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1 L’Efficacia delle Soluzioni saline elettrolizzate in Agricoltura AIDB 2015, October 2 nd – Politecnico Milano Bicocca Mariachiara Benedetto
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L’Efficacia delle Soluzioni saline elettrolizzate in AgricolturaAIDB2015, October 2nd – Politecnico Milano Bicocca
Mariachiara Benedetto
De Nora profile – 2272 Patents
Founded in Italy in 1923 by Oronzio De Nora, a genial chemical engineer, a successful inventor and entrepreneur, strategically expanded worldwide in all continents with direct establishment, joint ventures and acquisitions
Industrie De Nora S.p.A., the holding of De Nora group, is a private company fully controlled by the De Nora family
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De Nora Next:
a business unit looking at the future
Mission
• contribute to Well Being improving quality oflife for: Individuals, Families, People and thePlanet
• implement innovative, sustainable, on demandelectrochemical product and systems
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• be one of the future growth factor for De Nora
Objectives
Bring our electrochemical technological excellenceacross different markets and geographies withsolid, strong business partnerships andacquisitions
Project history2006 First approach and communication from Prof. Achille De Battisti –
University of Ferrara• The Idea was to apply Free Available Chlorine for Trees treatment• A dedicated patent was deposited and maintained along these years
2007 Four times the Project was presented to the De Nora Board ofDirectors receiving green light to proceed
• Many times De Nora met University Professor, Researchers andPeople involved in Agriculture
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People involved in Agriculture• A University Spin-off was decided as a New-Company were develop
the application of the Elecytrolyzed Water
2008 Verdenora University of Ferrara Spin-off company started
• KCl electrolysis process was designed
• Prototypes for Testing were prepared and distributed
• Existing EVA System was designed based on Data from the Field andproduced in 2009
Related PatentsElectrochemical device for biocide treatment in agricultural applicationsApplication number: 20100183745-
The invention relates to an electrochemical device which simultaneously carries out the
production of an oxidising solution at controlled composition containing hypochlorous acid
and the sprinkling thereof in a continuous process, useful for biocide treatments in
agricultural fields. The device can be installed on motor vehicles or mobile means in...
Inventors: Paolo Rossi, Mariachiara Benedetto, Luca Buonerba, Achille De Battisti, Sergio
Ferro, Fabio Galli (Industrie De Nora S.p.a.)
SYSTEM FOR ELECTROCHEMICAL GENERATION OF HYPOCHLORITEApplication number: 20130087449
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ELECTRODE FOR ELECTROCHLORINATIONApplication number: 20130087450
Abstract: The invention relates to an electrode for electrochemical generation of hypochlorite.
Assignee: Industrie De Nora S.p.A.
Inventors: Antonio Lorenzo Antozzi, Mariachiara Benedetto, Alice Calderara, Chiara Pezzoni,
Christian Urgeghe
Application number: 20130087449
Abstract: The invention relates to a system for a point-of-use electrochemical generation of
hypochlorite on demand in a wide range of volumes and concentration.
Assignee: Industrie De Nora S.p.A.
Inventor: Mariachiara Benedetto
Why Electrolyzed Water?
Electrolyzed water produced on site from natural raw materials, safeguard the
environment before and after its use.
Electrolyzed water is produced by the electrolysis of ordinary tap watercontaining the salt Potassium chloride
back to salt and water
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• No persistence in the environment(Environmentally friendly )
• No surplus production
• Effective against fungi and bacteria
• Less resources exploitation
• Less fuel consumption and transport costs
• Less plastic and chemicals to be disposed
SALT WATER
ELECTRICITY ON SITE PRODUCTION AT FARM
ELECTROLYZED WATER FOR FARMER USE
Agriculture
• Our technology is based on patented electrochemical system producing the Potassium Hypochlorite solution on site and on demand, aimed at preventing and treating certain fruit tree diseases.
• The effectiveness of Hypochlorite ion is well known.
• To promote a new type of crop.
• To sanitize fruits and vegetables for the customer without pathogens
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• To sanitize fruits and vegetables for the customer without pathogensinside and without pesticide residuals.
• Potassium is one of the major nutrients for plants.
• Potassium hypochlorite is not phytotoxic on fruit trees and on vegetables
• Has been demonstrated that Electrolyzed Water is successful in croptreatment.
Project Partners
� University of Ferrara� University of Bologna� University of Tuscia� Ospedale Sacco - Milan
OTHER CONSULTANTS: � RTC- Research Toxicology Center� Agrofarma (National Pesticides Company Association of Italy).
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� Chemservice� Centri di Saggio (official trials testers): Italy (BETA, AGREA, SPF) and Germany
(DLR e Koga).� Other testers involved in the project:
� Italians testers� The Netherlands testers� Spain testers� France testers
De Nora Next project recognized as one of the 10
best sustainable practices by EXPO MILANO 2015.
On July 8th De Nora NEXT will present its project “Ozone
and electrolyzed water: green innovation for a paradigm
shift in sustainability, quality and safety of mediterranean
agri-food products “ at the Conference Center of EXPO for
the Best Sustainable Development Practices Week.
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VI WORLD CONGRESS OF AGRONOMISTS
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CENTRE INTERNATIONAL DE HAUTES ETUDES AGRONOMIQUES MÉDITERRANÉENNES
1962 - General Secretariat, ParisMAI-Montpellier, MAI-Bari, MAI-Zaragoza, MAI-Chania
Dr. Thaer YASEEN
Thaer YASEEN
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Thaer YASEEN
• Citrus• Cherry
Cherry Citrus
15 September, 2015 – EXPO Milan, Italy12
• Grapevine•• Apple•• Pear
GrapevineApple
Treatments:Winter time (opening and closing season) and Spring time
CANKER due to Nectria Galligena
Block of trunk canker and
formation of a scar
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©Copyright 2013 Industrie De Nora S.p.A.Dati sperimentali preliminari - Il documento è da intendersi incompleto senza i commenti specifici della presentazione
Agust 2008 September 2008 May 2009 September 2009
April 2010 March 2011 November 2011 April 2012
Article: VALUTAZIONE DELL’EFFICACIA DELL’ACQUA ELET TROLIZZATA VERDEVIVA NEI CONFRONTI DI NEONECTRIA GALLIGENA SU MELO E DI ERWINIA AMYLOVORA SU PERO. V. FERRI1, M.COLLINA2, R. EPIFANI2, A. BRUNELLI2
Formation of new wood and
closing of canker wounds
APPLE & PEAR
EUROPEAN CANKER Neonectria Galligena
APPLE SCAB Venturia
inaequalis
GLOESPORIUM ROT Gloesporium spp.
BACTERIAL FIRE BLIGHT Erwinia amylovora
15 September, 2015 – EXPO Milan, Italy14
CHERRY
Monilinia spp. Botrytis cinerea
15 September, 2015 – EXPO Milan, Italy15
Monilinia spp. Botrytis cinerea
PRE HARVEST TREATMENT BY E.W. DEMONSTRATED REDUCTION OF FUNGI
15 September, 2015 – EXPO Milan, Italy16
CITRUS
Post harvest applicationPenicillium spp.
Field application
15 September, 2015 – EXPO Milan, Italy17
application
POST HARVEST WASHING BY E.W. DEMONSTRATED REDUCTION IN PATHOLOGY INCIDENCE AND GOOD CITRUS QUALITY
Step 3Step 2Step 1
15 September, 2015 – EXPO Milan, Italy18
Wounding the fruits
Penicillium ItalicumInoculation
Washing the fruits Conservation at 23° Cfor 7 days
�Electolyzed water at
different concentrations
�Washing time: up to 5 min
�Replicate box: 2-4
REDUCTION IN PATHOLOGY INCIDENCE …
5 min EW High concentration
5 min EW Medium concentration
15 September, 2015 – EXPO Milan, Italy19
5 min control
5 min Low concentration
Low concentration Medium concentration High concentration Control
a a aa a a
bc
b
cbc
b
c
0
5
10
15
20
25
30
Fu
ng
i CF
U/g
of f
ruit E.W.
Control
….REDUCTION OF FUNGI AND GOOD CITRUS QUALITY IN HARDNESS AND SUGAR CONTENT
15 September, 2015 – EXPO Milan, Italy20
0 day 10 days 20 days
Time
0
Fu
ng
i CF
U/g
of f
ruit
bab
a
bab
a
cd
cdd
cd
cdd
0 day 10 day s 20 day s
Time
8,89,0
9,29,49,69,8
10,010,2
10,410,6
10,811,0
Su
ge
r co
nte
nt (
bri
x)
A . E. Contr ollo
b
cd
a
b
cd
a
cd
de
e
cd
de
e
0 day 10 day s 20 day s
Time
1,4
1,6
1,8
2,0
2,2
2,4
2,6
2,8
Har
dnes
s (N
)
E.W. Control
PRE HARVEST APPLICATION OF E.W. ON APPLE CV PINK RESULTED IN A 10 % TO 20% INCREASE OF WHOLESOME PRODUCT AFTER 4 MONTHS OF REFRIGERATION.
• E.W. applied from end June every 7-10days up to end of October
• STD pesticides applied as usually by farmer from end of June to October
• Harvest: 30th October
• Preservation: controlled atmosphere and dynamic atmosphere refrigeration
+12%
Healthyapples
98%87%
Apples affected By Gloesporium Rot
15 September, 2015 – EXPO Milan, Italy21
and dynamic atmosphere refrigeration
• Sampling: 16th March
E.W. CONVENTIONAL
E.W CONVENTIONAL
2011 • APPLE - PINK LADY
Thesis - Pesticide usually employed by
farmer up to half-July, then treatment
ONLY with E.W. up to harvesting
survey: multi-residual on 20 fruits
2011 • PEAR - ABATE FETEL
Thesis - Pesticide usually employed by
farmer + E. W from springsurvey: multi-residual on 20 fruits
TREATMENT WITH ELECTROLYZED WATER ALLOW SREDUCTION OF PESTICIDES RESIDUALS, WHILE FRUITS ARE IN HEALTHY CONDITIONS
STANDARD PESTICIDE
E.W.
RESIDUALS 4 1
STANDARD PESTICIDE
E.W.
RESIDUALS 5 2
ActivePrinciple
Quantitymg/kg
Quantitymg/kg
15 September, 2015 – EXPO Milan, Italy22
Active PrincipleQuantitymg/kg
Quantitymg/kg
Dithianon 0.199 0.151Boscalid 0.073Pyraclostrobin 0.008Captan 0.108
STANDARDPESTICIDE
E.W.
RESIDUALS 3 2
2014 • GRAPES
Principle mg/kg mg/kg
Iprodione 0.319 0.645
Dithiocarbamate 1.14 0.223
Tetraconazole 0.005
Captan 0.095
Kresoxim methyl 0.006
8,00
Nu
mb
er
infe
cte
d s
ho
ots
/plo
t
Field Test on pear cv Abate Fétel - Reggio Emilia (Italy) 2010
E.W UNTREATED
AFTER TREATMENT WITH E. W. THERE WASN’T EVIDENCEOF FIRE BLIGHT INFECTION (ERWINIA AMYLOVORA) IN PEAR ORCHARD (ABATE FÉTEL)
15 September, 2015 – EXPO Milan, Italy23
No Infection
appearance
0,00
1,00
2,00
3,00
4,00
5,00
6,00
7,00
8,00
Untreated E.W. 150 ppm E.W. 300 ppm Copper
oxychloride
(Cupravit blu
WG)
Acilbenzolar-s-
methyl
(Bion)
Nu
mb
er
infe
cte
d s
ho
ots
/plo
t
21/5 6/8
E.W. Low
conc.
E.W. Very Low
conc.
GRAPEVINE
Downy mildewPlasmopara viticola
Gray mold Botrytis cinerea
15 September, 2015 – EXPO Milan, Italy24
Botrytis cinerea
A- Control B- Conventional C- E.W.
TREATMENT WITH ELECTROLYZED WATER ALLOW THE REDUCTION OF FUNGI ON VINE LEAFS, DOWNEY MILDEW ON BERRIES MAINTAINING QUALITY OF GRAPEVINE
5
3
4
5
6 5,45,1
4,8
3,9
4,74,74,6
5,9
CF
U/g
Le
af
(lo
g)
60
80
10098,9%
%
15 September, 2015 – EXPO Milan, Italy25
Aida Raio - CNR – Istituto per la protezione delle piante- Firenze
0
1
2
3
YEASTFUNGIBACTERIA
E.W+ CONVENTIONALCONVENTIONALCONTROL
CF
U/g
Le
af
(lo
g)
0
20
40
E.W+
CONVENTIONAL
2%
CONVENTIONAL
7%
CONTROL
%
TESTS DEMONSTRATED ELECTROLYZED WATER ISN’T TOXIC FOR HONEYBEEN AND IT HASN’T REPELLENT EFFECT
Apis mellifera L.
10 Apis mellifera
(5 replicate)
Difference in mortality not significative No mortality
100 93,33%
Oral Toxicity
80
90 84%
Toxicity from contact
15 September, 2015 – EXPO Milan, Italy26
0
20
40
60
80
E.W.
10,42%
CONTROL
0%
REFERENCE
(Dimethoate)
Mo
rta
lity
%
0
10
20
30
40
50
60
70
80
E.W.
0%
CONTROL
0%
REFERENCE
(Dimethoate)
Mo
rta
lity
%
Electrolyzed Water for FarmingGROWING
NURSERY
ROOMS
REPRODUCTION
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FOOD
PIPELINE
Weaning
Sanitization Test in Nursery room
STANDARD practice for Nursery rooms sanitization is to wash and rinse all in one step;
foaming surfactant “Schiuma C66”. High pressure water rinse – Operation time 2.5 – 3 h
Rinsing with Soleva
500 ppm - 6 min
Sample 0
Rinsing with water
Sample 0( Before treatment)
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Sample 0 ( Before treatment)
Sanitization (day afeter):
Soleva @2000 ppm
Sample 1(After sanitization)
Sanitization (day after):
Professional 5/1
Sample 1(After sanitization)
Sanitization Test
2000
2500
3000
UFC
/cm
qTotal Bacteria Charge (TBC)
Standard Product Soleva
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0
500
1000
1500
1 2 3 4 5 6 7 8 9 10
CBT /UFC prima sanificazione 1620,6 1725,4 1375 2536 2050 203 90,8 26,2 365,4 61,2
CBT/UFC dopo sanificazione 2 3,6 2 11,6 2,4 0,6 1,8 2,6 1,8 5,8
UFC
/cm
q
