Date post: | 17-Jul-2015 |
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Facilitating tissue collection for translational research
Jane Rogan
Business Manager
MCRC Biobanking: The Basic Concept
Make quality tissue collection and retrieval easy for the researcher
• Regulatory Framework
• Sample Collection Logistics
• Sample Quality
• Informatics
• Sample Access
Collaborating Trusts
MCRC Biobank
Pennine Acute
Hospitals Trust
The Christie NHS
Foundation Trust
South Manchester
NHS Foundation
Trust
Salford Royal NHS Foundation
Trust
HPB Breast
Colorectal Skin Urology Gynaecology Haematological Malignancies
Lung Breast Head & Neck Colorectal
Breast Upper GI Urology Neurology
Building a successful Biobank
3%
15%
1%
7%
11%
11%
1%
18%
33%
Collections By Disease Type - Solid Tumours
Brain Breast Sarcoma Skin Colorectal
Upper GI Gynae Urology Lung
Donations from over
6000 patients
47%
11%
10%
32%
Collections By Disease Type - Blood Disorders
AML B-ALL MDS Other
Donations from 400 patients
Biobank Project Applications
1
13
5
4
5
2
11
6
3
1
4
8
23
Gynae
Breast
Colorectal
Upper GI
GU
Lymphoma
Haematology
Lung
Head and neck
Neuro
Melanoma
Mixed Sample Types
QC/Method Development
0 5 10 15 20 25
Total Applications: 86 Total Approved: 75
Average Approval Time: 30 working days (median)
The “Six-Pack”
1. Fresh Frozen Diseased Tissue
2. Fresh Frozen Non-involved Tissue
3. FFPE Disease Tissue
4. FFPE Non-involved Tissue
5. Pre-operative Blood sample
6. Pre-operative Urine sample
Melanoma Tissue Collection Pathway
Collect Fresh From Theatres
Fresh Cut-Up in Pathology Diagnostic Sample Research Sample
Mouse model of tumour
Frozen Tissue for Nucleic Acid Extraction
Fixed Tissue for Staining
Evolving the Collections
Requirement Considerations Prospective Fresh Tissue • Availability of tissue
• Staff available for sample delivery
• Media for keeping samples viable
Use for Animal Models • Specific consent for animal models
• Size of tumour tissue
Alternative Sample Types (fluids, plucked hair)
• Routes / logistics of sample collection
Serial Blood Collection inc CTCs • Biobank Technician presence in clinic
Past samples (e.g. pre-treatment/primary tumours)
• Specific consent
• Requesting blocks from local hospitals
Sampling tumour in different areas • Further input from pathology
Clinical Trial Sample Collection • Space for sample storage
• Different protocols for each trial
Biobank Projects by Type
17
39
10
8
Fresh Tissue
Banked Samples
Archive FFPE/TMA
Prospective Protocol
Total Approved Projects By Type
0
2
4
6
8
10
12
14
16
Fresh Tissue
Banked Samples
Archive FFPE/TMA
Prospective Protocol
Active Projects By Type (Total 33*)
Tissue Microarrays
• Current disease areas include prostate, bladder, lung • Link to clinical databases • Recently obtained Section 251 Support which allows the Biobank
to link archival samples to clinical data • Support through Surgical Oncology Research Theme – Data
Manager employed
External Projects utilising Biobank Resource
• Stratified Medicine Programme – Phase 2 – Lung focus
– Biopsy samples from late stage patients
• 100k Genome Project – Lung, Colorectal, Prostate
• TracerX – Facilitating sample collection
• MesoBank – Mesothelioma
Research Biopsies
• Working with clinical teams to introduce sample collection as a research only procedure
• How tumours change over time
• Comparing primary and metastatic tumours in patients
• Research into why patients are relapsing on targeted treatments
• Pilot to being in Malignant Melanoma - 2014
Key Success Drivers
• Established network model with existing infrastructure and pathways for sample collection
• Excellent clinical engagement across all cancer disease types
• Robust governance framework for tissue and data
• Proven track record of providing quality samples
• Research needs drive the sample collection, not the other way round