7/23/2019 Lecture #5. Maintenance of QC Organisms_Indonesia 2015

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Maintenance of Quality ControlStrains

7/23/2019 Lecture #5. Maintenance of QC Organisms_Indonesia 2015

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OUTLINE

• Selection of Strains for QC

•  Testing

•

Storage• Maintenance

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Selection of Strains for

QC•

Reference stock cultures should beobtained from a recognized source (e.g.ATCC) a!ailable in l"o#hilized state

• Recommended QC strains and e$#ected

QC ranges (C%S& M')Routine QC strains* and su##lemental QCstrains*

• +sers of commercial s"stems should follo,the QC recommendations in that s"stem-sinstructions for use

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/

 

Commercial l"o#hilizedstrains are stored at 201oC intheir original #ackage untile$#ir" date

Selection of Strains for

QC (2)

7/23/2019 Lecture #5. Maintenance of QC Organisms_Indonesia 2015

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C%S& M'0S2 3A45 //

Selection of Strains for

QC (3)

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Testing of QC Strains

• QC strains are tested regularl" dail"

 ,eekl"

 ne, batch6lot or shi#ment

• Al,a"s use fresh organisms for QCtesting

• Select isolated colonies for the test

• 7o not test colonies from acontaminated #late

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Testing of QC Strains (2)

• A frozen or l"o#hilized QC organismshould be started on a non0selecti!eagar (TSA9 :A3)

• 7o not test the original #ellet #late.

 This is the #late on ,hich thefrozen6l"o#hilized #ellet ,asstarted

 The organisms gro,ing on this#late are not full" resuscitated.

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Storage of QC Strains

3ro#er organism storage andmaintenance is re<uired to ensureacce#table #erformance

• %"o#hilized (commercial)9 store at 201oC

• =or long0term storage9 store at 08oC

Medium> '0'? gl"cerol in tr"#tic so"broth9 skim milk9 de@brinated shee# blood

+se cr"ogenic !ials (cr"o!ials)

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Storage of QC Strains (2)

At minus 8oC9microorganisms incr"o#reser!ati!e medium canbe stored for se!eral "ears

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=or short0term storage>• Store aerobic bacteria at 201oC

• Store fastidious organisms at room

tem#erature or at oC in CB2

• Store Cam#"lobacter at oC (/2oC)in microaero#hilic conditions

•  Store anaerobes in anaerobicconditions at 201oC

'

Storage of QC Strains (3)

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''

Microorganism Maintenance 3lan A

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'2

Microorganism Maintenance 3lan :

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  Thank you

'