LC-MS/MS Solutions for Toxicology and Clinical Research
by Aymeric MorlaSenior Solutions Specialist Clinical Research, Clinical Diagnostics & Forensics South EMEA
For research use only. Not for use in diagnostic procedures.RUO-MKT11-3115-A
2 © 2015 SCIEX
Vision
SCIEX
Answers for Science. Knowledge for Life.™
MissionProvide integrated, reliable analytical tools to advance scientific understanding and safeguard health.
Placing the power of life-changing answers into the hands of those who care, everywhere.
3 © 2015 SCIEX
Pharma & Biopharma
Clinical ResearchForensics
Food & Environment
3
Life Science Research
2
The Markets We Serve
Biologics Discovery & Development Small Molecule Discovery &
Development
Proteomics Metabolomics Lipidomics Clinical Research
Contaminations & Safety Quality & Authenticity Water & Soil Testing
Toxicology Forensics Clinical Research
4
1
2
3
Services & Support for Every Customer4
1
4 © 2015 SCIEX
No. 1 LC-MS/MS Provider for Over 2 DecadesTrusted partner focused on exceeding your expectations
Continuous investment in award-winning innovations More than 20 new products since 2012 650+ issued patents or pending applications, 5+ issued patents/month 5 awards in 2014 alone
Solutions that address your entire workflow Complete portfolio of LC/MS, CE/MS solutions including methods, reagents, software and service
Partnerships to advance scientific excellence Partner with innovators and industry leaders such as; the Institute for Systems Biology, Illumina, Phenomenex, Gerstel USA and Beckman Coulter
Best-in-class global service and support More than 700 application, service and support associates deployed Worldwide Over 300 training classes available online and in person
5 © 2015 SCIEX
In the Netherlands, researchers use our CESI 8000
system for metabolomic profiling to gain insights into solving the mysteries of kidney disease.
Horse racing officials turn to the QTRAP® technologycoupled with our MicroLC to identify performance enhancing hormones.
Enabling Our Customers to Find Answers
In Singapore, researchersuse SWATH® Acquisition to identify biomarkers for inflammation of the eye.
Investigators in Japan use our TripleTOF® system to increase crop yields.
6 © 2015 SCIEX
Our History — Over 40 Years of Innovation
1973 Founded by Dr. Barry French and others at the University of Toronto
1981 Delivers the first commercial triple quadrupole MS/MS instrument
1989 Introduced API III, the first commercial dedicated LC/MS/MS system, creating the gold standard
2001 Launched API 4000 LC/MS/MS systems becomes the de facto standard for bioanalysis
2002 Released QTRAP® which combines triple quad and linear ion trap for the first time
2005 Introduced API 5000 which was the most sensitive triple quadrupole system
2008 Launched the 5500 series which was the most advanced systems of the time
2010 Became a Danaher Corporation operating company and acquired the Eksigent LC business
2012 Launched the highest number of new products in the history of the company in a single year
2013 Launched first Medical Device and IVD solution and added Beckman CE to portfolio
2014 Partnered with Illumina creating the first multi-omics cloud-computing environment
7 © 2015 SCIEXSCIEX TripleTOF® systems
SCIEX Triple Quad™ & QTRAP® systems
3500 Series 4500 Series 5500 Series & SelexION 6500 & SelexION
4600 5600+ & SelexION
Pushing the limits
MicroLC M3TM
6600 & SelexION
6500+ & SelexION+
ExionLCTM
Solutions LC-MS/MS SCIEX
X500R QTOF
8 © 2015 SCIEX
Creating Complete Solutions For Routine Analysis
Harnessing the power of Tandem MS: Best-in-class hardware + Easy to use software + Customizable applications solutions
Get customers up and running quickly
Market Specific Solution
Hardware Unique QTRAP® & QQQ Technology
Best in Class UHPLC
SoftwareCliquid® Software
ChemoView™ Software
iMethod™ Applications Specific methods for research use
MRM Catalogues and Libraries
Customer SupportInstallation and training
Phone/On-site application support
9 © 2015 SCIEX
Advantages of LC-MS/MS
• Greater selectivity and specificity
• Sensitivity
• Simplified sample preparation
• Short run times
• Multiple compound classes can be analyzed in a single run
• Inexpensive per sample costs
10 © 2015 SCIEX
• Ideal for any routine quantitative analysis
‒ Secure user-logon
‒ Simplified work-flow
‒ Preconfigured methods
‒ MRM catalogues
‒ Simplified data analysis tool
‒ Simplified reporting tool based on Microsoft word with range of pre-configured reports
‒ Available in English, Japanese and simplified Chinese by default. Additional languages will be available separately.
Cliquid® Software and iMethod™ Applications
11 © 2015 SCIEX
Cliquid® Software for Routine Analysis
For research use only. Not for use in diagnostic procedures.
12 © 2015 SCIEX
• Homocysteine in Serum
• Testosterone in Plasma
• 1,25-Dihydroxyvitamin D3 in Plasma
• Antiretrovirals in Serum
• Metanephrines in Urine and Plasma
• Amino Acids in Urine, Plasma, CSF
• Forensic Drug Screening in Blood, Plasma, Urine
• Steroids in Serum
• EthylSulfate & EthylGlucuronide in Urine
• Benzodiazepines in Urine
• Forensic Drug Quantitation in Blood, Plasma, Urine
Examples of Clinical Research and Forensic Applications
For research use only. Not for use in diagnostic procedures.
13 © 2015 SCIEX
Chromatogram of QC Control at 1.36 μg/mL
iMethod™ Applications – Homocysteine in Serum
For research use only. Not for use in diagnostic procedures.
14 © 2015 SCIEX
iMethod™ Applications – Testosterone and low levels
• iMethod™ Application for low testosterone level v1.0
‒ Testosterone method designed for samples on the 5500 systems
• iMethod™ Application for testosterone v1.0
‒ Testosterone screening method for samples using the 3200 systems
Testosterone 30 ng/mL Quality Control and D3-Testosterone (IS)3200 QTRAP® with a Shimadzu Prominence LC system in a 3 minute run
Quantifier Qualifier Int. Std.
For research use only. Not for use in diagnostic procedures.
15 © 2015 SCIEX
iMethod™ Applications – Anti-retrovirals in Serum
For research use only. Not for use in diagnostic procedures.
Overlaid MRM ion traces for the Mid QC quantifier, qualifier, and internal standard transitions performed on a 3200 QTRAP® LC-MS/MS system.
16 © 2015 SCIEX
iMethod™ Applications – Metanephrines in Urine and Plasma
For research use only. Not for use in diagnostic procedures.
Figure shows a representative chromatogram for Calibration Standard 4 (at a concentration of 100 pg/mL for each analyte) in the plasma matrix method.
17 © 2015 SCIEX
Key features:
• 45 physiological amino acids
• 18 minute run time
• Good correlation with traditional AAA techniques
• CV’s 5-10%
• For use with Plasma, Urine, CSF
iMethod™ Applications - Amino Acids Analysis
For research use only. Not for use in diagnostic procedures.
RUO-MKT-18-1999
18 © 2015 SCIEX
ILeu Leu
Ala
Beta Ala
Sar
Separation of key AA
45 Physiological AA standards at 25 pmole on column on an API 3200™ System
Example Amino Acids Chromatogram
For research use only. Not for use in diagnostic procedures.
19 © 2015 SCIEX
A
Allo Isoleucine in plasma
alloIle
Ile Leu
Nle
MSUD
Control
alloIle
Ile Leu
Nle
MSUD
ControlIle
Leu
Nle
All isomeric/isobaric AA’s resolved well
B C
D E F
B C
D E FGABA
ALA
SarALA
AibAba
Val
Nval
Ile Leu
Nle
1MHis
3 MHis
All Isomeric/Isobaric Amino Acids Resolved
For research use only. Not for use in diagnostic procedures.
20 © 2015 SCIEX
Q1
Q3/LITQ2
Q0QJet
Ion Rail of the SCIEX QTRAP® 4500 System
Key features:
• Screen for > 300 drugs
• 15 minute run time
• Confirmation by library search
• Library of over 1250 Drugs
• For blood, urine, saliva and tissue
• Via iMethod™ Applications
iMethod™ Applications - Forensic Drug Screening
For research use only. Not for use in diagnostic procedures.
21 © 2015 SCIEX
700 Forensic Drugs Screening with the 3200 QTRAP® LC/MS/MS System
Comprehensive Forensic Drugs Screening
For research use only. Not for use in diagnostic procedures.
22 © 2015 SCIEX
iMethod™ Applications – Benzodiazepines
For research use only. Not for use in diagnostic procedures.
Representative chromatogram of spiked urine at 20ng/mL performed on a 3200 QTRAP® LC-MS/MS system in a 8 minutes run.
23 © 2015 SCIEX
iMethod™ Applications – EthylSulfate & EthylGlucuronide in Urine
For research use only. Not for use in diagnostic procedures.
Sample chromatogram of MRMs of EtG and EtS detected at 100 ng/mL in a human urine sample.
24 © 2015 SCIEX
iMethod™ Applications – Forensic Drug Quantitation – Short Panel
For research use only. Not for use in diagnostic procedures.
Example chromatogram for Forensic drugs (short panel) generated on a 3200 QTRAP® system with a in a 6.6 minutes run.
25 © 2015 SCIEX
sciex.com
For more information
26 © 2015 SCIEX
CONTACT US:Aymeric Morla, email: [email protected]
AB Sciex is doing business as SCIEX.
© 2010-2015 AB Sciex.
The trademarks mentioned herein are the property of the AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license. Beckman Coulter® is being used under license.
For research use only. Not for use in diagnostic procedures.
RUO-MKT11-3115-A
