Blok 9Dinamika Biologi MolekulerPendahuluanMolecular BiologyThe study of biology at a molecular levelOverlaps with other areas of biology and chemistry particularly genetics and biochemistryMolecular Biology!oncerns itself with understanding the interactions between the various systems of a cell including the interactions between D"# $"# and protein biosynthesis as well as learning how these interactions are regulated"eeds established methods%techni&ues for monitoring each element and processLink between Molecular Biology, Biochemistry and GeneticFunction of GenesFunction of GenesControl the physical development, behavior, and being of organisms (look, size, health, etc)Genes encode the information necessary to construct the chemicals (proteins, etc.) needed for the organism to functionApplication of Molecular Biology'( ) '(( mHow DNA is How DNA is packed in packed in chromosome?chromosome?(animation) (animation)Are their genome the same?Are their genome the same?Almost all (>99.5%) of human genome are the same *ingle base se&uence variation among individuals *ome *"Ps are ethnic specific and some show fre&uency variation among different populations # stable polymorphism fre&uently associated with disease susceptibility drug response etc

'+ !%!,+ !%T-+ T%TSingle Nucleotide Polmorphism Single Nucleotide Polmorphism (SNP)(SNP) (animation) (animation)SNPs !dentificationSNPs !dentificationT%! .%!DNA Finger PrintDNA Finger PrintApplication of DNA Finger PrintApplication of DNA Finger PrintCriminalPaternity testVariations and Mutations:There are aboutmillion locations !here single"base #$% di&erences (single nucleotide polimorphims, '$(s) occur in humans'ome '$(s are ethnic speci)c and some sho! fre*uency variation among di&erent populations% stable polymorphism fre*uently associated !ith disease susceptibility, drug response, etc"here are the differences?"here are the differences?'+ !%!,+ !%T-+ T%TMateri /uliahPendahuluan Blok 9.en prokariot dan gen eukariot!entral dogma 0 replikasi 12sruktur 3 fungsi D"#4 Pengantar "utrigenomik!entral dogma0 transkripsi dan translasiPasca translasi 0 5b sebagai salah satu contoh*ingle gene mutation diseasePatobiologi siklus sel 2 karsinogenesisD"# analysis 0 isolasi dan purifikasiMutasi dan D"# $epair#plikasi biomol 0 resistensi mikrobaPrinsip dan teknik isolasi protein#plikasi/linis 0 stem cell#plikasi /linis0 Pharmacogenomics polimorfismsPB' 6solasi 3 Purifikasi D"# 17'4PB' 6solasi 3 Purifikasi D"# 17,4 Terap gen dan D"# finger printingD"# analysis 0P!$D"# analysis 0 sekuens D"#D"# analysis 0 restriction en8yme 3 $97PProtein analysis 1:76*# ;estern Blot dll4$ekayasa genetic 0antibody monoclonal immunhistokimia transgenic animal$ekayasa genetic 0 !loningPraktikum'+ 6solasi dan purifikasi D"#,+ P!$-+ :lectrophoresis