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Lentikats Lentikats B B iotechnology iotechnology in in O O rganic rganic A A cid cid P P roduction roduction
Lentikats Lentikats BBiotechnology iotechnology in in OOrganic rganic AAcid cid PProductionroduction
Content
• What is the Lentikats biocatalyst?• How is the Lentikats biocatalyst produced?• Benefits of the Lentikats biotechnology • Lentikats biocatalyst and its usage in organic acid pr
oduction• Partners• Guarantees• Contacts
What is the Lentikats Biocatalyst?Unique shape of the matrix and original immobilization procedure enables to encapsulate free enzymes or microorganisms (bacteria, yeasts, fungi) inside a solid porous carrier from polyvinylalkohol (PVA). The advantages of PVA cells are:
• Excellent physical and mechanical characteristics that result in a long-term mechanical stability
• Hardly biodegradable• No toxic effects
Lentikats biocatalysts enable easy separation of enzymes or microorganisms from the bulk media and therefore can be used repeatedly.
Biotechnologies are changingthe world…
How does the Lentikats Biocatalyst work?
A maximum surface in combination with the minimum volume of Lentikats biocatalyst results in a substantial reduction of diffusion limitations associated with the transfer of substrate into the matrix and the product out of the matrix.
Why polyvinylalcohol?
• It is an inexpensive material
• Encapsulation takes place under optimal, cell-sparing conditions
• It allows a production of hydrogel with excellent mechanical properties
• It does not have any side effects on fermentation
• It offers high capacity for the cell loading
• The process is stable and easy to control
• It is a non-toxic material
Biotechnologies are changingthe world…
How is the Lentikats Biocatalyst produced?
What can be immobilized?
• Free or immobilized enzymes
The size of Lentikats biocatalyst pores varies depending on the size and diameter of the biocatalyst due to:
• type of PVA additives (size of macromolecules)• concentration of additives• biomass concentration
• Free cells
• Dead cells• Living non-growing cells• Living growing cells
Laboratory Units for the Encapsulation
• Lab scale units with a low production capacity of Lentikats biocatalysts (for research purposes) are also available
Lentikats Biocatalyst – production line
What are the benefits of LB?
Biotechnologiesthat are environmentally friendly …
…and also…
• Lower biochemical reaction time
• In comparison with conventional technologies much higher yield (i.e. 5 – 15% higher)
• Reuse of the biocatalyst (sieve separation)
• Reduction of the risk of contamination during fermentation processes
• High stability of production processes
Lentikats Organic Acid Production Solutions
Lentikats biotechnology is used in the following processes:
• lactic acid production from glucose, sucrose and lactose (immobilized bacteria Bacillus coagulans)
• gluconic acid production from sucrose and glucose(immobilized filamentous fungi Aspergillus niger)
• itaconic acid production from sucrose and glucose(immobilized filamentous fungi Aspergillus terreus).
Lentikats BiotechnologyLENTIKATS BIOTECHNOLOGY FOR LACTIC ACID PRODUCTION
Producer: bacteria Bacillus coagulans.
Substrates: glucose, sucrose, lactose.
Production: • batch, fed batch and continuous lactic fermentation is stable for all C-substrates.
• saccharose fermentation solutions with 60 g/l of saccharose concentrate (The total fermentation process was decresed from 48 hours in „the first conversion“ to 12 hours in „the tenth conversion“ with a lactic acid yield of 91 to 93 %.)
• glucose fermentation solution with 60 g/l glucose concentrate (After 14 hours fermentation broths consist of 55.2 g /l of lactic acid and 1.2 g /l of glucose in the tenth conversion.)
• fermentation productivity is increased from 13.5 to 17.0 (g LA.dm-3.h-1).
Lentikats BiotechnologyLENTIKATS BIOTECHNOLOGY FOR ORGANIC ACIDS PRODUCTION
Producer: 1) filamentous fungi Aspergillus terreus for itaconic acid production2) filamentous fungi Aspergillus niger for gluconic acid production
Substrates: sucrose, glucose
Production: • extensive production of itaconic acid is not stable yet
• first results indicate that if the initial concentration of sucrose is 50 g/l then the concentration of itaconic acid is 18.5 g/l in the batch mode process
What does Lentikats Biocatalyst bring?
High specific activity
Fermentation processes regulation
Operational costs decrease
Optically clear L-lactic acid (99%) manufacturing
High yield of lactic acid (about 20 %)
RobustnessBiomass regeneration costs decrease
Delivering on Lentikats Biotechnology Key Benefits
High Productivity
Short fermentation time (10 hours)
High yields
About 91-93 %
Reusable
Lentikats Biocatalysts High concentration of
Lentikats Biocatalysts in fermentors
Low risk of contaminatio
n
Increased operational costs
Decreased sales revenue
Decreased fermentation
volumes
Notable cost reduction
Significant improvement of
operating economic results
Our partners in the field of industrial applications
geniaLab®, BioTechnologie-Produkte und Dienstleistungen GmbhBüchnerstraße 7, 38118 Braunschweig, Germany
www.genialab.com
Lentikats Biotechnology – guarantee of your investments and the investment
return• You can test our biocatalyst easily by yourself with your substrate
in your own plant or plant of your choice to see the results.
• We offer you our assistance and full support.
• We offer a long-term cooperation to the engineering companies specialising in the design of distilleries and are interested in the implementation of technologies requiring minimal investment at high profitability.
Biotechnologies are changing the world…
Lentikats Biotechnology – Investment and Investment
Return GuaranteesWe guarantee for the following:
1. delivery of production facilities within 24 months2. delivery of output parameters3. operational stability of Lentikats biocatalyst and their
activity and productivity4. purchase price stability within 36 months.
Our partners in the field of research and development
Institute of Microbiology, Academy of Science of the Czech Republic,Vídeňská 1083, 142 20 Praha 4, Krč
www.biomed.cas.cz
STU Bratislava, Faculty of chemical and food technology, Institute of biotechnology and food technology, Radlinského 9, 81237 Bratislava, SK
www.stuba.sk
ICT Prague, Department of Fermentation Chemistry and bioengineering, Technická 5, 166 28 Praha 6
www.vscht.cz
University of Alberta, Edmonton Alberta, T6G 2J9 Canada
www.ualberta.ca
Lentikats Biotechnology Applications
Lentikats Biocatalysts and applied Lentikats Biotechnology also delivers additional value and benefits in the following sectors:
• waste and industrial water treatment, • distillery industry – bioethanol production,• pharmaceutics – production of robust biocatalysts,• food industry – whey processing, production of special
sugars carbohydrates and syrups
Biotechnologies are changing the world…
ContactsCompany Headquarters:
LentiKat‘s a.s.Office Building ARGOEvropska 846/176a160 00 Prague 6Czech Republic
Tel.: + 420 255 710 680Fax: + 420 255 710 699E-mail: [email protected]
Production plant:
LentiKat‘s a.s.Pod Vinicí 83471 27 Stráž pod RalskemCzech Republic
Tel.: + 420 487 851 393Fax: + 420 487 851 399E-mail: [email protected]
Laboratory:
INBIT LentiKat‘s a.s.Kamenice 34625 00 BrnoCzech Republic
Tel.: + 420 511 205 288Tel.: + 420 511 205 287
