DYNAMIC ELECTROPORATION OF

WATER

Jacob Frye, Courtney Green, Christopher Robertson, and Scott Shields

Introduction

Water is the most important resource.

chemicals(chlorine, iodine) are widely used for sterilization.

Development of alternative sterilization using electricity.

Background

Electroporation – forcefully opening pores of bacteria.

Electrical team performed static electroporation tests.

Results more inclined towards repetition and low voltage.

Background cont.

Use of E. coli k12 to minimize risk while properly modeling bacteria response to electroporation.

Group certification to handle high voltage sources.

Escherichia coli K12

Escherichia coli K12

Safe and Harmless. Similar Properties to Common or Lethal

E. Coli Optima Growth at 37 degrees Celsius Kill rate exists at 42 degrees Celsius

Biosafety Qualifications

To work in the biological lab, Scott and Jacob had to pass the biosafety lab examination and read the Biosafety lab manual

Strict guidelines for the type of clothing and to properly test E. coli K12 around other dangerous bacteria.

Grow Process of E. coli K12

Plate preparation Growing process Incubation period Treatment and testing Growing treated bacteria Determine bacterial reduction

Specific Phases of E. coli K12

Lag Phase Log/Exponential Phase Stationary Phase Death Phase

Design Parameters

Use results from previous electrical team to develop mechanical flow system.

100% kill rate of bacteria 5 kV/cm³ to kill bacteria Attain an 8 oz. cup of drinkable water in

under 10 minutes

Figure 1: Primary Design of Fluid Chamber

Figure 2: Secondary Design (Implementation Pending Time Constraints)

Figure 3: Peristaltic Pump used to generate flow

Manufacturing

Prefabricated Acrylic Rectangle Tubing Lathe turned connectors 16 gauge silver sheet 8 gauge silver rod lathe turned ends Interference press fit connection Silicon adhesive for water containment

Figure 4: Fluid Chamber with silver plates connected to Peristaltic Pump

Preliminary Testing

Determine the possible flow rates with the Peristaltic Pump.

Tested the chamber at maximum flow rate.

Tested the chamber at our design flow rate.

Video and Still Images to follow.