Kefir administration reduced Kefir administration reduced progression of renal injury in STZprogression of renal injury in STZ--
diabetic ratsdiabetic rats
Cristina Stewart Bogsan
Pharmaceutical-Biochemical Technology Department
OutlineOutline
• Diabetic Mellitus;
• Kefir;
• Oxidative stress;
• Aim;• Aim;
• Protocol;
• Results;
• Conclusion.
Diabetes MellitusDiabetes MellitusDiabetes Mellitus (DM) is a
group of metabolic disorders thathave in common the hyperglycemia.
DM has become a seriouspublic health problem that affectspublic health problem that affectsmillions of individuals worldwide(Shaw, 2010).
30% of patients developnephropathy, the main cause ofmorbidity and mortality in diabeticpatients (Molitch et al., 2004).
Source: Shaw, 2010
HYPERGLYCEMIA
Increased ROS production
Impaired NO bioavailability
NF-kB Activation
Oxidative Stress in DMOxidative Stress in DM
bioavailability
Lipid peroxidation (LPO)
Endothelial dysfunction
Malandialdehyde (MPA)
Thiobarbituric acid substances (TBARS)
NF-kB Activation
Pro-inflamatorybiomarkers
Increase C-reactive protein (CRP)
KefirKefir• Kefir is a fermented milk that contains a complex
symbiotic mixture of Lactic Acid Bacteria (LAB) and
Molds.
• The main microorganisms are:• The main microorganisms are:o Lactobacillus,
o Lactococcus,
o Leuconostoc,
o Streptococcus,
o Kluyveromyces,
o Saccharomyces,
o Torula.
Kefir propertiesKefir properties
Kefir is known for providing benefits to human
health through its anti-inflammatory, immune-
stimulatory and antioxidant properties.
AIMAIM
This study aimed at assessing the effects of Kefir on
oxidative stress in diabetic animals.
STZ - diabetic induction45 mg/kg iv
Skimmed Milk
Fermentation until pH 4.6
20mg/ 100mL Kefir DA Inoculation
Kefir preparation
Stiring(1min)
Distribution in 50 mL cups
Cooling in ice bath
Storing (4 °C)
Kefir fermented milk
ProtocolProtocolSTZ Kefir
5 days 24 hs
Sacrificed
24 hs 8 weeks
Kidney histology
CTLCTL CTLKCTLK DMDM DMKDMK
Kefir groups intake 1.8 mL/day by gavageWater to CTL and DM groups
Water and Chow intakeDiuresis, WeightFasting Blood toleranceCreatinine,Proteinuria, Tbars, NO
CTLCTL CTLKCTLK DMDM DMKDMK
Water and Chow intakeDiuresis, WeightFasting Blood toleranceCreatinine,Proteinuria, Tbars, NO
Metabolic profile, renal function, and Metabolic profile, renal function, and oxidative stressoxidative stress
Metabolic profile, renal function, and Metabolic profile, renal function, and oxidative stressoxidative stress
GlycemiaGlycemia levelslevels
Oral Glucose Tolerance TestOral Glucose Tolerance Test
Renal functionRenal function
Renal functionRenal function
Oxidative StressOxidative Stress
Oxidative StressOxidative Stress
Inflammatory biomarkerInflammatory biomarker
Kidney histologyKidney histology
ConclusionConclusion
The results obtained in this study show that Kefir
treatment significantly reduced the progression of STZ-
induced hyperglycemia and oxidative stress in rats.induced hyperglycemia and oxidative stress in rats.
Kefir may play a role in slowing the metabolicchanges that contribute to DM as a non-pharmacological adjuvant .
AcknowledgmentsAcknowledgments
UnifespElisa M.S. Higaa,
Giovana R. Punaroa,
Fabiane R. Maciela,
Adelson M. Rodriguesa,
Silvia S.M. Iharab,
This work was supported by:
Coordenação de
Aperfeicoamento de Pessoal
de Nivel Superior (CAPES),
Silvia S.M. Iharab,
Sergio R.R. Araujob
USPMarcelo M. Rogeroc,
Marice N. Oliveirad,
Talita R.C. Sanchese,
Lucia C. Andradee,,
Fundação de Apoio a
Universidade Federal de São
Paulo (FAP-Unifesp),
Fundação de Amparo a
Pesquisa do Estado de São
Paulo (FAPESP).
Thank youThank [email protected]
