Date post: | 31-Dec-2015 |
Category: |
Documents |
Upload: | drusilla-waters |
View: | 216 times |
Download: | 0 times |
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.
Jan-Willem de Gier
Center for Biomembrane Research, Stockholm, Sweden
Green Light for Membrane Proteins
MP-GFP overexpression
N
C
GFP
inclusion bodiesmembrane
N
CGFP
N
CGFP
N
CGFP
cytoplasmicmembrane
cytoplasm
periplasm
David Drew et al. Nat Methods (2006) 4:303-13
QuickTime™ and aTIFF (LZW) decompressor
are needed to see this picture.
Monitoring overexpression of a YedZ-GFP fusion using in-gel and whole-cell fluorescence
David Drew et al. Nat Methods (2006) 4:303-13
QuickTime™ and aTIFF (LZW) decompressor
are needed to see this picture.
From detergent screen to recovered membrane protein
David Drew et al. Nat Methods (2006) 4:303-13
C
N
MP-TEV-GFP-8His
GFP
TEV10His
8His
QuickTime™ and aTIFF (LZW) decompressor
are needed to see this picture.
Recovered membrane proteins can be usedfor functional and structural studies
David Drew et al. Protein Sci (2005) 14: 2011-2017
QuickTime™ and aTIFF (LZW) decompressor
are needed to see this picture.
0
0.5
1
1.5
2
2.5
3
Fur4 Azr1AGP1Isc1
Hsp30Sec61YOL162W
ITR1Shr3pHNM1HXT3HXT1UGA4MPH3ARN1DAL4TPO4DUR3KHA1Rft1SsH1 GAL
QuickTime™ and aTIFF (LZW) decompressor
are needed to see this picture.
Simon Newstead et al. PNAS (2007) 104(35):13936-41
GFP-based pipeline in yeast
Biogenesis of membrane proteins in E. coli
Samuel Wagner et al. (2006) Trends Biotechnol 24: 364-371
membrane proteins secretory proteins
QuickTime™ and aTIFF (LZW) decompressor
are needed to see this picture.
E. coli BL21(DE3)/pET overexpression system
Merck Novagen, pET manual
QuickTime™ and aTIFF (LZW) decompressor
are needed to see this picture.
Experimental setup (1)
Samuel Wagner et al. (2007) Mol Cell Proteomics (9) 1527-50.
QuickTime™ and aTIFF (LZW) decompressor
are needed to see this picture.
Experimental setup (2)
Samuel Wagner et al. (2007) Mol Cell Proteomics (9) 1527-50.
QuickTime™ and aTIFF (LZW) decompressor
are needed to see this picture.
Consequences of membrane protein overexpression in E. coli (1)
Samuel Wagner et al. (2007) Mol Cell Proteomics (9) 1527-50.
QuickTime™ and aTIFF (LZW) decompressor
are needed to see this picture.
Consequences of membrane protein overexpression in E. coli (2)
Samuel Wagner et al. (2007) Mol Cell Proteomics (9) 1527-50.
QuickTime™ and aTIFF (LZW) decompressor
are needed to see this picture.
Characterization of C41(DE3) and C43(DE3), also known as the Walker strains
QuickTime™ and aTIFF (LZW) decompressor
are needed to see this picture.
Differences in overexpression kinetics?
QuickTime™ and aTIFF (LZW) decompressor
are needed to see this picture.
Samuel Wagner et al. (2008) PNAS, 105 (38):14371-6.
Samuel Wagner et al. (2008) PNAS, 105 (38):14371-6.
QuickTime™ and aTIFF (LZW) decompressor
are needed to see this picture.
Differences in overexpression kinetics
Effect of the addition of IPTG
Samuel Wagner et al. (2008) PNAS, 105 (38):14371-6.
QuickTime™ and aTIFF (LZW) decompressor
are needed to see this picture.
Mutations in the promoter governing T7RNAP expression in C41(DE3) and C43(DE3)
Samuel Wagner et al. (2008) PNAS, 105 (38):14371-6.
QuickTime™ and aTIFF (LZW) decompressor
are needed to see this picture.
Mimicking the effect of the mutations in the promoters with T7 lysozyme
Samuel Wagner et al. (2008) PNAS, 105 (38):14371-6.
QuickTime™ and aTIFF (LZW) decompressor
are needed to see this picture.
Mimicking the effect of the mutations in the promoters with T7 lysozyme
Samuel Wagner et al. (2008) PNAS, 105 (38):14371-6.
QuickTime™ and aTIFF (LZW) decompressor
are needed to see this picture.
Characterization of Lemo21(DE3)
Samuel Wagner et al. (2008) PNAS, 105 (38):14371-6.
QuickTime™ and aTIFF (LZW) decompressor
are needed to see this picture.
Lemo21(DE3) is an ’all in one’ platform for membrane protein overexpression
Samuel Wagner et al. (2008) PNAS, 105 (38):14371-6.
QuickTime™ and aTIFF (LZW) decompressor
are needed to see this picture.
Funding-Swedish Research Council (VR)-Wallenberg Foundation-Swedish Foundation for Strategic Research (SSF)-National Institutes of Health (NIH)
QuickTime™ and aTIFF (LZW) decompressor
are needed to see this picture.
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.
www.xbranebio.com
www.cbr.su.se
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.