JOURNAL OF BACTERIOLOGYVOLUME 114 * NUMBER 3 * JUNE 1973

EDITORIAL BOARD

L. LEON CAMPBELL, Editor-in-Chiet'(1975)University of Delaware, Newark

R. L. ANDERSON, Editor (1975) ROY CURTISS III, Editor (1974)Michigan State UniversitY. East Lansing University ofAlabama, Birmingham

S. G. BRADLEY, Editor (1974) ALLEN G. MARR, Editor (1973)Virginia Commonwealth UniversitY, Richmond UniversitY of California. Davis

H. E. t'MBARGER, Editor (1976)Purdue University. Lafayette, Ind.

Martin Alexander (1975)W. Lane Barksdale (1975)M. E. Bayer (1975)Blaine Beaman (1976)Claire M. Berg (1973)Richard S. Berk (1974)Robert W. Bernlohr (1973)Herbert W. Boyer (1975)B. Wesley Catlin (1974)D. Jose%hiClark (1974)Stanley N. Cohen (1973)Ronald S. Cole (1974)S. F. Conti (1975)Nicholas R. Cozzarelli (1975)Walter J. Dobrogosz (1973)Martin Dworkin (1973)R. G. Eagon (1974)Alan D. Elbein (1973)Wolfgang Epstein (1974)Stanley Falkow (1975)Gerald R. Fink (1975)A. T. Ganesan (1975)Howard Goldfine (1975)Harry Gooder (1975)Joseph S. Gots(1975)Harlyn 0. Halvorson (1975)

ROBERT A. DAY,

F. M. Harold (1973)George Hegeman (1974)Donald R. Helinski (1975)Charles E. Helmstetter (1975)Robert K. Herman (1974)Joseph T. Holden (1975)Stanley C. Holt (1974)John Ingraham (1973)Sam Kaplan (1974)David E. Kennell (1974)Robert L. Lester (1975)Hillel S. Levinson (1975)E. C. C. Lin (1975)Michael H. Malamy (1975)Manley Mandel (1975)David Mardon (1974)Alvin Markovitz (1973)Neil H. Mendelson (1975)M. L. Morse (1975)Eugene W. Nester (1975)Richard P. Novick (1973)L. Nicholas Ornston (1975)Leo Parks(1973)Martin Pato (1975)Allen T. Phillips (1973)Lewis 1. Pizer (1975)

Managing Editor, 1913 1 St., N. W.,

Jack Preiss (1974)Charles C. Remsen III (1973)William S. Reznikoff (1973)H. V. Rickenberg (1973)Antonio H. Romano (1973)John Roth (1973)Harold L. Sadoff (1974)M. R. J. Salton (1975)David Schlessinger (1974)Carl Schnaitman (1974)Jane K. Setlow (1975)Helen Jean Shadomy (1975)Stanley K. Shapiro (1976)Simon Silver (1973)W. R. Sistrom (1975)Lucile Smith (1975)Paul S. Sypherd (1974)A. L. Taylor (1974)Donald J. Tipper (1975)Arthur Weissbach (1974)N. E. Welker (1973)Meyer J. Wolin (1973)Frank E. Young (1973)Stanley A. Zahler (1974)

Washington, D.C. 20006

EX OFFICIOR. G. E. MURRAY, President (1972- 1973)

DONALD E. SHAY, Secretary

The Journal of Bacteriology, a publication of the American Society forMicrobiology, 1913 St., N.W., Washington, D.C. 20006, is devoted tothe advancement and dissemination of fundamental knowledge concerningbacteria and other microorganisms. The Journal is published monthly, andthe twelve numbers are divided into four volumes per year. The subscrip-tion price is S75 (Postal Union Countries, S77: other foreign, $78) per year.Single copies are $7.00 (foreign, 7.25). Members of the American Societyfor Microbiology may receive the Journal as part of their dues. Corre-spondence relating to subscriptions, reprints, defective copies, availabilityof back issues, lost or late proofs, disposition of submitted manuscripts,and general editorial matters should be directed to the ASM PublicationsOffice. 1913 St., N.W., Washington, D.C. 20006 (area 202 833-9416).Correspondence from ASM members relating to membership dues,

L. LEON CAMPBELL, Vice-President (1972-1973)T. J. CARSKI, Treasurer

member subscriptions, changes of address, incorrect journals, etc., shouldbe directed to the Executive Secretary, American Society for Microbiol-ogy, 1913 St., N.W., Washington, D.C. 20006. Published monthly bythe ASM at 428 E. Preston St., Baltimore, Md. 21202.

Second class postage paid at Baltimore, Md. 21202, and at additionalmailing offices.Made in the United States of America.Copyright i 1973, American Society for Microbiology.All Rights Reserved.

Author Index

Allison, David P., 1328Anderson, W. Marshall, Jr., 988Antoniewski, J., 928Archibold, Errol R., 1007

Balkwill, D. L., 1319Ballou, Clinton E., 1058Bendler, John, 1151Birch, N., 1362Bose, R. J., 1365Braun volkmar, 1264Brill, *Vinston J., 1346Brown, Dennis T., 1351Bryant, M. P., 1231Buettner, M. J., 1068Burchard, Robert P., 1351

Capage, Domenick A., 1074Card, George L., 1125Casida, L. E., Jr., 1319Cherest, H., 928("lewell, D. B., 1362Colwell, Rita R., 897, 916Cooper, Stephen, 966Coote, J. G., 1254Corner, T. R., 1177Costerton, J. W., 1281Curtiss, Roy, III, 1328

Dann, L., 1225de Kloet, Siwo R., 1034Delwiche, E. A., 1206de Robichon-Szulmajster, H.,928

Dunlop, Winifred F., 1271Dunn, Etta, 943Dunn, N. W., 974Dunny, G. M., 1362

Eisenberg, A. D., 1177Epstein, Wolfgang, 1040

Foerster, Charlene Wehe, 1090Foerster, Harold F., 1090Forbes, E., 943Fridovich, Irwin, 1193

Gest, Howard, 1052, 1045Glauert, Audrey M., 1294Glickman Barry W., 951Gnirke, Iielga, 1264Goodgal, Sol, 1151Gregory, Eugene M., 1193

Gromkova, Rosa, 1151Gunsalus, I. C., 974Guterman, Sonia K., 1217, 1225Halvorson H. 0., 1158Harrison, T'homas L., 1074Hascall, G., 1362Hasegawa Takezi, 961Helinski, bonald R., 1116Henning, Ulf, 1264Henry, Susan A., 1158Hesse, Joanne E., 1040Higerd, Thomas B., 1184Hunter, Douglas R., 956

Iannotti, E. L., 1231Iijima, Teiji, 961Imai, Ko, 961Inderlied, C. B., 1206

Kafkewitz, D., 1231Karrer, E., 1365Keen, William A., 1074Kinghorn, J. R., 943Kingsbury, David T., 1116Kinross, J., 1014Krantz, Mark J., 1058Kuhrt, Michael, 1309

Leonard, C. Gomez, 1198Liddle, J., 1018MacGregor, C. H., 1164McKinney, R. M., 1018Mandelstam, J., 1254Marinus, M. G., 1143Marrs, Barry, 1052,1045Martini Elizabeth M., 1083Merlie, John P., 980Monk, M., 1014Morris, N. Ronald, 1143Moss, C. Wayne, 1018Munoz, Elaine F., 999

Neimark, Harold, 1025Norberg, Carol L., 956

Oliver, James D., 897Ou, Jonathan T., 1108Oujezdsky, Karen B., 1356

Panos, Charles, 934Pate, Jack L., 1309, 1346

Pateman, J. A., 943Perry, Anderson, 1328Piggot, P. J., 1241Pizer, Lewis I., 980Prasad, I., 909

Rehn, Kurt, 1264Remy, Charles N., 988Rickenberg, H. V., 1068Robards, A. W., 1271Rorsch, Arthur, 951Ross, Ian K., 1083Rothman-Denes, Lucia B., 1040Ruettinger, Therese, 966

Samuels, S. B., 1018Schaefler, S., 909Schnaitman, C. A., 1164Segel, Irwin H., 956Setlow, Peter 1099Shah, Vinod ik., 1346Sheehy, Ronald J., 1328Silverman, Melvin P., 999Simon, Robert D., 1213Sipe, Jerry E., 988Slabyj, Bohdan M., 934Sleytr, U. B., 1294Smith, Harriet, 1138Smith, Issar, 1138Spitz, Eva, 1068Spizizen, John, 1184Staley, T. E., 916Surdin-Kerjan, Y., 928Sutter, Richard P., 1074Szaniszlo, Paul J., 1356

Thoman, Marilyn, 1083Thornley, Margaret J., 1294Town, C., 1014Tung, Ming C., 1025

van der Maas, Gerda, 951van Sluis, Cornelis A., 951

Warren, R. A. J., 1365Welker, N. E., 1336Williams, L. S., 1007Wisdom, Charlene, 1336Woldringh, C. L. 1359Wolin, M. J., 1231

Young, B., 909

JOURNAL OF BACTERIOLOGYINSTRUCTIONS TO AUTHORS

Submit manuscripts in duplicate (original and onecarbon) to ASM Publications Office, 1913 I St., N.W.,Washington, D.C. 20006.

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Contributors should indicate, in the covering letteraccompanying their manuscript, which subject sec-tion of the Journal table of contents their papershould be listed under. However, final decision willbe made by the Editors.A charge of $25 per printed page is assessed for

publication in the Journal. Most institutions andgranting agencies in the United States permit thepayment of publication charges as a part of theirgeneral research support. It is recognized, however,that such funds are not always available to labora-tories outside the United States. In such cases, thecost of publication will be borne by the AmericanSociety for Microbiology.The "editorial style" of the Journal essentially fol-

lows the CBE Style Manual (3rd ed., AIBS, 1972).Genetics symbols should essentially follow the rec-ommendations of Demerec et al. (Genetics 54:61,1966). Biochemical abbreviations and nomenclatureshould essentially follow "Biochemical Nomencla-ture" in Handbook of Biochemistry (2nd ed., 1970,H. A. Sober, ed., The Chemical Rubber Co., Cleve-land, p. A4-A24). Normally, abbreviations (exceptthose of standard units of measurement and symbolsof the elements) should be defined and introducedparenthetically at first use in the text. Enzyme ac-tivity should be expressed in terms of internationalunits (Enzyme Nomenclature, Elsevier PublishingCo., 1965), and the EC number be given parentheti-cally at first use in the text. As a rule, all bacterialgrowth curves should be presented as semilogarith-mic plots (log x vs. time). Deviations from this policyare permitted only when justified in the text. In ex-pressing lengths, weights, and volumes, the prefixesnano (n) and pico (p) should be used instead of milli-micro (my) and micromicro (14,g). Express lengths innanometers (nm; 10-I m) or in micrometers (Mm; 10-1m) instead of millimicrons (mM; 10-' m), microns(M; 10- 6 m), or Angstroms (A; 10-10 m). Express partsper million (ppm) as micrograms per milliliter (Mg/ml),micrograms per gram (gg/g), or microliters per liter(Mliters/liter), as appropriate. In general, measure-ments should be expressed in terms of standard in-ternational metric units. The Journal reserves theprivilege of editing manuscripts to make them con-form with the adopted style.Form of manuscript. All parts of the manuscript

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Nomenclature of microorganisms. The name of aspecies is a binary combination consisting of thename of the genus followed by a specific epithet. Ingeneral, the nomenclature presented in Bergey'sManual of Determinative Bacteriology (7th ed., 1957)is used. If an author challenges this nomenclature,his own judgment will be followed, but the name inBergey's Manual should follow in parentheses thefirst time the name is used in the text and in the Ab-stract. When a new bacterial name is proposed in amanuscript, an international authority on nomencla-ture will be consulted for an opinion. When a newspecies, or a new variety of a species, is proposed, anacceptable photomicrograph or electron micrographof the cells should be submitted. If the cells aremotile, the photomicrograph or electron micrographshould show the nature and arrangement of flagella.As one of the requirements for description of a newspecies, we require deposition of the type culture ina recognized culture collection and designation of theaccession number.

Notes. The accepted form for Notes is somewhatdifferent from the foregoing. Contributors should con-sult a recent issue of the Journal for style. Notesshould not exceed 500 words. The Abstract shouldnot exceed 25 words.

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INDEX TO DATE OF ISSUE

DATE OF ISSUE

17 April 197317 May 197311 June 1973

MONTH

AprilMayJune

PAGES

1-462463-896897-1366

Adair, Frank W., 434Allam, A. M., 457Allison, David P., 439, 1328Anagnostopoulos, C., 18Anderson, W. Marshall, Jr., 988Andreesen, Jan R., 743Anker, Philippe, 114Antoniewski, J., 928Aoki, Hatsuo, 137Archibold, Errol R., 1007Arditti, Rita, 652Aten, Raymond F., 537Audit, C., 18Avni, Hanna, 891

Bak, A. Leth, 367Balkwill, D. L., 1319Ballesteros, Antonio O., 351Ballou, Clinton E., 1058Baptist, Jeremy E., 267Barash, H., 53Barton, Justice S., 782Bassel, John, 894Beckman, Bruce E., 790Beckman, Mary M., 798, 804Beckwith, Jon, 652Bendler, John, 1151Bhargava, Madhu M., 1Birch, N., 1362Bollon, Arthur P., 632Bose, R. J., 1365Botstein, David, 357Braun, Volkmar, 1264Brenchley, Jean E., 528, 666Brill, Winston J., 1346Brock, Thomas D., 706Brown, Ann, 743Brown, Dennis T., 1351Brown, Lyle R., 103Bryant, M. P., 1231Buchanan, Christine E., 891Buettner, M. J., 1068Burchard, Robert P., 1351

Calder, J. A., 695, 701Caldwell, Daniel R., 782Calmes, Robert, 249Capage, Domenick A., 1074Card, George L., 1125Carleton, Bruce, 612, 625Casida, L. E., Jr., 1319Chandler, Jerry LR, 128Cherest, H., 928Chin, Nena W., 674Christiansen, C., 367Christiansen, Gunna, 367Cirigliano, C., 273Clark, Alvin J., 424Claus, G. W., 378Clewell, D. B., 1362

AUTHOR INDEXVOLUME 114

Cole, Roger M., 860Colwell, Rita R., 897, 916Cooper, Stephen, 966Coote, J. G., 1254Conners, Judith H., 592Corner, T. R., 1177Cosloy, Sharon D., 679, 785Costerton, J, W., 1281Couch, John L., 228Coyette, Jacques, 34Coyne, Sheila I., 860Curtiss, Roy, III, 439, 1328

Dajani, Adnan S., 738Dann, L., 1225Davidoff-Abelson, R., 273Davies, Roland, 357Davis, Daniel B., 294Day, Richard A., 537Deal, S. J., 249de Kloet, Siwo R., 1034Delwiche, E. A., 1206DeMoss, R. D., 341de Robichon-Szulmajster, H.,928

Dooley, Douglas C., 711Dover, S., 53Duncan, Charles L., 845Dunlop, Winifred F., 1271Druck, Karnina, 53Dubnau, D., 273Dunn, Etta, 943Dunn, N. W., 974Dunny, G. M., 1362

Eagon, R. G., 399Edwards, David L., 164Ehrenspeck, Susan, 814Eisenberg, A. D., 1177Elzainy, T. A., 457Epstein, Wolfgang, 1040

Fan, David P., 790, 798, 804Fields, Marion L., 878Fisher, W. D., 874Floss, Heinz G., 208Foerster, Charlene Wehe, 1090Foerster, Harold F., 1090Forbes, E., 943Frank, Hilmer A., 878Fridovich, Irwin, 543, 1193Frieben, William R., 845Fried, Victor A., 239Friesen, Benjamin S., 267

Gale, Glen R., 460Garrick-Silversmith, Linda,507

Geftic, Sam G., 434

Gerhardt, Philipp, 888Gest, Howard, 1045, 1052Ghanekar, A. S., 618Ghei, Om. K., 65Gibson, F., 42Gibson, Jane, 86Gilleland, H. E., Jr., 399Gilvarg, Charles, 455Glauert, Audrey M., 1294Glickman, Barry W., 951Goldberger, Robert F., 351Goldman, Peter, 641Gnirke, Helga, 1264Goodgal, Sol, 1151Gorish, Helmut, 645Greenberg, B., 152Gregory, Eugene M., 543, 1193Grodzicker, Terri, 652Gromkova, Rosa, 1151Grossowicz, Nathan, 485Guest, J. R., 563Gunsalus, I. C., 974Guterman, Sonia K., 1217, 1225

Hageman, James H., 612Hall, Barry G., 448Hallock, Linda L., 860Halpern, Y. S., 53Halvorson, Harlyn O., 1, 1158Hanh, Vo Thi, 220Hanson, Ronald L., 772Harrison, Thomas L., 1074Harvey, R. J., 287, 309Hascall, G., 1362Hasegawa, Takezi, 961Hassan, M. M., 457Haworth, Stephen R., 103Helinski, Donald R., 577, 1116Helmstetter, Charles E., 294Hempfling, Walter P., 592Henneberry, R. C., 625Henning, Ulf, 1264Henry, Susan A., 1158Hershberger, C. L., 300Hesse, Joanne E., 1040Heymann, Hans, 434Hierholzer, John C., 257Higa, Edward H., 556Higerd, Thomas B., 1184Hoch, Sallie O'Neil, 341Hoffmann, Hans-Peter, 434Holmes, Walter M., 600Horstmann, Jonathan, 164Hua, Sui-Sheng, 891Hungate, R. E., 729Hunter, Douglas R., 956

Iannotti, E. L., 1231Iijima, Teiji, 961Imai, Ko, 961

AUTHOR INDEX

Inderlied, C. B., 1206Ingraham, J. L., 528Ingram, L. O., 695, 701, 874Istdriz, Tomas, 463, 469Ivarie, Robert D., 571

Jackson, Sally W., 838Jacobson, G. K., 876

Kafkewitz, D., 1231Kane, James F., 600Kaplan, J. Gordin, 323, 332Karrer, E., 1365Katz, Dvorah, 819Katz, Leonard, 577Kay, William W., 65Keen, William A., 1074Keeney, Mark, 782Kelley, Juanita F.: 782Kennedy, Eugene P., 772King, Gretchen J., 845Kinghorn, J. R., 943Kingsbury, David T., 577, 1116Kinross, J., 1014mloos, Wesley E., 169Kovach, John S., 351Krantz, Mark J., 1058Kuhrt, Michael, 1309Kuo, S.-C., 233Kwiecinski, Fabia, 164

Lacks, S., 152Lampen, J. O., 233Laurent, Simone J., 474Lawrence, Paul J., 220Leanz, Gary, 455Leonard, C. Gomez, 1198Leonard, Thomas J., 7Liddle, J., 1018Lin, E. C. C., 723, 767, 880Lingens, Franz, 645Ljungdahl, Lars G., 743Lugtenberg, E. J. J., 499

Macdonald, C. G., 42McDonald, Roderick A., 323,332

McFall, Elizabeth, 685McGinnis, James F., 885MacGregor, C. H., 1164Machtiger, Neal A., 80McKinney, R. M., 1018McMurrough, I., 451Mandelbaum-Shavit, Frede-

rika, 485Mandelstam, J., 1254Marinus, M. G., 1143Markovitz, Alvin, 891Marquis, Robert E., 491Marrs, Barry, 1045, 1052Martinez, Douglas, 882Martini, Elizabeth M., 1083Mateles, R. I., 390Matsuhashi, Michio, 453

Matsumura, Philip, 491Mendelson, Neil H., 860Merlie, John P., 980Meyers, Marilyn, 351Miki, K., 767Miller, M. W., 413Milstien, Sheldon, 641Miovic, Margaret L., 86Miranda, Mary, 413Miyai, Kenji, 723Monk, M., 1014Montenecourt, Bland S., 233Morris, C. F., 300Morris, N. Ronald, 1143Mortimer, Robert, 894Moss, C. Wayne, 1018Munoz, Elaine F., 999

Nagel de Zwaig, Rosa, 463, 469Nair, P. M., 618Nakayama, Hiroaki, 228Neimark, Harold, 1025Nester, Eugene W., 711Neurauter, Chris, 743Norberg, Carol L., 956Novick, Aaron, 239

Odds, Frank C., 257O'Leary, William M., 80Oliver, James D., 897Ou, Jonathan T., 1108Oujezdsky, Karen B., 1356

Paigen, Kenneth, 885Panos, Charles, 934Pardee, Arthur B., 603Parker, P. L., 695Parks, L. W., 876Pate, Jack L., 1309, 1346Patel, Nanu, 600Pateman, J. A., 943Pemberton, J. M., 424Pefne, Jacques J., 571Perry, Anderson, 1328Phillips, Lou Ellen, 7Piggot, P. J., 1241Pizer, Lewis I., 980Pledger, W. J., 183, 195Plucker, F. E., 695Porterfield, Nancy, 491Prasad, I., 909Pritchard, R. H., 824Proctor, Alan R., 169Pulich, Warren M., 28

Ramaley, Robert F., 556Reeve, John N., 860Reeves, Peter, 11Rehn, Kurt, 1264Remy, Charles N., 988Richey, D. P., 880Rickenberg, H. V., 1068Robards, A. W., 1271Robbers, James E., 208

Robertson, Larry W., 208Rogolsky, Marvin, 220Rorsch, Arthur, 951Rose, A. H., 451Rosenberger, R. F., 819Ross, Ian K., 1083Roth, Charles W., 592Rothman-Denes, Lucia B., 1040Rownd, R., 300Ruettinger, Therese, 966

Samuels, S. B., 1018Sanchez, Reina S., 463Satyanarayana, T., 332Schaefler, S., 909Schauder, Valerie R., 59Schaupp, Annabella, 743Scheie, Paul, 549, 814Scher, B., 273Scherrer, Rend, 888Schnaitman, C. A., 1164Scudiero, Dominic A., 267Sebastian, Jesus, 1Segel, Irwin H., 956Setlow, Peter, 1099Shah, Vinod K., 1346Sheehy, Ronald J., 439, 1328Shivvers, Douglas W., 706Shockman, Gerald D., 34Silverman, Melvin P., 999Simon, Robert D., 1213Sinskey, Anthony J., 357Sipe, Jerry E., 988Skurray, Ronald A., 11Slabyj, Bohdan M., 934Slayman, Clifford L., 752Slepecky, Ralph A., 137Sleytr, U. B., 1294Smith, Alayne B., 460Smith, Harriet, 1138Smith, Issar, 1138Smith, Kendric C., 121Smith, W. R., 729Solomon, Ellen, 723Soria, Marco, 351Spencer, Margaret E., 563Spitz, Eva, 1068Spizizen, John, 1184Staley, T. E., 916Stenderup, A., 367Sternlicht, E., 819Stieglitz, B., 390Stinnett, J. D., 399Stroobant, P., 42Stroun, Maurice, 114Sundar Raj, C. V., 656Surdin-Kerjan, Y., 928Sutter, Richard P., 1074Switzer, R. L., 517Szaniszlo, Paul J., 1356

Talens, L. T., 413Tamaki, Shigeo, 453Tanami, Yoh, 408

* @

J. BACTERIOL.

VOL. 114, 1973 AUTHOR INDEX

Tanooka, Hiroshi, 445Thauer, R. K., 443Thoman, Marilyn, 1083Thornley, Margaret J., 1294Torriani, Annamaria, 507Town, C., 1014Trela, John M., 674Tritz, Gerald J., 128Tung, Ming C., 1025

Umbarger, H. E., 183, 195

Van Baalen, Chase, 28, 695, 701van der Maas, Gerda, 951Vannier, Francoise S., 474van Sluis, Cornelis A., 951

van Zaane, D., 499Vishniac, Wolf V., 592Vogel, Henry J., 632Vold, Barbara S., 178

Waddington, Penelope B., 59Waindle, Louise M., 517Walmsley, Roger H., 144Wannamaker, Lewis, 738Ward, C. M., Jr., 378Warren, R. A. J., 1365Wecksler, Magda, 469Wehr, C. Timothy, 96Welker, N. E., 1336Whitehouse, Frank, Jr., 882Wijsman, H. J. W., 499

Williams, L. S., 1007Wisdom, Charlene, 1336Woldringh, C. L., 1359Wolin, M. J., 1231Woods, D. R., 59Wu, H. C., 656Wu, Po Chi, 603

Yamada, Yoshitsugu, 408Young, B., 909Young, I. G., 42Youngs, David A., 121Yu, Ida, 729

Zaritsky, A., 824Zey, Pinina N., 838Zwaig, Nod, 463, 469

iii

SUBJECT INDEX

VOLUME 114

Acetohydroxy-acid synthetaseBacillus sp., 674branched-chain amino acid synthesis, 332S. pombe, 332T. aquaticus, 674

Acetyl esterasesB. subtilis, 1184

Acholeplasma laidlawiilactate dehydrogenase, 1025

Acid inhibitionstreptococcal growth, 491

Acinetobacter sp.outer membranes, 1294

AcriflavineS. marcescens, 59

Acyl-coenzyme A synthetasecharacterization in N. asteroides, 249

Adenine nucleotideNeurospora, 752

Adenine nucleotide poolsChromatium growth, 86

Adenosine triphosphataseantibody inhibition, 772E. coli, 772

S-Adenosyl methioninemethionine biosynthetic enzymes, repression of,928

S. cerevisiae, 928Adenylate energy chargeChromatium growth, 86

Agmenellum quadruplicatumcroton oil and cell division, 874photoheterotrophic growth, 701

Alcaligenes faecalisfatty acids, 618

Alkaline phosphatasepseudomonad cell wall, 1281

Amidase mutantB. subtilis, 798

Ammonium regulationA. nidulans, 943

Anabaena cylindricacyanophycin granule, 1213

Anthranilate synthaseB. subtilis, 600complementation, intergeneric, 600P. aeruginosa, 600

Antimetabolites of D-alanyl-D-alaninebacterial insensitivity, 460

Arginine restrictionE. coli, 632

Arizona arizonaefatty acid composition, 80

Arthrobacter crystallopoieteswall ultrastructure and Gram characteristics,378

Aspartic transcarbamylaseB. subtilis sporulation, 517

Aspergillus nidulansammonium regulation, 943cycloheximide treatment of hyphae, 819subapical walls, 819

Aspergillus nigergluconate degradation (nonphosphorylated),457

AutocytotoxicityE. coli, 882

AutolysinL. acidophilus, 34

Autolytic N-acetylmuramidaseL. acidophilus, 34

Azotobacter vinelandiimembrane control, 1346

Bacillus alveitryptophanase, 341

Bacillus cereuspenicillin-binding component, 537poly-,B-hydroxybutyrate granules, 1271

Bacillus fastidiosusspore germination, 137

Bacillus megateriumcircular DNA, 625dipicolinic acid location in spores, 455glutamic acid decarboxylase in spores, 1090protoplasts and temperature, 1177spore germination, 1090

Bacillus stearothermophilusdipicolinate treatment, 878membranes, 1336phospholipid metabolism, 1125

Bacillus subtilisacetyl esterases, 1184amidase mutant, 798anthranilate synthase, 600aspartic transcarbamylase inactivation, 517benzylpenicillin (radioactive) binding, 220bryamycin resistance marker, 1138C4-dicarboxylic acid transport system, 65chromosome regions and cell membrane, 571chromosome replication initiation, 474clones diploid in the tryptophan region, 18DNA-membrane complexes, 711glycosidase, 804minicells, 860oxygen toxicity, 1193protease-deficient mutants, 612recombinatio\i-deficient mutants, 273RNA polymerase mutants, 103SPO2 attachment site, 1138spore germination, 507spore outgrowth, 507sporulation genes temporal order of expression,

1254sporulation in protease-deficient mutant, 612sporulation operons, 1241

iv

SUBJECT INDEX

superoxide dismutase, 1193transformation of germinated spores, 445tRNA changes as a function of development, 178walls, end and septal, 790

BacteriochlorophyllR. capsulata mutants, 1052synthesis regulation, 1052

Bacteriocin, staphylococcalinteraction with susceptible bacteria, 738

Bacteroides amylophilussodium and other growth requirements, 782

Benzylpenicillin (radioactive)binding to B. subtilis mutants, 220

Blakeslea trisporatrisporic acid identification, 1074

BlebsE. coli, 814

Blue-green algaeGPND, 28

Blue-green bacteriacell division stimulation, 874croton oil, 874photoheterotrophic growth, 695, 701

Bryamycin resistance markerB. subtilis, 1138

Bud formationM. krissii, 413

CalciumL-leucine transport, 956

Candida albicansglycoprotein acid phosphatase, 257

Candida sp., psychrophilictemperature variation on fatty acid composi-

tion, 451Carbohydrate metabolism

catabolite inhibition site, 885Cardiolipin

B. stearothermophilus, 1125Catabolite inhibitioncarbohydrate metabolism, 885

Cell envelopesproteins, extractable, 399

CellulolysisR. albus, 729

Cell wallsA. crystallopoietes, 378

Chlamydia psittaciminiature cell formation, 408

Chlorate resistanceE. coli mutants, 1164

Chorismate mutaseS. aureofaciens, 645

Chromatium sp.adenylate energy, 86growth, 86nucleotide pools, 86

Chromosome replication initiationB. subtilis, 474

Chromosome replication timeE. coli, 824

Circular deoxyribonucleic acidB. megaterium, 625

Circular R-factor deoxyribonucleic acidP. mirabilis, 300

cis/trans Testhistidine operon, 351

Citrobacter freundiifatty acid composition, 80

Claviceps sp.typtophan uptake, 208

Clostridium acidi-uriciCO2 reduction to formate, 443

Clostridium perfringensparacrystalline inclusion, 845sporulation, 845

Clostridium thermoaceticumenzymes, 743growth, 743

Cold sensitivityS. typhimurium hiRW mutant, 528

Colicin BE. coli, 1217enterochelin, 1217

Colicin El plasmidreplication, 1116

Colicins El and E2synthesis regulation, 577

Competencestreptococci, 1198

Complementation, intergenericanthranilate synthase subunits, 600

Coprinus sp.isozyme patterns, 1083mono- and dikaryons, 1083

Croton oilA. quadruplicatum, 874blue-green bacteria, 874cell division stimulation, 874

Cryptic plasmidsS. typhimurium, 439

Cyanophycin granuleA. cylindrica, 1213

Cyclic adenosine monophosphateE. coli, 1068plasmid DNA, 577

CycloheximideA. nidulans hyphae, 819L-leucine transport, 956

Cytophaga columnaristubules and plasma membranes, 1309

Deoxyribonucleic acidB. megaterium spore germination, 1099B. subtilis recombination-deficient mutants, 273E. coli treated with MMS, 267enterobacteria, 367extrachromosomal, 367methylase mutants, 1143MMS-induced breaks, 267S. cerevisiae nutritional shift-up, 876S. mutans plasmid, 1362S. typhimurium division, 966transcription, 114

Deoxyribonucleic acid-dependent ribonucleic acidpolymerase

S. cerevisiae, 1

VOL. 114, 1973 v

SUBJECT INDEX

Deoxyribonucleic acid-membrane complexB. subtilis, 711

Deoxyribonucleic acid methylase mutantsE. coli, 1143

Deoxyribonucleic acid polymerase IE. coli recA and recB derivatives, 1014plasmid replication, 1116

Deoxyribonucleic acid repairS. typhimurium, 357

Dicarboxylic acid transportB. szubtilis, 65

Dikaryon (Coprinus)isozyme pattern, 1083

DipicolinateB. stearothermophilus, 878

Dipicolinic acidB. megaterium spores, 455

Diplococcus pneumoniaecompetence and DNase, 152transformation, 152

Electron transport systemR. capsulata mutant, 1045

EnterobacteriaDNA (extrachromosomal), 367

Enterochelincolicin B, 1217E. coli, 1217, 1225

Escherichia coliadenosine triphosphatase, 772argE-argH expression, 632arginine restriction, 632autocytotoxicity, 882blebs, 814c-AMP, 1068c-AMP effects on plasmid DNA, 577cell division, 603chlorate-resistant mutants, 1164chromosome replication time, 824colicin B, 1217complementation between wild type and mutant

13-galactosidase, 448conjugation and lethal zygosis, 11DNA breaks, 267DNA methylase mutants, 1143DNA polymerase I, 1014enterochelin, 1217, 1225ethanol treatment, 239F'lac replication, 294fumarate reductase mutants, 563galactose operon, 891, 1040gluconate metabolism in mutants, 463, 469glutamate active transport, 53D-glutamic acid auxotroph, 499G3P-fumarate coupling, 769isoleucine metabolism, 183, 195isomeroreductase induction, 195lactose operon, 652lethal zygosis and conjugation, 11lipopolysaccharide deletion, 453mannitol membrane translocation, 723mating-pair formation, 144mating-pair inhibition, 1108methionyl-tRNA synthetase, 1007MMS treatment, 267

murein structural model, 1264NAD synthesis regulation, 128osmotic pressure, 549oxygen toxicity, 1193phenethyl alcohol treatment, 1359phospho-,6-glucosidase A, 909phospholipid synthesis, 980plasmid replication, 1116polAl and resAl, 951polAl exrA X-ray sensitivity, 121protein initiation, 309recA and recB derivatives, 1014regulatory mutant of gluconate, 469resAl and polAl mutations, 951ribosomes synthesizing protein, 287rRNA-adenine (N6-) methylase, 988D-serine transport, 679superoxide dismutase, 543, 1193T4e-sensitive mutants, 656thymineless death, 228toluene treatment, 1359trimethoprim, 309T4t-sensitive mutants, 656ubiquinone biosynthesis, 42valine metabolism, 183, 195

EthanolE. coli treated with, 239

Extractable proteinsEDTA and DMF, 399P. aeruginosa, 399

Fatty acidsactivation, 249A. faecalis, 618Candida sp., psychrophilic, 451N. asteroides, 249paracolons, 80P. maltophilia, 1018synthesis by A. faecalis, 618temperature variation, 451

Fermentation productsR. albus, 1231

F'lac replicationE. coli, 294

Folatecarrier-mediated transport, 485P. cerevisiae, 485

Formateformation from CO2 in C. acidi-urici, 443

fl Phagemating pair inhibition, 1108

Freeze-etchinggliding bacteria, 1351soil microflora, 1319

Frog auriclestranscription of bacterial DNA, 114

Fumarate reductaseE. coli mutants, 563

Fumarate reductionglycerol-3-phosphate dehydrogenation, 767

Galactose operonc-AMP, 1040E. coli, 891, 1040transcriptional control, 891

vi J. BACTERIOL.

SUBJECT INDEX

0-Galactosidasecomplementation between E. coli wild type andmutant, 448

Gas vesiclesH. halobium, 1058

Gliding bacteriasurface structure after freeze-etching, 1351

GluconateA. niger, 457degradation, nonphosphorylated, 457E. coli mutant, 463, 469

GluconeogenesisT. neapolitanus, 592

GlutamateE. coli mutant, 499

Glutamate synthesisK. aerogenes, 666

Glutamate transportE. coli, 53sodium and potassium requirements, 53

Glycerol-3-phosphate dehydrogenationfumarate reduction, 767

Glycerol phosphorylationS. aureus, 880

Glycoprotein acid phosphataseC. albicans, 257

GlycosidaseB. subtilis, 804

Guanidinosuccinic acid metabolismmicroflora, intestinal, 641

Haemophilus influenzaerestriction modification, 1151

Halobacterium halobiumgas vesicles, 1058

Hexose phosphate synthetasepseudomonad C, 390

Hexose repressionSaccharomyces mutants, 233

Histidine biosynthesiscis/trans test of first enzyme effect, 351S. typhimurium, 351

Histidine operonregulation and cis/trans test, 351

Hyphaecycloheximide treatment, 819

Inositol-less deathrespiratory mutant selection, 164

Intestinal microfloraguanidinosuccinic acid, 641

Isoleucine metabolismE. coli mutant, 183, 195

Isomeroreductase inductionE. coli mutant, 195genetics, 195

Klebsiella aerogenesglutamate synthesis, inhibition, 666

Lactate dehydrogenaseA. laidlawii, 1025

Lactobacillus acidophilusautolysin, 34autolytic N-acetylmuramidase, 34

Lactose operonE. coli mutants, 652

Lethal zygosisE. coli conjugation, 11

L-Leucine transportcalcium, 956cycloheximide, 956P. chrysogenum, 956

L-FormS. pyogenes, 934teichoic acid of, 934

Lipid synthesisS. cerevisiae sporulation, 1158

LipopolysaccharideE. coli sensitivity to antibiotics and lysozyme,453

T. pallidum, 838

Macromolecular synthesisB. subtilis spores, 507

Manganous ionsheat-shock requirement, 137

MannitolE. coli, 723membrane translocation, 723

Mating-pair formationcompetence, 144E. coli, 144

MembraneAcinetobacter, 934B. stearothermophilus, 1336

Membrane-binding sitesB. subtilis, 571

Membrane controlA. vinelandii, 1346

MesosomesP. aeruginosa, 434

Methanol metabolismpseudomonad C, 390

Methionine sulfoneglutamate synthesis, 666

Methionine sulfoximineglutamate synthesis, 666

Methionyl-transfer ribonucleic acid synthetaseE. coli, 1007S. typhimurium, 1007

Methyl methane sulfonateDNA breaks, 267E. coli treated with, 267

Metschnikowia krissiibud formation, 413

Micrococcus IysodeikticusB. subtilis glycosidase, 804walls as specific substrate, 804

MinicellsB. subtilis, 860

Mitochondrial respirationN. crassa mutants, 164

Monokaryon (Coprinus)isozyme pattern, 1083

MS2 phagemating pair inhibition, 1108

MureinE. coli, 1264structural model, 1264

*iiVOL. 114, 1973

SUBJECT INDEX

Naphthalene oxidationplasmid coding, 974P. putida, 974

Neurospora sp.adenine nucleotide levels, 752

Neurospora crassarespiratory mutants, 164

"Neutral" proteaseB. subtilis mutant, 612

Nicotinamide adenine dinucleotidesynthesis regulation, 128

Nitrate reductase activitychlorate-resistant mutants, 1164E. coli mutants, 1164

Nitrate reductionV. alcale8cens, 1206

Nocardia asteroidesfatty acid activation, 249

No8toc sp.photoheterotrophic growth, 695

Osmotic pressureE. coli, 549

Osmotic propertiesB. megaterium protoplasts, 1177

Outer membranesAcinetobacter, 1294

Oxygen inductionsuperoxide dismutase, 543

Oxygen toxicityE. coli and B. subtili8, 1193

Paracolonsfatty acid composition, 80

Paracrystalline inclusionC. perfringens sporulation, 845

Pediococcus cerevisiaefolate transport, 485

Penicillin-binding componentB. cereus, 537

Penicillium chyrsogenumL-leucine transport, 956

Phenethyl alcohol treatmentE. coli, 1359

Phenoloxidase activityS. commune reproductive cycle, 7

Phialophora dermatitidisconjugation, 1356

Phosphatidylethanolamine metabolismB. stearothermophilus, 1125Phosphatidylglycerol metabolismB. stearothermophilus, 1125

Phospho-,O-glucosidase Aconstitutive biosynthesis, 909E. coli, 909genetic determination, 909

Phospholipidgram-negative marine bacteria, 897

Phospholipid metabolismB. stearothermophilus, 1125

Photoheterotrophic growthA. guadruplicatum, 701Nostoc sp., 695

Plasma membranesC. columnaris, 1309

PlasmidP. aeruginosa, 424

Plasmid deoxyribonucleic acidc-AMP, 577S. mutans, 1362

Plasmid deoxyribonucleic acid-protein relaxationcomplex

c-AMP, 577Plasmid replicationE. coli, 1116

polAl and resAl mutationsE. coli K-12, 951

PolyaminesP. acidovorans, 1365

Poly-i-hydroxybutyrate granulesB. cereus, 1271

Polynucleotide sequenceV. parahaemolyticus strains, 916

Prodigiosin synthesisiron and salt concentration, 999S. marcescers, 999

ProteaseB. subtilis mutant, 612

Protein synthesisE. coli ribosomes, 287

Proteolytic enzymesB. subtilis sporulation, 1184

Proteus mirabiliscircular R-factor DNA, 300

Protoplaststemperature effects, 1177B. megaterium, 1177

Providencia alcalifaciensfatty acid composition, 80

Pseudomonad Chexose phosphate synthetase, 390methanol metabolism, 390

Pseudomonas sp.alkaline phosphatase, 1281wall-associated enzyme, 1281

Pseudomonas acidovoranspolyamines, 1365

Pseudomonas aeruginosaanthranilate synthase, 600mesosomes, 434plasmids, 424proteins, extractable, 399

Pseudomonas maltophiliabranched-chain hydroxy fatty acids, 1018

Pseudomonas putidanaphthalene oxidation, 974plasmid coding enzymes, 974

Pyridine nucleotide-dependent glucose dehydro-genase activity

blue-green algae, 28Pyridine nucleotide reduction

sulfide dependence, 592Thiobacillus neapolitanus, 592thiosulfate dependence, 592

Regulatory mutantE. coli gluconate system, 469gluconate system, 469

Replication initiationB. subtilis, 474

* *ii J. BACTERIOL.

SUBJECT INDEX

resAl and polAl mutationsE. coli K-12, 951

Respiratory electron transport systemR. capsulata, 1045

Respiratory mutantsN. crassa, 164

Restriction modificationH. influenzae, 1151

Reticulate body formationC. psittaci, 408

R-factor deoxyribonucleic acidP. mirabilis, 300S. typhimurium, 1328

Rhadopseudomonas capsulatabacteriochlorophyll synthesis, 1052respiratory electron transport system, 1045

Ribonuclease IS. typhimurium mutant, 96

Ribonucleic acidB. subtilis polymerase mutants, 103polymerase mutants, 103

Ribonucleic acid polymeraseyeast nuclei, 1

Ribosomal ribonucleic acid-adenine (N'-) meth-ylase

E. coli, 988Ribosomal ribonucleic acid cistronsyeast chromosomes, 1034

RibosomesE. coli, 287protein synthesis and growth rate, 287

Ruminococcus albuscellulolysis, 729glucose fermentation products, 1231

Saccharomyces carlsbergensisrRNA cistrons, 1034

Saccharomyces cerevisiaeS-adenosyl methionine-mediated repression, 928cell division after nutritional shift-up, 876DNA-dependent RNA polymerase, 1DNA synthesis after nutritional shift-up, 876methionine biosynthetic enzymes, 928nutritional shift-up, 876sporulation and lipid synthesis, 1158

Saccharomyces mutantshexose repression, 233

Saccharomycopsis lipolyticagenetic analysis, 894

Salmonella typhimuriumcryptic plasmids, 439division cycle DNA replication, 966DNA repair, 357histidine operon, 351hi8W mutant, 528methionyl-tRNA synthetase, 1007minicells containing R-factor DNA, 956RNase-deficient mutant, 96tricarboxylic acid transport, 961

Schizophyllum communephenoloxidase activity, 7reproductive cycle, 7

Schizosaccharomyce8 pombeacetohydroxy acid synthetase, 332

branched-chain amino acid synthesis, 323, 332threonine deaminase, 323

Septal wallsB. subtilis, 790

Serine hydroxamateE. coli phospholipid synthesis, 980phospholipid synthesis, 980

D-Serine transportE. coli, 679

Serratia marcescensacriflavine uptake and resistance, 59prodigiosin synthesis, 999

Soil microflorafreeze-etching, 1319

SPO2 attachment siteB. subtili8, 1138

Spore germinationB. fastidios8u, 137B. megaterium, 1090, 1099glutamic acid decarboxylase, 1090heat-shock requirement, 137

Sporulationlipid synthesis, 1158S. cerevisiae, 1158

Sporulation genesB. subtilis, 1254temporal order of expression, 1254

Sporulation operonsB. subtilis, 1241

Staphylococcus aureusglycerol phosphorylation, 880tryptophan biosynthetic enzymes, 169

Streptococcus sp.competence, 1198intracellular ions, 491titration, 491

Streptococcus faecalissuperoxide dismutase induction, 543

Streptococcus mutansplasmid DNA, 1362

Streptococcus pyogenesL-form, 934teichoic acid of L-form, 934

Streptomyces aureofacienschorismate mutase, 645

Sulfolobus acidocaldariussulfur oxidation, 706

Sulfur oxidationS. acidocaldariub, 706

Superoxide dismutaseE. coli, 543E. coli and B. subtilis, 1193induction by 02, 543S. faecalis, 543

Teichoic acidS. pyogenes L-form, 934

Tetrazolium dyerespiratory mutant selection, 164

Thermus sp.threonine deaminase, 556

Thermus aquaticusacetohydroxy-acid synthetase, 674

Thiobacillus neapolitanus

VOL. 114, 1973 iX

SUBJECT INDEX

gluconeogenesis, 592pyridine nucleotide reduction, 592

Threonine deaminasebranched-chain amino acid synthesis, 323purification and properties, 556S. pombe, 323Thermus sp. 556

Thymineless deathassay systems, 228E. coli, 228

Toluene treatmentE. coli, 1359

Transcriptionbacterial DNA in frog auricles, 114

Transfer ribonucleic acidB. subtilis, 178changes as a function of development, 178

TransformationB. subtilis germinated spores, 445competence and DNase, 152D. pneumoniae, 152

Treponema pallidumnlipopolysaccharide, 838

Tricarboxylic acidS. typhimurium, 961

Tricarboxylic acid cycleC4-dicarboxylic acid transport, 65

TrimethoprimB. coli, 309protein initiation, 309

Trisporic acidBlakeslea trispora, 1074mating-type-specific components, 1074Mucor assay, 1074

TryptophanaseB. alvei, 341catalytic studies, 341

Tryptophan enzymesS. aureus, 169

Tryptophan genome regionB. subtilis, 18

Tryptophan uptakeClaviceps sp., 208

TubulesC. columnaris, 1309

Ubiquinone biosynthesisE. coli, 42gene-enzyme relationships, 42intermediates, 42

Valine metabolismE. coli, 183, 195

Veillonella alcalescensnitrate reduction and growth, 1206

Vibrio parahaemolyticuspolynucleotide sequence, 916

Zygosis (lethal)E. coli conjugation, 11

x J. BACTERIOL.

JOURNAL

BACTERIOLOGY

VOLUME 114

BALTIMORE, MD. 21202

1973

JOURNAL OF BACTERIOLOGYVOLUME 114

EDITORIAL BOARD

L. LEON CAMPBELL, Editor-in-Chiet'(1975)University of Delaware, Newark

R. L. ANDERSON, Editor (1975) ROY CURTISS 111, Editor (1974)Michigan State University, East Lansing University ofAlabama, Birmingham

S. G. BRADLEY, Editor (1974) ALLEN G. MARR, Editor (1973)Virginia Commonwealth University, Richmond University of California, Davis

H. E. t'MBARGER, Editor (1976)Purdue University, Lafayette, Ind.

Martin Alexander (1975)W. Lane Barksdale (1975)M. E. Bayer(1975)Blaine Beaman (1976)Claire M. Berg (1973)Richard S. Berk (1974)Robert W. Bernlohr (1973)Herbert W. Boyer (1975)B. Wesey Cadin (1974)D. Joseph Clark (1974)Stanley N. Cohen (1973)Ronald S. Cole (1974)S. F. Conti (1975)Nicholas R. Cozzarelli (1975)Walter J. Dobrogosz (1973)Martin Dworkin (1973)R. G. Eagon (1974)Alan D. Elbein (1973)Wolfgang Epstein (1974)Stanley Falkow (1975)Gerald R. Fink (1975)A. T. Ganesan (1975)Howard Goldfine (1975)Harry Gooder (1975)Joseph S. Gots(1975)Harlyn 0. Halvosn (1975)

F. M. Harold (1973)George Hegeman (1974)Donald R. Helinski (1975)Charles E. Helmstetter (1975)Robert K. Herman (1974)Joseph T. Holden (1975)Stanley C. Holt (1974)John Ingraham (1973)Sam Kaplan (1974)David E. Kennell (1974)Robert L. Lester (1975)Hillel S. Levinson (1975)E. C. C. Lin (1975)Michael H. Malamy (1975)Manley Mandel (1975)David Mardon (1974)Alvin Markovitz (1973)Neil H. Mendelson (1975)M. L. Morse (1975)Eugene W. Nester (1975)Richard P. Novick (1973)L. Nicholas Ornston (1975)Leo Parks (1973)Martin Pato (1975)Allen T. Phillips (1973)Lewis 1. Pizer (1975)

Jack Preiss (1974)Charles C. Remsen III (1973)William S. Reznikoff (1973)H. V. Rickenberg (1973)Antonio H. Romano (1973)John Roth (1973)Harold L. Sadoff (1974)M. R. J. Salton (1975)David Schlessinger (1974)Carl Schnaitman (1974)Jane K. Sedow (1975)Helen Jean Sbadomy (1975)Stanley K. Shapiro (1976)Simon Silver (1973)W. R. Sistrom (1975)Lucile Smith (1975)Paul S. Sypherd (1974)A. L. Taylor (1974)Donald J. Tipper (1975)Arthur Weissbach (1974)N. E. Welker (1973)Meyer J. Wolin (1973)Frank E. Young (1973)Stanley A. Zahler (1974)

ROBERT A. DAY, Managing Editor, 1913 1 St., N. W., Washington, D.C. 20006

EX OFFICIOR. G. E MURRAY, President (1972- 1973)

DONALD E. SHAY, Secretary

The Journl of Bactenology, a publication ofthe American Society forMicrobiology, 1913 I St., N.W., Washington, D.C. 20006, is devoted tothe advancement and dissemination of fundamental knowledge concerningbacteria and other microorganisms. The Journal is published monthly, andthe twelve numbers are divided into four volumes per year. The subscrip-tion price is $75 (Postal Union Countries, $77: other foreign, S78) per year.Single copies are $7.00 (foreign, 7.25). Members of the American Societyfor Microbiology may receive the Journal as pars of their dues. Corre-spondence relating to subscriptions, reprints, defective copies, availabilityof back issues, lost or late proofs, disposition of submitted manuscripts,and general editorial matters should be directed to the ASM PublicationsOffice. 1913 1 St., N.W., Washington, D.C. 20006 (area 202 833-9416).Correspondence from ASM members relating to membership dues,

L. LEON CAMPBELL, Vice-President (1972-1973)T. J. CARSKI, Treasurer

member subscriptions, changes of address, incorrect journals, etc., shouldbe directed to the Executive Secretary, American Society for Microbiol-ogy, 1913 1 St., N.W., Washington, D.C. 20006. Published monthly bythe ASM at 428 E. Preston St., Baltimore, Md. 21202.

Second class postage paid at Baltimore, Md. 21202, and at additionalmailing offices.Made in the United States of America.Copyright 0) 1973, American Society for Microbiology.All Rights Reserved.

Volume 114 Contents for April NumberI

Morphology and UltrastructureGram Characteristics and Wall Ultrastructure of Arthrobacter crystallopoietes During

Coccus-Rod Morphogenesis. C. M. WARD, JR., AND G. W. CLAUS............. 378Proteins Released from Cell Envelopes of Pseudomonas aeruginosa on Exposure to

Ethylenediaminetetraacetate: Comparison with Dimethylformamide-ExtractableProteins. J. D. STINNETT, H. E. GILLELAND, JR., AND R. G. EAGON............ 399

Mliniature Cell Formation in Chlamydia psittaci. YOH TANAMI AND YOSHITSUGUYAMADA ............................................................... 408

Electron Micrography of Bud Formation in Metschnikowia krissii. L. T. TALENS,MARY MIRANDA, AND M. W. MILLER...................................... 413

Mesosomes in Pseudomonas aeruginosa. HANS-PETER HOFFMANN, SAM G. GEFATIC,HANS HEYMANN, AND FRANK W. ADAIR.................................... 434

Dipicolinic Acid Location in Intact Spores of Bacillus megaterium. GARY LEANZ ANDCHARLES GILVARG...................................................... 455

Genetics and Molecular BiologyPhysiology of Escherichia coli K-12 During Conjugation: Altered Recipient Cell

Functions Associated with Lethal Zygosis. RONALD A. SKURRAY AND PETERREEVES ................................................................ 11

Genetic Studies Relating to the Production of Transformed Clones Diploid in theTryptophan Region of the Bacillus subtilis Genome. C. AUDIT AND C. ANAG-NOSTOPOULOS........................................................... 18

Isolation and Properties of a Ribonuclease-Deficient Mutant of Salmonella typhi-murium. C. TIMOTHY WEHR.............................................. 96

Genetic Analysis of Ribonucleic Acid Polymerase Mutants of Bacillus subtilis.STEPHEN R. HAWORTH AND LYLE R. BROWN............................... 103

Transcription of Spontaneously Released Bacterial Deoxyribonucleic Acid in FrogAuricles. MAURICE STROUN AND PHILIPPE ANKER........................... 114

X-Ray Sensitivity and Repair Capacity of a polAl exrA Strain of Escherichia coliK-12. DAVID A. YOUNGS AND KENDRIC C. SMITH............................ 121

Physical Assay of Competence for Specific Mating-Pair Formation in Escherichia coli.ROGER H. WALMSLEY.................................................... 144

Competence for Deoxyribonucleic Acid Uptake and Deoxyribonuclease Action Ex-ternal to Cells in the Genetic Transformation of Diplococcus pneumoniae. S.LACKS AND B. GREENBERG............................................... 152

Analysis of Isoaccepting Transfer Ribonucleic Acid Species of Bacillus subtilis: Changesin Chromatography of Transfer Ribonucleic Acids Associated with Stage of De-velopment. BARBARA S. VOLD............................................ 178

Isoleucine and Valine Metabolism in Escherichia coli. XXI. Mutations Affecting Dere-pression and Valine Resistance. W. J. PLEDGER AND H. E. UMBARGER........... 183

Isoleucine and Valine Metabolism in Escherichia coli. XXII. A Pleiotropic MutationAffecting Induction of Isomeroreductase Activity. W. J. PLEDGER AND H. E.UMBARGER............................................................. 195

Thymineless Death in Escherichia coli in Various Assay Systems: Viability Deter-mined in Liquid Medium. HIROAKI NAKAYAMA AND JOHN L. COUCH........... 228

Organic Solvents as Probes for the Structure and Function of the Bacterial Mem-brane: Effects of Ethanol on the Wild Type and an Ethanol-Resistant Mutant ofEscherichia coli K-12.VICTOR A. FRIED AND AARON NOVICK................... 239

Physiological Modifications in the Production and Repair of Methyl Methane Sul-fonate-Induced Breaks in the Deoxyribonucleic Acid of Escherichia coli K-12.DOMINIC A. SCUDIERO, BENJAMIN S. FRIESEN, AND JEREMY E. BAPTIST.......... 267

Fate of Transforming Deoxyribonucleic Acid After Uptake by Competent Bacillussubtilis: Phenotypic Characterization of Radiation-Sensitive Recombination-Deficient Mutants. D. DUBNAU, R. DAVIDOFF-ABELSON, B. SCHER, AND C.CIRIGLIANO............................................................. 273

Fraction of Ribosomes Synthesizing Protein as a Function of Specific Growth Rate.R. J. HARVEY.......................................................... 287

xiii

Control of F'lac Replication in Escherichia coli B/r. DANIEL B. DAVIS AND CHARLESE. HELMSTETTER........................................................ 294

Strand-Specific Nick in Open Circular R-Factor Deoxyribonucleic Acid: Attachmentof the Linear Strand to a Proteinaceous Cellular Component. C. F. MORRIS, C. L.HERSHBERGER, AND R. ROWND........................................... 300

Growth and Initiation of Protein Synthesis in Escherichia coli in the Presence of Tri-methoprim. R. J. HARVEY ........................... ......... 309

A cis/trans Test of the Effect of the First Enzyme for Histidine Biosynthesis onRegulation of the Histidine Operon. JOHN S. KOVACH, ANTONIO O. BALLESTEROS,MARILYN MEYERS, MARCO SORIA, AND ROBERT F. GOLDBERGER............... 351

Deoxyribonucleic Acid Repair in a Highly Radiation-Resistant Strain of Salmonellatyphimurium. ROLAND DAVIES, ANTHONY J. SINSKEY, AND DAVID BOTSTEIN. ...357

Extrachromosomal Deoxyribonucleic Acid in Different Enterobacteria. C. CHRISTIAN-SEN, GUNNA CHRISTIANSEN, A. LETH BAK, AND A. STENDERUP................. 367

Detection and Characterization of Plasmids in Pseudomonas aeruginosa Strain PAO.J. MA.PEMBERTON AND ALVIN J. CLARK.................................... 424

Cryptic Plasmids in a Minicell-Producing Strain of Salmonella typhimurium. RONALDJ. SHEEHY, DAVID P. ALLISON, AND ROY CURTISS III........................ 439

Transformation of Germinated Spores of Bacillus subtilis on Agar Plates. HIROSHITANOOKA ............................................................. 445

In Vivo Complementation Between Wild-Type and Mutant ,B-Galactosidase inEscherichia coli. BARRY G. HALL .......................................... 448

Physiology and MetabolismStudy of Phenoloxidase Activity During the Reproductive Cycle in Schizophyllum

commune. THOMAS J. LEONARD AND Lou ELLEN PHILLIPS.................... 7Pyridine Nucleotide-Dependent Glucose Dehydrogenase Activity in Blue-Green

Algae. WARREN M. PULICH AND CHASE VAN BAALEN........................ 28Pathway for Ubiquinone Biosynthesis in Escherichia coli K-12: Gene-Enzyme Rela-

tionships and Intermediates. I. G. YOUNG, P. STROOBANT, C. G. MACDONALD,AND F. GIBSON................. 42

Sodium and Potassium Requirements for Active Transport of Glutamate by Eschc-erichia coli K-12. Y. S. HALPERN, H. BARASH, S. DOVER, AND KARNINA DRUCK.. 53

Acriflavine Uptake and Resistance in Serratia marcescens Cells and Spheroplasts.D. R. WOODS, VALERIE R. SCHAUDER, AND PENELOPE B. WADDINGTON........ 59

Properties of an Inducible C4-Dicarboxylic Acid Transport System in Bacillus sub-tilis. OM. K. GHEI AND WILLIAM W. KAY.................................. 65

Fatty Acid Compositions of Paracolons: Arizona, Citrobacter, and Providencia.NEAL A. MACHTIGER AND WILLIAM Al. O'LEARY...................... 80

Nucleotide Pools and Adenylate Energy Charge in Balanced and Unbalanced Growthof Chromatium. MARGARET L. AMIoVIC AND JANE GIBSON.................... 86

Recognition of a Gene Involved in the Regulation of Nicotinamide Adenine Dinu-cleotide Biosynthesis. GERALD J. TRITZ AND JERRY LR CHANDLER............ 128

Inducement of a Heat-Shock Requirement for Germination and Production of In-creased Heat Resistance in Bacillus fastidiosus Spores by Manganous Ions.HATSUO AOKI AND RALPH A. SLEPECKY.................................... 137

Selection of Respiratory Mutants of Neurospora crassa. DAVID L. EDWARDS, FABIAKWIECINSKI, AND JONATHAN HORSTMANN.................................. 164

Tryptophan Biosynthetic Enzymes of Staphylococcus aureus. ALAN R. PROCTOR ANDWESLEY E.KLOOS.169

Some Characteristics of Tryptophan Uptake in Claviceps Species. LARRY W. ROBERT-SON, JAmEs E. ROBBERS,AND HEINZ G. FLOSS . ...................... 208

Binding of Radioactive Benzylpenicillin to Asporogenous Mutants of Bacillus sub-tilis During Postexponential Growth. MARVIN ROGOLSKY, PAUL J. LAWRENCE,AND Vo THI HANH .................................. ............220Saccharomyces Mutants with Invertase Formation Resistant to Repression by Hex-oses. BLAND S. MONTENECOURT, S.-C. KUO, AND J. 0. LAMPEN............... 233

Methanol Metabolism in Pseudomonad C. B. STIEGLITZ AND R. I. MATELES..... 390

xiv CONTENTS

CO2 Reduction to Formate in Clostridium acidi-urici. R. K. THAUER............. 443Effects of Temperature Variation on the Fatty Acid Composition of a Psychro-

philic Candida Species. I. MCM\'IURROUGH AND A. H. ROSE.................. 451Increase in Sensitivity to Antidiotics and Lysozyme on Deletion of Lipopolysaccha-

rides in Escherichia coli Strains. SHIGEo TAMAKI AND M\1ICHIO MATSUHASHI . 453Insensitivity of Bacteria to Proposed Antimetabolites of D-Alanyl-D-Alanin.c GLEN

R. GALE AND ALAYNE B. SMITH....................... ........ ... 460

EnzymologyNuclear Deoxyriboinucleic Acid-Dependent Ribonucleic Acid Polymerases from

Saccharomyces cerevisiae. JEsus SEBASTIAN, MADHU M. BHARGAVA, AND HARLYN0. HALVORSON.......................................................... 1

Some Properties of the Autolytic N-Acetylmuramidase of Lactobacillus acidophilus.JACQUES COYETTE AND GERALD D. SHOCKMAN.............................. 34

Fatty Acid Activation by a Lipophilic Bacterium. ROBERT CALMES AND S. J. DEAL 249Purification and Properties of a Glycoprotein Acid Phosphatase from Candida albi-

cans. FRANK C. ODDS AND JOHN C. HIERHOLZER............................ 257Biosynthesis of Branched-Chain Amino Acids in Schizosaccharomyces pombe: Regu-

latory Properties of Threonine Deaminase. RODERICK A. MCDONALD AND J.GORDIN KAPLAN.........................................................323Biosynthesis of Branched-Chain Amino Acids in Schizosaccharomyces pombe: Proper-ties of Acetohydroxy Acid Synthetase. RODERICK A. McDONALD, T. SATYA-NARAYANA, AND J. G. KAPLAN............................................ 332

Catalytic Studies on Tryptophanase from Bacillus alvei. SALLIE O'NEIL HOCH ANDR. D. DEMoss......................................................... 341

New Pathway for Nonphosphorylated Degradation of Gluconate by Aspergillusniger. T. A. ELZAINY, M. M. HASSAN, AND A. M. ALLAM..................... 457

Volume 114 Contents for May Number 2

Morphology and UltrastructureStructural Difference Between Walls from Hemispherical Caps and Partial Septa of

Bacillus subtilis. DAVID P. FAN AND BRUCE E. BECKMAN..................... 790Large Surface Blebs on Escherichia coli Heated to Inactivating Temperatures. PAUL

SCHEIE AND SUSAN EHRENSPECK......................................... 814Subapical Wall Synthesis and Wall Thickening Induced by Cycloheximide in Hyphae

of Aspergillus nidulans. E. STERNL1CHT, DVORAH KATZ, AND R. F. ROSENBERGER. 819Ultrastructure of Lipopolysaccharide Isolated from Treponema pallidum. SALLY W.

JACKSON AND PININA N. ZEY............................................ 838

Genetics and Molecular BiologyTemperature-Sensitive Initiation of Chromosome Replication in a 'iMutant of Bacillus

subtilis. SIMONE J. LAURENT AND FRANCOISE S. VANNIER.................... 474Isolation and Properties of Fumarate Reductase Mutants of Escherichia coli.

MARGARET E. SPENCER AND J. R. GUEST................................ 563Association of Many Regions of the Bacillus subtilis Chromosome with the Cell Mem-

brane. ROBERT D. IVARIE AND JACQUES J. PE'NE............................ 571Stimulation by Cyclic Adenosine AMonophosphate of Plasmid Deoxyribonucleic Acid

Replication and Catabolite Repression of the Plasmid Deoxyribonucleic Acid-Protein Relaxation Complex. LEONARD KATZ, DAVID T. KINGSBURY, ANDDONALD R. HELINSKI................................................... 577

Intergeneric Complementation of Anthranilate Synthase Subunits. NANU PATEL,WALTER A. HOLMES, AND JAMES F. KANE................................. 600

Characterization of Polydisperse Closed Circular Deoxyribonucleic Acid MIoleculesof Bacillus megaterium. R. C. HENNEBERRY AND BRUCE C. CARLTON.......... 625

CONTENTS xv

Regulation of argE-argH Expression with Arginine Derivatives in Escherichia coli:Extreme Non-Uniformity of Repression and Conditional Repressive Action.ARTHUR P. BOLLON AND HENRY J. VOGEL................................ 632

Cyclic Adenosine Monophosphate-Independent Mutants of the Lactose Operon ofEscherichia coli. RITA ARDITTI, TERRI GRODZICKER, AND JON BECKWITH....... 652

Escherichia coli Mutants Permissive for T4 Bacteriophage with Deletion in e Gene(Phage Lysozyme). C. V. SUNDAR RAJ AND H. C. Wu.................... . 656

D-Serine Transport System in Escherichia coli K-12. SHARON D. COSLOY ........ 679Deoxyribonucleic Acid-Membrane Complexes in the Bacillus subtilis Transformation

System. DOUGLAS C. DOOLEY AND EUGENE W. NESTER..................... 711Minicells of Bacillus subtilis. JoHN N. REEVE, NEIL H. MENDELSON, SHEILA I.

COYNE, LINDA L. HALLOCK, AND ROGER M. COLE .......................... 860Cell Division and Deoxyribonucleic Acid Synthesis After a Nutritional Shift-Up of

Saccharomyces cerevi8iae.. G. K. JACOBSON AND L. W. PARKS ..876Selective Autocytotoxicity in a Model System of Escherichia coli K-12 Recombinants.

DOUGLAS MARTINEZ AND FRANK WHITEHOUSE, JR......................... 882Transcriptional Control of the Galactose Operon by the capR (lon) and capT Genes.

CHRISTINE E. BUCHANAN, SUI-SHENG HUA, HANNA AVNI, AND ALVINMARKOVITZ...... ; 891

Genetic Analysis of Mating Type and Alkane Utilization in Saccharomycopsis lipo-lytica. JOHN BASSEL AND ROBERT MORTimER.894

Physiology and MetabolismMutations Affecting Gluconate Metabolism in Escherichia coli. RosA NAGEL

DE ZWAIG, Not ZWAIG, TOMAS ISTtIRIZ, AND REINA S. SANCHEZ.463Regulatory Mutations Affecting the Gluconate System in Escherichia coli. NoE

ZWAIG, ROSA NAGEL DE ZWAIG, TOMxS ISTTRIZ, AND MAGDA WECKSLER. 469Carrier-Mediated Transport of Folate in a Mutant of Pediococcus cerevisiae. FREDERIKA

MANDELBAUM-SHAVIT AND NATHAN GROSSOWCZ.485Acid-Base Titration of Streptococci and the Physical States of Intracellular Ions.

ROBERT E. MARQUIS, NANCY PORTERFIELD, AND PHILIP MATSUMURA........ 491Properties of a D-Glutamic Acid-Requiring Mutant of Escherichia coli. E. J. J. LUGTEN-

BERG, H. J. W. WIJSMAN, AND D. VAN ZAANE .............................. 499Macromolecular Syntheses During Germination and Outgrowth of Bacillus subtilis

Spores. LINDA GARRICK-SILVERSMITH AND ANNAMARIA TORRIANI.............. 507Inactivation of Aspartic Transcarbamylase in Sporulating Bacillus subtilis: Demon-

stration of a Requirement for Metabolic Energy. LOUISE MV. WAINDLE ANDR. L. SWITZER.......................................................... 517

Characterization of a Cold-Sensitive hisW Mutant of Salmonella typhimurium. JEANE. BRENCHLEY AND J. L. INGRAHAM....................................... 528

Osmotic Pressure in Escherichia coli as Rendered Detectable by Lysozyme Attack.PAUL SCHEIE........................................................... 549

Thiosulfate- and Sulfide-Dependent Pyridine Nucleotide Reduction and Gluconeo-genesis in Intact Thiobacillus neapolitanus. CHARLES W. ROTH, WALTER P.HEMPFLING, JUDITH N. CONNERS, AND WOLF V. VISHNIAC................... 592

Cell Division of Escherichia coli: Control by Membrane Organization. Po C. WUAND ARTHUR B. PARDEE .................................... 603

Effects of Mutational Loss of Specific Intracellular Proteases on the Sporulation ofBacillus subtilis. JAMES H. HAGEMAN AND BRUCE C. CARLTON................ 612

Evidence for the Existence of an Aerobic Pathway for Synthesis of MonounsaturatedFatty Acids by Alcaligenesfaecalis. A. S. GHANEKAR AND P. M. NAM.618

Role of Intestinal Microflora in the Metabolism of Guanidinosucciic Acid. SHELDONMILSTIEN AND PETER GOLDMAN ........................................... 641

Effect of Methionine Sulfoximine and Methionine Sulfone on Glutamate Synthesis inKlebsiella aerogenes. JEAN E. BRENCHLEY................................... 666

Metabolism of D-Serine in Escherichia coli K-12: Mechanism of Growth Inhibition.SHARON D. COSLOY AND ELIZABETH MCFALL.685

XVi CONTENTS

Role of Reduced Exogenous Organic Compounds in the Physiology of the Blue-GreenBacteria (Algae): Photoheterotrophic Growth of a "Heterotrophic" Blue-GreenBacterium. L. 0. INGRAM, J. A. CALDER, C. VAN BAALEN, F. E. PLUCKER, ANDP. L. PARKER...................................... ................... 695

Role of Reduced Exogenous Organic Compounds in the Physiology of the Blue-GreenBacteria (Algae): Photoheterotrophic Growth of an "Autotrophic" Blue-GreenBacterium. L. 0. INGRAM, C. VAN BAALEN, AND J. A. CALDER................ 701

Oxidation of Elemental Sulfur by Sulfolobus acidocaldarius. DOUGLAS W. SHIVVERSAND THOMAS D. BROCK.................................................. 706

Factors Affecting Cellulolysis by Ruminococcus albus. W. R. SMITH, IDA Yu, AND R. E.HUNGATE.............................................................. 729

Kinetic Studies on the Interaction of Bacteriophage Type 71 Staphylococcal Bac-teriocin with Susceptible Bacteria. ADNAN S. DAJANI AND LEWIs W. WANNAMAKER................................ 738

Fermentation of Glucose, Fructose, and Xylose by Cilostridium thermoaceticum: Effectof Metals on Growth Yield, Enzymes, and the Synthesis of Acetate from CO2. JANR. ANDREESEN, ANNABELLA SCHAUPP, CHRIs NEuRAuTER, ANN BROWN, ANDLARS G. LJUNGDAHL..................................................... 743

Adenine Nucleotide Levels in Neurospora, as Influenced by Conditions of Growth andby Metabolic Inhibitors. CLIFFORD L. SLAYMAN............................. 752

Sodium and Other Inorganic Growth Requirements of Bacteroides amylophilus. DANIELR. CALDWELL, MARK KEENEY, JUSTICE S. BARTON, AND JUANITA F. KELLEY. 782

Mutant of Bacillus subtilis with a Temperature-Sensitive Autolytic Amidase. DAVIDP. FAN AND MARY M. BECKMAN.......................................... 798

Micrococcus lysodeikticus Bacterial Walls as a Substrate Specific for the AutolyticGlycosidase of Bacillus subtilis. DAVID P. FAN AND MARY M. BECKMAN........ 804

Changes in Cell Size and Shape Associated with Changes in the Replication Timeof the Chromosome of Escherichia coli. A. ZARITSKY AND R. H. PRITCHARD. 824

A Paracrystalline Inclusion Formed During Sporulation of Enterotoxin-ProducingStrains of Clostridium perfringens Type A. CHARLES L. DUNCAN, GRETCHEN J.KING, AND WILLIAM R. FRIEBEN.......................................... 845

Stimulation of Cell Division by Croton Oil in Blue-Green Bacteria. LONNIE O'NEALINGRAM AND W. D. FISHER............................................... 874

Inhibition of Spore Outgrowth and Vegetative Growth of Bacillus stearothermophilusby Dipicolinate. MARION L. FIELDS AND HILMER A. FRANK.................. 878

Phosphorylation of Glycerol in Staphylococcus aureus. D. P. RICHEY AND E. C. C. LIN. . 880Site of Catabolite Inhibition of Carbohydrate Metabolism. JAMES F. McGINNIS

AND KENNETH PAIGEN..................................... . ........... 885Influence of Magnesium Ions on Porosity of the Bacillus megaterium Cell Wall and

Membrane. REN9 SCHERRER AND PHILIPP GERHARDT......... ............. 888

EnzymologyPenicillin-Binding Component of Bacillus cereus. RAYMOND F. ATEN AND RICHARD

A.DAY.................................... 587Induction of Superoxide Dismutase by Molecular Oxygen. EUGENE M. GREGORY

AND IRWIN FRIDOVICH.....................................................543Purification and Properties of Threonine Deaminase from the X-1 Isolate of theGenus Thermus. EDWARD H. HIGA AND ROBERT F. RAMALEY................. 556

Chorismate Mutase from Streptomyces aureofaciens: a Heat-Stable Enzyme. HELMUTG6RISCH AND FRANZ LINGENS............................................ 645

Comparison of Acetohydroxy-Acid Synthetases from Two Extreme ThermophilicBacteria. NENA W. CHIN AND JOHN M. TRELA.............................. 674

Membrane Translocation of Mannitol in Escherichia coli Without Phosphorylation.ELLEN SOLOMON, KENJI MIYAI, AND E. C. C. LIN........................... 723

Enzyme Complex Which Couples Glycerol-3-Phosphate Dehydrogenation to FumarateReduction in Escherichia coli. K. MIKI AND E. C. C. LIN..................... 767

Energy-Transducing Adenosine Triphosphatase from Escherichia coli: Purification,Properties, and Inhibition by Antibody. RONALD L. HANSON AND EUGENE P.KENNEDY.............................................................. 772

x*iCONTENTS

CONTENTS

Volume 114 Contents for June Number3Morphology and Ultrastructure

Analysis of Halobacterium halobium Gas Vesicles. MIARK J. KRANTZ AND CLINTON E.BALLOU............................................................ 1058

Model for the Structure of the Shape-M\faintaining Layer of the Escherichia coli CellEnvelope. VOLKMAR BRAUN, HELGA GNIRKE, ULF HENNING, AND KURT REHN... 1264

Ultrastructural Study of Poly-,8-Hydroxybutyrate Granules from Bacillus cereus.WINIFRED F. DUNLOP AND A. W. ROBARDS................................ 1271

Relationship of a Wall-Associated Enzyme with Specific Layers of the Cell Wall of aGram-Negative Bacterium. J. W. COSTERTON.............................. 1281

Isolation of Outer Membranes with an Ordered Array of Surface Subunits from Acine-tobacter. MARGARET J. THORNLEY, AUDREY M. GLAUERT, AND U. B. SLEYTR..... 1294

Isolation and Characterization of Tubules and Plasma M\Iembranes from Cytophagacolumnaris. MICHAEL KUHRT AND JACK L. PATE........................... 1309

Microflora of Soil as Viewed by Freeze-Etching. D. L. BALKWILL AND L. E. CASIDA,JR.............................................................. 1319

Membranes of Bacillus stearothermophilus: Factors Affecting Protoplast Stabilityand Thermostability of Alkaline Phosphatase and Reduced Nicotinamide Ade-nine Dinucleotide Oxidase. CHARLENE WISDOM AND N. E. WELKER... ......... 1336

Surface Structure of Gliding Bacteria After Freeze-Etching. ROBERT P. BURCHARDAND DENNIS T. BROWN................................................. 1351

Conjugation in the Dimorphic Chromomycosis Fungus Phialophora dermatitidis.KAREN B. OUJEZDSKY AND PAUL J. SZANISZLO............................. 1356

Effects of Toluene and Phenethyl Alcohol on the Ultrastructure of Escherichtia coli.C. L. WOLDRINGH...................................................... 1359

Genetics and Molecular Biology

Genetic Determination of the Constitutive Biosynthesis of Phospho-,3-GlucosidaseA in Escherichia coli K-12. I. PRASAD, B. YOUNG, AND S. SCHAEFLER............ 909

Polynucleotide Sequence Relationships Among Japanese and American Strains ofVibrio parahaemolyticus. T. E. STALEY AND R. R. COLWELL.... ............... 916

Comparison of the resAl and polAl Mutations in Isogenic Strains of Escherichia coliK-12. BARRY W. GLICKMAN, CORNELIS A. VAN SLUIS, GERDA VAN DER MAAS, ANDARTHUR RORSCH....................................................... 951

Effect of Cycloheximide on L-Leucine Transport by Penicillium chrysogenum: In-volvement of Calcium. DOUGLAS R. HUNTER, CAROL L. NORBERG, AND IRWINH. SEGEL ............................................................. 956

Transport of Tricarboxylic Acids in Salmonella typhimurium. Ko IMAI, TEIJI IIJIMA,AND TAKEZI HASEGAWA................................................. 961

Replication of Deoxyribonucleic Acid During the Division Cycle of Salmonella typhi-murium. STEPHEN COOPER AND THERESE RUETTINGER..................... 966

Transmissible Plasmid Coding Early Enzymes of Naphthalene Oxidation in Pseudo-monas putida. N. W. DUNN AND I. C. GUNSALUS........................... 974

Regulation of Methionyl-Transfer Ribonucleic Acid Synthetase Formation in Es-cherichia coli and Salmonella typhimurium. ERROL R. ARCHIBOLD AND L. S.WILLIAMS................................... 1007

Deoxyribonucleic Acid Synthesis in recA and recB Derivatives on an Escherichia coliK-12 Strain with a Temperature-Sensitive Deoxyribonucleic Acid Polymerase I.M. MONK, J. KINROSS, AND C. TOWN................................... 1014

Distribution of Ribosomal Ribonucleic Acid Cistrons Among Yeast Chromosomes.SIWO R. DE KLOET................................... 1034

xviii

CONTENTS

Genetic Mutations Affecting the Respiratory Electron-Transport System of thePhotosynthetic Bacterium Rhodopseudomonas capsulata. BARRY MARRS ANDHOWARD GEST.........................................................1045

Regulation of Bacteriochlorophyll Synthesis by Oxygen in Respiratory Mutants ofRhodopseudomonas capsulata. BARRY MARRS AND HOWARD GEST............... 1052

Changes in Isozyme Patterns Between Monokaryons and Dikaryons of a BipolarCoprinus. IAN K. Ross, ELIZABETH M. MARTINI, AND MARILYN THOMAN.... 1083

Deoxyribonucleic Acid Synthesis and Deoxynucleotide Metabolism During Bac-terial Spore Germination. PETER SETLOW................................. 1099

Inhibition of Formation of Escherichia coli Mating Pairs by fl and MS2 Bacterio-phages as Determined with a Coulter Counter. JONATHAN T. Ou............... 1108

Temperature-Sensitive Mutants for the Replication of Plasmids in Escherichia coli:Requirement for Deoxyribonucleic Acid Polymerase I in the Replication of thePlasmid ColEl. DAVID T. KINGSBURY AND DONALD R. HELINSKI........... 1116

Location of the SP02 Attachment Site and the Bryamcin Resistance Marker on theBacillus subtilis Chromosome. ISSAR SMITH AND HARRIET SMITH............. 1138

Isolation of Deoxyribonucleic Acid Methylase iMlutants of Escherichia coli K-12. M.G. MARINUS AND N. RONALD MORRIS.................................... 1143

Restriction and Modification of Bacteriophage S2 in Haemophilus influenzae. RoSAGROMKOVA, JOHN BENDLER, AND SOL GOODGAL............................ 1151

Colicin B: Mode of Action and Inhibition by Enterochelin. SONIA K. GUTERMAN.. 1217Excretion of Enterochelin by exbA and exbB Mutants of Escherichia coli. S. K.

GUTERMAN AND L. DANN .............................................. 1225Mapping of Asporogenous M\Iutations of Bacillus subtilis: a TMinimum Estimate of the

Number of Sporulation Operons. P. J. PIGGOT............................. 1241Use of Constructed Double Mutants for Determining the Temporal Order of Ex-

pression of Sporulation Genes in Bacillus subtilis. J. G. COOTE AND J. MANDEL-STAM . ....................... ......................................... 1254

Molecular Nature of R-Factor Deoxyribonucleic Acid Isolated from Salmonellatyphimurium Minicells. RONALD J. SHEEHY, ANDERSON PERRY, DAVID P.ALLISON, AND ROY CuRTIss III........................................ 1328

Isolation and Characterization of Plasmid Deoxyribonucleic Acid from Strepto-coccus mutans. G. M. DUNNY, N. BIRCH, G. HASCALL, AND D. B. CLEWELL. 1362

Physiology and Metabolism

Extractable Lipids of Gram-Negative Marine Bacteria: Phospholipid Composition.JAMES D. OLIVER AND RITA R. COLWELL................................. 897

S-Adenosyl Methionine-Mediated Repression of Methionine Biosynthetic Enzymesin Saccharomyces cerevisiae. H. CHEREST, Y. SURDIN-KERJAN, J. ANTONIEWSKI,AND H. DE ROBICHON-SZULMAJSTER...................................... 928

Teichoic Acid of a Stabilized L-Form of Streptococcus pyogenes. BOHDAN M1. SLABYJAND CHARLES PANOS.................................................. 934

Ammonium Regulation in Aspergillus nidulans. J. A. PATEMAN, J. R. KINGHORN,ETTA DUNN, AND E. FORBES ........................................... 943

Effect of Serine Hydroxamate on Phospholipid Synthesis in Escherichia coli. LEWISI. PIZER AND JOHN P. MERLIE ......................................... 980

Effect of Iron and Salt on Prodigiosin Synthesis in Serratia marcescens. MELVIN P.SILVERMAN AND ELAINE F. MUNOZ......................... 999

Occurrence of Branched-Chain Hydroxy Fatty Acids in Pseudomonas maltophilia.C. WAYNE MOSS, S. B. SAMUELS, J. LIDDLE, AND R. M. MCKINNEY .......... 1018

Role of Cyclic Adenosine 3',5'-Monophosphate in the In Vivo Expression of theGalactose Operon of Escherichia coli. LUCIA B. ROTHMAN-DENES, JOANNE E.HESSE, AND WOLFGANG EPSTEIN.........................1040

XiX

CONTENTS

Cyclic Adenosine 3',5'-Monophosphate in Escherichia coli. M. J. BUETTNER, EVASPITZ, AND H. V. RICKENBERG.......................................... 1068

Trisporic Acid Biosynthesis in Separate Plus and Minus Cultures of Blakeslea tri-spora: Identification by Mucor Assay of Two Mating-Type-Specific Compo-nents. RICHARD P. SuTTER, DOMENICK A. CAPAGE, THOMAS L. HARRISON, ANDWILLIAM A. KEEN..................................................... 1074

Glutamic Acid Decarboxylase in Spores of Bacillus megaterium and Its Involvementin Spore Germination. CHARLENE WEHE FOERSTER AND HAROLD F. FOERSTER. 1090

Metabolism of Phosphatidylgylcerol, Phosphatidylethanolamine, and Cardiolipinof Bacillus searothernophilus. GEORGE L. CARD............................ 1125

Lipid Synthesis During Sporulation of Saccharomyces cerevisiae. SusAN A. HENRYAND H. 0. HALVORSON................................................. 1158

Osmotic Behavior of Bacterial Protoplasts: Temperature Effects. A. D. EIsENBERGAND T. R. CORNER ................................................... 1177

Oxygen Toxicity and the Superoxide Dismutase. EUGENE M. GREGORY AND IRWINFRIDOVICH ............................................................ 1193

Early Events in Development of Streptococcal Competence. C. GOMEZ LEONARD. . . 1198Nitrate Reduction and the Growth of Veillonella alcalescens. C. B. INDERLIED AND

E . A. DELWICHE ...................................................... 1206Measurement of the Cyanophycin Granule Polypeptide Contained in the Blue-

Green Alga Anabaena cylindria. ROBERT D. SIMON........................ 1213Glucose Fermentation Products of Ruminococcus albus Grown in Continuous Culture

with Vibrio succinogenes: Changes Caused by Interspecies Transfer of H2 . E. L.IANNOTTI, D. KAFKEWITZ, M. J. WOLIN, AND M. P. BRYANT................ 1231

Internal Membrane Control in Azotobacter vinelandii. JACK L. PATE, VINOD K. SHAH,AND WINSTON J. BRILL................................................ 1346

Polyamines of Pseudomonas acidovorans. E. KARRER, R. J. BOSE, AND R. A. J.WARREN............................................................ 1365

EnzymologyRibosomal Ribonucleic Acid-Adenine (N6-) Methylase of Escherichia coli Strain B:

Ionic and Substrate Site Requirements. W. MARSHALL ANDERSON, JR., CHARLESN. REMY, AND JERRY E. SIPE.......................................... 988

Properties of a Fructose-1,6-Diphosphate-Activated Lactate Dehydrogenase fromAcholeplasma laidlawii Type A. HAROLD NEIMARK AND MING C. TUNG........ 1025

Reconstitution of Nitrate Reductase Activity and Formation of Membrane Particlesfrom Cytoplasmic Extracts of Chlorate-Resistant Mutants of Escherichia coli.C. H. MAcGREGOR AND C. A. SCHNAITMAN............................... 1164

Isolation of Two Acetyl Esterases from Extracts of Bacillus subtilis. THOMAS B.HIGERD AND JOHN SPIZIZEN ............................................ 1184

xx