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International CME on Renal Pathology, Department of Pathology , SGPGI, Lucknow, INDIA
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14th - 16th March, 2005Coordinator: R.K. Gupta
Professor & Head
Department of Pathology
SGPGIMS, Lucknow, India
Department of Pathology
Sanjay Gandhi Postgraduate
Institute of Medical Sciences
Lucknow- 226014, INDIA
US Coordinator: Surya V. Seshan
Professor of Pathology
Chief, Renal Pathology
Weill Medical College of Cornell University
New York, NY
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International CME on Renal Pathology, Department of Pathology , SGPGI, Lucknow, INDIA
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Go toCaseIndex Slide Seminar - IPathology of Glomerular Diseases
Surya V SeshanProf. of PathologyChief, Renal PathologyWeill Medical College of Cornell University New York, NY
Sharda Sabnis Chief,
Division of Renal Pathology
Armed Forces
Institute of Pathology
Washington DC
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International CME on Renal Pathology, Department of Pathology , SGPGI, Lucknow, INDIA
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Pathology of Glomerular Diseases Surya V Seshan & Sharda Sabnis
• Case 1: 32 year obese white male with proteinuria
• Case 2: 35 year female with proteinuruia & hematuria • Case 3: 48 year female with fever & proteinuria
• Case 4: 48 year female with fever, nephrotic syndrome & HTN
• Case 5: 55 yr male with H/O of HTN & atherosclerotic heart disease
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Case 1H/O• 32 year obese white male, a known case of
nephrotic syndrome for last 5 months, presented with – increased proteinuria (from 4.0 to 8.5 gm/day), – increased creatinine (from 1.1 to 3.1 mg/dL) – and decreased creatinine clearance (96 to 44 cc/min)
• No family history of renal diseaseP/E:
– BP 130/80 mm Hg– lower extremity edema
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Case 1Lab data:• U/A 3+ protein (8.5 gm/day), 4-5 RBCs/hpf &
fatty casts.– BUN 31.0 mg/dL– S. Cr 3.1 mg/dL– Bld. sugar 115 mg/dL– S. albumin 2.6 gm/dL– S. cholesterol 262 mg/dL
• ANCA, ASO, VDRL, FANA negative, complements normal
• Open biopsy performed due to obesity
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Slide Seminar- I: Pathology of Glomerular Diseases - Case 1
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Slide Seminar - I: Pathology of Glomerular Diseases - Case 1
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Case 1
? Diagnosis
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DISCUSSION International CME on Renal Pathology, Department of Pathology , SGPGI, Lucknow, INDIA
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Case 1
Differential diagnosis• Focal segmental glomerulosclerosis, idiopathic
or secondary (?obesity)• Membranous Glomerulopathy• Minimal Change Disease
Less likely• MPGN, Diabetic glomerulosclerosis, amyloid etc.
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Immunofluorescence microscopy • Five glomeruli• IgM and C3: 2+ to 3+ in mesangium in all glomeruli.• IgG, IgA, C1q, albumin, fibrinogen, and kappa and
lambda light chains negative.• These results suggest FSGS or IgM nephropathy or
diabetic glomerulosclerosis.
Additional information• Had a previous clinical diagnosis of Fabry disease and
was followed up.• Angiokeratoma skin lesions were noted on the back.• Neurological and cardiac exam not documented.
Case 1
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DISCUSSION International CME on Renal Pathology, Department of Pathology , SGPGI, Lucknow, INDIA
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Fabry Disease• End stage renal disease is common in hemizygous male around 3rd
to 5th decade. Hetertozygous females have an attenuated disease. • Renal accumulation of glycosphingolipids is seen mainly in
podycytes, tubular epithelium, followed by endothelium and mesangial cells and myointimal cells of BVs.
• Evident as membrane bound Zebra bodies with “onion skin” pattern or concentric lamellation with a periodicity of 35 to 50 A˚
• Glomeruli show mesangial widening thick BMs and focal or global sclerosis. Recurs in transplants but does not cause problems.
• Death around 5th decade due to severe cardiac or CNS complications.
• Recent studies of Rx with enzyme replacement infusion with purified alpha-Gal A produced by a genetically engineered human cell line or Chinese hamster ovocyte, seems to be effective and safe.
Case 1
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Final diagnosisRenal involvement in Fabry disease with
changes of focal segmental glomerulosclerosis
Extensive chronic tubulo-interstitial disease
Arterial and arteriolar sclerosis.
Case 1
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DISCUSSION International CME on Renal Pathology, Department of Pathology , SGPGI, Lucknow, INDIA
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References:1. Sessa A, Meroni M et al. Renal involvement in Anderson-
Fabry disease. Nephrol 2003 March-April 16(2):310-3
2. Schiffmann R, Kopp J et al. Enzyme replacement therapy in Fabry disease: a randomized controlled trial. JAMA. 2001 Jun 6; 285 (21): 2743-9.
3. Cho ME, Kopp JB. Fabry disease in the era of enzyme replacement therapy: a renal perspective. Pediatr Nephrol 2004 Jun 19(6):583-93
4. SessaA, Meroni M et al. Evolution of renal pathology in Fabry disease. Acta Pediatr suppl. 2003 Dec;92(443):6-8
Case 1
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Case 2H/O• 35 year Indian female• Abdominal discomfort 4-5 months.• Increased frequency , nocturia 4-5months.• Low grade fever - 3 months• No chills or rigors, no joint pain, rash, petechiae, hair loss.• No cough, neurological symptoms.• Found to have proteinuria & hematuria - July 03• Past h/o Jaundice 10 years back.• Obstetric history- NormalExamination• Pulse- 84/min, regular,BP-130/90 mmHg.• No edema, petechiae, or lymphadenopathy.• Systemic examination –NAD.
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Case 2Investigation: • Urine- Sp Gr-1025, 3+ Prot., WBC-30-35/HPF,
RBC-50-60/HPF• 24 hour urine protein 1.2gm/day• Hb 9.2gm, TC-3580 cells/mm3
• S.Creat-1.1mg/dl, T.Prot/Alb-6.4/3.0, T.Bilirubin-0.5/0.25 • SGOT/SGPT-32/20 IU/L• Anti-HCV Ab positive. HCV-RNA-Awaited• HBsAg- Negative.• C3 - 42.7mg/dl (N 52.6-120mg)• Serum electrophoresis- No M-band.• Cryoglobulin- Negative.• Coomb’s test Negative, dsDNA-1430 IU/ml.• ANA- Awaited.
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Case 2
? Diagnosis
Slide Seminar- I: Pathology of Glomerular Diseases - Case 2
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DISCUSSION International CME on Renal Pathology, Department of Pathology , SGPGI, Lucknow, INDIA
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Case 2
Differential diagnosis:• Lupus nephritis with massive immune complex
deposits in capillary lumina• Hep-C associated GN with cryoglobulins• Primary MPGN with cryoglobulins• Primary or secondary cryoglobulinemias• Waldenstrom’s macroglobulinemia
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Case 2Discussion• Lupus nephritis with severe lesions and without strong clinical
evidence of SLE are occasionally encountered but rare - later develop full blown clinical SLE - probably less likely
• In lupus nephritis, intraluminal “thrombi” are massive ICs and can be associated with clinical evidence of cryoglobulinemia
• EM essential for defining deposits and TRIs • Common viral infections are associated with low titer poly-specific
auto-antibodies.• High titer ANA, dsDNA, anti-cardiolipin antibodies and other
subtypes antibodies may be found.• Hep B & C, HIV and parvovirus-B19 more commonly associated
with autoantibodies than others. • Hepatitis C associated renal lesions:
• Most common renal lesion in Hep C is MPGN with or without cryoglobulins• Less common MGN, FSGS
• IF shows strong C3, others uncertain and non-conclusive
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Final Diagnosis Glomerular changes highly suggestive of MPGN
with intracapillary “thrombi”, cryoglobulins cannot be excluded.
Hep C associated GN > likely than Lupus Nephritis.
Case 2
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Follow-up• In view of strong positive anti dsDNA she was started on
steroids and pulse cyclophosphamide.• Later her HCV-RNA has become positive.• Liver function has remained normal. • Puzzled as to continue immunosuppressive therapy or to
start on interferon therapy?
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Case 2
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References:1. Hansen KE, Arnasen J, Bridges AJ. Autoantibodies and
common viral illnesses. Semin Arthritis Rheum. 1998,27(5):263-71
2. Meyers CM, Seeff LB, et at. Hepatitis C and renal disease: an update. Am J Kidney dis 2003 42(4):631-57
3. Montagna G, Piazza V, Salvadeo A. Monoclonal cryoglobulinemia in hepatitis C virus-associated, membranoproliferative glomerulonephritis. Am J kidney dis 2003Aug;42(2):430
4. Tormo A, Rivera F, Munoz C, Trigueros M. Presence of hepatitis C virus in renal tissue in membranoproliferative glomerulonephritis and cryoglobulinemia. Nefrologia. 2003;23(2):165-8. Nefrologia. 2003;23(2):165-8.
Case 2
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Case 3H/O• 41 year African American male admitted for new onset
nephrotic syndrome, and c/o pedal edema, joint pains and easy fatigability
• Past medical history of gouty arthritis and chronic renal failure.
• Meds: Lasix, Allopurinol, • Family and social history not documented. P/E• BP normal, no other findings except pedal edema and
gouty deformity of hands.
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Case 3
Lab Data: • Hb 4.0gm/dL, Hct 13%, Retic count 1.0-1.2 • Total protein 3.9 gm/dL, alb 1.8 gm/dl• BUN 49mg/dL, Creat. 3.5mg/dL Cholesterol 300mg/dL.
Electrolytes normal• U/A: Protein 3+ (7- 8 Gm/day), 3+ blood, No bacteria,
crystals or casts. • Serum - Na 149, K 5.6, Cl 120, CO2 23
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Case 3
? Diagnosis
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DISCUSSION International CME on Renal Pathology, Department of Pathology , SGPGI, Lucknow, INDIA
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Differential Diagnosis• Membranoproliferative GN (MPGN)• Membranous GN (Allopurinol-related)• Vasculitis-related lesions (Drug related)• HSP, lupus nephritis, post-infectious GN etc. • MCD and FSGS less likely.
Case 3
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Additional data• Patient was known to have sickle cell disease,
gouty arthritis and chronic renal disease.
Light microscopy• Congested, large glomeruli.• Mesangial widening with lobular accentuation
with moderate hypercellularity obliterating some capillaries, double contours
• Sickled RBCs in capillary lumina• Chronic tubulo-interstitial disease.
Case 3
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Electron microscopy• Subendothelial mesangial extension -focal and
circumferential.• Focal small subendothelial deposits• Extensive foot process effacement • Sickled RBCs in capillary lumen with Hb tactoids
with deformed shapes
Immunofluorescence microscopy • Negative for IgG, IgA, IgM, C3, C1q, fibrinogen,
albumin and kappa and lambda light chains.
Case 3
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Sickle Cell Disease• Sickle cell anemia (homozygous Hb-SS) is prevalent in AA patients.
Gene can occur rarely in Caucasians (Europe), and is found in North Africa, Middle East and India.
• Sickle cell anemia in 0.15% of AA children and Sickle cell trait in 8% in AA children.
• Disorder attributed to specific molecular lesion: substitution of valine for glutamic acid at the sixth residue of the β chain.
• Hematuria• Renal infarction and papillary necrosis, which may predispose to
UTI.• Nephrotic syndrome which may progress to renal failure• Abnormal tubular function
– Reduced concentration ability– Reduced acid and Potassium secretion– Increase uric acid and creatinine secretion– Increased phosphate reabsorption
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Final Diagnosis:• Nephrotic syndrome: FSGS, MPGN-like
changes with or without immune complexes
• Acute and chronic TID and papillary necrosis with bland scarring
• Congestion and hemorrhage
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Case 3
References1. Scheinman JI. Sickle cell disease and the kidney.
Semin Nephrol. 2003 Jan;23(1):66-76.2. Ataga KI, Orringer EP.Renal abnormalities in sickle cell
disease. Am J Hematol. 2000 Apr;63(4):205-11.3. Walker BR, Alexander F, Birdsall TR, Warren RL.
Glomerular lesions in sickle cell nephropathy.JAMA. 1971 Jan 18;215(3):437-40.
4. Pham PT, Pham PC, Wilkinson AH, Lew SQ. Renal abnormalities in sickle cell disease. Kidney Int. 2000 Jan;57(1):1-8.
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Case 4H/O• 48 year African-American female with fever, headache and
nausea
• Signs of nephrotic syndrome and HTN
• No family history of renal disease, IV drug abuser
Lab data: • U/A: proteinuria (4.5gm/d), 6-8 RBC, 5-6 WBC
• BUN 30mg/dL, Cr 3.2mg/dL, serum albumin 2.8gm/dL, glucose 108mg/dL
• Anemia (9.1gms/dL), platelets 210,000
• Serology: normal complement level, ANA neg, HIV+, Hep C virus +, Hep B virus negative, cryoglobulins negative
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Case 4
? Diagnosis
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• Pathologically, the renal lesions in HIVAN represent a constellation of glomerular and tubulo-interstitial changes, which by themselves are non-specific. They are characterized by a range of cellular and collapsing features, some leading to FSGS. They include, glomerular capillary wall wrinkling, thickening, collapse (segmental or global), variable number of large, hyperplastic visceral epithelial cells containing abundant hyaline droplets, varying degrees of segmental and global mesangial hypercellularity and dilated Bowman spaces. Sometimes the global collapsing glomerulopathy may predominate and may rapidly progress to renal failure. Striking tubular findings include tubular cell degenerative change, dilatation of the lumina reaching microcystic proportions and containing proteinaceous casts. In addition, concomitant focal to extensive, active/chronic interstitial inflammation and fibrosis is present.
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Immunofluorescence microscopy• Focal IgM, C3 staining in the mesangial and
sclerotic areas, suggesting nonspecific trapping.EM• Apart from noting tubulo-reticular inclusions
(TRIs) in the glomerular endothelial and infiltrating mononuclear cells, varied thickening of GBM and foot process effacement, no specific ultrastructural abnormalities are identified. The presence of frequent TRIs may help establish the diagnosis.
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• A spectrum of renal lesions with varied clinical presentation can occur with HIV infection. They include from tubular functional abnormalities (fluid, electrolyte and acid-base imbalance) to structural changes involving the renal parenchyma secondary to HIV associated nephropathy (HIVAN), predominantly tubulo-interstitial disease due to prerenal (ischemia, blood loss, sepsis) causes, parenchymal lesions secondary to acute tubular necrosis, interstitial nephritis, nephrotoxicity secondary to antiviral, antifungal and aminoglycoside drugs, opportunistic infections, malignancies, other forms of immune complex glomerulopathies, IgA nephropathy, amyloidosis and thrombotic microangiopathies (HUS,TTP). Other intercurrent or metabolic renal disease can also develop in these patients or may be superimposed on HIVAN.
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Case 4• Although the pathogenesis of HIVAN is not completely
elucidated, a direct cytopathic effect by the various HIV proteins on the glomerular epithelial and tubular lining cells has been considered. Indirectly, effects of viral gene products, cytokines and growth factors elaborated by monocytes and lymphocytes in response to the active infection may contribute to altered glomerular permeability and glomerular and tubular cell injury, leading to a change in the cell phenotype (dedifferentiation) and proliferation characteristics, apoptosis, thereby initiating mechanisms of glomerulosclerosis. Other factors or triggering mechanisms that may be needed to promote this process are a predisposition for glomerulosclerosis in African American individuals, viral load, host immunological factors and virus host interactions.
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• The prognosis is generally poor in patients having HIVAN with the more severe pathological lesions, in the African American population, in advanced stage of HIV infection, with nephrotic range proteinuria and higher creatinine levels at the time of initial presentation. These patients reach renal failure within months to 3 yrs. Several trials with steroids, with or without anti viral medications, ACE – inhibitors and more recently protease inhibitors have shown variable benefit in reducing proteinuria, and stabilizing the creatinine levels and thus postponing the onset of end stage renal disease.
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Case 4
DiagnosisHIV-associated nephropathy
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Case 4References1. Rao TKS, Fillipone EJ, Nicastri AD et al: Associated focal
segmental glomerulosclerosis in the acquired immunodeficiency syndrome. N Engl J Med 310:669, 1984
2. D’Agati VD, Appel GB: Renal pathology of human immunodeficiency virus infection. Semin Nephrol 18: 406, 1998
3. Madiwale C, Venkataseshan VS. Renal lesions in AIDS: a biopsy and autopsy study. Indian J Pathol Microbiol 42: 45-54, 1999.
4. Schwartz EJ, Klotman P: Pathogenesis of human immunodeficiency virus (HIV) – associated nephropathy. Semin Nephrol 18: 436, 1998
5. Ross MJ, Klotman PE. Recent progress in HIV-associated nephropathy. J Am Soc Nephrol 13: 2997-3004, 2002
6. Kimmel PL, Phillips TM, Ferrura-Centino A. HIV-associated immune-mediated renal disease. Kidney Int 44: 1327-1340, 1993
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Case 5H/O
• 55-year-old white man with a history of hypertension (on medications) and atherosclerotic heart disease for the past few years, presented with progressive edema of his legs during the last 3 months.
• No history of shortness of breath, chest pain, nausea/vomiting at this time.
• Other past medical history included gastro-esophageal reflux disease, arthroscopic knee surgery and chronic low back pain.
P/E
• Physical examination was essentially unremarkable, except for 3+ leg edema.
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Lab findings • Nephrotic range proteinuria (6.4gm/24hrs) microhematuria,
• BUN 52mg/dl,creat 2.3mg/dl
• Negative serologic tests (ANA, ANCA, HCV, HBV, RhF),
• Normal complement levels and serum protein electrophoresis.
• Renal biopsy was performed.
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Case 5
? Diagnosis
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• Based on the above clinical presentation of nephrotic range proteinuria and mild renal insufficiency in the absence of significant serologic findings and a systemic illness (infection, auto-immune, metabolic or neoplastic) in an adult points to a limited number of possibilities, such as membranous glomerulonephritis, a progressive form of focal segmental glomerulosclerosis or other forms of chronic glomerular lesions. A mild nephritic picture characterized by microhematuria, hypertension and renal insufficiency may also indicate advancing IgA nephropathy or rarely other forms of focal or early diffuse proliferative glomerulonephritis.
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Light Microscopy:• The glomeruli are variably enlarged showing diffuse, mild to
moderate mesangial expansion with mainly increase in matrix and a few cells with focal lobular accentuation and early mesangial interposition. Most of the peripheral capillary walls are thickened and PAS positive. Focal tubular atrophy is accompanied by minimal interstitial fibrosis and sparse lymphocytic infiltrate. The small arteries and arterioles exhibit moderate medial hypertrophy and intimal thickening. Special stain for amyloid (Congo red) is negative.
Immunofluorescence Microscopy:• Reveals 1+ IgG, trace-1+ IgM, 1-2+ C3 and 1+ kappa and lambda
light chains along the glomerular capillary walls and mesangial in a granular and diffuse global distribution.
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Electron Microscopy:• The glomerular capillary basement membranes show
irregular thickening and expansion of the basement membranes due to uneven dark dense deposits with fibrillar structures, occupying the lamina densa, as well as lamina rara, externa and interna. The mesangial areas are markedly expanded, with increase in cells, a modest amount of matrix and abundant deposits which have a fibrillary configuration. This fibrillar material on high magnification appears fairly long, haphazardly arranged with a solid core, occasionally having a round to stellate cross section replacing the glomerular extracellular matrix. The fibrils range in thickness from 18-22nm.
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Case 5
Diagnosis:Fibrillary Glomerulonephritis
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Differential diagnosis:• Based on preliminary examination of the renal biopsy by light
microscopy shows fairly nonspecific mesangial findings containing predominantly PAS positive matrix and some increase in cells. The lack of pale staining, homogeneous glassy material in the mesangium and glomerular capillary walls and subsequent negative staining for Congo Red excludes amyloid deposits. The inclusion of immunofluorescence data to above findings suggests immune-complex type of disease not attributable to any known etiology. However, despite a negative workup for serum and urine monoclonal proteins, a monoclonal gammopathy related glomerular disease could still be considered. Since the deposits are mainly polyclonal (IgG, kappa, lambda and C3) by IF also aids in excluding the above possibility. No significant tubulo-interstitial and vascular disease is noted. However, the application of ultrastructural examination in such a case is useful in determining the pattern, location and the nature of the deposits.
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Case 5
References:1. D'Agati V, Appel GB, Markowitz GS, Truong L, Seshan S, Kim DU,
Sacchi. Fibrillary glomerulonephritis: defining the disease spectrum. In Monoclonal Gammopathies and the Kidney, P. Aucouturier et al (eds), Kluwer Academic Publishers, Great Britain, 2003, p153-163
2. Fogo A, Qureshi N, Horn RG: Morphologic and clinical features of fibrillary glomerulonephritis versus immunotactoid glomerulopathy. Am J Kidney Dis 22:367, 1993
3. Iskandar SS, Herrera GA. Glomerulopathies with organized deposits. Sem diagn pathol. 19:116-132, 2002.
4. Schwartz MM, Korbet SM, Lewis EJ. Immunotactoid glomerulopathy. J Am Soc Nephrol 13: 1390-1397, 2002
5. Churg J, Venkataseshan VS. Fibrillary glomerulonephritis without immunoglobulin deposits in the kidney. Kidney Int 44: 837-842, 1993.
6. Bridoux F, Hugue V, Coldefy O et al. Fibrillary glomerulonephritis and immunotactoid (microtubular) glomerulopathy are associated with distinct immunologic features. Kidney Int 62: 1764-1775, 2002.
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