Lab 22 –Female Reproductive System II Introduction Female ... Repro_2.pdf · Female Reproductive...

FemaleReproductiveSystemII

Lab22– FemaleReproductiveSystemIIA560– Fall2015

I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs

A. GenitalTract1. Oviduct(Fallopiantube)2. Uterusa. Generalstructureb. Phasesi. Proliferativephaseii. Secretoryphaseiii. Onsetofmenstruation

c. Cervix3. Vagina

B. MammaryGlandIV. Summary

Learning Objectives

1. Identify the layers in the wall of the uterine tube, know the functions ofthe component cells, and recognize the different tubal regions.

2. Recognize the layers of the uterine wall, the functional significance oftheir components, and their changes during the menstrual cycle.

3. Recognize histological feature of the cervix, ectocervix, and vagina.

4. Understand the structure of the mammary gland and how it changesduring puberty, pregnancy, and lactation.




c. Cervix3. Vagina


Keywords

basal layercervixectocervixendometrial glandsendometriumfibrous support tissuefunctional layer

lactiferous ductmammary glandmyoepithelial cellsmyometriumperimetriumuterusvagina




c. Cervix3. Vagina





c. Cervix3. Vagina


Slide60:Oviduct,Ampulla

Slide59:Oviduct,Isthmus

Ovary




c. Cervix3. Vagina


Slide19:Oviduct,MassonTrichrome

serosa

mucosa

muscularis




c. Cervix3. Vagina



mucosasimplecolumnarepitheliumandthinlaminapropria

muscularis

serosa




c. Cervix3. Vagina



ciliatedcellsciliahelpsweep

fluidtowarduterus

secretorypegcellsnon‐ciliated;secrete

nutritivefilm

laminapropria




c. Cervix3. Vagina


Slide60:Oviduct,Ampulla

Slide59:Oviduct,Isthmus

Examineslides59and60oftheoviducttoidentifythefollowingstructure:

mucosa muscularis serosa

Howdothestructurescomparefromoneslidetotheother?




c. Cervix3. Vagina


Slide1:Uterus,Trichrome

endometrium

myometrium

perimetrium




c. Cervix3. Vagina


endometriummucosawithsimplecolumnarepitheliumandthickunderlyinglaminapropria containingglands

endometrial(uterine)glands

myometrium





c. Cervix3. Vagina



perimetriumprimarilyserosa

myometriumendometrium




c. Cervix3. Vagina


functionallayer

basallayer

LayersofEndometrium

myometrium




c. Cervix3. Vagina


MenstrualCycle

1)MenstrualPhaseSlide81

2)ProliferativePhaseSlide80Slide83

3)SecretoryPhaseSlide82Slide84




c. Cervix3. Vagina


Slide80:Endometrium,H&E

myometrium

perimetrium

endometrium




c. Cervix3. Vagina



myometrium

• fewglandsinendometrium• lotsofspacebetweenglands

endometrialglands




c. Cervix3. Vagina


endometrium

myometrium





c. Cervix3. Vagina



• morenumerous,jaggedglands

• presenceofthin‐walled,blood‐filledlacunaeinstroma

vascularlacunae

endometrialglands



A. GenitalTract1. Oviduct(Fallopiantube)2. Uterusa. Generalstructureb. Phasesi. Proliferativephaseii. Secretoryphaseiii.Onsetofmenstruation

c. Cervix3. Vagina


myometriumendometrium




A. GenitalTract1. Oviduct(Fallopiantube)2. Uterusa. Generalstructureb. Phasesi. Proliferativephaseii. Secretoryphaseiii.Onsetofmenstruation

c. Cervix3. Vagina



• bloodinstroma,notinlacunae

• thick,well‐developedfunctionallayer

bloodinstroma




c. Cervix3. Vagina


Slide133:Cervix,H&E

cutedgestratifiedsquamousepithelium

cervicalglands

transitionofepithelium




c. Cervix3. Vagina


Slide133:Cervix,H&E

endocervical gland

ectocervix




c. Cervix3. Vagina


Slide108:MammaryGland,H&E

secretoryacini(lobule)

lobuleconsistsofmultipleacinialldrainingtosame

terminalduct

fibrousinterlobularsupporttissue

adipose




c. Cervix3. Vagina



lactiferousduct

secretoryacini(lobules)

fibroussupporttissue




c. Cervix3. Vagina



plasmacells

myoepithelialcell insidebasementmembranesecretoryaciniandductsarebothcomposedoftwolayersofcells:theluminalepithelialcellsandthebasalmyoepithelialcells




c. Cervix3. Vagina


Common Confusion:Seminal vesicle vs. Ampulla (of Oviduct)

Seminal Vesicle

Seminal vesicle: secretory gland of male reproductivetract, producing majority of seminal fluid volume; consistsof single tube coiled upon itself; joins with vas deferens toform ejaculatory duct

Look for: (1) glandular with the appearance of multiplelumens as result of coiling of tube; (2) highly‐foldedmucosa, often having fine “honeycomb” appearance; (3)non‐ciliated pseudostratified columnar epithelium withlipid droplets in cytoplasm giving “foamy” appearance; (4)lipofuscin pigment is often seen in mucosa

Ampulla of oviduct (uterine tube, Fallopian tube, orsalpinx): tube leading from ovary to uterus; tube transmitsovum to uterus and is the usual site of fertilization, usuallyin the ampulla

Look for: (1) single lumen tube w/ mucosa surrounded bymuscularis and serosa continuous with broad ligament; (2)mucosal folds often appear “thicker” than in seminalvesicle due to more lamina propria in the folds; (3) ciliatedsimple columnar epithelium and non‐ciliated “peg cells”present in mucosa; (4) size of muscularis changes withregion of oviduct, generally thin in ampulla and thick inisthmusOviduct

Time (Days) of Cycle Influencing Hormones Thickness of Endometrium Endometrial Glands Vasculature

Proliferativephase

Secretory phase

Menstrualphase

Lab22‐ Characteristicsofuterinewallthroughoutmenstrualcycle

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