Journal of Clinical PathologyJanuary 1982 Vol. 35 No. 1

ContentsReview articleCervical intraepithelial neoplasia CH BUCKLEY, EB BUTLER, H FOX page 1

Immunoperoxidase study of the secretory immunoglobulin system in colonic neoplasia P ISAACSON page 14

Morphological and mucus secretion criteria for differential diagnosis of solitary ulcer syndrome and non-specificproctitis M EHSANULLAH, MI FILIPE, B GAZZARD page 26

Principal neuropathological and general necropsy findings in 24 renal transplant patients R MORTON, DI GRAHAM,JD BRIGGS, DNH HAMILTON page 31

Computer programs in histopathology record keeping KV SWETTENHAM, C NICKOLS, CL BERRY page 40

Comparative study between biochemical and histological methods and image analysis in liver iron overloadY DEUGNIER, S MARGULES, P BRISSOT, MA DEUGNIER, JC BISCONTE, M BOUREL, B FERRAND page 45

Investigation into paediatric bilirubin analyses in Australia and New Zealand LR WATKINSON, A ST JOHN,LA PENBERTHY page 52

Applicability and significance of salicylate screening in sera of voluntary blood donors: evaluation of twoanalytical methods R SHARON, I FRUTKOFF, G KIDRONI, J MENCZEL page 59

Monoclonal gammopathies in the adult population of Finistere, France JP SALEUN, M VICARIOT, P DEROFF,JF MORIN page 63

A pilot study of the reference values for the commoner haematological and biochemical parameters in Saudinationals T GILBERT SCOTT page 69

1llndium-labelled white blood cells in the diagnosis of Felty's syndrome GG BIRNIE, AS EADIE, CJ HOSIE, NP LUCIE,G WATKINSON page 74

Reaction of the human granulocyte system in successfully treated bacterial bronchopneumonia J KRUSZEWSKI,C SZCZYLIK, W WIKTOR-JEDRZEJCZAK page 77

The United Kingdom national microbiological quality assessment scheme JJS SNELL, JV DE MELLO, PS GARDNERpage 82

System for laboratory proficiency testing in bacteriology: organisation and impact on microbiology laboratoriesin health care facilities funded by the Ontario Government JL WHITBY, WA BLACK, H RICHARDSON, DE WOODpage 94

Enzyme-linked immunosorbent assay for the diagnosis of recent rubella infection YS LIM, CC LAM, SK LAMpage 101

Comparison of ELISA, SPACE, and electron microscopy for the routine diagnosis of rotavirus infectionRA WALL, BJ MELLARS, P LUTON, S BOULDING page 104

Evaluation of the Lumac kit for the detection of bacteriuria by bioluminescence D MACKETT, S KESSOCK-PHILIP,S BASCOMB, CSF EASMON page 107

Technical MethodsDemonstration of immunoproteins in araldite-embedded tissues JUNE GIDDINGS, RL GRIFFIN, AG MaCIVER page 111

A rapid and accurate differential centrifugation method for platelet counts S COUSINS, SM LEWIS page 114

Letters to the Editor page 117

Book Reviews page 120

Notices page 124

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ContentsNo 1 JANUARY 1982

PageReview articleCervical intraepitheial neoplasia CH BUCKLEY, EB BUTLER, HFOX. 1Immunoperoxidase study of the secretory immunoglobulin system in colonic neoplasia P ISAACSON ......................... 14Morphological and mucus secretion criteria for differential diagnosis of solitary ulcer syndrome and non-specific proctitisM EHSANULLAH, MI FIUPE, B GAZZARD ................................................................................ 26Principal neuropathological and general necropsy findings in 24 renal transplant patients R MORTON, DI GRAHAM, JD BRIGGS, DNHHAMlLTON ........................................................................................................ 31Computer programs in histopathology record keeping KV SWETTENHAM, C NICKOLS, CL BERRY ............................... 40Comparative study between biochemical and histological methods and image analysis in liver iron overload Y DEUGNIER,

S MARGULES, P BRISSOT, MA DEUGNIER, JC BISCONTE, M BOUREL, B FERRAND .................................................. 45Investigation into paediatric bilirubin analyses in Australia and New Zealand LR WATKINSON, A ST JOHN, LA PENBERTHY ....... 52Applicability and significance of salicylate screening in sera of voluntary blood donors: evaluation of two analytical methodsR SHARON, I FRUTKOFF, G KIDRONI, J MENCZEL .......................................................................... 59Monoclonal gammopathies in the adult population oI Finistere, France JP SALEUN, M vICARIOT, P DEROFF, JF MORIN .... ....... 63A pilot study of the reference values for the oommoner haematological and biochemical parameters in Saudi nationalsT GILBERT SCOTT .................................................................................................... 69l'Indium-labelled white blood cells in the diagnosis of Felty's syndrome GG BIRNIE, AS EADIE, CJ HOSIE, NP LUCIE, G WATIUNSON 74Reaction of the human granulocyte system in successfully treated bacterial bronchopneumonia J KRUSZEWSKI, C SZCZYUK,W WIKTOR-JEDRZEJCZAK............................................................................................. 77The United Kingdom national microbiological quality assessment scheme JJS SNELL, JV DE MELLO, PS GARDNER ............... 82System for laboratory proficiency testing in bacteriology: organisation and impact on microbiology laboratories in health care facilitiesfunded by the Ontario Government JL WHITBY, WA BLACK, H RICHARDSON, De WOOD ...................................... 94Enzyme-linked immunosorbent assay for the diagnosis of recent rubella infection YS LIM, CC LAM, SK LAM................... 101Comparison of ELISA, SPACE, and electron microscopy for the routine diagnosis of rotavirus infection RA WALL, BJ MELLARS,P LUTON, S BOULDING .............................................. .................... .................... 104Evaluation of the Lumac kit for the detection of bacteriuria by bioluminescence D MACKErr, S KESSOCK-PHLIP, S BASCOMB,CSF EASMON ....................................................................................................... 107Techmcal methodsDemonstration of immunoproteins in Araldite-embedded tissues JUNE GIDDINGS, RL GRIFFIN, AG MaCIVER..................... 1A rapid and acccurate differential centrifugation method for platelet counts S COUSINS, SM LEWIS .......................... 114Letters to the Editor .............................................................................................. 117Book reviews ..................................................................................................... 120Notices ........................................................................................................... 124

No 2 FEBRUARY 1982Page

Quantitative bone histology in 38 patients with advanced renal failure JB EASTWOOD ..................................... 1225Quantitative analysis of non-Hodgkin's lymphoma CR ABBOTr, RW BLEWITT, CC BIRD ...................................... 135Detection of surface immunoglobulins of human lymphoid cells: a comparative study of live and fixed cells using a directimmunoperoxidase procedure G LAURENT, MARIE FRANCOISE GOURDIN, F REYES ............................................ 139Immunohistological study of human lungs by immunoperoxidase technique B FOX, S SHOUSHA, KR JAMES, GILUAN C MILLER .... 144Practical use of a word processor in a histopathology laboratory JC BRIGGS, NBN IBRAHIM, I MACKINTOSH, D NORRIS .... ........ 151Macrophage origin of Reed-Stemnberg cells: an immunohistochemical study sv PAYNE, DH WRIGHT, KJM JONES, MA JUDD ....... 159Lysosomal localisation of parallel tubular arrays in chronic lymphocytic leukaemia of T cell origin: an ultrastructural cytochemicalstudy F CAPRON, JY PERROT, C BOUCHEIX, M REYNES, V TRICOTTET, A BERNADOU, J DIEBOLD .................................... 167Iron granules in plasma cells- MARGARET K COOK, M MADDEN ............................................................ 172Granulocyte chemotaxis: multiple assay screening using a raft technique U JAYASWAL, S ROPER, S ROATH ..... ................ 182Bone marrow processing and cryopreservation DC LINCH, LU KNOTT, KG PATTERSON, DA COWAN, PG HARPER ..... .............. 186Cell structure and percent viability by a slide centrifuge technique MARGARET G FITZGERALD, CS HOSKING ..... ................ 191Ascorbate status and fibrinogen concentrations after cerebrovascular accident R HUME, BD VALLANCE, MM MUIR .... .......... 195Circulating CK-MB and CK-BB isoenzymes after gastrointestinal surgery SH TSUNG ..................................... 200Total CK activity and isoenzyme patterns in normal and neoplastic tissue of gastrointestinal tract SH TSUNG ..... ............ 204Serum glutamate dehydrogenase is not a reliable marker of liver cell necrosis in alcoholics WJ JENKINS, SB ROSALKI, Y FOO, PJSCHEUER, E NEMESANSZKY, S SHERLOCK ............................................................................... 207Diagnosis of Legionella pneunophila infections by means of formolised yolk sac antigens TG HARIMSON, AG TAYLOR ........... 211Failure of the fluorescent antibody reaction to identify penidlllinase-producing gonococci SHEENA A WArnrNS, R DAWN ANDERSON . 215Osteomyelitis and septic arthritis caused by KingeUa kingae JM DAVIS, MM PEL .......................................... 219Swabs and swab-transport media kits in the isolation of upper respiratory bacteria PW ROSS, CG CUMMING, H LOUGH .... ...... 223Comparative study of three tests (dye test, indirect haemagglutination test, latex agglutination test) for the detection of antibodiesto Toxoplasma gondii in human sera AH BALFOURt, DO FLECK, HPA HUGHES, D SHAP ........................................ 228Appraisal of Anotox, a new anaerobic atmospheric detoxifying agent for use in anaerobic cabinets JS BRAZIER .... .......... 233Technical methodsTissue carbohydrate identification by the use of lectins KP WEST, HA PLATrS, A FLETCHER, F WALKER ..... .................... 239A method for transmission and scanning electron microscopy of undecalcified human bone marrow biopsy specimens usingcryofracture F KUTO, T NAGAOKA, M HAYASHI, Y HIRASAWA, H TOKUHIRO .................................................. 240Letters to the Editor ............................................................................................... 243Book reviews ................................................................................................... 246

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No 3 MARCH 1982Page

Presidential address-1981Clinical pathologists-a threatened species? WD LINSELL ............................................................... 249Review articleBone biopsy in haematological disorders R BURKHARDT, B FRISCH, R BART ............................................... 257Intracellular immunoglobulin distribution of bone marrow plasma cells as a diagnostic aid for prmary amyloidosis c THIELEMANS,W AELBRECHT, D VERBEELEN, G SOMERS, M DE WAELE, B VAN CAMP ......................................................... 285

Effect of height and weight on the in vivo recovery of transfused factor VIII C A ARONSTAM, DS MCLELLAN, M WASSEF, PS MBATHA 289Carboxyhaemoglobin: a possible reference material for haemoglobin assay JM RIDEOUT, A LOUDERBACK .... ................. 292Use of buffered hypochlorite solution for disinfecting fibrescopes D COATES, JANET E DEATH ................... ............ 296Comparison of antisera in the fluorescent antibody test for detection of Bacteroides spp in clinical specimens A WILLS, EUZABETHTAYLOR, ANNAUSA PANTOSTI, I PHILUPS, SOAD TABAQCHAU ............................................................... 304

Isolation of Bacteroides ureolyticus (B corrodens) from clinical infections BI DUERDEN, KW BENNET, JUUA FAULKNER .... ...... 309How important are the dermatophytes? A clinical and laboratory investigation DG DAVIES, J DEIGHTON, WD PATERSON .... ..... 313Characterisation of liver membrane autoantibodies determined by indirect immunofluorescence u TAGE-JENSEN, H PERMIN, KKROGSGAARD ...................................................................................................... 315

Soya protein antibodies in man: their occurrence and possible relevance in coeliac disease MR HAENEY, BJF GOODWIN, MEJ BARRATT,N MIKE, P ASQUITH ................................................................................................. 319Luminal ions and short chain fatty acids as markers of functional activity of the mucosa in ulcerative colitis WEW ROEDIGER, MHEYWORTH, P WILLOUGHBY, J PIRIS, A MOORE, SC TRUELOVE ............................................................... 323

Combined immunological and histochemical analysis of skin and lymph node lesions in histiocytosis X JA THOMAS, G JANOSSY, MCHILOSI, J PRITCHARD, JR PINCOTT ..................................................................................... 327

Collagenous colitis: a distinctive and potentially reversible disorder AS PIETERSE, R HECKER, R ROWLAND .... ................. 338Medical audit of rectal biopsy diagnosis of inflammatory bowel disease JV FREI, BC MORSON ................................ 341Feulgen-hydrolysis profiles in cells exfoliated from the cervix uteri: a potential aid in the diagnosis of malignancy JA MILLETT, OANHUSAIN, L BITENSKY, J CHAYEN ....................................................................................... 345

Urine cytology in patients with calculi WILMA HIGHMAN, EUZABETH WILSON .............................................. 350Technical methodsNasal brushing for the study of ciliary ultrastructure J RUTLAND, ANN DEWAR, THERESA COX, P COLE ..... .................... 357A new bone marrow biopsy needile with core securing device A ISLAM .................................................. 359Letters to the Editor .............................................................................................. 364Book reviews............................................................................................... 367

No 4 APRIL 1982Review article PageMonoclonal antibodies in oncology K SIKORA ............ ............................................................ 369Mouse red cell rosette formation and the colchicine sensitivity test: relative usefulness in the differential diagnosis of chroniclymphocytic leukaemia and B lymphocytic lymphoma PENELOPE J SUGDEN, JS LULLEYMAN .................................. 376Circulating immune complexes and complement concentrations in patients with alcoholic liver disease C GLUUD, H JANS ...... 380Protein contents and binding modes of immunoglobulin-amylase complexes H OHTANI, K SAKAGUCHI, M SAITO................ 385Synovial fluid lactic acid measurement in the diagnosis and management of septic arthritis T RIORDAN, D DOYLE, SOAD TABAQCHAU . 390Incidence of hypercalcaemia and primary hyperparathyroidism in relation to the biochemical profile JS HARROP, JE BAILEY, JSWOODHEAD ........................................................................................................ 395Biochemical assessment of histochemical methods for oestrogen receptor localisation JCE UNDERWOOD, E SHER, M REED, JA EISMAN,TJ R1IN ................................................................................................. 401Macroscopic enzyme histochemistry in myocardial infarction: use of coenzyme, cyanide, and phenazine methosulphate NWDERIAS, CWM ADAMS ................................................................................................ 407Macroscopic enzyme histochemistry in myocardial infarction: artefactual nature of the creatine phosphokinase reaction NWDERIAS, CWM ADAMS ............................................................................................... 410Accuracy of morphological diagnosis of lung cancer in a department of respiratory medicine MD CLEE, HELEN LD DUGUID, DJMSINCLAIR ......................................................................................................... 414Bilateral gonadoblastoma/dysgerminoma in a 46 XY individual: case report with hormonal studies RA FISHER, R SALM, RW SPENCER 420Combined light and electron microscope in routine histopathology S JONES, SK CHAPMAN, PR CROCKER, GINA CARSON, DA LEVISON 425Use of orcein in detecting hepatitis B antigen in paraffin sections of liver P KIRKPATRICK ............ ...................... 430Polyvalent heat-killed antigen for the diagnosis of infection with Legionella pneumophila Ri FALLON, WH ABRAHAM .. . 434Serological characterisation of Ureaplasma urealyticum strains by enzyme-linked immunosorbent assay (ELISA) H TURUNEN, PLEINIKKI, ELU JANSSON ............................................................................................. 439A microcomputer system for the collection and analysis of antibiotic sensitivity test data DMCG CLARKSON, KL THOMAS,G WYNNE-WILIUAS ................................................................................................ 444Comparative epidemiology of gentamicin-resistant enterobacteria: persistence of carriage and infection CA HART, MARJORIE FGIBSON ............................................................................................................ 452Evaluation of Fastidious Anaerobe Broth as a blood culture medium LEELA A GANGULI, LJ TURTON, GS TILLOTSON .... ........ 458A selective medium for isolating Campylobacter jejunilcoli FJ BOLTON, L ROBERTSON ...................................... 462Technical methodsAn evaluation of the API-20 STREP system GS TILLOTSON ............................................................ 468Use of FITC-protein A in place of fluorescein-conjugated anti-gammaglobulins for rapid virus diagnosis by immunofluores-cence DJ WOOD, G CORBIT ......................................................................................... 472Letters to the Editor .............................................................................................. 476Book reviews .......................................................................................... .... 479

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No 5 MAY 1982Page

Binding of serum ferritin to concanavalin A in patients with iron overload and with chronic liver disease RWG CHAPMAN, A GORMAN,M LAUUCHT, MAM HUSSAIN, SHEILA SHERLOCK, AV HOFFBRAND ...... ........................ . .............................. 481Effect of ascorbic acid deficiency on serum ferritin concentration in patients with ,8-thalassaemia major and iron overload RWGCHAPMAN, MAM HUSSAIN, A GORMAN, M LAULICHT, DINA POLTIS, DM FLYNN, SHEILA SHERLOCK, AV HOFFBRAND.................... 487Ratio of blood and marrow-derived cells in bone marrow transplantation HEM KAY, HILARY LUMLEY, HMCD CUNK, RL POWLES .. 492Distribution pattern of lysosomal granules in fibroblasts of the Chediak-Higashi syndrome K ABE, S ARASHIMA, M HONMA ...... 496Effect of total dose infusion of iron dextran on the storage iron content of the human placenta BB BAUMINGER, G WALTERS, JTWHICHER, AB DUKE ................................................................................................ 502Elution of "'Indium from reticuloendotheial cells AM PETERS, I KLONIZAKIS, JP LAVENDER, SM LEWIS ........................ 507Malignant histiocytosis of the intestine: report of three cases with immunological and cytochemical analysis P ISAACSON, DB JONES,MJ SWORN, DH WRIGHT ....................5........................................................................510Studies of intestinal lymphoid tissue. IV - The predictive value of raised mitotic indices among jejunal epithelial lymphocytes in thediagnosis of gluten-sensitive enteropathy MN MARSH .................................................................. 517Ovarian tumours of Wolffian or allied nature: their place in ovarian oncology PE HUGHESDON ............................. 526Pathological evaluation of computed tomography images of lungs ROSEMARY CODDINGTON, SL MERA, PR GODDARD, JWB BRADFIELD 536Primary biliary cirrhosis: an increased incidence of extrahepatic malignancies? PR MILLS, P BOYLE, EMM QUIGLEY, GG BIRNIE, FJARRETT, G WATKINSON, RNM macsWEEN ............. .................................................................. 541Significance of the site of origin of pancreatic exocrine adenocarcinoma TG ALLEN-MERSH ................................. 544Comparison of the diagnostic value of serum pancreatic isoamylase and immunoreactive trypsin measurement in patients withcystic fibrosis RC BROWN, DM CHALMERS, VL ROWE, I KELLEHER, IM LITTLEWOOD, MS LOSOWSKY ............................... 547Comparison of culture and microscopy in the diagnosis of Gardnerella vaginalis infection CA ISON, SG DAWSON, J HILTON, GWCSONKA, CSF EASMON ................................................................................................ 550A transport method for swab specimens submitted for aerobic and anaerobic bacteriology M ALFA, A LEE ..... .............. 555Clostridium difficile and cytotoxin in routine faecal specimens JQ NASH, B CHATTOPADHYAY, JOYCE HONEYCOMBE, SOAD TABAQCHALI 561A simple, rapid ELISA method for the detection of DNA antibodies RP STOKES, A CORDWELL, RA THOMPSON .... ............ 566Technical methodsA celloidin bag for the histological preparation of cytologic material G BUSSOLATI .............. .......................... 54An improved method for sequential light and scanning electron microscopy of the same cell using localising microcoverslips KMSALEH, PG TONER, KE CARR, HE HUGHES ............................................................................... 576Letters to the Editor .............................................................................................. 580Book reviews ................................................................................................. 584Some new titles ................................................................................................. 584

No 6 JUNE 1982Page

Morphological characteristics and distribution patterns of epithelial cells in the cytological diagnosis of gastric cancer JENNIFER AYOUNG, HELENA E HUGHES, DJ HOLE .................................................................................. 585Heterotopic gastric mucosa in the duodenum AM LESSELLS, DF MARTIN .................................................. 591Increased tissue concentrations of histamine in the duodenal mucosa of children with coeliac disease DN CHALLACOMBE, PDDAWKINS ......................................................................................................... 596Biopsy findings in malignant histiocytosis presenting as lethal midline granuloma K AOZASA ............................... 599Villous atrophy with crypt hyperplasia in malignant histiocytosis of the nose K AOZASA ................................... 606Primary endocrine carcinoma of the parotid salivary gland association with a lung carcinoid: a possible new association v EUSEBI, SPILERI, L USELUNI, A GRASSIGU, C CAPELLA ........ .............. ..................................................... 611Systemic mastocytosis, myelofibrosis and portal hypertension AH SAWERS, J DAVSON, JOAN BRAGANZA, CG GEARY .... .......... 617A flexible microprocessor system for the measurement of cell'size JOAN M ROUND, DA JONES, RHT EDWARDS ..... ............. 620Value of plasma calcium, phosphate, and alkaline phosphatase measurements in the diagnosis of histological osteomalacia HPEACH, JULIET E COMPSTON, S VEDI, LWL HORTON ........ ................................................................ 625Interrelations between cerebrospinal fluid and plasma inorganic ions and glucose in patients with chronic renal failure IF PYE, GMABER.............................................................................................................. 631Serum gamma-glutamyltransferase and alkaline phosphatase in rheumatoid arthritis RJ SPOONER, DH SMITH, DOROTHY BEDFORD, PRBECK ............................................................................................................. 638A rapid method for staining inclusions of Chlamydia psiuaci and Chlamydia trachomatis RM WOODLAND, JAYSHREE MALAM, SDAROUGAR ........................................................................................................ 642Determination of specific IgA antibodies to varicella zoster virus by immunoperoxidase assay H HAIKIN, I SAROV ............. 645Evaluation of a micromethod gallery (API Staph) for the identification of staphylococci and micrococci RR MARPLES, IFRICHARDSON ...................................................................................................... 650An ELISA test for the detection of antibodies to Legionella pneumophila TG WREGHIrr, J NAGINGTON, J GRAY .... ............ 657Antithrombin III and fibrinogen degradation product (fragment E) in diabetic nephropathy V CHAN, CK YEUNG, TK CHAN ..... 661Acquired dysfibrinogenaemia in liver disease JL FRANCIS, DJ ARtMTRONG ................................................. 667Anticoagulants antibodies in the synovial membranes of patients suffering from haemophilia, rheumatoid arthritis and otherrheumatic disorders SG RAINSFORD, A ARONSTAM, EJ HOLBOROW, DS MCLELLAN ............................................ 673Comparative study of the Fenwal and Pall microaggregate filters used for the production of leucocyte-poor blood JG TRELEAVEN,KG PATrERSON, I BLAGDON .......................................................................................... 681Comparison of lymphocyte transformation and macrophage migration inhibition tests in the detection of beryllium hypersen-sitivity WR WILUAMS, W JONES WILUAMS.684

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Technical methods Page"Cell-blocke' technique for fine needile aspiration biopsy PH KANAUCHOW, RE BONN ...................................... 688Ferrography-a new method for isolation of particles from biological fluids JA HUNTE, GH MILLS, RD STURROCK .............. 689Letters to the Editor .............................................................................................. 690

No 7 JULY 1982Page

Hepatitis B and A virus antibodies in alchoholic steatosis and cirrhosis c GOUD, J ALDERSHVILE, J HENRIKSEN, P KRYGER, LMATIHESEN ....................................................................................................... 693Problems in the disinfection of class 1 microbiology safety cabinets PH EVERAU, CA MORRIS, PR OUVER, JF BECKER .... ......... 698Can direct gas-liquid chromatography of clinical samples detect specific ornisMS? B WATT, PAMELA A GEDDES, OLGA A GREENAN,SUSAN K NAMER, A MIfCIELL ........................................................................................ 706Gas-liquid chromatography in the diagnosis of anaerobic infections: a three year experience B WATr, PAMELA A GEDDES, OLGA AGREENAN, SUSAN K NAPIER, A MfTCHELL ............................................................................... 709Diagnosis of bacteraemia by automated head-space capilary gas chromatogaphy L LARSSON, P-A MARDH, G ODHAM, M-LCARISSON ......................................................................................................... 715Evaluation of an improved Streptex kit for the grouping of beta-haemolytic streptococci by agglutination D CASTLE, SKESSOCK-PHIUP, CSF EASMON ........................................................................................ 719Fibronectin in exudative pleural effusions iATn KLOCKA E, T PEitERSSON, T VARTIO, H RISKA, A VAHERI ..... ................. 723Effect of treatment with 17 a-alkylated androgens on C4 conversion products in hereditary angioedema studies by crossedimmunoelectrophoresis L BERGAMASCINI, M CICAD, ANTOIA TUCI, A AGOSTONI ...................................... 728Hypercalcaemia due to the coexistence of parathyroid adenoma and myelomatoss tM PRANCS, AG BYNOE, C GRAY .... ....... 732Glycosylated haemoglobin in chronic renal failure and after renal transplataton D FREEDMAN, P DANDONA, O FERNANDO, IFMOORHEAD..... 737Platelet inhibition with Ticlopidine in atherosclerotic i audication A AUKLAND, RA HURLOW, A.GEORGE, J STUAR.T. 740Occurrence of infection with a parvovirus-like agent in children with sickle cell anaemia during a two-year period MJ ANDERSON,LR DAVIS, J HODGSON, SE JONES, L MURTAZA, JR PATISON, CE SaUDoM, W.............................................w.744Reaction against liver cells by human antiactin antibodies puified by affiy chromatography KIRSTEN RIISOM, H DIEDERICHSEN,INGER ANDERSEN .................................................................................................. 750Influence of the gallbladder on serum bile acids ca PENNINGrON, PR ROSs, ID IaIOUCH ................................... 754Five year follow-up study of gastritis MC ORMISON, MWL GEAR, BW CODIUNG ................................................ 757Comparison of the Trucut and Surecut liver biopsy needles ER UITLEWOOD, f GLMOE, IM MURRY-LYON, KR STEPHENS, FJPARADINAS................................................................................................. 761Somatostatin: a paracrine contribution to hypothyroidism in Hashimoto's thyroiditis AP DHILLON, J RODE, A LEATHEM, LUCIENNEPAPADAKI ................................................................................................ 764Ultrastructural features of the osteoclasts from Paget's disease of bone in relation to a viral aetiology L HARVEY, T GRAY, MNCBENErON, DL DOUGLAS, JA KAIS, RGG RUSSELL ......................................................................... 771Adamantinoma of long bones: chical, pathological and ultrastructural features AS HErE, PS SMITH, J MCCLURE .... ........ 780Technicl methodsAssay of ,B-lactam antibiotics when used therapeutically in combination with aminoglycosdes BH POIMA ..... .............. 787Congo blue: a rapid stain for elastic fibres JD DAVIES, EW YOUNG ....................................................... 789Letters to the Editor .............................................................................................. 791Book reviews ................................................................................................. 794Notices ................................................................................................ 796Some new titles ................................................................................................ 796

No 8 AUGUST 1982Review artck PageOxygen transport in chronic hypoxic lung disease Dc FLENY ......................................................... 797Urine cyclic nucleotide concentrations in cancer and other conditions; cyclic GMP: a potential market for cancer treatment GATURlNER, RD ELUS, D GUlRIE, AL LATNER, JM MONAGHAN, WM ROSS, AW SKIILEN, RG WISON .................................. 800Measurement of the fasting urinary hydroxyproline: creatinine ratio in normal adults and its variations with age and seX AHODGOKNSON, T THOMPSON .......................................................................................... 807Hyperplastic parathyroiditis- a new autoimmune disease? IF BOYCE, Vi DOHEY, G MORIMER ............................ 812Lysozyme in chronic liver disease: a biochemical and histological study s MANIFOLD, FM BISHOP, P CLOKE, DR TRIGER, JCEUNDERWOOD.1...................................................... 815Distribution of plasma cells in normal rectal mucosa RCF LEONARD, ICM MACLENNAN ...................................... 820Angiodysplaias of the colon Di pOUNDER, R ROWLAND, AS PiTEsE, aFRMAN, R Hummr ................................. 824Pathology of colorectal adenomas: a colonoscopic survey F KONISH, DC MOSON ......................................... 830Fibroma of tendon sheath Ps Sm, As EsE, i mccLu.........sMiTH,ASP.................................................. 842Recurring digital fibroma G MORIR, AAM GIBSON....................................................................849Segmental lymph-node infarction after fine-needle aspiration JD DAVIS, A WEBB ......................................... 855A new manifestation of thrombocytopenia: myocardial haemorrhage with symptomatic arrhythmia Pi WYLD, S BECK, DN SLATER . 858Cytogenetic study in acute myeloid leukaemia using peripheral blood samples sent by post YS U, FGI HAYHOE .... ........... 861C-reactive protein concentration as a guide to antibiotic therapy in acute leukaemia K? SCHOFIELD, P VOULGARI, DI GOZZARD, MsLEYLAND, NJ BEECHNG, J STUART ............................... 866Morphological and functional disturbanc of platekts induced by cry eservation H AYIHOON, EGD TUDDENNAM, RA HUTTON 870

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Biotyping of Enterobacter cloacae DC OLD ...................... ............................................... 875Value of the minimum bactericidal concentration of antibiotics in the management of a case of recurrent Streptococcus bowssepticaemia LENA ROBINSON, K FONSECA.879A microcomputer system for clinical bacteriology: experience of. 12 months' trial Ri COURCOL, M ROUSSEL-DELVALLEZ, OR MARTIN 881Frequency of Candida albicans serotypes in patients with denture-induced stomatitis and in normal denture wearers MV MARTIN,DJ LAMB.888Enzyme-linked immunosorbent assay (ELISA) using antibody class capture for the detection of antitoxoplasma IgM RA PAYNE, MISAAC, JANET M FRANCIS.892Technical methodsStaining properties of human intestinal mucosal mast cells after glutaraldehyde fixation s STROBEL, M HASAN, ANNE FERGUSON 897Manual screening for immune antitetanus antibodies by means of latex coated with tetanus toxoid JR BOOTH, PA NUTrALL 899Letters to the editor.901Notices.904Some new titles.904Book reviews.905

No 9 SEPTEMBER 1982Page

Review ArticleThe maternal autopsy DI RUSHTON, Ip DAWSON.909Plasma carcinoembryonic antigen concentrations and immunohistochemical patterns of epithelial marker antigens in patients withlarge bowel carcinoma TO ROGNUM, K ELGJO, P BRANDTZAEO, H 0RJASAETER, A BERGAN.922Morphological and immunohistochemical findings in upper gastrointestinal biopsies of patients with Crohn's disease of the ileumand colon JP VAN SPREHUWEL, I LINDEMAN, AM VAN DER WAL, I WETERMAN, J KREUNING, CJLM MEIJER.934Granulomatous masitis: a report of seven cases A FLETCHER, IM MAGRATH, RH RIDDELL, IC TALBOT.941Malignant fibrous histiocytomas of salivary glands E BENJAMIN, S WELLS, H POX, N REEVE, F KNOX.946Study of nuclear diameters in non-Hodgkin's lymphomas XCROCKER, EL JONES, RC CURRAN.954Neutrophil granuocytic cell antigen defined by a monoclonal antibody- its distribution within normal haemic and non-haemictissue HW SCIENLE, H STEIN, W MOLLER-RUCHHOLTZ .... .............................................................. 959Relation of neutrophil alkaline phosphatase activity to Fc-IgG receptor development in human blood and bone marrow cs SCOTF,RJ CORDINGLEY, BE ROBERTS, AG BYNOE, D HOUGH.967Monocyte function in cirrhosis G HOLDSTOCK, B LESUE, S HILL, AR TANNER, R WRIGHT.972A simple quantitative assay of tissue plasminogen activator UNDA DE COSSART, RW MARCUSON.980Acidosis and severe megaloblastic anaemia uI FRANKUN, PDA KERNOFF, D ISMERWOOD, J LEEK, DB MORGAN.984The test request form: a neglected route for communication between the physician and the clinical chemist? AR HENDESON . . 986Intrapartum hypoxia: the association between neurological assessment of damage and abnormal excretion of ATP metabolites RAHARKNESS, AGL WHITELAW, RJ SIMMONDS.999Basic isoferritin and hypercalcaemia in renal cell carcinoma Gi MUT!, Ti HAMBUN, J STEVES.1008Radioimmunoasay of serum glycocholic acid, standard laboratory tests of liver function and liver biopsy findings: comparativestudy of children with liver disease A MATSUI, HT PSACHAROPOULOS, AP MOWAT, B PORTMANN, GM MURPHY.1011The Howie code: is the price of safety too high? DR COHEN.1018Methods for the routine characterisation of isolates of Haemophilus PM ZADIK.1024A rapid microagglutination test for the diagnosis of LegioneUa pneumophila (serogroup 1) infection TG HARRISON, AG TAYLOR . 1028Technical methodHistological demonstration of platelet thrombi with a double silver impregnation argyrophil stain DN SLATER, G ANDERSON, JFMARTIN.1032Letters to the Editor.1034Notices.1037Some newtitles. 1038Book reviews.1038

No 10 OCTOBER 1982Page

Review ArticleSome aspects of the metastatic process RtLCAR .................................................................... 1041Value of external quality assessment of the technical aspects of histopathology WT BARR, ED WILUAMS ..... ................. 1050Morphometric study of liver cell nuclei in hepatomas using an interactive computer technique: (i) nuclear size and shape R JAGOE,C SOWTER, SHEILA DANDY, G SLAVIN.1057Morphometrical analysis of urothelial cells in voided urine of patients with low grade and high grade bladder tumours ECM OOMS,PJH KURVER, MATEHLDE E BOON.1063A quantitative study of a-naphthyl acetate esterase-positive cells in non-Hodgkin's lymphomas and reactive lymph nodesJ CROCKER, EL JONES, RC CURRAN.1066Wilms' tumour and renal dysplasia: an hypothesis HB MARSDEN, W LAWLER.1069Unusual subcutaneous mixed tumour exhibiting adipose, fibroblastic, and epithelial components PS SMITH, I MCCLURE.1074Micronodular adrenal disease: a light and electron microscopic study PS HASELTON, HH AU, C ANFIELD, CG BEARDWELL, S SHALET . 1078

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6-Thioguanine as a cause of toxic veno-occlusive disease of the liver MB SATll, K WEINBREN, EC GoRDON-SMIITH .... ........... 1086Improved double immunoenzyme labelling using alkaline phosphatase and horseradish peroxidase NJ MAUK, ME DAYMON ..... 1092Guidelines for the evaluation of instruments used in haematology laboratories NK SHINTON, IM ENGLAND, DA KENNEDY ......... 1095A new case of congenital dyserythropoietic anaemia, type III: studies of the cell cycle distribution and ultrastructure of erythro-blasts and of nucleic acid synthesis in marrow cells SN WICKRAMASINGHE, TE PARRY, C WILUAMS, AN BOND, MADELEINE HUGHES, SALLYCROOK...........1103CROK.................................................................................................................... 10Assessment of a radioisotopic assay for vitamin B12 using an intrinsic factor preparation with R proteins blocked by vitamin B12analogues BARBARA BAIN, GN BROOM, JACKIE WOODSIDE, RA LTWNCZUK, SN WICKRAMASNGHE ............................... 1110Three step purification of Clq by DNA precipitation, ion exchange and lectin affinity chromatography M RHEN, E UNDER ..... 1114Antinuclear antibody - negative systemic lupus erythematosus - how common? KC MCHARDY, CHW HORNE, JAN RENNIE .... .... 1118Development and evaluation of an enzyme-linked immunosorbent assay (ELISA), using chlamydial group antigen, to detectantibodies to Chlamydia trachomads RT EVANS, D TAYLOR-ROBINSON ...................................................... 1122Enzyme-linked immunosorbent assay (ELISA) for detection of specific IgA antibodies to mumps virus B HALIVY, I SAOV .... 1129Rapid enzyme-linked immunosorbent assay (ELISA) for Aspergilus fismigatus antibodies MD RICHARDSON, JUDITH M STUBBINS,DW WARNOCK ...............................13....................................................................1134Detection of antibodies to tetanus toxoid: comparison of a direct haemagglutination method with a radioimmunoassay AS WANG,GF BURNS, U KRONBORG, IlR MACKAY ................................................................................... 1138Comparison of a homemade blood culture broth containing a papain digest of liver, with four commercially available media for theisolation of anaerobes from simulated paediatric blood cultures GH HUNT, ELIZABETH H PRICE ...... ........................ 1142Reciprocal antimicrobial synergism between Escheichia coli and Bacteroidesfragilis in the presence of metronidazole F SORIANO,MC PONTE, MC GASPAR ............................................................................................... 1150Acquisition of new genes by oral Neisseria CATHERINE ISON, AA GLYNN, SHOSHANA BASCOMB ....... ......................... 1153The use of an anaerobic incubator for the isolation of anaerobes from clinical samples PL BERRY, E TAYLOR, I PHILUPS .... .... 1158Technical methodHistological localisation of mammographic abnormalities in breast biopsy specimens GWH STAMP, IW MCDICKEN, GHWHITEHOUSE ...................................................................................................... 1163Letters to the Editor .............................................................................................. 1166Book reviews ................................................................................................. 1167Notices............................................................................................... 1168

No 11 NOVEMBER 1982Page

An antibiotic susceptibility testing trial organised as part of the United Kingdom national external microbiological quality assess-ment scheme ns SNELL, DFJ BROWN, PS GARDNER ....... .............................................................. 1169Sensitivity tests with cephalosporins PAMELA H WATERWORTH ........................................................... 1177Laboratory diagnosis of peritonitis in patients undergoing continuous ambulatory peritoneal dialysis PATRICIA FENTON .... .... 1181Bacteriuria in patients undergoing prostatectomy MELANIE WILLUAMS, lU HOLE ........................................... 1185A prospective study ot cytomegalovirus and herpes simplex virus disease in renal transplant recipients DP WALKER, M LONGSON, NPMALLICK, RWG JOHNSON ............................................................................................. 1190Estimation of lymphocyte percentage and number on the Coulter Counter, Model S Plus Phase II JM ENGLAND, MC CHETTY, PM DESILVA .......................................... .......... ....................................... 1194Acute myelomonocytic leukaemia presenting as xanthomatous skin eruption JR O'DNNELL, P TANSEY, P CHUNG, AK BURNETT, JTHOMSON, GA MCDONAD ............................................................................................ 1200Serum ferritin in normal individuals and in patients with malignant lymphoma and chronic renal failure measured with sevendifferent commercial immunoassay techniques ANN GRAIL, BW HANCOCK, PAUUNE M HARRISON .............................. 1204Alpha-1-antitrypsin phenotypes in malignant lymphoma F CALLEA, G MASSI, C DE WOLF-PEETERS, C UEVENS, VJ DESMET .... .... 1213T and B lymphocyte markers in effusions of patients with non-Hodgkin's lymphoma AS KRAJEWSKI, AE DEWAR, EF RAMAGE .... 1216Enzyme histochemical analysis on cryosat sections of human bone marrow M CHILOSI, G PIZZOLO, G JANOSSY, MARGARITA BOFILL, LFIORE-DONA ................................................................................................. 1220Immunological localisation of B-thrombogulin and platelet factor 4 in human megakaryocytes and platelets KATHRYN M MCLAREN,DS PEPPER................................................................................................. 1227A simple method for the separation and quantification of urinary porphyrins DT LEAHY, TG BRIEN ..........................-1232Antenatal diagnosis of steroid sulphatase deficiency: case report and literature survey RA SHERWOOD, BF ROCKS .... .......... 1236A prospective comparison of the fluorescein dilaurate test with the secretin-CCK test for pancreatic exocrine function EJS BOYD,JGR CUMMING, A CUSCHIERI, RAB WOOD, KG WORMSLEY ................................................................... 1240Morphological study of the relation between accidental hypothermia and acute pancreatitis AK FOUUS ..... ................ 1244Liver function in septic shock JG BANKS, AK FOULIS, I MCA LEDINGHAM, RNM MaCSWEEN .................................... 1249Use of monoclonal antibodies for the histopathological diagnosis of human malignancy KC GATrER, Z ABDULAZIZ, P BEVERLEY, JRFCORVALAN, C FORD, EB LANE, M MOTA, JRG NASH, K PULFORD, H STEIN, J TAYLOR-PAPADIMITOU, C WOODHOUSE, DY MASON .... ..... 1253Measurement of striated muscle fibre diameters using interactive computer-aided microscopy G SLAVIN, C SOWTER, P WARD, KPATON ....................1...................................................................................1268Jejunal crypt cell abnormalities associated with methotrexate treatment in children with acute lymphoblastic leukaemia CRPINKERTON, CHS CAMERON, JM SLOAN, JFT GLASGOW, NIT GWEVAVA ........................................................... 1272Demonstration of calcification fronts by in vivo and in vitro tetracycline labelling i MCCLURE .............................. 1278Localisation of aluminium by histochemical and electron probe x-ray microanalytical techniques in bone tissue of cases of renalosteodystrophy PS SMITH, J MCCLURE ................................................................................ 1283Technical methodPost-mortem inflation and fixation of human lungs - a modification of available apparatus DCS DURRANT ..... .............. 1294

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Letters to the Editor .............................................................................................. 1295Book reviews ................................................................................................ 1298Some new titles ................................................................................................ 1300Notices ................................................................................................ 1300

No 12 DECEMBER 1982Page

A quantitative study of a-naphthyl acetate esterase-positive cells in Hodgkin's disease J CROCKER, EL JONES, RC CURRAN ....... 1301Lymphoma cells in cerebrospinal fluid confirmed by chromosome analysis J PEARSON, EB ILGREN, Al SPIGGS ..... ............ 1307Polycythaemia rubra vera and hairy cell leukaemia in the same patient: studies on the spleen GJ MUFFI, Ti HAMNUN, FK STVENSON,M FITCHETr ....................................................................................................... 1312Inhibitory effects of peripheral blood cells on in vitro colony formation by autologous bone marrow in aplastic anaemia: relationwith response to immunosuppressive therapy A BLASETTI, A FAILLE. N BALITRAND, E GLUCKMAN, A DEVERGIE, C DRESCH ......... 1316ICSH recommendations for the analysis of red cell, white cell and platelet size distribution curves: I General principles nmENGLAND, RM ROWAN, WH COULTER, W GRONER, JC KOEDAM, JA KOEPKE, SM LEWIS, A RICHARDSON JONES, R THOM, OW VaD ASSENDEF . 1320Comparison of platelet storage in PL 146 and PL 732'plastic packs: preliminary in vitro studies DR EDwAR CC ENTwi .L. 1323Measurement of body fluid proteins by polyacrylamide gel electrophoresis MH JOHNSON, EJ THOMPSON ..... ................. 1328Neural-tube defect risk assessment for individual pregnancies using alphafetoprotein and acetylcholinesterase test results rwSTRIKE, J SMITH .................................................................................................... 1334Is post partum rubella vaccination worthwhile? PD GRIFFITHS, C BABOONIAN .............................................. 1340Comparison of enzyme-linked immunosorbent assay, radioimmunoassay, complement fixation, anticomplement immunodluores-cence and passive haemagglutination techniques for detecting cytomegalovirus IgG antibody JC BooTH, GiLuLAN HANNINOTON, TMFBAKIR, H STERN, H KANGRO, PD GRIFFITHS, RB HEATH ........ ............................................................. 1345Modification of the system of screening for antisyphilis antibodies in a blood transfusion centre, featuring a miniaturisation of theTreponema pallidwn haemagglutination assay A PUCKETr, G PRATT ...................................................... 1349A simple disc technique for the presumptive identification of Legionella pneumophila MG smllH............................ 1353Velvet pad surface sampling of anaerobic and aerobic bacteria: an in vitro laboratory model D RAAHAVE, AIUCE FUES-MOLLER. . 1356The potential of bacteriocin typing in the study of Clostridium perfringens food poisoning GN WATSON, MF STINGER, R GILBERT, DEMAHONY..... 1361A concise biotyping system for differentiating strains of Escherichia coli R GARGAN, W BRUMFITr, JMT HAMILTON-MILLER ........ 1366Alveolar-cell carcinoma: a problem in sputum cytodiagnosis Al SPRIGGS, MARGARET COLE, MS DUNNILL....................... 1370Fatty tumours of the uterus DJ POUNDER ............................................................................ 1380Preparation of Epon-embedded renal tissue for scanning electron microscopy WL NG, L MA, KF SO ..... .................... 1384Lack of cross-reactivity between antibody against smooth muscle and antibodies to measles virus in sera from patients with chronicactive hepatitis Al SOMMER, G HAUKENES ........................................ 1388Technical methodInfluence of decalcification on immunohistochemical staining of formalin fixed paraffin-embedded tissue i MATrHEws ........ 1392Letters to the Editor .............................................................................................. 1394Book Reviews ............................................................................................... 1396

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