KOMP and KOMP2
Trish LaboskyScientific Co-director
International Project
KOMPKnock Out Mouse Project
Trans-NIH initiative, aims to generate a comprehensive and public resource of murine ESCs containing a null mutation in every gene in the mouse genome.
KOMP Goals1) Use gene targeting to generate null alleles,
marked with a high utility reporter, preferably in C57BL/6;
2) Develop improved C57BL/6 ES cells capable of robust germline transmission, to be used in a high throughput pipeline in generating this resource;
3) Support a repository to house the products of this resource, additional ‘repatriation' effort to bring in 1000 of the existing high priority mouse knockouts;
4) Implement a data coordination center to make the status and relevant data of the production effort available to the research community.
1. Regeneron Pharmaceuticals2. Children's Hospital Oakland Research Institute (CHORI), School of Veterinary Medicine, University of California, Davis (UC Davis) and the Wellcome Trust Sanger Institute.3. The Jackson Laboratory.
www.knockoutmouse.org
Additional KOMP miceNIH awarded contracts that gave academic researchers access to a set of well-characterized knockout mouse lines from Deltagen and Lexicon Genetics.
~ $11 million for ~250 lines of these mice
http://www.nih.gov/science/models/mouse/deltagenlexicon/list.html
http://www.nih.gov/science/models/mouse/deltagenlexicon/list.html
Nature (2011) 474, 262.
Vast majority of mice are from the Wellcome Trust
Have targeted more than 9000 genes.
Null allele
Null allele with reporter
Conditional allele
What if you don’t know which genes are involved in your biological system/question?
KOMP2: Newly Funded
HarwellJaxBaylorUC Davis
Phenotyping Platform
FeesMRRCC
KOMP Davis
What to do when you find a line of interest:
You submit MTA, order ESCs (we recommend the “PREMIUM ESC PACKAGE”, $2801), and have them sent to us.
Additional expenses:
ESC expansion, freezeback, prep for path screening $1000 / cloneESC Karyotyping (if needed)
$350 / clonePath screening by DAC
$350 / cloneESC prep / injection day
$450Injection into Albino BL6 / day
$2600Injection into BL6 / day
$1650
TMESCSR Website
http://www.vanderbiltresearch.org/community/profile/id/8