Departmentof PhysiologyUniversity of Kansas Medical Center

Annual ReportJuly 1, 1968 to June 30, 1969

ACADEMIC STAFFE. B. Brown, Jr., Ph. D., Professor and ChairmanAlan M. Thompson, Ph. D., ProfessorDonald Johnson, Ph. D., Associate ProfessorG. N. Loofbourrow, Ph. D., Associate ProfessorL. P. Sullivan, Ph. D., Associate ProfessorKenneth L. Goetz, Ph. D., Assistant ProfessorJames Rhodes, M. D., Assistant ProfessorJohn W. Trank, Ph. D., Assistant ProfessorA. Stark Wolkoff, M. D. Assistant ProfessorMerrill Tarr, Ph. D., Assistant Professor

POSTDOCTORAL FELLOWSPaul Hensleigh, M. D., supported by Ob-Gyn Training Grant.James Scherbenske, Ph. D., P. H. S. Postdoctoral Fellow, 9-1-67 to 8-31-68.Arlo Hermreck, M. D. , Postdoctoral Research Fellow, P. H. S. 7 -1-67 to 6 -30-69.

GRADUA TE STUDENTS

ILarry Welling, M. D.Paul Hensleigh, M. D.Conrad Johanson./

*Barbara McDougal v'# Martin Kramen I# Dennis Waring .t ..-

Gary Bond .-

Sam Steele, M. A. , M. D.\

Carol Hend~ey'/t Gary Slick, D. O.#-Arlo Hermreck, M. D.

Lester Wade"Cleve Howa'rd, M. D.

.Edward Martin.Frank ScammanKyra Riegle -Yih Loo ng LaiChung-Ning RangRichard Williams

DegreeSought

Ph. D.Ph. D.Ph. D.Ph. D.Ph. D.Ph. D.Ph. D.Ph. D.Ph. D.M. A.Ph. D.Ph. D.M.A.Ph. D.Ph. D.Ph. D.Ph. D.Ph.D.M.A.

* Ph. D. granted June 1969.# Will complete work for Ph. D. August 1969.t Work for M. A. completed June 1969.

Preliminary ExaminationsWritten Oral

Aprill968 May 1968May 1969 June 1969May 1968 May 1968March 1968 March 1968March 1968 March 1968May 1968 May 1968May 1968 May 1968Military Servo 6-6-68. .March 1969 April 1969Final Exam May 1969Dec. 1968 Dec~ 1968

USAF

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KAW VALLEY HEART STUDENTS (Science Talent Search Program-summer 1968. )

Mic hae1 F oggsCarter Hill

Debra WynnSusan VandergriffWilliam WilsonMike TrenbeathJoan Jarvis

SUMMER RESEARCH FELLOWS (Medical Students)

Mike McGuireMike CannCharles PaxsonLinda Steel

TEACHING

The Physiology for Medical Students course was again scheduled duringthe winter and spring quarter s with the last two weeks of the year devotedto the combined course in Endocrinology.

GRADUATE COURSES

#325 Biology of Sex, Dr. Johnson - FaU1968#327 Physiology Instrumentation - Dr. Trank - Winter 1968#320 Acid-Base Balance and Electrolyte Metabolism - Dr. Brown - Spring 1969

GRADUATE SEMINARS #351

Respiratory Control - Dr. Brown - Winter quarter 1968-69.

OUTSIDE SPEAKERS AND VISITORS

July 9, 1968 Dr. Ivor H. Mills, Chairman of Medicine Department.Cambridge University. "Clinical Aspects of AdrenalFunction." Dr. Mills is noted for his work on endocrinecontrol of renal function and spoke on some aspect ofthis subject.

July 8, 1968 Dr. Jared Grantham, Lab. of Kidney and ElectrolyteMetabolism, National Heart Institute, Bethesda, Md.Joint Medicine-Physiology Seminar on "Mechanism ofAction of Anti-diuretic hormone in isolated renal

collecting tubules. "

3(Outside Speakersand Visitors - continued)

Sept.. 3, 1968 Dr. Charles Michel, Physiology Laboratories,Oxford, England. I'Diffusion of Ions out of SingleCapillaries and acros s Mesothelium. "

Sept. 24, 1968

Oct. 7, 1968

Oct. 29, 1968

Nov. 18, 1968

Dec. 9, 1968

Feb. 1969

March 17, 1969

March 24, 1969

April 7, 1969

Dr. Alexander Leaf, Chief of Medical Services,Massachusetts General Hospital. "The Action ofAldosterone. "

Dr. G. W. Kreutzberg, Max-Planck-Institut fuerPsychiatrie, Munich. "Experimental Degenerationand Regeneration in Motor Neurons and PhysiologicalImplications. II

Dr. Ernst Gellhorn, Emeritus Professor of Neuro-

physiology, Univ. of Minnesota. "Levels ofHomeostatic Adjustment Reactions. "

rDr. Jean Crabbe, Assoc. Pro£. of Medicine and

Physiology, Univ. of Lauvain, Lauvain, Belgium.I'Hormonal Stimulation of Sodium Transport. "

Dr. Arthur B. Lee, Dept. of Surgery, College ofPhysicians and Surgeons of Columbia University,New York, N. Y. "The Effect of Abdominal Surgery

Upon the Control of Breathing. "

Dr. Dennis Dahl, Res. Assoc., K. U. at Lawrence,

"Short Chain Fatty Acid Inhibition of Rat Brain Na-KATPase. "

Dr. J. M. Yochim, Assoc. Prof. of Physiol. andCell Biology, KU at Lawrence, "Metabolism of theUterus and its Relationship to Implantation. 11

Dr. James O. Davis, Pro£. and Chairman of Physiology,

University of Missouri School of Medicine, Columbia,Missouri, "Humoral Factors in the Regulation ofRenal Sodium Excretion. "

Dr. Nancy Dahl, Asst. Pro£. Dept. of Physiology andCell Biology, University of Kansas, Lawrence,"Depression of nerve electrical activity by puromycininvolving a mechanism other than inhibited protein

synthesis. II

4(Outside Speakers and Visitors - continued)

April 21, 1969 Dr. Francis J. Haddy, Professor and Chairman,Physiology Department, Michigan State University."Osmolarity and Vascular Resistance. "

April 28, 1969 Dr. Wm. M. Bass, Prof., Physical Anthropology,K. U. at Lawrence. "Your Bones are your 1. D. "

May 12, 1969 Dr. Wm. G. Kubicek, Prof. Dept. Physical Medicineand Rehabilitation, Univ. of Minnesota, MedicalSchool, Minneapolis, Minnesota. "ImpedanceCardiography. 11

TEACHING

The Physiology course for medical student s was taught along the usuallines, however some thought was given to new approaches and some experi-mentation was carried out in presenting the practical portion of the course,looking forward to the pos sible requirements of a new curriculum. Wesubstituted demonstrations for several difficult experiments which havebeen carried out by students in previous years. It appeared that thisprocedure was quite successful, as judged by faculty and student response,but of course these evaluations as always are subjective. We plan tocontinue experimenting with some of these ideas during the next academicyear.

During the past year we added two new courses to our offerings forgraduate students: a course on Gastroenterology by Dr. Rhodes, and acourse on Excitable Membranes by Dr. Tarr. Both of these courseshave been approved by the Graduate Council.

STAFF

Dr. Alan Thompson was away from the department on a sabbatical leavein Copenhagen from January until June. His duties were shared by severalmembers of the department in his absence. On January 1, Dr. Goetz beganan internship at St. Luke's Hospital and his appointment was changed fromAssistant Professor to Lecturer. Dr. Richard L. Clancy joined our staffas Assistant Professor on July 1, 1969. He brings added strength to ourdepartment in the area of cardiovascular physiology.

GRADUA TE STUDENTS

We admitted two new graduate students at the beginning of the academicyear; Mr. Yih Loong Lai and Mr. Chung-ning Pang. Both of these studentshave demonstrated their ability to stand well into the upper part of the classin competition with our medical students, and we expect both of them to

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proceed toward the Ph. D. degrees. The calendar year 1969 will be themost productive for Ph. D's in Physiology produced by this departmentsince our move to this campus. Barbara McDougal received the Ph. D.at the June commencement exercises, Martin Kramen and Dennis

Waring are completing their thesis work this month, and Dr. ArloHermreck expects to complete his work for the Ph. D. before the endof this calendar year.

AC TIVITIES OF STAFF

Dr. E. B. Brown, Jr., Professor and Chairman.

Dr. Brown was on sabbatical leave working in the Physiology laboratories atOxford University, England, with Drs. Cunningham, Lloyd, and Michel, from9/1/67 through 8/31/68.

June 1968 - presented a paper on "Effects of Breathing through a Tube" beforeBritish Physiol. Soc., Oxford, England.

July 1968 - Attended meeting of British Physiological Soc., Edinburgh, Scotland.

August 1968 - Presented invited paper on "Whole Body CO2 Exchange" beforeXXIV International Physiological Congress, Washington, D. C.

October 1968 - Attended Workshop on "Role of the University in Graduate MedicalEducation" and chaired a discussion group. Workshop was sponsored byCouncil of Academic Societies of AMA and held in Washington, D. C.

October 1968 - Presented invited paper on "Fundamentals of Acid-Base Balance"before American Society of Anesthesiologists, Washington, D. C.

October 1968 - Accompanied Drs. Scarpelli, Cuppage, and Holder on two-dayvisit to Case Western Reserve Medical School to learn about their curriculum.

Dec. 1968 - Gave seminar in Physiology Department on Lawrence Campus.

Jan. 1969 - Presented eight lectures for medical students at Univ. of Calif. ,California Medical College, Los Angeles.

Jan. 1969 - Board Member of the Kansas Heart Association.

Feb. 1969 - Serving on Great Plains Area Heart Assn. Res. Comm. meetingin Omaha, Nebraska. Elected Vice-Chairman of the Comrn at this meeting.

9/1/67-3/31/69 - Secretary-Treasurer, Association of Chairmen ofDepartments of Physiology.

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Dr. Alan M. Thompson, ProfessorTwo Kaw Valley Fellows, Carter Hill and Deborah Wynn, and a medical

student, Steve Brunner, worked in my laboratory during the summer of 1968.

Attended International Physiological Congress in Washington, D. C. in Aug. 1968.

Interim Secretary-Treasurer of Association of Chairmen of Departments ofPhysiology until Dr. Brown's return from sabbatical.

On Sabbatical Jan. 1 to May 31, 1969, working with Dr. Christian Crone onbrain permeability studies at the University of Copenhagen.

In Copenhagen, Denmark, presented work at a joiat Iastitute seminar and atthe spring meetiag of the Scaadinavian Physiological Society.

Preseated work on May 10 at the annual coafereace on Transport at theUniversity of Aarhus, Denmark.

Presented work at aa invited symposium 00 Capillary Transport (to be

published by Munksgaard) on Juae 22-26, 1969, ia Copenhagen.

Graduate advisor for Conrad Johanson and Lester Wade.

Dr. Kenneth L. Goetz, As sistant Professor

Dr. Goetz atteaded the Annual Meetings of the Am. Heart Association iaBal Harbour, Florida, November 17-24, 1968.

FASEB meetings, Atlaatic City, New Jersey, April 1969.

Began medical internship at St. Luke's Hospital, K. C., Missouri, January, 1969.

Received teaching award from Medical School Class of 1971.

Presented eight series of lectures on cardiovascular physiology to coronarycare nursing class of Regional Medical Programs.

Acted as advisor for Arlo S. Hermreck, M. D., Postdoctoral Fellow.

Served as consultant to Midwest Research Institute.

Gave series of lectures ia Physiology to staff, St. Luke's Hospital.

Supervised work of Gary Slick for M. S. in Physiology.completed ia June, 1969.

Requiremeats

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Dr. Donald C. Johnson, Associate Professor

Society for the study of Reproduction: Nashville, Tennessee, Aug. 28-30,1968 attended with Martin Kramen, R. H. Naqvi, Carol Hendley.

American Association Advancement of Science. Dallas, Texas, Dec. 26-31,

Presented paper: Effect of Cyproterone acetate on pituitary LH inimmature rats. This was with the American Society of Zoologist, Div. ofComparative Endocrinology.

1968.

Federation Meetings: Atlantic City, April 1969.Presented paper: Cyclic changes in FSH induced by a single injectionof testosterone propionate.

Dr. G. N. Loofbourrow, Associate Professor

Writing Neurophysiology Textbook.

Consultant, VA Hospital, Kansas City, Missouri

Participated in postgraduate symposium on The Sociobiology of Aging at KUMCNov. 1968. Gave lecture on the Physiology of Aging.

April 1969 attended 11th Annual Meeting of Council on Medical Television, inK. C.

Recruitment trip for Graduate School candidates to St. Benedicts College,Atchison, Kansas.

In charge of Department Seminar 1968-69.

Department Librarian 1968-69.

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Dr. James B. Rhodes, Assistant Professor

American College of Physicians meeting, Kansas branch, November 1968.

Midwest Gut Club, Rochester, Minnesota, March 1969.

American Federation of the Societies of Experimental Biology, Atlantic City,New Jersey, Spring 1969.

American Gastroentero1ogica1 Association and Endoscopy Association, Washington,D. C., May 1969.

Addressed the St. Joseph Academy of General Practice in Association withDoctor Rising's Postgraduate Program in September 1968.

Participated in the postgraduate program with a talk on Peptic Acid Distress inNovember 1968.

Grand Rounds on Carbohydrate and Protein Digestion and Absorption in October,1968.

Lecture on Cell Pharmacology of the GI Tract for the Department of Pharmacologygraduate students inFebruary 1969.

Addressed the graduate students at Southwest Missouri State College inSpringfield on current concepts of carbohydrate digestion at a biology seminarin March 1969.

Seminar, Department of Physiology, on current status of protein digestion,May 1969.

Elected treasurer of the local Society of Sigma Xi this spring.

Member of the Tempora:ry Faculty Planning Committee during the year andcurrent member of the steering committee of the permanent medical facultyorganization.

Throughout the school year, a Saturday elective program and correlated gastro-enterology is offered. This meets weekly for an hour on Saturday mornings.

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Dr. Lawrence P. Sullivan, Associate Professor

Sept. 1968 - International Physiological Congress.

American Society of Nephrology - Nov. 1968.

April 1969 - Fed. Am. Soc. Exper. BioI.

IV International Congress of Nephrology - June 1969.

Presented two lectures to House Staff at St. Luke's Hospital K. C., Mo.

Local Committees:

Research

Curriculum

Renal Dialysis and Transplant

Graduate advisor for Barbara McDougal who completed Ph. D. requirements

in Spring of 1969, and Dennis Waring who will complete Ph. D. requirementsin fall of 1969.

Lectures on Diuretics in Medical Pharmacology Course.

Dr. Merrill Tarr, Assistant Professor

Attended the XXIV International Physiological Congress, Washington, D. C.

in August 1968.

Attended Spring Meetings of the APS in Atlantic City, New Jersey, in

Aprill969.

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Dr. John W. Trank, Assistant Professor

Lectured on .'Biomedical Engineering--present and future" at Midwest ResearchInstitute in April 1969.

Consultant in Biomedical Engineering to Midwest Research Institute.

Served on Council for Human Response to Vibration.

Attended meeting of Task Force on Human Response to Vibration.October 14, 1968, in New York.

Presented a series of lectures on medical electronics instrumentation toE. E. 128 course at Lawrence.

Served as expert witness and consultant on Electrical Interference with medicalinstrumentation for Baptist Memorial Hospital.

Recruitment trip to Springfield, Missouri, Pittsburg, Kansas, forGraduate Student Procurement.

Supervised departmental shop maintenance and design function.

Presented invited lecture on careers in Physiology and Bioengineering atSt. Anns School, May 1969.

Presented preliminary proposal for KUMC Bioengineering program to themedical center administration.

Acted as academic advisor to Mr. Gary Bond.

Dr. A. Stark Wolkoff, Assistant Professor

Attended APS meeting.

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RESEARCH GRANTS ACTIVE DURING THE YEAR

1ROl HE 12231 Intracellular Compensation for Chronic Pca Changes.-01 year (AFy) (1722) from 10-1-68 to 9-30-69. $24,063.' 2PHS Heart Institute Grant.

Kaw Valley Heart #9725 from 1-1-69 to 12-31-69 for $2,700,"Respiratory Control." Dr. E. B. Brown, Jr.

#AF41(609)-3005, USAF Sch. Aerospace Med., Brooks Air Force Base, Texas. qL-<

"Intracellular compensatory changes in response to chronic respi~atory (/:6-acidosis and alkalosis.!! Extended to Dec. 31, 1968, without additional funds. '

I 'iZ'(, C (

PHS Training Grant. Program Director: E. B. Brown, Jr. (1226)#5T1GM1l37 -05 from July 1, 1968 to June 30, 1969, $33,334.

NSF #GB-4314 - Dr. L. P. Sullivan. Feb. 1, 1967 to May 31, 1968 (extendedto 8- 31-69) on "Factors controlling renal excretion of ammonium, hydrogenand potassium. "

#5-K3-GM, 11,898-04 from 7-1-68 to 6-30-69, $19,342 Career DevelopmentAward - "Renal tubular potassium transport." Dr. L. P. Sullivan.

#1 ROl AM 10227, USPHS from 1-1-68 to Dec. 31, 1968, $13,949. 1-1-69 to12-31-69 $21,401 - 2 R01-AM 10227 -04, "Diuretic agents and ion transportin the toad bladder." Dr. L. P. Sullivan.

Kansas Heart Association $2,500, July 1, 1968 to June 30, 1969, to Dr. K. L.Goetz, principal investigator for "Coronary blood flow as related to rightheart pressure. "

USPHS #HE1l560-02, 6-1-69 to 5-31-70, $17,458. itA study of Atrial

Receptors. Dr. K. L. Goetz.

Alan M. Thompson - USPHS NB 07059-02, 1-1-68 to 12-31-68 $26,585.1-1-69 to 12-31-69 (03 year) $17,285. "Nonelectrolyte transport alterationin perfused brain. It

Alan M. Thompson - Co-Investigator on USPHS #NB HE 06907 -02,June 1, 1967 to August 31, 1968. $60,000. Interdepartmental project granton "Prognosis and Theory in Cerebrovascular Disease. 11

12

American Cancer Society - Jan. 1, 1968 to December 31, 1968, $9,900.

.'Role of Gonadotropins in Experimental Ovarian Tumorigenesis. "Dr. D. C. Johnson.

~

NIH #ROl-HD 03097 -01 - Dr. I:.C. Johnson - Follicle Stimulating Hormone ..

Control in the Male. March 1, 1968-Feb. 28, 1969, $15,352. r

NSF #GB 8067 - 9-1-68 to 8-31-70 $19,700.Patterns. Dr. D. C. Johnson.

"Hypophyseal Gonadotropin

Dr. C. M. Tarr, PHS #1 Rol HE 12426-012-1-69 to 1-31-72.

1st year from 2-1-69 to 1-31-70 - $14, 216, "Voltage Clamp of Cardiac Muscle. 11

68R1892, Ks. Heart, 7-1-68 to 6-30-69 for $2,750, "Voltage Clamp of CardiacMuscle.1I Dr. C. M. Tarr.

#9737, C. M. Tarr - Kaw Valley Heart Grant - Jan. 1, 1969 to Dec. 31, 1969.

James B. Rhodes, M. D.

#5 Rol AM 12329-02 PHS 6-1-68 to 5-31-71 - 3 year period $37,928.

6-1-68 to 5-31-69 1st year $15,540

"Intestinal peptidases and peptides hydrolysis. 11 (Arthritis / Metab. Dis. )

PUBLICATIONS:

Brown, E. B., Jr. and C. C. Michel. Whole body CO2 exchange. Proc.XXIV International Congress of Physiological Sciences 6:185, 1968.

Goode, R. C., E. B. Brown, Jr., M. G. Howson and D. J. C. Cunningham.

Tube breathing and ventilation. Proc. XXIV International Congress ofPhysiological Sciences 7:163, 1968.

Brown, E. B., Jr., D. J. C. Cunningham, R. C. Goode and M. G. Howson.

Respiratory effectsof breathing through a tube. J. Physiol., Vol. 198,

No.1, 1968.

Goode, R. D., E. B. Brown, Jr., M. G. Howson and D. J. C. Cunningham.

Respiratoryeffects of breathing down a tube. RespirationPhysiology6:343-359, 1969.

Bristow, J. D., E. B. Brown, Jr., D. J. C. Cunningham, R. C. Goode,

M. G. Howson, and P. Sleight. The influence of ventilation, carbondioxide and hypoxia on the baroreceptor reflex in man. J. Physiol.(in press).

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Bristow,J. D., E. B. Brown, Jr., D. J. C. Cunningham, R. C. Goode,M. G. Howson, T. G. Pickering and P. Sleight. Changes in the baro-receptor-cardiac reflex in exercise. J. Physiol. (in press).

Goetz, K. L., G. L. Slick, H. S. Starke and A. S. Hermreck., The effect ofatrial volume receptors on urine output in conscious dogs. (Abs.) Am.Soc. Nephrology. p 21, 1968.

Goetz, K. L., A. S. Hermreck, and J. W. Trank. Contribution of atrial re-

ceptors to diuresis following plasma expansion in conscious dogs. Fed.Proc. 28:584, 1969. (Abs.)

Pendleton, R. G., L. P. Sullivan, J. M. Tucker and R. G. Stephenson III.The effect of a benzothiaziadiazide on the isolated toad bladder. J. ofPharmacology and Experimental Therapeutics 164:348-361, 1968.

Pendleton, R. G., L. P. Sullivan and J. M. Tucker. The effect of chlormero-

drin on the isolated toad bladder. J. Pharmacol. and Exp. Therap.164:362-370, 1968.

Sullivan, L. P., R. G. Pendleton and J. M. Tucker. The effect of chlormero-

drin on the Toad Bladder. Am. Soc. Nephrology Abstracts, p. 66, 1968.

Scherbenske, M. J., L. P. Sullivan, and J. M. Tucker. The effect of Furo-samide on the toad bladder: A comparison with the effect of a thiazide.Am. Soc. of Nephrology p. 56, 1968.

McJ:ougal, B. and L. P. Sullivan. Studies of the Mechanism of Action ofBendroflumethiazide. Fed. Proc. 28:740, 1968.

Loofbourrow, G. N. "Grow old along with me: Physiological aspects of aging. "J. Kans. Med. Society 70(3) :109-996, March 1969.

G. D. Dunn, J. H. Fagan, and J. B. Rhodes. Gastric Photography, KansasMedical Society Journal, Vol. LXIX, No. 111, March 1968, pp. 138-141.

Rhodes, J. B. Peptidases of the small intestine, Handbook of Physiology,Chapter 121, pp. 2589-2604, 1968.

Moore, J. W., H. G. Haas, and M. Tarr. "Veratrine effect on sodiumconductance in squidaxon (Abstract). Proceedings of the InternationalUnion of Physiological Sciences. VIl:303, 1968.

Haas, H. G. and M. Tarr. "Membrane currents in frog atria as affected bymetabolic inhibition. (Abstract). Pflugers Archir. 307 :R31, 1969.

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Boyd, R. and r. C. Johnson, Gonadotropin patterns in male and female rats:

Inhibitionof LH release by testosterone propionate in animals gonadec-tomized atpuberty Acta Endocrinologica 58 :600-613. (1968).

Greenwald, G. S. and D. C. Johnson, Gonadotropic requirements for

maintenance of pregnancy in the hypophysectomized rat. Endocrinology 83,1052-1065 (1968).

Johnson, D. C., Elevation of plasma gonadotropins in unilaterally castrated

male rats. J. of Endocrinology 43 :311-312. (1969).

Johnson, D. C. and R. H. Naqvi, The effect of cyproterone acetate on LH in

immature rats. Endocrinology 84:421-426, 1969.

Johnson, D. C. and R. H. Naqvi, The effectof exogenous androgen on the

Steelman-Pohley assay for folliclestimulating hormone. Proc. Soc.

Exper. BioI. and Ivied. 130:1113-1116,1969.

Naqvi, R. H. and D. C. Johnson, Increased production of follicle stimulating

hormone in immature female rats by a single injection of androgen or

o'estrogen. J. Endocrinology 45:1-8, 1969.

Johnson, D. C. and R. H. Naqvi, A positive feedback action of androgen on

pituitaryfolliclestimulating hormone: Induction of a cyclic phenomenon.

Endocrinology, (inpress).

Thompson, A. lv1.and E. Hart. Effect of dimethyl sulfoxide on uptake of C14-

sucrose by perfused rat brain. Fed. Proc. 27:333, 1968.