Leafy Vegetables Leafy Vegetables GGermplasm: ermplasm: SStimulating timulating UUsese

Contract number: AGRI-2006-0262Contract number: AGRI-2006-0262Agreement number: 001 AGRI GENRES Agreement number: 001 AGRI GENRES 870/2004870/2004

Partner 4 PalackyPartner 4 PalackyPalacký University in OlomoucPalacký University in OlomoucFaculty of ScienceFaculty of ScienceDepartment of BotanyDepartment of BotanyOlomouc – HoliceOlomouc – HoliceCzech RepublicCzech Republic

overview of work in the year 2007 for project meeting in Angers (France) 12-13 December 2007

Role of partner 4 Palacky in the Role of partner 4 Palacky in the project:project:

The group at Palacký University is The group at Palacký University is directly directly engaged engaged in two workpackages (WPs), in two workpackages (WPs),

WP2: WP2: Regeneration &Regeneration & characterization characterization

WP3: EvaluationWP3: Evaluation;;

Some activities are connected with tasks in Some activities are connected with tasks in WP1: WP1: DocumentationDocumentation;;

PPartner P4 develops activities leading to fulfillment artner P4 develops activities leading to fulfillment of tasks in the of tasks in the WP5: CoordinationWP5: Coordination..

Project design and deliverables:Project design and deliverables:

Partner 4 will chararecterize morphologically 30 Partner 4 will chararecterize morphologically 30 accessions of lettuce accessions of lettuce

(WP2 - to be delivered in month 45);(WP2 - to be delivered in month 45);

Partner 4 will evaluated 30 accessions of lettuce Partner 4 will evaluated 30 accessions of lettuce (identical to those used in WP2) for resistance (identical to those used in WP2) for resistance to 20 isolates of to 20 isolates of Bremia lactucaeBremia lactucae

(WP3 – to be delivered in month 45);(WP3 – to be delivered in month 45);

Obtained data will be delivered in harmonized Obtained data will be delivered in harmonized format to project database.format to project database.

WP1: DocumentationWP1: Documentation

Persons involvedPersons involved::

senior researcher: senior researcher: Aleš Lebeda Aleš Lebeda

junior researcher:junior researcher: Eva KřístkováEva Křístková

Milestone dMilestone dateate Milestone Milestone descriptiondescription

month 0month 06 6 Material selected for regeneration and Material selected for regeneration and characterization of the four crop groupscharacterization of the four crop groups

month month 12 12 Material selected for the evaluation activities of Material selected for the evaluation activities of the four crop groupsthe four crop groups

Year 1

Action:

Request for 30 accessions of old lettuce cultivars sent to curator of the Czech National Germplasm Collection – Research Institute of Crop Production (RICP) in Praha – Ruzyně www.vurv.cz;

Requested collection of accessions was provided by RICP.

WP2: WP2: Regeneration &Regeneration & characterizationcharacterization

MilestonMilestonee

ddateate

Milestone Milestone descriptiondescription Partner Partner responsible – responsible – in in format for projst format for projst reportsreports

month month 12 12 Regeneration and Regeneration and characterisation for all characterisation for all crops startedcrops started

P0, P1, P2, P3, P5, P0, P1, P2, P3, P5, P6, P7, P8, P9, P10, P6, P7, P8, P9, P10, P13P13

Year 1

MilestonMilestonee

ddateate

Milestone Milestone descriptiondescription Partner Partner responsible – responsible – harmonized by harmonized by partner 4partner 4

month month 12 12 Regeneration and Regeneration and characterisationcharacterisation for all for all crops startedcrops started

P0, P1, P2, P3, P0, P1, P2, P3, P4,P4, P5, P6, P7, P8, P9, P5, P6, P7, P8, P9, P10, P13P10, P13

Persons involvedPersons involved:: senior researcher: senior researcher: Aleš LebedaAleš Lebedajunior researchers:junior researchers: Ivana Doležalová Ivana Doležalová

Eva KřístkováEva Křístková Alžběta NovotnáAlžběta Novotná Vladimír VinterVladimír Vinter

technicians:technicians: Xenie Hanáková Xenie Hanáková Marie LechnerováMarie Lechnerová Ladislava VaverkováLadislava Vaverková Drahomíra VondrákováDrahomíra Vondráková Vladimír VšetičkaVladimír Všetička

1. Plant material (lettuce):1. Plant material (lettuce):

EVIGEZ numberEVIGEZ number Name of cultivarName of cultivar

RICP09H5700004RICP09H5700004 KamýkKamýk

RICP09H5700005RICP09H5700005 LibanLiban

RICP09H5700011RICP09H5700011 LednickýLednický

RICP09H5700014RICP09H5700014 PodřipanPodřipan

RICP09H5700019RICP09H5700019 PražanPražan

RICP09H5700021RICP09H5700021 AltenburskýAltenburský

RICP09H5700030RICP09H5700030 Pisárecký ranýPisárecký raný

RICP09H5700031RICP09H5700031 Pisárecký žlutozelenýPisárecký žlutozelený

RICP09H5700032RICP09H5700032 Hanácký letníHanácký letní

RICP09H5700034RICP09H5700034 Valašský universalValašský universal

RICP09H5700036RICP09H5700036 Malý pochoutkaMalý pochoutka

RICP09H5700040RICP09H5700040 KunovickýKunovický

RICP09H5700041RICP09H5700041 OpavskýOpavský

RICP09H5700042RICP09H5700042 Zlatý rekordZlatý rekord

RICP09H5700044RICP09H5700044 Tichotův ranýTichotův raný

15 accessions:

old, traditional Czech cultivars;

basic passport data available:

www.vurv.cz

part databases

part EVIGEZ

2. Methods (description of the work in 2. Methods (description of the work in 2007 – characterization of lettuce 2007 – characterization of lettuce germplasm accessions)germplasm accessions)

6-7 March: seeds of 15 accessions sown to Perlite;6-7 March: seeds of 15 accessions sown to Perlite; 16-17 March: seedlings transplanted to the plant beds 16-17 March: seedlings transplanted to the plant beds

with garden soil;with garden soil; 19 April: plantlets with 10-12 fully developed leaves 19 April: plantlets with 10-12 fully developed leaves

planted to the field, spaced 50 x 50 cm;planted to the field, spaced 50 x 50 cm; each accession was represented by 24 plants;each accession was represented by 24 plants; during vegetative growth of plants - morphologic during vegetative growth of plants - morphologic

characterization characterization • • for 8 descriptors according to the minimum for 8 descriptors according to the minimum descriptor list descriptor list (elaborated by the ECP/WG Leafy (elaborated by the ECP/WG Leafy Vegetables and agreeded Vegetables and agreeded for the project) for the project) and and • • for 11 additional descriptors (Křístková et al., 2007, for 11 additional descriptors (Křístková et al., 2007,

submitted);submitted); photodocumentation, sampling of achenes photodocumentation, sampling of achenes

Seed sowing to Perlit, transplanting Seed sowing to Perlit, transplanting seedlings to the blantbeds in seedlings to the blantbeds in greenhousegreenhouse

Views on Views on lettuce lettuce accessions in accessions in the field in the field in 20072007

10 August 2007

19 June 2007

Set of minimum descriptors for Set of minimum descriptors for Lactuca Lactuca sativasativa (ECP/GR, agreed by LVG in Olomouc (ECP/GR, agreed by LVG in Olomouc 2005)2005)

Descriptor nameDescriptor name Descriptor stateDescriptor state NoteNote

Seed colourSeed colour 1=white/cream, 2=yellow, 3=brown, 1=white/cream, 2=yellow, 3=brown, 4=black4=black

Flower anthocyanin Flower anthocyanin contentcontent

0=absent, 1=present0=absent, 1=present

Leaf anthocyanin contentLeaf anthocyanin content

(stand.)(stand.)0=absent, 3=weak, 5=medium, 0=absent, 3=weak, 5=medium, 7=strong7=strong

At At harvest harvest maturitymaturity

Outer leaf colourOuter leaf colour 1=yellow green, 2=green, 3=grey 1=yellow green, 2=green, 3=grey green, 4=blue green, 5=red greengreen, 4=blue green, 5=red green

At At harvest harvest maturitymaturity

Outer leaf colour Outer leaf colour intensityintensity

(stand.)(stand.)

3=light, 5=medium, 7=dark3=light, 5=medium, 7=dark At At harvest harvest maturitymaturity

Head shapeHead shape ( (in in longitudinal sectionlongitudinal section))

0=head not formed, 1=elliptic, 0=head not formed, 1=elliptic, 2=broad elliptic, 3=circular, 2=broad elliptic, 3=circular, 4=transverse elliptic4=transverse elliptic

At At harvest harvest maturitymaturity

Heart formationHeart formation

(stand.)(stand.)0=absent, 3=slight, 5=moderate, 0=absent, 3=slight, 5=moderate, 7=well developed7=well developed

At At harvest harvest maturitymaturity

HomogeneityHomogeneity

(stand.)(stand.)1=completely homogeneous, 1=completely homogeneous, 3=slightly heterogeneous, 3=slightly heterogeneous, 5=moderate heterogeneous, 7=very 5=moderate heterogeneous, 7=very heterogeneous, 9=completely heterogeneous, 9=completely heterogeneousheterogeneous

At At harvest harvest maturitymaturity

Ingeniously elaborated Czech (IECZ) Ingeniously elaborated Czech (IECZ) descriptor list for descriptor list for Lactuca sativa Lactuca sativa

germplasmgermplasm

11 additional descriptors selected from: Description of morphological characters of lettuce (Lactuca sativa L.) genetic resources E. Křístková, I. Doležalová, A. Lebeda, V. Vinter, A. Novotná (submitted to Plant Genetic Resources (CABI Publishing, UK).

The set consists of 55 descriptors and 15 of them are elucidated by figures.

Example of figure to descriptor „Leaf blade – shape of apex“:

1 truncate – 2 rounded – 3 obtuse – 4 subacute - 5mucronate

Descriptor Descriptor numbernumber

Descripton nameDescripton name Descriptor stateDescriptor state

1.3. Adult outer leaf (and leaf of non-heading types of lettuce)1.3. Adult outer leaf (and leaf of non-heading types of lettuce)

1.3.1. * 1.3.1. * Outer adult leaf - Outer adult leaf - colourcolour

1 yellow green1 yellow green

2 green2 green

3 gray green3 gray green

4 blue green4 blue green

5 red and green5 red and green

99 other 99 other

1.3.2. 1.3.2. Outer adult leaf – Outer adult leaf – intensity of colourintensity of colour

3 slight3 slight

5 moderate5 moderate

7 intense 7 intense

1.3.3.1. * 1.3.3.1. * Outer adult leaf – Outer adult leaf – anthocyanin –anthocyanin –distributiondistribution

0 absent0 absent

1 on the veins1 on the veins

2 on the blade margin2 on the blade margin

3 diffused on the entire lamina3 diffused on the entire lamina

4 in spots on the entire lamina4 in spots on the entire lamina

99 other 99 other

1.3.3.2.1.3.3.2. Outer adult leaf – Outer adult leaf – anthocyanin - anthocyanin - intensity of intensity of colorationcoloration

0 none0 none

3 slight3 slight

5 moderate 5 moderate

7 intense 7 intense

1.3.11.*1.3.11.* Outer adult leaf - Outer adult leaf - blisteringblistering

0 none0 none

3 slight3 slight

5 moderate5 moderate

7 intense 7 intense

Examples of L. sativa descriptors in IECZ:

IECZ descriptorIECZ descriptor Minimum set Minimum set ECP/GRECP/GR

DescriptoDescriptor numberr number

Descriptor Descriptor namename

Descriptor Descriptor statestate

Descriptor Descriptor statestate

Descriptor Descriptor namename

1.6.2.1. 1.6.2.1. Flower – Flower – anthocyanin – anthocyanin – distribution distribution pattern on pattern on lower part of lower part of ligules ligules

0 absent0 absent

1 in spots1 in spots

2 on margin2 on margin

3 diffused on 3 diffused on surfacesurface

99 other 99 other

0 absent0 absent

1 present1 present

Flower Flower anthocyanin anthocyanin contentcontent

Descriptor for „ Flower, resp. Descriptor for „ Flower, resp. Inflorescence“ Inflorescence“

Example of harmonization of obtained data

4. Results4. ResultsExamples of evaluated Examples of evaluated characteristicscharacteristics

cultivar Liban cultivar Kamýk

1. Uniformity in basic characteristics assessed

2. Heterogeneity in head formation within 2. Heterogeneity in head formation within cultivar Pražancultivar Pražan

3. Symptoms of Edge burn on cultivar 3. Symptoms of Edge burn on cultivar PražanPražan

4. Necrosis of flower buds - cultivar Zlatý 4. Necrosis of flower buds - cultivar Zlatý rekordrekord

symptoms expressed only on several plants

5. Heterogeneity in developmental stages 5. Heterogeneity in developmental stages

within accessionswithin accessions

Kunovický

50:50

Opavský

1 plant

6. Differences in developmental stages among accessions

WPWP33: : EvaluationEvaluation

MilestonMilestonee

ddateate

Milestone Milestone descriptiondescription Status Partner Status Partner PalackyPalacky

month month 1818 Evaluations startedEvaluations started Started in month 6Started in month 6

Year 1

Persons involvedPersons involved:: senior researcher: senior researcher: Aleš LebedaAleš Lebedajunior researcher:junior researcher: Irena Petrželová Irena Petrželová

Action:Action:• totally 30 isolates of totally 30 isolates of Bremia lactucaeBremia lactucae controlled in controlled in

pathogenicity tests in growth chamber on a basic set of pathogenicity tests in growth chamber on a basic set of differential lettuce genotypes differential lettuce genotypes

• 20 isolates pre-selected for evaluation in year 2 (2008)20 isolates pre-selected for evaluation in year 2 (2008)

WP5. WP5. Co-ordinationCo-ordination and and disseminationdissemination

Persons involvedPersons involved:: senior researcher: senior researcher: Aleš Lebeda Aleš Lebeda junior researcher:junior researcher: Eva KřístkováEva Křístková

Action:Action: participation at meetings, elaboration of projects participation at meetings, elaboration of projects

reportsreports, financial agenda, financial agenda Article on LeafyVeg Project was translated into Czech Article on LeafyVeg Project was translated into Czech

and sent and sent to to „Žurnál“ - „Žurnál“ - official official journal ofjournal of Palacký Palacký University in OlomoucUniversity in Olomouc and provided to expert group and provided to expert group „MedVěd“ which is responsible for communication „MedVěd“ which is responsible for communication with media and spreading information on University with media and spreading information on University research activities to publicresearch activities to public

Other issues:Other issues:

E. Křístková, I. Doležalová, A. Lebeda, V. Vinter, A. Novotná: Description of morphological characters of lettuce (Lactuca sativa L.) genetic resources (submitted in 2007 to Plant Genetic Resources (CABI Publishing, UK)).

CommentsComments

no problems with the study of lettuceno problems with the study of lettuce no bottlenecksno bottlenecks difficulties with co-financingdifficulties with co-financing external audit – much money to be external audit – much money to be

payied for this action not closely payied for this action not closely connected to the project itself connected to the project itself

Euro/CZK – not possible to keep exactly Euro/CZK – not possible to keep exactly what was planned in each categorywhat was planned in each category

We thank you for your kind attention. We thank you for your kind attention.