Kick Off!Small interfering RNA 12-12:03 • http://www.youtube.com/watch?v=btxqhK_ql1o

Central dogma 12:03-12:06• http://www.youtube.com/watch?v=9kOGOY7vthk

Nature video 12:06-12:11• http://www.youtube.com/watch?v=cK-OGB1_ELE

Rachel 12:11-12:15• We Talk Science introPraveena 12:15-35Discussion 12:35-50

Delaying Flowering in Alfalfa Using RNAi Technology

In

“We Talk Science”Seminar Series Spring 2014

By:Praveena Kanchupati

Graduate studentBio/Micro Department

SDSU

Plant Stress Physiology Lab

Dr. Yajun Wu Md. Rokebul Anower

Delaying flowering will prolong the vegetative growth: thus more ‘biomass’

http://nrcca.cals.cornell.edu/crop/CA2/CA020708.php

http://www.uni-konstanz.de/FuF/chemie/jhartig/

Gateway Cloning System

Schematic representation of Gateway cloning system

Gateway Entry vector

Hairpin RNA expression vector for RNAi

Gateway Destination vector

• pENTR™ Directional TOPO® Cloning Kits• Gateway® LR Clonase® II Enzyme mix

Invitrogen by Life Technologies

Copy/paste this link: http://gateway.psb.ugent.be/search

• Dr. Yajun Wu• Rokebul Anower• Committee members• USDA• NC Region SunGrant• Plant Science Department, SDSU

To contact me:

In person: Lab No: 130 , or, Desk No: 32 (Grad Area) Northern Plains Biostress SDSU

Shoot an email: praveena.kanchupati@sdstate.edu

On Facebook

NCBI Blast: http://blast.ncbi.nlm.nih.gov/Blast.cgi

Discussion