Date post: | 19-Jan-2016 |
Category: |
Documents |
Upload: | gregory-jones |
View: | 219 times |
Download: | 0 times |
Lecture 9 Designed self-assembly
with Biomolecules
DNA
Parameters of A-, B-, and Z-DNA Double Helices (after Dickerson, et al., 1982)
Property A-Form B-Form Z-FormHelical sense Right-handed Right-handed Left-handedRepeating helix unit 1 base pair 1 base pair 2 base pairsRotation/base pair (degrees) 33.6 35.9 +/- 4.2 -60/2Mean base pairs/turn 10.7 10.0 +/- 1.2 12Inclination of base normals to helix axis (degrees) +19 -1.2 +/- 4.1 -9Rise/base pair along helix axis (Å) 2.3 3.32 +/- 0.19 3.8Pitch/turn of helix (Å) 24.6 33.2 45.6Mean propeller twist (degrees) +18 +16 +/- 7 ~0
Occurrence Dehydrated Most common Rare - certainin solution sequences
http://www.clunet.edu/BioDev/omm/dna.htm
(Needs chime plugin)
Different DNA structures
Copying DNA - the polymerase chain reaction
Copying DNA - the polymerase chain reaction
Copying DNA - the polymerase chain reaction
Attaching things to DNA
1. Biotin Streptavidin interaction - generic molecular adapters2. Thiols - Nanoparticles3. Fluorohores - for sensitive detection4. Proteins - protein/DNA recognition5. Proteins - semi-synthetic conjugation6. Metal - metallisation for conductors
DNA detection using nanoparticle assembly
Chad Mirkin Thiol terminated ssDNA
Sensitivity - femtomol(ar)Selectivity - 100,000 : 1 for point mutations (single base pair changes)
Chad Mirkin
DNA detection using nanoparticle assembly
Chad Mirkin
Using DNA bar codes to detect proteins
Science 2003, 301, 1884-1886.
Chad Mirkin
Using DNA bar codes to detect proteins
Science 2003, 301, 1884-1886.
3 aM
30 aM
Sensitivity
aM = attomolar = 10-18M
Niemeyer DNA protein conjugates - ImmunoPCR
Protein diagnostics using DNA
DNA as a scaffold for something else
Biotin Streptavidin interaction - generic molecular adapters
Niemeyer DNA directed immobilisation (DDI)
DNA as a scaffold for something else
Niemeyer
Protein directed DNA organisation
QuickTime™ and aGIF decompressor
are needed to see this picture.
Chains Rings Networks
Ionic strength dependent supercoiling
Chembiochem. (2001) 2, p260-4
DNA directed Protein organisation
Niemeyer Enzyme localisationChemBioChem (2003) 2, p242-245
DNA metallisation
Braun, and others Nature (1998) 391, p775-8
DNA metallisation - use of protein masks
Braun, and others Science, (2003) 302, p1380-1382
DNA superstructure metallisation
Finkelstein and others
Yan et al Science (2003) 301 p1882
Ribbons
Finkelstein and others
Yan et al Science (2003) 301 p1882
DNA superstructure metallisation
Grids
DNA superstructures for positioning proteins.
Finkelstein and others
Yan et al Science (2003) 301 p1882
Streptavidin locations determined by Biotin labelled DNA grids
Peptide nanotubes - a silver cloud with a peptide lining
Reches and Gazit Science (2003) 300, p625