.41 337 6.29 311 6.06 246 IR4b_2012 7.39 533 7.23 454 7.14 419 6.93 360 IR4c_2012 6.54 376 6.38 318 6.36 301 6.22 272 IR4d_2012 7.05 465 6.87 397 6.69 354 6.57 315 KP1_2012 ND ND ND ND ND ND ND ND KP3_2012 6.39 399 6.20 323 6.13 299 5.80 239 LL1_2011 5.95 310 5.82 267 5.81 258 5.41 176 LL1_2012 9.59 1295 9.39 1140 9.16 1030 8.61 722 LL2_2012 4.83 223 4.71 184 4.64 163 4.14 104 LL3_2011 5.63 309 5.52 265 5.42 236 5.10 172 LL4_2012 5.97 299 5.89 260 5.86 249 5.49 186 LL5_2012 5.89 290 5.78 247 5.73 231 5.15 168 LL6_2012 ND ND ND ND ND ND ND ND LN1_2012 2.64 188 2.54 161 2.37 131 2.31 116 LN2_2012 3.65 219 3.52 183 3.45 168 3.35 144 LN4_2012 8.43 838 8.19 711 8.09 670 7.53 448 LN5_2012 6.09 405 5.96 353 5.89 337 5.41 214 LOR12_2010 3.41 96 3.33 76 3.25 62 3.08 41 LOR16_2010 4.73 164 4.63 132 4.18 107 4.17 85 LOR17_2010 2.18 98 2.12 78 2.02 68 1.69 49 LOR20_2010 5.63 315 5.54 268 5.37 240 5.12 202 LOR21_2010 2.92 118 2.83 94 2.73 83 2.37 50 LOR4_2010 4.70 160 4.57 117 4.43 94 4.14 65 LOR7_2010 2.65 73 2.59 57 2.60 56 2.28 30 LR1_2012 8.96 1136 8.77 999 8.59 914 8.11 663 LR2_2012 4.87 233 4.75 196 4.67 176 4.58 150 LR6_2012 3.33 177 3.25 149 3.16 130 3.05 112

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LS1_2012 8.10 683 7.94 591 7.74 534 7.33 448 LS2_2012 6.61 376 6.47 318 6.41 296 6.01 242 LS3_2012 8.21 892 8.00 774 7.78 713 7.56 537 LS4_2012 6.33 398 6.25 349 6.23 338 6.02 263 LS8_2012 6.42 379 6.29 323 6.25 311 6.17 261 LSS_2009 7.77 693 7.66 631 7.53 594 7.34 498 LSS2_2012 7.78 617 7.66 545 7.55 508 7.32 430 LSS3_2012 7.70 576 7.60 516 7.51 488 6.91 349 MM1_2010 7.11 607 7.00 548 6.89 519 6.67 416 MM2_2010 6.49 650 6.23 554 6.09 521 6.02 415 MM2_2012 8.66 955 8.45 828 8.32 773 7.56 481 MM3_2010 8.49 877 8.20 719 8.11 675 7.59 454 MMm_2010 7.09 513 6.96 449 6.85 409 6.74 361 MT1_2011 4.62 200 4.49 161 4.40 147 4.36 129 MT2_2011 5.27 309 5.09 252 5.04 224 4.91 184 NGM1_2010 6.32 452 6.13 370 6.05 352 5.86 276 NGM2_2010 7.56 611 7.34 510 7.24 466 6.90 392 NGM4_2010 7.51 712 7.31 618 7.20 594 6.49 439 NGM5_2010 6.40 360 6.33 318 6.27 303 5.51 230 NGM51_2012 5.09 233 5.00 200 4.97 195 3.75 118 NGM52_2012 6.11 289 6.06 254 5.94 241 5.39 175 NGM53_2012 6.44 377 6.35 336 6.23 314 5.91 247 NGM6_2010 5.76 292 5.62 237 5.59 226 4.88 162 NGM7_2010 6.56 450 6.32 358 6.19 309 6.00 248 NGM7_2012 5.39 269 5.30 235 5.31 234 4.26 139 NGM72_2010 7.57 561 7.48 507 7.25 454 7.06 391 NGM89_2012 4.45 171 4.29 122 4.19 104 3.60 38 NGM95_2010 3.53 134 3.40 98 3.37 88 2.67 20 NGW01_2011 ND ND ND ND ND ND ND ND NGW02_2011 3.32 187 3.24 165 3.23 157 2.89 130 NGW03_2011 1.17 38 1.15 29 1.12 25 0.63 14 PB1_2012 5.86 324 5.72 268 5.60 244 5.39 212 PB5_2012 6.65 583 6.52 507 6.44 475 6.20 405 Pool2_2012 6.82 549 6.61 450 6.33 383 5.83 359 PPMP_2011 8.22 756 8.07 659 7.63 547 7.33 425 PPOM_2009 7.86 676 7.68 579 7.52 539 7.23 443 PR4_2012 7.85 755 7.65 665 7.53 622 7.17 556 PR5_2012 5.62 339 5.44 269 5.17 228 5.21 209 PR7_2012 8.95 1085 8.75 950 8.53 864 8.10 721 RC1_2009 8.87 1102 8.61 944 8.56 916 7.68 546 RC1_2012 9.05 1080 8.88 970 8.83 937 8.22 640 RC3_2012 7.92 864 7.75 777 7.53 706 7.34 587 RC4_2012 8.56 964 8.39 856 8.26 803 7.98 610 RCs1_2009 9.40 1252 9.20 1115 9.13 1067 8.44 711 RR2_2009 7.43 757 7.20 653 7.14 631 6.59 461 RRS1_2009 10.05 1551 9.68 1258 9.59 1209 8.51 719 RRW_2009 7.19 684 6.91 552 6.76 514 6.01 365 RTK10_2010 3.58 125 3.53 109 3.50 103 3.36 82 RTK12_2010 0.66 65 0.64 61 0.62 55 0.48 39

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RTK2_2010 3.97 98 3.93 84 3.92 77 3.21 27 RTK3_2010 2.62 77 2.59 65 2.58 65 2.21 21 RTK4_2010 2.61 95 2.58 86 2.57 83 2.05 30 RTK5_2010 4.32 129 4.25 108 4.26 106 3.19 39 RTK6_2010 4.58 153 4.51 132 4.45 117 3.34 45 RTK7_2010 ND ND ND ND ND ND ND ND RTK9_2010 3.44 133 3.36 102 3.11 72 2.53 41 SW3_2011 6.20 500 6.02 426 5.84 384 5.69 306 TIK2_2010 7.02 310 6.97 292 6.90 276 6.68 239 TIK3_2010 4.13 164 4.07 140 3.93 115 3.46 66 TIK4_2010 3.46 111 3.41 98 3.38 92 2.87 41 TIK4_2012 3.79 133 3.72 109 3.68 101 3.47 63 TIK5_2012 2.94 118 2.87 103 2.88 99 2.46 38 TIK6_2012 3.34 138 3.28 118 3.25 109 2.74 44 TIK7_2012 4.71 221 4.62 190 4.59 184 4.07 86 TIK8_2012 4.08 157 3.98 132 3.90 118 3.01 64 Toad2_2012 4.85 292 4.69 245 4.32 205 4.57 206 VM2_2012 3.47 146 3.38 112 3.05 76 2.66 80 WH1_2010 7.54 696 7.23 554 7.13 517 6.54 372 WH4_2010 8.76 951 8.54 812 8.38 748 7.94 565 WH5_2010 7.91 909 7.59 764 7.55 742 7.19 536 WH7_2010 8.89 1187 8.61 1013 8.48 957 8.05 707 WKT4_2010 3.71 188 3.56 143 3.43 127 3.08 76 WKT6_2010 4.62 184 4.51 140 4.45 122 3.84 68 WKT7_2010 4.99 242 4.83 192 4.44 130 4.33 117 WKT8_2010 2.36 132 2.27 102 2.10 82 1.38 34 ND: Not Determined