Molekulární biologie (KBC/MBIOG) Ivo Frébort Alberts et al. (2008) Molecular Biology of the Cell, 5th ed. Garland Science, New York 4. DNA Transcription and Translation
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Molekulární biologie(KBC/MBIOG)
Ivo FrébortAlberts et al. (2008) Molecular Biology of the Cell, 5th ed.
Garland Science, New York
4. DNA Transcription and Translation
Central dogma of molecular biology
Genes can be expressed with different efficiencies
Chemical structure of RNA
RNA folding
Structure of bacterial RNA polymerase
The transcription cycle of bacterial RNA polymerase
Transcription by RNA polymerase
Promoter and terminator in prokaryotes
Direction of transcription is given by the orientation of promoter sequence
(RNA synthesis always from 5‘ to 3‘)
RNA Pol IIBlue – MgRed - Zn
Initiation of transcription in eucaryotes
Transcription factors
Transcription complex in eucaryotes
From gene to protein in eucaryotes and procaryotes
Procaryotic and eucaryotic mRNA structures
Pre-mRNA processing in eucaryotes
Introns and exons
RNA splicing
Alternative splicing
The spliceosome reaction
The spliceosome reaction
Three types of RNA splicing
Rare self splicing intrones – evolutionary basis for spliceosomes
Length of introns and exons
Degradation of incorrectly spliced mRNA
Cleavage site of the terminator sequence and polyadenylation
Cleavage site of the terminator sequence and polyadenylation