+ All Categories
Home > Documents > LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S....

LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S....

Date post: 22-Mar-2020
Category:
Upload: others
View: 13 times
Download: 0 times
Share this document with a friend
35
LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. Amino acid mix (20 AA) "Cell free" Isotopic amino acids,chemical purity (96-98%) (Cambridge Isotope Laboratories, Inc, cat # NLM-6695-PK) or equivalent 1 g 2. Amberlite XAD-7 CAT # L19564 Alfa Aesar Germany 500g 3. (Diacetoxyiodo) benzene CAS [3240-34-4] Reagent Grade, Purity ≥ 98% 25 g 4. *1,1′-Bis(diphenylphosphino)ferrocene]dichloropalladium(II) CAS [72287-26-4] Purity ≥ 97% 1 g 5. 1-(trifluoromethyl)-4-vinylbenzene, CAS [402-50-6] Reagent Grade, Purity ≥ 96% 1 g 6. 1,1,1,3,3,3-Hexafluoro-2-propanol,CAS [920-66-1] Reagent Grade, Purity ≥99% 5 g 7. 1,3-Dithiane TCI Japan, Sigma-Aldrich or equivalent, Purity 99% 5 g 8. 1,4-Dioxane dried. CAS [123-91-1] SeccoSolv® - Merck 1031101001 or equivalent 2x1 L 9. 1-Methyl-4-vinylbenzene, CAS [622-97-9] Reagent Grade, Purity ≥ 98% 5 mL 10. 2-Phenyl phenol (Fungicide) 11. 2,2- Dimethyl hexanoic acid, CAS[813-72-9] Reagent Grade, Purity ≥ 97% 5 g 12. 2,2- Dimethyl succinate 13. 2,2,6,6-Tetramethylpiperidine 1-oxyl, TEMPO CAS [2564-83-2] Reagent Grade, Purity ≥ 98% 5 g 14. 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) or ABTS Purity >98%, Aldrich, Bioworld or equivalent 10 g 15. 2,6 dimethoxy phenol (DMP) 16. 2,6-Dimethoxy-1,4-benzoquinone, CAS [530-55-2] Reagent Grade, Purity 97% 5 g 17. 2,6-Di-t-butylpyridine, CAS [585-48-8] Reagent Grade, Purity ≥ 97% 1 g 18. 2,6-lutidine Reagent Grade, Purity ≥ 98% 100 mL 19. 2.6-Dimethyl phenol Purity 99% 25 g 20. 2-Chloroaniline, CAS [95-51-2] Reagent Grade, Purity ≥ 98% 250 mL 21. 2-Chlorobutane, CAS 78-86-4] Reagent Grade, Purity ≥ 98% 100 mL 22. 2-Methylthiophene TCI Japan, Sigma-Aldrich or equivalent, purity 99% 25 g 23. 2-propanol (Isopropanol) Anhydrous, 99.5%,Sigma Aldrich Cat# 278475,or equivalent. 7 L 24. 3-(Difluoromethoxy)aniline, CAS [22236-08-4] Reagent Grade, Purity ≥ 97% 1 g 25. 3-(Difluoromethyl)aniline,CAS [368-99-0] Purity ≥ 97% 1 g 26. 3'-(Methylthio)acetanilide CAS [2524-78-9] Purity ≥ 97% 5 g 27. 3'-(Trifluoromethyl)acetophenone CAS [349-76-8] Purity ≥ 98% 5 g 28. 3-(Trifluoromethyl)aniline, CAS [98-16-8] Purity ≥ 99% 5 g 29. 3,3’-Dimethoxy benzidine (o-Dianisidine) Purity 99%, Sigma/Aldrich, Scharlau, Panreac or equivalent 25g 30. 3,4-Dimethoxybenzyl alcohol ( Veratryl alcohol) Sigma/Aldrich, Scharlau, Panreac or equivalent 100 g 31. 3-Bromoaniline,CAS [591-19-5] Reagent Grade, Purity ≥ 98% 25 g 32. 3'-Chloroacetanilide CAS [587-07-8] Reagent Grade, Purity ≥ 98% 25 g 33. 3-Chloro-N-methylaniline CAS [7006-52-2] Reagent Grade, Purity ≥ 97% 5 g 34. 3-chlorophenylisocyanate CAS [2909-38-8] Reagent Grade, Purity ≥ 97% 25 g 35. 3-Fluoro-4-methoxyanilineCAS [366-99-4] Reagent Grade, Purity ≥ 98% 5 g
Transcript
Page 1: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

LIST OF CHEMICALS (A)

S. No. Description Item Specification Quantity Required

1. Amino acid mix (20 AA) "Cell free" Isotopic amino acids,chemical purity (96-98%) (Cambridge Isotope Laboratories, Inc, cat # NLM-6695-PK) or equivalent

1 g

2. Amberlite XAD-7 CAT # L19564 Alfa Aesar Germany 500g

3. (Diacetoxyiodo) benzene CAS [3240-34-4] Reagent Grade, Purity ≥ 98% 25 g

4. *1,1′-Bis(diphenylphosphino)ferrocene]dichloropalladium(II) CAS [72287-26-4] Purity ≥ 97% 1 g

5. 1-(trifluoromethyl)-4-vinylbenzene, CAS [402-50-6] Reagent Grade, Purity ≥ 96% 1 g

6. 1,1,1,3,3,3-Hexafluoro-2-propanol,CAS [920-66-1] Reagent Grade, Purity ≥99% 5 g

7. 1,3-Dithiane TCI Japan, Sigma-Aldrich or equivalent, Purity 99% 5 g

8. 1,4-Dioxane dried. CAS [123-91-1] SeccoSolv® - Merck 1031101001 or equivalent 2x1 L

9. 1-Methyl-4-vinylbenzene, CAS [622-97-9] Reagent Grade, Purity ≥ 98% 5 mL

10. 2-Phenyl phenol (Fungicide)

11. 2,2- Dimethyl hexanoic acid, CAS[813-72-9] Reagent Grade, Purity ≥ 97% 5 g

12. 2,2- Dimethyl succinate

13. 2,2,6,6-Tetramethylpiperidine 1-oxyl, TEMPO CAS [2564-83-2] Reagent Grade, Purity ≥ 98% 5 g

14. 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) or ABTS Purity >98%, Aldrich, Bioworld or equivalent 10 g

15. 2,6 dimethoxy phenol (DMP)

16. 2,6-Dimethoxy-1,4-benzoquinone, CAS [530-55-2] Reagent Grade, Purity 97% 5 g

17. 2,6-Di-t-butylpyridine, CAS [585-48-8] Reagent Grade, Purity ≥ 97% 1 g

18. 2,6-lutidine Reagent Grade, Purity ≥ 98% 100 mL

19. 2.6-Dimethyl phenol Purity 99% 25 g

20. 2-Chloroaniline, CAS [95-51-2] Reagent Grade, Purity ≥ 98% 250 mL

21. 2-Chlorobutane, CAS 78-86-4] Reagent Grade, Purity ≥ 98% 100 mL

22. 2-Methylthiophene TCI Japan, Sigma-Aldrich or equivalent, purity 99% 25 g

23. 2-propanol (Isopropanol) Anhydrous, 99.5%,Sigma Aldrich Cat# 278475,or equivalent. 7 L

24. 3-(Difluoromethoxy)aniline, CAS [22236-08-4] Reagent Grade, Purity ≥ 97% 1 g

25. 3-(Difluoromethyl)aniline,CAS [368-99-0] Purity ≥ 97% 1 g

26. 3'-(Methylthio)acetanilide CAS [2524-78-9] Purity ≥ 97% 5 g

27. 3'-(Trifluoromethyl)acetophenone CAS [349-76-8] Purity ≥ 98% 5 g

28. 3-(Trifluoromethyl)aniline, CAS [98-16-8] Purity ≥ 99% 5 g

29. 3,3’-Dimethoxy benzidine (o-Dianisidine) Purity 99%, Sigma/Aldrich, Scharlau, Panreac or equivalent 25g

30. 3,4-Dimethoxybenzyl alcohol ( Veratryl alcohol) Sigma/Aldrich, Scharlau, Panreac or equivalent 100 g

31. 3-Bromoaniline,CAS [591-19-5] Reagent Grade, Purity ≥ 98% 25 g

32. 3'-Chloroacetanilide CAS [587-07-8] Reagent Grade, Purity ≥ 98% 25 g

33. 3-Chloro-N-methylaniline CAS [7006-52-2] Reagent Grade, Purity ≥ 97% 5 g

34. 3-chlorophenylisocyanate CAS [2909-38-8] Reagent Grade, Purity ≥ 97% 25 g

35. 3-Fluoro-4-methoxyanilineCAS [366-99-4] Reagent Grade, Purity ≥ 98% 5 g

Page 2: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

36. 3-Fluoro-N-methylaniline CAS [1978-37-6] Reagent Grade, Purity ≥ 97% 1 g

37. 3-Methylthiophene TCI Japan, Sigma-Aldrich or equivalent, purity 99% 50 mL

38. 4-(1-Piperidinyl)aniline, CAS [2359-60-6] Reagent Grade, Purity ≥ 97% 5 g

39. 4-(Difluoromethoxy)aniline,CAS [22236-10-8] Reagent Grade, Purity ≥ 97% 5 g

40. 4'-(Methylsulfonyl) acetanilide CAS [22821-80-3] Reagent Grade, Purity ≥ 96% 5 g

41. 4'-(Methylsulfonyl)acetophenone CAS [10297-73-1] Reagent Grade, Purity ≥ 97% 5 g

42. 4,6-dimethyl dibenzothiophene TCI Japan, Sigma-Aldrich or equivalent, Purity 99% 25 g

43. 4-Acetamidobenzaldehyde CAS [122-85-0] Reagent Grade, Purity ≥ 97% 5 g

44. 4'-Aminoacetanilide, CAS [122-80-5] Reagent Grade, Purity ≥ 98% 25 g

45. 4-aminoantipyrine Purity 97%, Sigma – Aldrich, Biobasic or equivalent 250 g

46. 4-Chloroaniline, CAS [106-47-8] Reagent Grade, Purity ≥ 98% 25 g

47. 4'-Cyanoacetanilide, CAS [35704-19-9] Reagent Grade, Purity ≥ 98% 5 g

48. 4-Fluoro-N-methylaniline, CAS [459-59-6] Reagent Grade, Purity ≥ 97% 1 g

49. 4'-Methoxyacetanilide, CAS [51-66-1] Reagent Grade, Purity ≥ 97% 5 g

50. 4-Methoxy-N-methylaniline, CAS [5961-59-1] Reagent Grade, Purity ≥ 98% 1 g

51. 4-methyl dibenzothiophene TCI Japan, Sigma-Aldrich or equivalent, Purity 99% 25 g

52. 4-nitro blue tetrazolium chloride (NBT) Pk of 3 ml (300 mg) (analytical grade) 3 mL (300 mg)

53. 4-Nitroacetanilide, CAS [104-04-1] Reagent Grade, Purity ≥ 98% 5 g

54. 5-bromo-4-chloro-3-indolyl phosphate (BCIP) 2.31 mmol/liter (analytical grade) 1x(2.31 mmol/L)

55. Acetic acid ACS reagent, ≥99.7%, Cat# 338826, Sigma Aldrich or equivalent 5 L

56. Acetone Sigma/Aldrich, Scharlau, Panreac or equivalent (AR grade) 10 L

57. Acetone dried. (Max. 0.0075% H₂O) SeccoSolv® or equivalent 5 x 1 L

58. Acetonitrile , gradient grade for liquid chromatography HPLC grade 10 L

59. Acetonitrile dried (Max. 0.0005% H₂O) SeccoSolv® or Equivalent 1 L

60. Acetonitrile hypergrade for LC-MS LC-MS Grade , 99.9% BDH, Sigma Aldrich 20 x 2.5 L

61. Acetosyringone A-104 Phyto-USA

62. Acrylamide Molecular Biology grade, ≥99% (HPLC), Cat# A3553, Sigma or equivalent (Origin should be Europe or USA)

100 g

63. Acrylamide/Bis-acrylamide Bio Reagent, for molecular biology, 29:1 (ratio), Cat# A3574, Sigma or equivalent. Origin should be Europe or USA; Supplier should be sole agent

of the manufacturing company

100 mL

64. Aflatoxin B1 from Aspergillus flavus HPLC grade 10 mg

65. Alkali lignin Sigma/Aldrich, Scharlau, Panreac oe equivalent 2x100g

66. Aluminum chloride 99 % Oxoid or equivalent Anhydrous, 5g

67. Amino acid standards, analytical standard, acidics, neutrals, and basics HPLC and GC: suitable Storage temp. 2-8°C(Sigma-Aldrich, Cat # A9906) or equivalent

5 mL

68. Ammoium seleno cyanate Reagent Grade, Purity ≥ 97% 25 g

69. Ammonium nitrate Merck or equivalent 1 Kg

70. Ammonium sulphate Sigma/Aldrich, Scharlau, Panreac or equivalent (AR grade) 500 g

71. Ascorbic acid (AsA) Daejung, Bio Basic, Bio world or equivalent 1Kg

72. Azure B

73. Benomyl (Benlate) Fungicide

Page 3: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

74. Benzene HPLC Grade 2.5 L

75. Benzenesulfonic acid TCI Japan, Sigma-Aldrich or equivalent, purity 99% 25 g

76. Beta-mercaptoethanol or (2-Mercaptoethanol) 10 ml, 99% HPLC grade 5x10mL

77. Bis(diphenylphosphino)methane (dppm) CAS [2071-20-7] Reagent Grade, Purity ≥ 97% 2 g

78. Bis(pinacolato)diboronCAS [73183-34-3] Reagent Grade, Purity ≥ 97% 5 g

79. Bis(trichloromethyl) carbonate (triphosgene) CAS Number [32315-10-9] Reagent Grade, Purity ≥ 98% 25 g

80. Bis-acrylamide Dehydrated,white crystals or powder, purity 99%, Molecular biology grade, No DNase/Protease/ RNase, CAS # 110-26-9, BIO world or equivalent. (Origin should be Europe or USA)

2 x 100g

81. Blue dextran

82. Boric acid Purity ≥99.5%, HPLC/electrophoresis grade, Cat# B7901-500G, Sigma or equivalent.(Origin should be Europe or USA)

2 x 1Kg

83. Bromocresoul Green Merck or equivalent

84. Bromocresoul Red Merck or equivalent

85. Bromophenol blue Analytical grade, for chromatography, Cat# B0126-25G, Sigma or equivalent. 100 g

86. Bradford reagent for protein quantification Ready to use for quick measurements. Sigma or equivalent. Origin should be

Europe or USA; Supplier should be sole agent of the manufacturing company

87. n-Butanol 99% pure, HPLC grade. Origin should be Europe or USA; Supplier should be

sole agent of the manufacturing company 2 L

88. Butyldi-1-adamantylphosphine | CAS Number: [321921-71-5] Reagent Grade, Purity ≥ 95% 1 g

89. Calcium carbonate (CaCO3) Microbiological Grade, Merck/Sigma Aldrich or equivalent

90. Calcium chloride anhydrous Calcium cloride anhydrous (Daejung or equivalent brand); chemical formula CaCl2, molar mass 110.98, Assay 93%, grade value CP, pack size 1kg, density 1.101 g/cm3, boiling point 1600 °C, melting point 782 °C.

10x1 Kg

91. Calcium chloride dihydrate for molecular biology, ≥99%,Sigma Cat#C3306or equivalent. 500 g

92. Calcium hydride Reagent Grade, Purity ≥ 95% 100 g

93. Carbamide Reagent Grade, Purity ≥ 95% 100 g

94. Cesium carbonate Reagent Grade, Purity ≥ 98% 25 g

95. Cesium pivalate Reagent Grade, Purity ≥98% 5 g

96. CHAPS PUFFERAN® ≥98 %, for biochemistry, Buffer substance, zwitterionic

detergent, 3-[(3-Cholamidopropyl)dimethyl ammonio]-1-propane sulphonate. Roth (Cat No. 1479.2) or or equivalent (Origin should be Europe or USA)

25g

97. Chloroform for liquid chromatography AR/ HPLC Grade MP Bio or equivalent 5 L

98. Citric acid (Powder) AR grade 3 Kg

99. Cobalt chloride

100. Compost/Peat Mass imported 300 L/pack

101. Congo red

102. Coomaise brilliant blue R 250 Protein stain for gel electrophoresis,Cat# 20278, Thermo or equivalent. 50 g

103. Coomassie brilliant blue G250 Analytical Grade 25 g

104. Coomassie blue ready to use for quick staining of Protein gels Origin should be Europe or USA; Supplier should be sole agent of the

manufacturing company 1000 ml

105. Copper (I) Chloride Reagent Grade, Purity ≥98% 5 g

Page 4: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

106. Copper(I) Iodide Reagent Grade, Purity ≥99% 100 g

107. Crack Set Cat # 1.14687.0001, Merck or equivalent 3 Sets

108. Crystal violet powder Crystal violet powder (Daejung or equivalent brand); linear formula C25H30ClN3, molecular weight 570.12, grade GR, pack size 25G.

10x25g

109. CTAB 10g

110. Cyclophoshamide monohydrate Molecular biology grade 5 g

111. Dibenzothiophene sulfone TCI japan, Sigma-Aldrich or equivalent, Purity 99% 25 g

112. Dichloro(p-cymene)ruthenium(II) dimer CAS No. [52462-29-0] Reagent Grade, Purity ≥97% 2 g

113. Dichloroethane (DCE) Reagent Grade, Anhydrous, 99.8 % 250 mL

114. Dichloromethane HPLC/AR grade 5 L

115. Dichloromethane dried Max. 0.004% H₂O , SeccoSolv® OR Equivalent 2 x 1 L

116. Diethylene triamine pentaacetic acid (DTPA) Daejung, Bio Basic, Bio world or equivalent 500 mg

117. Diethylether Purity 99.5 %, extra dry, Sealed, Acros 364330010 or equivalent 2 x 1 L

118. Diisopropylethylamine, CAS [7087-68-5] Purity 99.5% 50 mL

119. Dimethyl itaconate, CAS [617-52-7] Reagent Grade, Purity ≥98% 5 g

120. Dimethyl sulfoxide (DMSO) Dimethyl sulfoxide (Daejung or equivalent brand); chemical formula(CH3)2SO; C2H6OS, molar mass 78.13, grade value HPLC, pack size 1L,density 1.101 g/cm3, Boiling point 189 °C, melting point 18.4 °C, flash point 95 °C, Purity ≥ 99.9 %.

10x 1 L

121. Dimethyl sulfoxide dried (Max. 0.025% H₂O) SeccoSolv® or equivalent 1L

122. Dimethylamine Reagent Grade, Purity >98% 250 mL

123. Dimethylcarbamoyl chloride, CAS [79-44-7] Reagent Grade, Purity >98% 100 g

124. Dimethylthiocarbamoyl chloride, CAS [16420-13-6] Reagent Grade, Purity >97% 25 g

125. Dimethylvinylphosphonate, [4645-32-3] Reagent Grade, Purity >96% 5 mL

126. di-Potassium hydrogen phosphate (Potassium phosphate dibasic) ACS reagent, ≥98%, Cat# P3786, Sigma Aldrich or equivalent.

127. Disodium hydrogen phosphate

For molecular biology, ≥98.5% (titration), Cat# S3264-500G, Sigma or equivalent.

128. Disodium phenyl phosphate Sigma or equivalent 10 g

129. Di-t-butyl dicarbonate, CAS [24424-99-5] Reagent Grade, Purity 99% 25 g

130. Dodecyl-beta-D-maltoside (DDM)

≥99 %, for biochemistry, Non-ionic detergent. Roth (Cat No. CN26.4) or equivalent (Origin should be Europe or USA)

10 g

131. EDTA ACS reagent, 99.4-100.6%, powder, Cat# E9884-500G, Sigma or equivalent 500 g

132. Emmersion oil RDH/BDH or equivalent 500 ml

133. Ethanol HPLC Grade 2x 2.5 L

134. Ethanol Absolute, ≥99 (pure), RDH or equivalent. Origin should be Europe or USA;

Supplier should be sole agent of the manufacturing company 10 x 2.5 L

135. Ethanol dried. (Max. 0.01 % H₂O) SeccoSolv® or equivalent 1 L

136. Ethyl acetate Ethyl acetate 99-99.9% (Daejung, BDH, Sigma-Aldrich or equivalent brand); chemical formula CH3COOC2H5, molar mass 88.11 g/mol, grade value EP, assay 99%, pack size 1L, density 0.902 g/cm3, boiling point 75~77.5 °C, density 1.62 g/cm3.

30 L

137. Ethyl acetate dried (Max. 0.003% H₂O) SeccoSolv® OR Equivalent 2 x 1 L

Page 5: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

138. Ethyl acrylate [140-88-5] Reagent Grade, Purity >99.5 % 100 mL

139. Ethyl benzoate AR grade 100 mL

140. Ethyl methane sulfonate (both liquid and powder) Daejung, Bio Basic, Bio world or equivalent 50 mL, 100 g

141. Ethylene diamine disuccinic acid (EDDS) Daejung, Bio Basic, Bio world or equivalent 1L

142. Ethylenediaminetetra acetic acid (EDTA) Daejung, Bio Basic, Bio world or equivalent 1Kg

143. Ferric chloride (FeCl3. 6HO2) Iron(III) chloride hexahydrate (Daejung, BioBasic or equivalent brand); chemical formula FeCl3.6H2O, molar mass 270.3 g/mol, assay 98%, Grade EP, pack size 1 kg, boiling point 280~285 °C, melting point 37 °C.

2 Kg

144. Fetal Bovine Serum 100 ml/cell culture grade (Invitrogen or equivalent ) 1x100 mL

145. Folin phenol Merck or equivalent 500 mL

146. Formamide ≥99.5% (GC), BioReagent, for molecular biology, Cat# F9037-100ML, Sigma or equivalent

100 mL

147. Fulvic acid Daejung, Bio Basic, Bio world or equivalent 100 g

148. Gallic acid (tannic acid) or 3,4,5- trihydroxy benzoic acid Daejung, Bio Basic, Bio world or equivalent 100 g

149. Gel Rite/Gelzan/gum 1 Kg

150. Glucose Daejung, Bio Basic, Bio world or equivalent 2x500 g

151. Glutamic acid Daejung, Bio Basic, Bio world or equivalent 500 g

152. Glutamine USP Grade Daejung, Bio Basic, Bio world or equivalent 500 g

153. Glutaraldehyde Daejung, Bio Basic, Bio world or equivalent 500 mL

154. Glutathione (GSH) Daejung, Bio Basic, Bio world or equivalent 50 g

155. Glycerol For molecular biology, ≥99%, Chem-Lab Belgium, or equivalent. 2.5 L

156. Glycerol Glycerol 85% (merck or equivalent brand); pack size 1L in plastic bottle, density .23 g/cm3 (20 °C), boiling point >30 °C (03 hPa), flash point 199 °C, melting point -10 °C, pH value 5 (100 g/l, H2O2 20 °C), vapor pressure 8 hPa (20 °C), grade value Ph Eur,BP.

10x 1L

157. Glycine For electrophoresis, ≥99%, Deajung, Korea or Roth or equivalent. 500 g

158. Glycine (2-13C, 99%; 15N, 98%+) Amino acid standard 2 box

159. Guaiacol (Liquid) Daejung, Bio Basic, Bio world or equivalent 250 g

160. Guaiacol (powder) Sigma/Aldrich, Scharlau, Panreac or equivalent 250 g

161. Guanidine hydrochloride For molecular biology, ≥99%, Cat# G3272, Sigma or equivalent. 100 g

162. HEPES PUFFERAN®

CELLPURE® ≥99,5 %, For cell culture, tissue culture and

biotechnology. DNAse, RNAse and Protease free. Cat# HN77.4 Roth or equivalent.

500 g

163. Horseradish peroxidase (salt free) 200 units/ mg (analytical grade) 1x(200U/ mg)

164. Hydrochloric acid Hydrochloric acid 37% (Scharlau, sigma or equivalent brand); chemical formula HCl, molar mass 36.46 g/mol, pack size 2.5L, density 1.19 g/cm3, solubility in water at 20 °C miscible, Boiling point 50 °C, melting point 28 °C, vapor pressure 20 °C 190 hPa.

15x2.5 L

165. Hydrochloric acid 250ML, 35% HPLC grade 2x (250 ml)

166. Hydrofluoric acid Sigma/Aldrich, Scharlau, Panreac or equivalent, AR grade 2x (200 ml)

167. Hydrogen peroxide Merck, RDH or equivalent 2 L

168. Hygromycin B powder Molecular Weight: 527.52 g/mol

Page 6: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

169. Hypoxanthine Powder, BioReagent, suitable for cell culture, ≥99%, Sigma or equivalent. 5 g

170. Imidazole For molecular biology, ≥99% (titration), Mol. Wt. 68.08, Roth or equivalent 250 g

171. Iodoacetate 100g, 98% HPLC grade 1x (100 g)

172. Isoamyl alcohol

173. Iron Phosphate Analytical Grade (Merck/Sigma Aldrich) 250 g

174. Iron sulphate Analytical Grade (Merck/Sigma Aldrich) 500 g

175. Lactic acid Daejung, Bio Basic, Bio world or equivalent 500 ml

176. L-cysteine hydrochloride hydrate L-cysteine hydrochloride hydrate (Daejung or equivalent brand); chemical formula C3H7NO2S.HCl.H2O, molar mass 175.64 g/mol, grade value GR, assay 99%, pack size 25 g.

10x25 g

177. Lithium metal Granular >99% 4 x 25 g

178. Loffer’s methylene blue Merck, RDH or equivalent 10 g

179. Magnesium chloride anhydrous Applichem, RDH, BDH or equivalent 1Kg

180. Magnesium chloride hexahydrate 99 %, p.a., ACS reagent for th molecular biology. Roth (Cat. No. 2189.1) or equivalent. Origin should be Europe or USA

1Kg

181. Magnesium metal Reagent Grade >98% 500 g

182. Magnesium sulphate 99 % Oxoid or equivalent 1 Kg

183. Malic acid Applichem, RDH, BDH or equivalent 500 g

184. Manganese sulphate Daejung, Bio Basic, Bio world or equivalent 500 g

185. Mannitol Applichem, RDH, BDH or equivalent 1Kg

186. Mercuric sulphate (HgSO4) Daejung, Bio Basic, Bio world or equivalent 500 g

187. Meta-phosphoric acid Daejung, Bio Basic, Bio world or equivalent 2.5 L

188. Methanesulfonic acid, CAS [75-75-2] Purity 98%, Alfa Aesar A13565 or equivalent 100g

189. Methanol HPLC Grade Analytical grade, Purity 99.9 % BDH, Sigma Aldrich or equivalent, Supplier

should be sole agent of the manufacturing company 7.5 L

190. Methanol hypergrade for LC-MS LC-MS Grade 10 x 2.5 L

191. Methionine Daejung, Bio Basic, Bio world or equivalent 100g

192. Methylaminoformyl chloride CAS [6452-47-7] Reagent Grade, Purity > 98% 5 g

193. Methylene blue

194. Molecular sieve 3 A 3 A or 0.3 nm 3 x 1Kg

195. m-Xylene CAS [108-38-3] Reagent Grade, Purity > 99 % 1 L

196. N,N′-Dimethylethylenediamine (DMEDA) CAS[110-70-3] Reagent Grade ,Purity > 97 % 5 mL

197. N,N-Dimethylformamide dried (Max. 0.003% H₂O) SeccoSolv® or equivalent 1L

198. n-Hexane Sigma/Aldrich, Scharlau, Panreac or equivalent, HPLC grade 10 L

199. n-Hexane n-Hexane (Daejung or equivalent brand); chemical formula C6H14, molar mass 86.18 g/mol, grade value EP, assay 95 %, pack size 5 L, density 0.659 g/cm3, boiling point 69 °C, melting point 95 °C, density 0.659 g/cm3.

10x5L

200. n-Hexane dried (Max. 0.004% H₂O) SeccoSolv® or equivalent 2 x (500 mL)

201. n-Hexdecane n-Hexdecane (Merck or equivalent brand); chemical formula CH3(CH2)14CH3, molar mass 226.44 g/mol, pack size 1L in glass bottle, density 0.77 g/cm3 (20 °C), melting point 18 °C, flash point 135 °C, vapor pressure <0.1 hPa (20 °C).

10x1L

202. Ni Sepharose 6 Fast Flow Hihg performance 25 mL

Page 7: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

203. Nile red (dye) Flourescent probe Sigma/Aldrich, Scharlau, Panreac or equivalent 100 mg

204. Nitric Acid Lab Scan/Merck or equivalent 4x2.5L

205. Nitroblue tetrazolium Daejung, Bio Basic, Bio world or equivalent 500g

206. N-Methyl-p-toluidine CAS [623-08-5] Reagent Grade, Purity - 98 % 25 mL

207. Nystatin Sigma/Aldrich, Biobasic or equivalent 5MU

208. o-Anisidine CAS [90-04-2] Reagent Grade, Purity >98% 5 g

209. o-Chloranil, CAS [2435-53-2] Reagent Grade, Purity ≥99% 5 g

210. Oxalic acid Daejung, Bio Basic, Bio world or equivalent 500 g

211. Palladium(II) acetylacetonate CAS [14024-61-4] Reagent Grade ,Purity ≥99% 2 g

212. p-anisidine CAS [104-94-9] Reagent Grade, Purity ≥99% 5 g

213. p-Chloranil, CAS [118-75-2] Reagent Grade, Purity ≥99% 5 g

214. Perchloric acid 500ml, 70% HPLC grade 1x 500 mL

215. Petroleum Ether Lab Scan, RDH or equivalent 5x2.5 L

216. Phenol 98-99% pure HPLC 2x 01Kg

217. Phenol red

218. Phosphoric acid Lab Scan, RDH or equivalent 2.5 L

219. PMSF High Purity Grade ≥99%, Cat# P7626-5G, sigma or equivalent. 5 g

220. p-nitrophenyl- β-D-cellobioside (pNPC)

221. Potassium antimony tartrate Daejung, Bio Basic, Bio world or equivalent 250g

222. Potassium bicarbonate, CAS [298-14-6] Purity ≥99% 100 g

223. Potassium carbonate anhydrous, powder, BioXtra, ≥99.0% Cat# P5833-1Kg, sigma or equivalent 1 Kg

224. Potassium chloride powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99.0%, Cat# P5405-250G, sigma or equivalent

1Kg

225. Potassium dichromate (K2Cr2O7) Merck, RDH, Daejung, Bio Basic, Bio world or equivalent 3x1Kg

226. Potassium dihydrogen phosphate 99 % oxoid or equivalent 1 Kg

227. Potassium di-hydrogen phosphate

Potassium dihydrogen phosphate crystals (KH2PO4) Merck or equivalent brand; grade value Ph Eur, BP, JPC, NF,E 340; chemical formula KH2PO4, molar mass 136.08 g/mol, pack size 5 kg in plastic bottle, density 2.33 g/cm3 (21.5 °C), melting point 253 °C (decomposition), pH value 4.2 - 4.6 (20 g/l, H₂O, 20 °C), solubility 208 g/l.

228. Potassium di-hydrogen phosphate (Potassium phosphate monobasic) Powder, ≥98 %, Ph.Eur., USP, BP, Cat# P018.1, Roth or equivalent. (Origin should be Europe or USA)

229. Potassium ferricyanide K3[Fe(CN)6] Sigma-Aldrich,Merck, RDH, Daejung, Bio Basic, Bio world or equivalent

230. Potassium hydoxide

231. Potassium hydrogen pathalate (KHP) Daejung, Bio Basic, Bio world or equivalent 500g

232. Potassium iodide Purity 99.5% 1x50g

233. Potassium nitrate Microbiological Grade, Merck/Sigma Aldrich or equivalent

234. Potassium permanganese Daejung, Bio Basic, Bio world or equivalent 500 g

235. Potassium phosphate dibasic anhydrous ≥98 %, Ph.Eur., BP, anhydrous, Cat# T875.1, Roth or equivalent. (Origin should be Europe or USA)

236. Potassium sodium tartrate

237. Potassium tert-butoxide, CAS [865-47-7] Reagent grade, Purity ≥98% 5g

Page 8: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

238. Potassium triphosphate, CAS [7778-53-2] 97% pure, anhydrous 25g

239. p-Toluenesulfonamide, CAS [70-55-3] Reagent Grade, Purity ≥ 98% 250 g

240. p-Toluenesulfonanilide, CAS [68-34-8] Reagent Grade, Purity ≥ 98% 25 g

241. p-Xylene p-Xylene (Merck or equivalent brand); Chemical formula C6H4-1, 4-(CH3)2, molar mass 106.17 g/mol, Grade ISO, pack size 2.5 L in glass bottle, boiling point 138.4 °C (1013 hPa), density 0.86 g/cm3 (20 °C), melting point 13.3 °C, vapor pressure 8.7 hPa (20 °C), solubility 0.2 °g/l.

10x2.5 L

242. p-xylene, CAS [106-42-3] Reagent Grade, Purity ≥ 99% 100 mL

243. Pyridine (for GC) ACS grade, >99.0 % pure 500 mL

244. Pyridine dried (Max. 0.0075 % H₂O) SeccoSolv® or equivalent (1L)

245. Resazurin, sodium salt Resazurin, sodium salt (sigma or equivalent brand); dye content 80%, pH range 3.8 - 6.5, orange to violet, water soluble, absorbtion 600 nm, pack size 5g in glass bottle, empirical formula C12H6NNaO4, molecular weight 251.17.

10 x5 g

246. Resorcinol Daejung, Bio Basic, Bio world or equivalent 50g

247. Riboflavin Daejung, Bio Basic, Bio world or equivalent 100g

248. Rifampicin USP grade Daejung, Bio Basic, Bio world or equivalent 25g

249. Ruthenium(III) chloride Reagent Grade, Purity ≥ 98% 2 g

250. Safe-green (stain for nucleic acid) Stain (dye) for nucleic acid binding while analysis on agarose and polyacrylamide gel. Can be detected by blue and UV light. (Origin of manufacturer should be Europe, USA and Canada)

1m L x 10

251. Salicyclic acid Daejung, Bio Basic, Bio world or equivalent 500g

252. Sea sand purified by acid and calcined for analysis. CAS 14808-60-7 - (For Liquid Chromatography) 1 Kg

253. Selenium Powder/ Metal Merck/ RDH or equivalent 100g

254. Selenourea CAS 630-10-4 Purity 99% 5g

255. Silica Gel 60 for column chromatography, CAS No. 7631-86-9 0.04-0.063mm, 230-400 Mesh ASTM, Merck® or equivalent 1Kg

256. Silicic acid Daejung, Bio Basic, Bio world or equivalent 500g

257. Silver nitrate, CAS [7761-88-8] Reagent Grade, Purity 99% 5g

258. Silver sulphate (AgSO4) Daejung, Bio Basic, Bio world or equivalent 500g

259. Sodium acetate ≥99 %, extra pure, anhydrous, Cat# X891.2, Roth or equivalent 4x250g

260. Sodium ammonium hydrogen phosphate Daejung, Bio Basic, Bio world or equivalent 1 Kg

261. Sodium azide (NaN3) ≥99%, ultra dry, Cat# 769320-100G, Sigma or equivalent. HPLC grade 2 x (50g) 500g

262. Sodium bicarbonate or Sodium hydrogen carbonate Sodium hydrogen carbonate ≥99%, (Merck, Roth or equivalent brand); chemical formula NaHCO3, molar mass 84.01g/mol, pack size 1 kg in plastic bottle, density 2.22 g/cm

3 (20 °C), melting point 270 °C (decomposition), pH

value 8.6 (50 g/l, H2O, 20 °C), solubility 96 g/l.

12x1 Kg

263. Sodium carbonate, anhydrous CAS [497-19-8] Sodium carbonate anhydrous Purity 99% (Merck or equivalent brand); Chemical formula Na2CO3, molar mass 105.99 g/mol, density 2.53 g/cm

3 (20

°C), melting point 854 °C, pH value 11.16 (4 g/l, H2O, 25 °C), solubility 212.5 g/l, pack size 1 kg in plastic bottle, bulk density 1100 kg/m

3.

15 x1Kg

264. Sodium chloride (NaCl) Sodium chloride ≥99 %, (Merck, Roth or equivalent brand); Chemical formula NaCl, molar mass 58.44 g/mol, grade value ACS,ISO,Reag. Ph Eur, pack size 1 kg in plastic bottle, boiling point 1461 °C (1013 hPa), melting point 801 °C,

12x1 Kg

Page 9: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

bulk density 1140 kg/m3, density 2.17 g/cm3 (20 °C), pH value 4.5-7.0 (100 g/l, H2O, 20 °C), vapor pressure 1.3 hPa (865 °C).

265. Sodium chlorite

266. Sodium dodecyl sulfate Sodium dodecyl sulfate (Merck or equivalent brand); empirical formula CH3(CH2)11SO4Na, pack size 1 kg in plastic bottle, form fine white to offwhite powder, DNAse none detected, RNAse none detected, Protease none detected, Phosphatase none detected.

10x1 Kg

267. Sodium hydroxide (NaOH) 1.0 M, HPLC grade Supplier should be sole agent of the manufacturing

company 5x100 mL

268. Sodium hydroxide (NaOH) Sodium hydroxide pellet (Merck or equivalent brand); chemical formula NaOH, molar mass 40.00 g/mol, Grade ISO, density 2.13 g/cm3 (20 °C), melting point 319 - 322 °C, pH value >14 (100 g/l, H2O, 20 °C), solubility 1090 g/l, pack size 1kg. Supplier should be sole agent of the manufacturing

company

12x1Kg

269. Sodium iodide Bio Basic, Bio world, Applichem or equivalent 500 g

270. Sodium malonate Bio Basic, Bio world, Applichem or equivalent 500g

271. Sodium nitrate Bio Basic, Bio world or equivalent 2 x 500 g

272. Sodium phosphate dibasic dehydrate (Na2HPO4) ≥99 %, p.a., ACS, anhydrous, Cat# P030.1, Roth or equivalent. (Origin should be Europe or USA)

2 x 250g

273. Sodium sulphate, CAS [7757-82-6] ACS Reagent, Anhydrous, Purity 99% 500g

274. Sodium tartrate

275. Sodium tetraborate Bio Basic, Bio world or equivalent Kg

276. Spirit (methylated) Commercial grade 5L

277. Spirit (white) Clear, without smell

278. Sulphuric acid Sulfuric acid, 95-97% (Scharlau, Lab Scan/Merck or equivalent brand); chemical formula H2SO4, molar mass 98.08 g/mol, pack size 1L, density 1.84 g/cm3, solubility in water at 20 °C miscible, Boiling point 310 °C, melting point 15 °C, vapor pressure 20 °C 0.0001 hPa.

30x1L

279. SylPhos, CAS [109313-83-9] Reagent Grade, Purity ≥ 97% 500

280. Syrindaldazine

281. Tannin Daejung, Bio Basic, Bio world or equivalent 500 g

282. Tartaric acid (powder) Daejung, Bio Basic, Bio world or equivalent 500 g

283. t-Butanol Purity ≥ 99%, HPLC Grade 500 mL

284. tert-Amyl alcohol Reagent Grade, Purity ≥ 99% 250 mL

285. tert-Butyl carbamate Reagent Grade,Purity ≥ 98% 5 g

286. tert-ButylperoxybenzoateCAS [614-45-9] Reagent Grade,Purity ≥ 98% 5 g

287. Tetrabutylammonium bisulfate CAS [32503-27-8] Reagent Grade,Purity ≥ 99% 25 g

288. Tetracycline hydrochloride ≥900 µg/mg, for biochemistry and molecular biology, Bacteriostatic antibiotic, Cat# 0237.2, Roth or equivalent.

50 g

289. Tetrahydrofuran dried (Max. 0.005 % H₂O) SeccoSolv®. or equivalent 5 x 1 L

290. Tetramethylethylenediamine (TEMED) Analytical grade, 99% (GC) 3 x 100mL

291. Thiazole, CAS [288-47-1] Reagent Grade, Purity > 99% 5 g

292. Thioacetanilide, CAS [637-53-6] Reagent Grade, Purity > 98% 5 g

Page 10: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

293. Thiobarbituric acid Daejung, Bio Basic, Bio world or equivalent 25g

294. Thiophene Sigma-Aldrich or equivalent Purity 99% 100 mL

295. Thiophene 2-acetic acid Sigma-Aldrich or equivalent Purity 99% 40g

296. Thiophene 2-carboxylic acid Sigma-Aldrich or equivalent Purity 99% 50g

297. Thioxanthen-9-one Sigma-Aldrich or equivalent Purity 99% 10g

298. Thymol Sigma-Aldrich or equivalent Purity 99% 100 mL

299. Toluene HPLC Grade, Sigma-Aldrich or equivalent 2.5L

300. Toluene dried (Max. 0.005% H₂O) SeccoSolv® or equivalent 1 L

301. Trisodium citrate ABI, Roche, Amersham, Roth, Phyto, BioLab or equivalent 1Kg

302. Tris(dibenzylideneacetone)dipalladium(0) CAS [51364-51-3] Purity > 99%

303. Tris(1,1,1,3,3,3-hexafluoropropan-2-yl) phosphate CAS [66470-81-3] Reagent Grade, Purity > 98%

304. Tributylammonium acetate CAS [10534-59-5] Reagent Grade, Purity ≥ 97% 5 g

305. Tricalcium phosphate RDH/BDH or equivalent I Kg

306. Trichloro acetic acid ACS reagent, ≥99.7%, Cat# 338826, Sigma Aldrich or equivalent

307. Trichloro acetic acid Trichloroacetic acid (Daejung or equivalent brand); chemical formula C2HCl3O2, molar mass 163.39 g/mol, grade value EP, assay 99%, pack size 500 g, density g/cm

3, melting point 54~58 °C, boiling point 196, density 1.62

g/cm3.

10x500 g

308. Trifluoromethylbenzene CAS [98-08-8] Reagent Grade, Purity > 99% 25 g

309. Triphenyl formazan Sigma or equivalent 10g

310. Triphenyl tetrazolium chloride Biobasic or equivalent 10 g

311. Triton X-100 10% in H2O, Aanalytical grade 10x100ml

312. Tween 20 & Tween 80 Tween 20 & Tween 80 (Merck or equivalent brand) or Polyoxyethylene (20) Monolaurate; Pack size 1L in plastic bottle, DNAse: none detected, Heavy metals (as Pb): ≤0.001%, Hydroxyl number: 96-108, Residue after ignition: ≤0.25%, Moisture (H2O): ≤3.0%, Protease: none detected, RNAse: none detected, form Yellow to amber viscous liquid (Tween 20) and Golden yellow viscous liquid (Tween 80).

50 mL

313. Uranyle acetate Daejung, Bio Basic, Bio world or equivalent 10 mL

314. Urea

≥99.5 %, p.a., BioScience-Grade, Cat# 2317.1, Roth or equivalent. (Origin should be Europe or USA)

1Kg

315. Vinylsulfonylbenzene, [5535-48-8] Reagent Grade, Purity > 99%

316. Xylan

317. Zinc chloride 10g, 99.99% (analytical grade) 10x100 g

318. Zinc chloride (ZnCl2) ≥97%, p.a. Analytical reagent; starting material for the preparation of zinc iodine chloride solution. Cat# T887.1, Roth or equivalent.

500 g

319. Zinc sulphate ( ZnSO4.7H2O)

Zinc sulphate (Merck or equivalent brand); chemical formula ZnSO4.7 H2O, molar mass 287.54 g/mol, density 1.97 g/cm3 (20 °C), melting point 100 °C, pH value 4 - 6 (50 g/l, H2O, 20 °C), solubility 965 g/l, pack size 1 kg in plastic bottle.

10x1Kg

Page 11: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

320. Safe-Green (nucleic acid dye) Safe-Green (6x loading dye) is a stain for visualization of nucleic acid in agarose and polyacrylamide gel electrophoresis. This dye eliminates need for toxic Ethidium Bromide (a potent mutagen). Safe-Green is non-carcinogenic. It is mixed directly with samples before gel loading. Inter tracking dye is included to monitor gel progress. Results can be viewed as with EtBr staining. Safe-Green can be excited with blue or UV light, and has maximum emission at 525 nm. It detects as little as 0.2-0.6 ng of DNA per gel band.

20 vials

Page 12: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

LIST OF ENZYMES / MOLECULAR BIOLOGY/SERVICES (B)

S.No. Description Item Specification Quantity Required

1. (AMV)Reverse Treanscriptase Genetically modified full length first strand cDNA up to 13kb 5x50,000 U

2. 100bp DNA ladder Thermo Scientific, Invitrogen or equivalent (Origin should be Europe or USA;

Supplier should be sole agent of the manufacturing company) 100 applications

3. 6X Loading dye Fermentas or equivalent (Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company)

4. Amphotericin B Invitrogen or equivalent 100 mL/pack

5. Ampicillin Fermentas or equivalent (Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company)

6. Antibiotic Sensitivity Discs Oxoid or equivalent 50 discs/vial

7. AscI Fermentas or equivalent (Origin should be Europe or USA; Supplier should be sole

agent of the manufacturing company) 5000 U

8. BamHI Fermentas or equivalent. (Origin should be Europe or USA; Supplier should be sole

agent of the manufacturing company) 1 x 4000 U

9. BglII Fermentas or equivalent (Origin should be Europe or USA; Supplier should be sole

agent of the manufacturing company) 5000 U

10. Bsh1236I (BstUI) Enzyme Thermofischer or equivalent (Origin should be Europe or USA; Supplier should be

sole agent of the manufacturing company)

500 Units

11. Buffered Peptone Water Oxoid or equivalent 500 g/unit

12. Catalase Daejung or Bio Basic or Bio world or equivalent 500 mg

13. Collagenase-II enzyme Invitrogen or equivalent 5 g/pack

14. Chloroamphenicol ≥98,5 %, Ph. Eur., for biochemistry, Bacteriostatic antibiotic, Roth or equivalent 100 g

15. DNA Gel Loading Dye ThermoFisher Scientific or equivalent 5x1.25 ml

16. DNA ladder 1 Kb 1 kb DNA, range 250 to 10,000bp, Fermentas or equivalent(Origin should be

Europe or USA; Supplier should be sole agent of the manufacturing company) 1(5 x 50 µg)

17. DNA Ladder 50 bp Fermentas-Thermo, or equivalent

18. DNase I – Rnase free EN0521/ Thermo Scientific or equivalent

19. dNTP Set, 100 mM Solution Fermentas-Thermo or equivalent. (Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company)

1x(4x0.25 ml)

20. DpnI Unit activity 10 U/µL, Fermentas or equivalent. (Origin should be Europe or USA;

Supplier should be sole agent of the manufacturing company) 1000 Units

21. Dream Taq DNA Polymerase Fermentas, Thermofisher or equivalent. (Origin should be Europe or USA; Supplier

should be sole agent of the manufacturing company) 01

22. Dream Taq Green PCR Master mix (2X) Thermo Scientific, Invitrogen or equivalent (Origin should be Europe or USA;

Supplier should be sole agent of the manufacturing company) 1000 runs

Page 13: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

23. DreamTaq PCR Master Mix (2X) Fermentas or equivalent. (Origin should be Europe or USA; Supplier should be sole

agent of the manufacturing company) 1x1000 reactions

24. Eco471(AvaII) Enzyme CpG methylation-sensitive, ThermoFischer or equivalent. (Origin should be Europe

or USA; Supplier should be sole agent of the manufacturing company)

800 Units

25. EcoRI Fermentas-Thermo or equivalent. (Origin should be Europe or USA; Supplier should

be sole agent of the manufacturing company) 1x5000 U

26. EcoRV Fermentas or equivalent (Origin should be Europe or USA; Supplier should be sole

agent of the manufacturing company) 1 vial

27. Ethidium Bromide For molecular biology, powder, Sigma, Thermofishe, MP Bio or equivalent 10 ml

28. Fast Digest Restriction Enzymes: Haelll (BsuRl), EcoRl, Hindlll, BamHl, Ncol, Smal, Mfel (Munl), Bglll, Ddel (HpyF3l), Kpn2l (Acclll), Dral

ABI, Thermo Fermentas, Amersham or equivalent (Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company)

200 Runs Each

29. FastAP Thermo sensitive Alkaline Phosphatase, High efficiency enzyme with unit activity 1U/µL Cat# EF0651, Fermentas-Thermo or

equivalent. (Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company)

1x1000U

30. Gateway® LR Clonase® II Enzyme mix 20 reactions/ Gateway® LR Clonase™ II Enzyme Mix, Proteinase K Solution (2 µg/µl),

Invitrogen or equivalent 1

31. Gel and PCR clean up system Silica-membrane-based purification of PCR products >100 bp. DNA of up to 10 kb,

Promega, Fermentas or equival. (Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company)

10x250 preps

32.

Genomic DNA Purification Kit For rapid and efficient purification of high quality genomic DNA from various mammalian cell culture and tissue samples, whole blood, bacteria and yeast Thermo or equivalent, (Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company)

5 x 100 preps

33. Glucose Oxidase 200 U/mg, from Aspergillus niger, lyophilized, powder 5x (200U/mg)

34. Hin1II (NlaIII) Enzyme

Best activity at 37oC, No methylation sensitivity, sensitive to heat inactivation

Thermofischer or equivalent. (Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company)

300 Units

35. HindIII Fermentas-Thermo or equivalent. (Origin should be Europe or USA; Supplier should

be sole agent of the manufacturing company) 1x5000U

36. Horseradish peroxidase (salt free) 200 units / mg (analytical grade) 1x(200 U / mg)

37. HpaI Fermentas or equivalent. (Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company)

5 x 500 units

38. HpyF3I (DdeI) Enzyme Thermofischer or equivalent (Origin should be Europe or USA; Supplier should be

sole agent of the manufacturing company) 500 Units

39. Kanamycin sulfate Kanamycin sulfate from Streptomyces kanamyceticus powder, BioReagent, suitable

for cell culture, suitable for plant cell culture, Cat# K1377, SIGMA or equivalent.

40. KOD Hot start DNA polymerase High fidelity DNA polymerase designed for accurate PCR amplification of long strand

and GC- rich DNA templates for cloning, Cat# 71086-4, Merck Millipore. (Origin should be Europe or USA)

1x1000U

Page 14: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

41. KpnI Thermo Scientific or equivalent (Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company)

42. Lipase (Lipolase enzyme) Fermentas or equivalent (Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company)

5 x 100 units

43. Lysozyme Fermentas or equivalent. (Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company)

5g

44. Lyticase from Arthrobacterleteus Lyophilized powder, ≥2,000 units/mg protein, Protein ≥20 % by biuret, Cat# L2524,

SIGMA or equivalent. (Origin should be Europe or USA) 50 KU

45. Maxima™ SYBR Green/ROX qPCR Master Mix Thermo Scientific, Invitrogen or equivalent 200 runs

46. Monoclonal anti-poly Histidine antibody Sigma or equivalent 0.2 ml

47. MspI (HpaII) Enzyme

Best activity at 37˚C, No methylation sensitivity, (Thermo Fischer, Cat # ER0541) or equivalent

3000 Units

48. MssI (PmeI) Restriction enzyme with unit activity 5 U/µL, Cat# ER1314, Fermentas-Thermo or

equivalent. (Origin should be Europe or USA) 1x250 U

49. MvaI (BstNI) Enzyme

Best activity at 37o

C, No methylation sensitivity, sensitive to heat inactivation (Thermo-fischer, Cat # ER0551) or Equivalent

2000 Units

50. NcoI Unit activity 10 U/µL, Fermentas-Thermo or equivalent. (Origin should be Europe or

USA; Supplier should be sole agent of the manufacturing company) 1x500 U

51. NdeI Unit activity 10 U/µL, Fermentas-Thermo or equivalent. (Origin should be Europe

or USA; Supplier should be sole agent of the manufacturing company) 1x500 U

52. NheI Fermentas or equivalent (Origin should be Europe or USA; Supplier should be sole

agent of the manufacturing company) 1 vial

53. NotI Unit activity 10 U/µL, Fermentas-Thermo or equivalent. (Origin should be Europe

or USA; Supplier should be sole agent of the manufacturing company) 1x300 U

54. PacI Unit activity 10 U/µL, Fermentas-Thermo or equivalent. (Origin should be Europe

or USA; Supplier should be sole agent of the manufacturing company) 1x250 U

55. Penicillin-Streptomycin 100X Invitrogen or equivalent 100 mL/pack

56. pfu DNA Polymerase ABI, Thermo Fermentas or equivalent Origin should be Europe or USA; Supplier

should be sole agent of the manufacturing company) 2 x 100 Units

57. Pre-stained Protein Ladder Thermo Scientific, Invitrogen or equivalent (Origin should be Europe or USA;

Supplier should be sole agent of the manufacturing company) 100 applications

58. Primers/ Random Primers

Custom DNA Oligos (Primers) (Synthesized at 50 nmol scale), purified and salt free. Thermo Scientific or equivalent

2000

59. Protease (Subtilisin enzyme) Thermo-fermentas or equivalent (Origin should be Europe or USA; Supplier should

be sole agent of the manufacturing company) 5 x500 units

60. Proteinase K Proteinase K , Bio World USA or equivalent(Origin should be Europe or USA;

Supplier should be sole agent of the manufacturing company) 100 mg

61. Reverse Transcriptase Thermo Scientific or equivalent 10000 U/ vial

62. RiboRuler High Range RNA Ladder Ready to use, Thermo scientific Cat # SM1823 or equivalent 3

Page 15: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

63. RNase Inhibitor (Ribolock)

40u/µL, Inhibit eukaryotic RNases, including RNase A, RNase B and RNase C, Rapid and complete protection of RNA, Inhibited RNases at temperature up to 55°C, Active within the pH 5-9 range, Free of DNases, RNases and phosphatases Compatible with all molecular biology procedures, Thermo Scientific or equivalent

2500 U/vial)

64. Roti® Phenol/Chloroform/Isoamyl Alcohol ROTH- or equivalent origin should be Europe or USA 2 x 1000 mL

65. SacI (10U/µl Thermo Scientific or equivalent (Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company)

5 x 500 units

66. Salmonella Shigella Agar Oxoid or equivalent 500 g/unit

67. Sample Loading Solution , for Beckman Coulter CEQ 8000 6 ml

68. Separation buffer, for Beckman coulter CEQ 8000

69. Sequencing

Standard nucleotide sequencing (read length 900 to 1100 bases), Template purified

plasmid or PCR DNA, Generic primers will be provided by the service provider,

Template specific primers will be provided by the customer.

>1000 reactions

70. SmaI Unit activity 10 U/µL, Fermentas or equivalent. (Origin should be Europe or USA;

Supplier should be sole agent of the manufacturing company) 1x1200U

71. Spearation Gel-LPA1 10ml

72. Syber Green Thermo or equivalent 200 vials

73. T4 DNA Ligase Unit activity 5 U/µL, Invitrogen, Fermentas-Thermo or equivalent. (Origin should be

Europe or USA; Supplier should be sole agent of the manufacturing company) 1x250U

74. T4 DNA polymerase Thermo Scientific or equivalent (Origin should be Europe or USA; Supplier should

be sole agent of the manufacturing company) 5 x 1000 units

75. Taq DNA polymerase Thermoscientific or equivalent. (Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company)

5 x 500 units

76. Taq DNA Polymerase (recombinant) Thermo scientific or equivalent. (Origin should be Europe or USA; Supplier should

be sole agent of the manufacturing company) 1x500U

77. TaqI Enzyme Best activity at 65

oC, No methylation sensitivity, Not sensitive to heat inactivation

(ThermoFischer, Cat #ER0671) or equivalent 3000 Units

78. TaqMan Probes for SNP genotyping rs4340, rs699, rs5186, Flourophorelabelled,Functionally tested, Specific for human

genome, (Applied Biosystem, Cat # 4351379) or equivalent Small: 300 reactions

79. TaqMan Universal Master Mix II ROX (pre-mixed), Ultra pure quality, for q-PCR. (Applied Biosystem, Cat #4440047)

or equivalent 2X5 ml

80. Tri Reagent MRC or equivalent

81. Tris Base Ultra pure grade Primary Standard and Buffer, ≥99.9% (titration), crystalline, pH:

10.5-12, Cat# T1503-500G, sigma or equivalent

82. Tris HCl PUFFERAN

® ≥99 %, p.a. Ideal for all molecular biological applications, Roth or

equivalent

83. Trypsin-EDTA (0.25%) Invitrogen or equivalent 100 mL/pack

Page 16: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

84. Unstained Protein Ladder (10-200kDa Wide Range Protein) for use as molecular weight standards in SDS-

polyacrylamide gel electrophoresis, Thermo or equivalent. (Origin should be Europe or USA)

2x250 uL

85. Water, Nuclease free DNase-Free, Endonuclease-Free, Exonuclease-Free, Genomic DNA-Free, Molecular

Biology Grade, RNase-Free(Applied Biosystem, cat # R0582, Caisson Lab USA or equivalent

86. X-gal Bio Basic INC. Canada Cat # BB0083

87. XhoI Unit activity 10 U/µL, Fermentas-Thermo or equivalent. (Origin should be Europe

or USA; Supplier should be sole agent of the manufacturing company) 1x2000 U

Page 17: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

LIST OF MEDIA CHEMICALS AND MEDIA (C) S.No. Description Item Specification Quantity Required

1. Agar/ Bacteriological agar

Bacteriological Agar, Gel Strength: 550–950 g/cm2 Gelling Temperature: 34–38 °C Melting Temperature: 84–88 °C Soluble in boiling water Bio-world USA Cat No.30620001/ Sigma Eldrich, Oxide, BioBasic or equivalent ( Supplier should be sole agent of the manufacturing company)

20 x Kg

2.

Agar powder Agar-agar (merck or equivalent brand); granulated, purified and free from inhibitors for microbiology, pH value 6.8 (100 g/l, H2O2 20 °C), solubility 20 g/l slightly soluble, pack size 1 kg in plastic bottle, melting point 90 °C, bulk density 550 kg/m3, appearance brownish-yellow.

20x1Kg

3.

Agarose Agarose, molecular biology grad, High purity for preparative and analytical electrophoresis of nucleic acids. Range (200bp to 40kb) Gelling Temp.: 35-37°C* Sulfate: DNase/RNase: None detected, Roth or equivalent. (Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company)

500 g

4. Agarose low melt

Low melting and gelling temperature, Particularly for gel elution and in-gel- application in a wide size range (500 bp-20kb). Roth or equivalent. (Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company)

100 g

5. Bacto peptone For microbiology, Merck or equivalent (Supplier should be sole agent of the

manufacturing company) 1 Kg

6. Bacto soytone Difco or equivalent, enzymatic digest of soy (Supplier should be sole agent of the

manufacturing company) 10x500 g

7. Beef Extract Microbiological Grade, Merck/Sigma Aldrich or equivalent (Supplier should be sole agent of the manufacturing company)

1 Kg

8. BHI agar For microbiology, for the growth of fastidious microorganisms, pH: 7.4±0.2 (25 °C),

sigma or equivalent (Origin should be Europe or USA) 500 g

9. BHI broth For microbiology, for the growth of fastidious microorganisms, pH: 7.4±0.2 (25 °C),

sigma or equivalent (Origin should be Europe or USA) 500 g

10. Blood agar base For microbiology, sigma or equivalent (Origin should be Europe or USA) 500 g

11. Bovine Serum Albumin Lyophilized powder, ≥96% (agarose gel electrophoresis), Sigma or equivalent. 50 g

12.

BSM agar BSM agar with BSM supplement (sigma or equivalent brand), bifidus selective and differential media agar with BSM supplement; grade microbiology, final pH 6.8±0.2 (25 °C), pack size 500g in plstic bottle. BSM should contain Peptone, Meat extract, Yeast extract, Dextrose, Sodium chloride. It should contain compound in low concentration for detoxify metabolic by-products, reducing and buffering agents, selective salts and antibiotics to inhibit the growth of other microorgaanisms. Bifidobacteria should give pink-purple colonies on agar media.

20x500g

13. BSM Broth BSM broth with BSM supplement (sigma or equivalent brand), bifidus selective and

differential media broth with supplement; grade microbiology, final pH 6.8±0.2 (25

20x500g

Page 18: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

°C), pack size 500g in plstic bottle. BSM should contains Peptone, Meat extract, Yeast extract, Dextrose and Sodium chloride There should be a compound in low concentration for detoxify metabolic by-products. The medium contains reducing and buffering agents, Selective salts, antibiotics and different compounds to inhibit the growth of other microorganisms.

14. Cary Blair transport medium Oxoid or equivalent 500 g/unit

15. Casein Hydrolysate (acid hydrolyzed) for microbiology, pH 4.7 - 5.7 (50 g/l, H₂O, 20 °C).

Merck Cat# 1022450500-or equivalent. (Origin should be Europe or USA) 500 g

16. Corn Starch Microbiological Grade, Merck/Sigma Aldrich or equivalent 2Kg

17. Chromocult Coliform Agar Merck or equivalent 1Kg

18. CS Supplements Oxoid or equivalent 10/box

19. Dextrose (D-Glucose) anhydrous

Anhydrous, 96%, Microbiological Grade, Merck/Sigma Aldrich or equivalent (Supplier should be sole agent of the manufacturing company)

500 g

20. D-Fructose-6-phosphate, disodium salt

D-Fructose-6-phosphate, disodium salt (Merck or equivalent brand); form white solid, purity ≥95% by chromatography, pack size 1 gm in plastic ampoule. The storage temperature should be at 2-8°C during shipment. Must be delivered in sealed Styrofoam box with ice packs.

10x1g

21. DMEM media with Glutamine and high glucose 500ml/high glucose, L-Glutamine, Phenol Red and sodium Biocarbonate(Invitrogen

or equivalent ) 1x500ml

22. Fetal Bovine Serum Invitrogen or equivalent 100ml/pack

23. Fructose Microbiological Grade, Merck/Sigma Aldrich or equivalent 500g

24. Lactose Microbiological Grade, Merck/Sigma Aldrich or equivalent 1Kg

25. LB Broth

LB broth (Merck or equivalent brand); pack size 500 g in plastic bottle, pH valve 6.8 - 7.2 (H2O 25 °C) (after autoclaving), appearance yellowish brown, pack size 500g. The media should contain Yeast extract 5.0 g/L, peptone from casein 10.0g/L, sodium chloride 10.0 g/L. (Supplier should be sole agent of the manufacturing company)

30x500g

26.

M17 broth media M17 broth media (oxide or equivalent brand); media should consist of tryptone 5g/L, soya peptone 5 g/L, Lab Lemco powder 5 g/L, yeast extract 2.5 g/L, ascorbic acid 0.5 g/L, magnesium sulfate 0.25 g/L, Di-sodium-glycerophosphate 19 g/L, pH 6.9 ± 0.2 @ 25°C. Appearance dehydrated medium Straw colored, free-flowing powder and Prepared medium Straw colored solution, pack size 500gm in plastic bottle.

20x500g

27. MacConkey Agar

MacConkey agar (Merck or equivalent brand); pH value 6.9 - 7.3 (50 g/l, H2O, 25 °C) (after autoclaving), solubility 50 g/l, bulk density 720 kg/m3, pack size 500 g in plastic bottle. The composition (g/L) of the media should be Peptone from gelatine 17.0; Peptone from casein 1.5; Peptone from meat 1.5; Sodium chloride 5.0; Lactose 10.0, Bile salt mixture 1.5; Neutral red 0.03; Crystal violet 0.001; Agar-Agar 13.5.

25 x 500g

Page 19: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

28. Malt Extract Merck / sigma Aldrich or equivalent. (Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company)

1 Kg

29. Maltose Microbiological Grade, Merck/Sigma Aldrich or equivalent (Supplier should be sole agent of the manufacturing company)

500 g

30. MRS broth

MRS broth (Merck or equivalent brand), pH value 5.5 - 5.9 (50 g/l, H2O, 30 °C) (after autoclaving), solubility 50 g/l, pack size 500 g in plastic bottle, bulk density 620 kg/m3. The composition of the media (g/L) should be Peptone from casein 10.0; meat extract 8.0; yeast extract 4.0; D(+)-glucose 20.0; dipotassium hydrogen phosphate 2.0; Tween® 80 1.0; di-ammonium hydrogen citrate 2.0; sodium acetate 5.0; magnesium sulfate 0.2; manganese sulfate 0.04. ( Supplier should be sole agent of the manufacturing company)

60x500g

31. Mueller Hinton agar Dehydrated, Microbiology grade Oxoid or equivalent 500 g/unit

32. Nutrient agar Merck or equivalent (Supplier should be sole agent of the manufacturing company) 2 Kg

33.

Nutrient broth Nutrient broth (Merck or equivalent brand); pH value 7.0 (8 g/l, H2O, 37 °C) (after autoclaving), solubility 8 g/l, bulk density 470 kg/m3, pack size 500 g in plastic bottle. The composition (g/L) of the media should be Peptone from meat 5.0; meat extract 3.0. ( Supplier should be sole agent of the manufacturing company)

25 x 500g

34. Peptone Merck / sigma Aldrich (Supplier should be sole agent of the manufacturing company)

1 Kg

35. Potato dextrose agar Merck or equivalent (Supplier should be sole agent of the manufacturing company) 1 Kg

36. Potato starch Merck or equivalent (Supplier should be sole agent of the manufacturing company) 2 Kg

37. Pikoviskaya agar Merck or equivalent (Supplier should be sole agent of the manufacturing company)

38. Pikoviskaya broth Merck or equivalent (Supplier should be sole agent of the manufacturing company)

39. Pseudomonas agar base Oxoid or equivalent (Supplier should be sole agent of the manufacturing company) 500 g/unit

40. Rappaport-vassiliadis Soya Peptone broth (RVS broth) Dehydrated powder, Microbiology grade 2 x 500g

41.

Reinforced clostridial media Reinforced clostridial media (Merck or equivalent brand); pack size 500g in plastic bottle, pH value after autoclaving 6.6 - 7.0. The composition (g/L) of the media should be Meat extract 10.0; Peptones 10.0; Yeast extract 3.0; D(+)Glucose 5.0; Starch 1.0; Sodium chloride 5.0; Sodium acetate 3.0; L-Cysteinium chloride 0.5; Agar-agar 0.

20x500g

42. Rice Starch Microbiological Grade, Merck/Sigma Aldrich or equivalent 1Kg

43. Sabouraud dextrose agar

Sabouraud 2% dextrose broth (Merck or equivalent brand); pH Value 5.5 - 5.7 (30 g/l, H2O, 22 °C) (after autoclaving), Solubility 30 g/l, bulk density, pack size 500 g in plastic bottle. The composition (g/L) of media should be Peptone from meat 5.0; peptone from casein 5.0; D(+)glucose 20.0.

25x500g

44. Salmonella Shigella Agar Oxoid or equivalent 500 g/unit

45. Selenite broth Oxoid or equivalent 500 g/unit

46. Skim milk powder

Skim milk powder (Merck or equivalent brand) for microbiology; pH value 6 - 8 (20 g/l, H2O, 20 °C), solubility 700 g/l, pack size 500 g in plastic bottle, bulk density 500 kg/m

3.

10x500g

Page 20: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

47. Starch Soluble Microbiological Grade, Merck/Sigma Aldrich or equivalent 2Kg

48. Sucrose Sucrose (Merck or equivalent brand) for microbiology; pH value 7 (100 g/l, H2O 20 °C), pack size 2 Kg in plastic bottle, Hill formula C12H22O11, molar mass 342.29 g/mol, melting point 169 - 170 °C, bulk density 800 - 950 Kg/m3.

15x2Kg

49. Triple sugar Iron agar Dehydrated agar, microbiology grade, differentiate Enterobacteriaceae by three sugar fermentations and hydrogen sulphide production, Oxoid or equivalent

2 x 500g

50.

Tryptone

Tryptone, pancreatic digest of casein (Qxide, Merck / sigma Aldrich or equivalent brand); Should consist of total Nitrogen 13.3% w/w, Amino Nitrogen 3.7 % w/w, Sodium chloride 0.4% w/w, pH (2% solution) 7.3 ± 0.2, pack size 500 gm in plastic bottle. The moisture content should be <5, % ash 7.6, amino % 7.6, pack size 500 g.

15x500 g

51.

Tryptone soy broth Tryptic soy broth (Merck or equivalent brand); Casein-peptone soymeal-peptone broth for microbiology, solubility 30 g/l, pack size 500 g in plastic bottle, bulk density 570 kg/m3, pack size 500 g in plastic bottle. The composition (g/L) of the media should be Peptone from casein 17.0; peptone from soymeal 3.0; D(+)glucose 2.5; sodium chloride 5.0; di-potassium hydrogen phosphate 2.5.

25x500 g

52. Xylose-Lysine-Desoxycholate Agar (XLD Agar) Dehydrated agar, Microbiology grade 2 x 500g

53. Yeast Extract

For use in microbial growth medium, Bio Basic Cat# G0961, Oxide or equivalent. (Supplier should be sole agent of the manufacturing company)

500 g

54 RPMI 1640 Modified media With L-Glutamine, without sodium bicarbonate. MP Biomedicals Inc. Or Sigma

company, packing size 100g/bottle. 2 x 100g

55 Cell Culture Flask 25cm2 SPL Life Sciences or Equivalent 1000 sks

56 Phytohemagglutinin(PHA) Invitrogen. Gibco company pack size 10ml 20 10ml

57 KaryoMAX Colcemid solution Invitogen or Gibco company, cat: 15212-012, pack size 10ml 5 x 10ml

58 Mitomycin C, MP Biomedical Inc. or Invitrogen/ Gibco company, size 2mg/Vial 3 x 2mg

Page 21: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

LIST OF KITS (D)

S. No. Description Item Specification Quantity Required

1. AbsoluteIDQ p150 Kit (MS-based Metabolomics Kit) For 1000 reactions or samples 1 kit

2. Albumin bromocresol green (BCG Kit) for blood/plasma analysis on clinical chemistry analyzer (Microlab300) 8 x (5x25 ml)

3. Alkaline Phosphatase Kit for blood/plasma analysis on clinical chemistry analyzer (Microlab300) 5 x (5x25 ml)

4. ALT/ALAT Kit for blood/plasma analysis on clinical chemistry analyzer (Microlab300) 5 x (5x25 ml)

5. Aluminium Kit Merck (Cat #1.14825.0001) 3 kits

6. Antimicrobial discs (20 different antibiotics) Antimicrobial Susceptibility Testing as per CLSI Guidelines 2014-15 40 x (50 discs /vial)

7. Arsenic Kit Merck (Cat # 1.17927.0001) 3 kits

8. AST/ASAT Kit for blood/plasma analysis on clinical chemistry analyzer (Microlab300) 5 x (5x25 ml)

9. Barium Kit Cat#1206499 (Merck) 02

10. Bile acids Kit (Standardized bile acids phenotyping in mouse and man)

For 1000 reactions or samples 1 kit

11. Boron Kit Merck (Cat # 1.14839.0001) 3 kits

12. Brucella C ELISA kit (Antibody test) SVANOVA (10-2701-02) or equivalent 1 kit

13. Cadmium Kit Merck (Cat # 1.01745.0001) 3 kits

14. Calcium Kit for blood/plasma analysis on clinical chemistry analyzer (Microlab300) 5 x (2x50ml)

15. Cholesterol Kit for blood/plasma analysis on clinical chemistry analyzer (Microlab300) 5 x (5x25 ml)

16. Chromium Kit Cat# 1.14758.0001 (Merck) 03

17. Cobalt Kit (1-10mg/l of Co) Cat #110002.0001 (Merck) 02

18. Copper kit Cat#1.14767.0001 (Merck) 02

19. Creatinine Kit for blood/plasma analysis on clinical chemistry analyzer (Microlab300) 5 x (2 x 100 ml)

20. Cyanide Kit Merck (Cat # 1.14800.0001) 3 kits

21. DNA extraction kit Thermo Scientific, Invitrogen or equivalent. (Origin should be Europe or USA;

Supplier should be sole agent of the manufacturing company) 500 preps

22. DNA Isolation Plant Mini Kit MP BioMedicals, USA or equivalent. (Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company)

05

23. DNA size standard kit 400 bP and 600 bP 02 each

24. ELISA kit for FMDV antigen detection ELISA OF FMDV O, A, ASIA 1 and C 5 plates/kit (6 tests/plate) (IZS-Brescia & IAH-

Pirbright or equivalent) 1 kit

25. EZ-10 Spin Column DNA gel extraction Kit Biobasic, fermentas or equivalent (Origin should be Europe or USA; Supplier should

be sole agent of the manufacturing company) 1000 preps

26. EZ-10 Spin Column PCR products purification kit Biobasic, fermentas or equivalent (Origin should be Europe or USA; Supplier should

be sole agent of the manufacturing company) 1000 preps

27. EZ-10 Spin Column plant genomic DNA Min-preps kit Biobasic, fermentas or equivalent (Origin should be Europe or USA; Supplier should

be sole agent of the manufacturing company) 1000 preps

28. EZ-10 Spin Column plasmid DNA Min-preps kit Biobasic, fermentas or equivalent (Origin should be Europe or USA; Supplier should

be sole agent of the manufacturing company) 1000 preps

Page 22: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

29. FastDNA Spin Kit for Soil

MP BioMedical, (USA), Rapid and reproducible sample lysis with the FastPrep®-24 or FastPrep® FP120 Instrument.

05

30. FastRNA Pro Green Kit is used to isolate total RNA from any type of plant and animal tissue or cultured cells.

(MP BioMedical USA), Rapid and reproducible sample lysis with the FastPrep®-24 or FastPrep® FP120 Instrument.

05

31. Fluoride Kit Merck (Cat # 1.14598.0001) 2 kits

32. Fragment analysis test sample

33. Free fatty acid quantitation kit

34. Fungal DNA isolation kit, Cat #26200, Cat #26900, Norgen-Biotech-Corp.

35. GelRed™ Nucleic Acid Gel Stain, 10,000X 5 ml

36. Gene Jet Plasmid Mini Prep Kit K0503/Fermentas

37. GF-1 Blood DNA Extraction Kit 1 box (for 100 samples) 5 x (1x100 samples)

38. Glucose (GO) Assay Kit (for 20 assays) 20 assays per kit 20 kits

39. Gram staining kit for microscopy

Gram staining kit for microscopy (sigma or equivalent brand); components of 1 Kit contains 250ml each of following: 94448 Gram′s crystal violet Solution, 90107 Gram′s iodine Solution, 75482 Gram′s Decolorizer Solution, 94635 Gram′s safranin Solution. Flash point 20 °C, grade for microscopy.

10x1 kit

40. Hemoglobin kit for blood/plasma analysis on clinical chemistry analyzer (Microlab300) 5 x (2x25ml)

41. Human C-Reactive Protein ELISA kit for blood/plasma analysis on clinical chemistry analyzer 10 kits

42. Human Glycated Hemoglobin A1c (HbA1c) Kit for blood/plasma analysis on clinical chemistry analyzer 10 kits

43. Human Insulin ELISA kit for blood/plasma analysis on clinical chemistry analyzer 10 kits

44. ID Screen® PPR Antigen Capture ELISA kit Sandwich ELISA, Anti-PPRV Nucleoprotein antibody, Anti-PPRV-NP Mab-HRP

conjugate (concentrated 10X), Used for variety of sample types, including oral, nasal or rectal swabs as well as tears, tissue samples, gum debris or PBMC

1

45. InsTAclone PCR Cloning Kit Thermo-Fermentas or Equivalent (Origin should be Europe or USA; Supplier should

be sole agent of the manufacturing company)

46. Iron Kit Merck (Cat # 1.14761.0002) 3 kits

47. Lead Kit Merck (Cat # 1.09717.0001 ) 3 kits

48. Manganese Kit Merck (Cat # 1.14770.0001) 3 kits

49.

Megascript T7 (In vitro transcription kit) 1/Enzyme Mix (SP6, T7, or T3) 10X Reaction Buffer† (SP6, T7, or T3) ATP Solution(SP6, T7, or T3) CTP Solution (SP6, T7, or T3) GTP Solution (SP6, T7, or T3) UTP Solution (SP6, T7, or T3) Nuclease-free Water TURBO DNase (2 U/µL) Ammonium Acetate Stop Solution 5 M ammonium acetate, 100 mM EDTA Lithium Chloride Precipitation Solution7.5 M lithium chloride, 50 mM EDTA

1

Page 23: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

50. Minimum Inhibitory Concentration Test Strips (20 different antibiotics)

Antimicrobial strips with Concentration gradients. Comparable results with CLSI, BSAC or EUCAST methods

20 x (10 strips/cartridge)

51. Nickel Kit Merck (Cat # 1.14785.0001) 3 kits

52. Nitrate Kit Merck (Cat # 1.14773.0001) 3 kits

53. Nitrite Kit Merck (Cat # 1.14776.0002) 3 kits

54. RNA extraction kit Rapid purification of high quality viral RNA in 30 minute. Eliminates the use of phenol/chloroform, Thermo-Fermentas, Invitrogen or equivalent (Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company)

100 preps

55. PCR clean up Kit Thermo-Fermentas, Invitrogen or equivalent (Origin should be Europe or USA;

Supplier should be sole agent of the manufacturing company) 1000 Rxn

56. pENTR™/D-TOPO® Cloning Kit 20 reactions/pENTR⁄D-TOPO® vector, dNTPs, salt solution, sterile water Thermo-

Fermentas, Invitrogen or equivalent (Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company)

100 Rxn

57. Phenol Test Kit Range (0.002-5.00 mg/l) Merck (Cat # 1.00856) 3 kits

58. Phosphate Test Kit) Merck (Cat # 1.14598.0001) 2 kits

59. Plasmid purification kit Thermo-Fermentas, Invitrogen or equivalent (Origin should be Europe or USA;

Supplier should be sole agent of the manufacturing company) 5x250 preps

60. Quick gel extraction kit Invitrogen, Thermo-Fermentas or equivalent (Origin should be Europe or USA;

Supplier should be sole agent of the manufacturing company)

61. QIAamp Viral RNA Mini Kit (250) For 250 RNA preps/250 QIAamp Mini Spin Columns, carrier RNA, Collection Tubes (2

ml), RNase-free buffers 1

62. Qiamp DNA mini kit Qiagen (51306) or equivalent 1 kit

63. Real time PCR quantitative KIT PCR Kit for HCV Quantitative detection of HCV in Blood Plasma. 01X100Rxn

64. RNA/DNA/Protein purification kit, Norgen-Biotech-Corp.( Cat # 24000)

65. RNeasy Mini Kit Biobasic or equivalent 50 preps

66. RNEASY Plant Mini Kit (MP BioMedicals, USA), Rapid and reproducible sample lysis with the FastPrep®-24 or FastPrep® FP120 Instrument.

05

67. RevertAid H Minus First Strand cDNA Synthesis Kit Fermentas-Thermo, Cat No. K1632 or equivalent

68. RevertAid™ First Strand cDNA Synthesis Kit Fermentas-Thermo, Cat No. K1622 or equivalent

69. Silicate Kit Merck (Cat# 1.14749.0001) 3 kits

70. Silver Kit Merck (Cat # 1.14831.0001) 3 kits

71. Soil DNA Isolation Kit MP Biomedical USA or equivalent 4 kits

72. Soil RNA Isolation Kit MP Biomedical USA or equivalent 3 kits

73. Spurr Low Viscosity Embedding Kit (For TEM) Sigma, CAT # EM 0300 01 Kit

74.

SuperScript® III First-Strand Synthesis System The SuperScript® III First-Strand Synthesis System for RT-PCR with; • Oligo(dT)20 (50 µM), 50 µl • Random hexamers (50 ng/µl), 250 µl • 10X RT buffer, 1 mL • 0.1 M DTT, 250 µl • 25 mM magnesium chloride, 500 µl • 10 mM dNTP mix, 250 µl

2

Page 24: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

• SuperScript III RT (200 U/µl), 50 µl • RNaseOUT (40 U/µl), 100 µl • E. coli RNase H (2 U/µl), 50 µl • DEPC-treated water, 1.2 mL • Total HeLa RNA (10 ng/µl), 20 µl

75. TA Cloning Kit Thermo-Fermentas, Invitrogen or equivalent (Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company)

10 x 30 runs

76. Total Nitrogen Test Kit Range (0.5-15.0 mg/l) Merck (Cat # 1.00613) 2 kits

77. Total RNA purification kit,

Norgen-Biotech-Corp. (Cat # 37500)

78. Transmission Electron Microscopy Kit Sigma or Merck 1 kit

79. Triglyceride kit for blood/plasma analysis on clinical chemistry analyzer (Microlab300) 5 x (4x100 ml)

80. Urine analysis dipstick strips for the analysis of Ketones, Glucose, Protein, pH etc. 20 x (100 in a box)

81. Vitamin D Kit (Mass Spectrometry Based Accurate Assessment of Vitamin D Status)

For 1000 reactions or samples 1 kit

82. Vitamin D Reagents (Mass Spectrometry Based Accurate Assessment of Vitamin D Status)

For 1000 reactions or samples 1 kit

83. Zero Blunt® PCR Cloning Kit K2750-40 / invitrogen

84. Zinc Kit Merck (Cat # 1.14832.0001) 3 kits

LIST OF GLASSWARE (E)

S.No. Description Item Specification Estimated Qty/unit

1. Additive gel & clot activator blood test tube 5ml , 100/ box 5 box

2. Anaerobic flasks (100ml, 250ml)

3. Büchner flask with socket and PTFE connector (Pyrex or equivalent), Socket Size 19/26, Capacity 100 mL, Height 107 mm 02 Nos

4. Büchner flask with socket and PTFE connector Pyrex or equivalent, Socket Size 24/29, Capacity 250 mL, Height 145 mm 02 Nos

5. Buchner funnel (stem with cone) Approx. Funnel Dia 71mm, Disc dia. 55mm, Sint Disc Porosity 3, Cone Size 19/26 02 Nos

6. Buchner funnel (stem with cone) Approx. Funnel Dia 71mm, Disc dia. 55mm, Sint Disc Porosity 3, Cone Size 24/29 02 Nos

7. Chromatography columns Socket Size 29/32, Capacity 500 mL, Dia 30 mm 02 Nos

8. Cold trap body Pyrex or equivalent, Socket Size 24/29 Long height 02 Nos

9. Cold trap head Pyrex or equivalent, Cone Size 34/35 02 Nos

10. Cold trap head Pyrex or equivalent, Cone Size 24/29 Short Height 02 Nos

11. Cold trap head Pyrex or Equivalent, Cone Size 19/26 02 Nos

12. Condenser (Coil with ground cone and socket) Pyrex or Equivalent, Cone size 19/26, Socket size 19/26, Approx. effective length 165 mm, Approx. overall length 305 mm, Approx. surface area 2 x 102m

2

02 Nos

13. Condenser (Coil with ground cone and socket) Pyrex or Equivalent, Cone size 24/29, Socket size 24/29, Approx. effective length 165 mm, Approx. overall length 310 mm, Approx. surface area 2 x 102m

2

02 Nos

14. Digestion tubes neck size 40/38 mm Pyrex or Equivalent 24 Nos

Page 25: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

15.

Disposable inoculating loop with needle It must feature a certifiably calibrated 1µL loop on one end and a needle point on the

other end. It must have 20cm length. Gamma irradiated and sterilized packing. Clear,

plastic.

50

16. Erlenmeyer flasks (1000, 500, 250 & 100ml) It should have thick-walled body and long, tapered outside counter. Its top should be

reinforced and tooled for maximum mechanical strength. It must have durable white ceramic enamel scales. It should be fully autoclavable to 121ºC.

10 each

17. Flask pear shape (100, 50 & 25 ml) Pyrex or Equivalent, 02

18. Glass Beaker (pyrex or equivalent, 1000, 500, 250 & 100 ml) It should be made up of borosilicate glass includes thick, uniform walls. It must feature a

beaded top with a spout. It must have durable matte finish marking area and white graduated scale. It must be of low form and autoclavable to 121C.

20 each

19. Glass Bottles, REAGENT NARROW NECK, GLASS STOPPER Capacity 500 mL Dia. 89 mm Height with stopper 175 mm Stopper Size 24/29

05

20. Glass Bottles, REAGENT NARROW NECK, GLASS STOPPER Capacity 1000 mL Dia. 111 mm Height with stopper 214 mm Stopper Size 29/32

05

21.

Glass covers slips It should be made up of pure white borosilicate glass with excellent chemical resistance and flatness. It should be free from bubbles and scratches. It should not emit disturbing self-fluorescence under UV light. It should be square in shape and manufactured in accordance with DIN ISO 8255-1.

10 box

22.

Glass cylinders (Measuring, 1000, 500, 100 & 50 ml) It should be made of borosilicate glass, with a reinforced top bead, pour spout and a hexagonal base. It should feature a durable white ceramic enamel metric scale externally.. Must be certifiably calibrated to deliver. Cylinder of 1000 ml capacity must have removable plastic LDPE neck collar.

5 each

23. Glass Funnels

24. Glass Petri Plates & lid

Reinforced beaded edges to resist mechanical breakage and to aid in centering the bottom inside of the cover, Optically clear with no color tinge and should remain clear after repeated sterilization cycles at 122ºC. The covers should be inscribed in white with the word "cover" and bottom should be inscribed in blue with the word "bottom". The lid should feature a fused-on white ceramic enamel marking spot and bottom piece should be marked with a vertical arrow on the side. It must have dimension of 100×15 mm. It should be KIMAX brand or equivalent,

200 plates

25. Glass Plates for SDS 20/20 cm 5 plates

26. Glass rods

27.

Glass slides It should be made up of plain white glass with low iron content. It should not have green tint and feature ground and polished edges for safe handling. It must be corrosion resistant. It should be 25×75mm in size and 1mm thickness. (Origin should be Europe or USA)

10 box

28. Glass Spreader 20 No.

29. Glass test tubes (18 x150 mm & 13x100mm length) It should be made up of borosilicate/pyrex glass with round bottom. Its rims should be fire polished. It should be well annealed, resistant to heat and chemical. It must be autoclavable to 121ºC. (Origin should be Europe or USA)

500 tubes each

30. Glass tubes (screw capped, 10 ml & 30 ml) It should be made up of borosilicate/pyrex glass with round bottom. It must have durable matte finish white marking area. It should be screw capped. Cap should be made of

100 tubes each

Page 26: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

phenolic compound and must have glued-in white rubber liner. Screw cap should be resistant to temperature and steam. It must be autoclavable to 121ºC.

31. Glass vials PTFE Lined Cap Clear 20 ml, 100/pk, Clear 33 expansion borosilicate clear glass (Type 1, Class A) 01 pk

32. HPLC glass vials (2 ml, 4 ml & 11.6*32mm capacity) Origin should be Europe or USA 500 each

33. K3/2Na EDTA vacuum blood container 5ml , 100/1 box 5 box

34.

L shaped disposables loops It must be made up of medical grade polypropylene. The bend must have at least 100º

±1 interior angle. It should be gamma sterilized and sterile packed. Handle Length should

be atleast 140 mm and spreading rod of 35mm length.

50

35. LCMS Certified Clear Glass vial, Screw Neck Vial, with Cap and Preslit PTFE/Silicone Septa,

100/pkg, 12 x 32mm, 2 mL Volume 5 box

36. Pasteur pipettes (Glass, 220 mm, 150 mm) 02 each

37. Reagent (media) bottles (50,100,250,500,1000 & 2000ml)

Borosilicate glass with wide opening (30mm I.D at least), repeatedly autoclavable up to 180ºC, permanent external white enamel graduation in mL and a marking spot, cap should be screw threaded, made of linerless polypropylene, GL45 in size and autoclavable to 140ºC. It must have a replaceable, dip-free, clear polypropylene pour ring incorporated into the bottle design. SIMAX brand or equivalent

20 Nos each

38. Reusable Glass syringe with needle

1 mL, Metal Luer lock tips, borosilicate glass barrel and plunger 05

39. Reusable Glass syringe with needle

5 mL, Metal Luer lock tips, borosilicate glass barrel and plunger 03

40. Reusable Glass syringe with needle

10 mL, Metal Luer lock tips, borosilicate glass barrel and plunger 02

41. Round bottom flask (capacity 250 & 100ml) Short neck (Pyrex OR Equivalent) Socket Size 19/26 05 each

42. Rubber plugs (stopper) of above flasks

43. Screw cap micro tubes with conical base (2mL) 5000

44. Separating Funnels 500 ml /Pyrex or Equivalent 10 Nos

Page 27: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

LIST OF PLASTICWARE/LABWARE/MISCELLENIOUS (F)

S. No Description Item Specification Estimated quantity/

1. 96-Well Fluorescence Microplates (Black) Polystyrene, 96 well plates, sterile, flat bottom. Perkin Elmer (Cat No. 16005270) or VWR (Cat No. 62403-156) or FisherScientific

(Cat No. 07-200-590), Corning (Cat No. 3915) or equivalent (Origin should be Europe or USA) 100 No.

2.

96 well microtiter plate with lid Sterile, clear, and rigid made up of crystal grade polystyrene, rectangular geometry, having ridges on the plate top for stable stacking. The wells should have raised rims. Both the plate and lid should have beveled corners. It should feature an alpha neumeric notation printed on plate top and the wells must be flat bottom. Plates should have working volume of up to 250µl in each well. The plates must be in Individual plastic packing (Gamma sterilized, non- pyrogenic, untreated well surface) with brand, cat and lot no printed on it. The plates must be certifiably compatible with Spectramax 384 machine. (Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company)

250 nos.

3. 96-Well Fluorescence Microplates (White)

Polystyrene, 96 well plates, sterile, flat bottom. Perkin Elmer (Cat No. 6005320) or VWR (Cat No. 62403-150) or FisherScientific (Cat No. 07-200-589), Corning (Cat No. 3912) or equivalent (Origin should be Europe or USA)

100 No.

4. Aluminium foil 73.5 SA.FT. (8.33 YDS x 18 IN) 3.48m2(7.62m x 45.7cm) Diamond USA 10

5.

Blue Tips (1ml) Universal Pipette Tips, 100 – 1000ul, DNase and RNase Free, Non Pyrogenic, Fit wide variety of micro- pipettors

and multi-channels micropipetors. Universal grip design, provided with calibration lines at specific volumes,acting as visual checks to minimize any risk of errors during sample aspiration.Made of high quality, low adhesion, polypropylene. Biologix or equivalent. (Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company)

10 packs

(1000/pack)

6. Capillary Array DNA Separation, for Beckman coulter CEQ 8000

33cmx75um, 02

7. Cell 50 mm for NOVA 60 Spectroquant Merck 2 Pairs

8. Centrifuge Machine Maximum range (14000 rpm)

9. Centrifuge tube 1.5 ml, Flat and rough cap

10. Centrifuge tube (15 & 50ml)

It must be made up of polypropylene. It must have conical bottom and screw flat cap. Its cap should be closely

tight and have a plug seal . It must have printed white graduations, large white writing patch on the side. It should be certifiably autoclavable to 121ºC and freezable to -90ºC. It should be free form DNase, RNase and endotoxin. It should have gamma irradiated sterilized packing. (Origin should be Europe or USA)

500 pcs

11. Cling film PVC, stretch film, soft, 500 mm width, 10-11 micron thickness, 1000 meters, Lahore plastic or

equivalent 3 pieces

12. Cryo boxes Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company 50

13. Cryo vials (1.8 ml capacity blue cap) Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company 1000pcs

14. Cuvette plastic Disposable semi-micro cuvette, 10 mm path-length, 280 nm - 900nm, 1.5 and 3.0 ml 100/pk 5 x100/pk

15. Cuvette (1ml & 3ml) Quartz

16. Cuvettes for Electroporation

(gap width 1mm)

Polycarbonate, Imbedded aluminum electrodes, sterile, width (Metric) gap 1mm, 90 µL capacity, Fisher(Cat# FB101), Z706078 or BioRad (Cat# 1652089) or equivalent.

50/pack

17. Cuvettes for Electroporation

(gap width 2mm)

Polycarbonate, Imbedded aluminum electrodes, sterile, width (Metric) gap 2mm, ~ 400 µL capacity, Fisher(Cat# FB102) or Sigma (Cat#Z706086-50EA) or BioRad (Cat# 1652086) or GenseeScienitfic (Cat No. 40-101) OR equivalent. (Origin should be from Europe or USA

50/pack

18. Colored Printer

19. Color cartridge

Page 28: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

20. Dialysis Tubing (Exclusion limit-14000Da) Roth-Germany/equivalent . Origin should be Europe or USA; Supplier should be sole agent of the

manufacturing company 30 feet/ roll

21. Dissecting Scissors Stainless steel, straight, 4 ½ inch,Cat# 621760,Carolina 3 No.

22. Dry Bath, Heating Thermo state BIO SAN Bio TDB-100

23. Electric Sockets Multi 20A, 250V

24. Electric Tube rod starter Philips

25. Electric tube rods Philips

26. ELISA plates Polystyrene, 96 well plates, sterile, flat bottom with lid. Thermo Scientific (Cat No. 15031) or VWR (Cat

No. 25381-054) or Sigma (Cat No. CLS 3590) or equivalent 100 No.

27. ELISA reservoirs Thermo or equivalent

28. Eppendorf stand Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company 10

29. Electrical Field balance (Rechargable) 01

30. Extended filtered tips (10ul) Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company 1000pcs

31. Falcon tube stands (15 & 50 ml) 12 each

32. Falcon tubes (Flat top Cap, 15 ml, 50 ml) Cell centrifugation applications, Polypropylene, sterile, Biologix or equivalent Origin should be Europe

or USA; Supplier should be sole agent of the manufacturing company 500/pack

33. Filter paper sheets For solution filtration, 26’/16’, 80 grams. 100 pcs.

34. Filter Papers diameter 47 mm Millipore, Filter lab or equivalent 5 Pks

35. Forceps

Lab Set Forceps Conventional forceps, stainless steel. Cat# 72957-06Electron Microscopy Science. 1x(6/set)

36. Glass spreader For spreading of microbiological culture on agar plates, 90° Bend, Cat# 89166-788, VWR or equivalent 10 No.

37. Gel trays Small & Medium

38. Gel combs Small & Medium

39. Inoculating/streaking loop & Loop wire 25-gauge nichrome wire with a 3mm loop attached to an 8` aluminum handle. Cat# 43050002-1, Bio

world or equivalent. 10 Nos. & 10 meter

40. Gloves (Blue nitrile), powder free Extra large (Omnilab 5421393 or equivalent), Large (Omnilab 5421392 or equivalent), Medium (Omnilab 5421391 or equivalent), Small (Omnilab 5421390 or equivalent)

41. Gloves (disposable embossed polythene) Medium & Large (50/pk) 200 Pk each

42. Gloves (Latex, disposable) Non powdered, Large and Medium, Top Malaysia or equivalent

43. Gloves (Nitrile examination, latex free) Medium & Large (600/box) 50 box each

44. Gloves (Polythene) Home care, medium

45. Gloves (surgical) Large & Medium size latex or equivalent

46. GMO Rice standards

47. Hats 04

48. Hemocytometer (cell counter) Life Technology or equivalent 01 No.

49. HP Laser jet printer Pro M201DN

50. Juicer Grinder Cups 300

51. Lab Spoon/Spatula, 304 SS different sizes 03 Nos.

52. LCMS Certified Clear Glass vial, Screw Neck 100/pkg, 12 x 32mm, 2 mL Volume 5 box

Page 29: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

Vial, with Cap and Preslit PTFE/Silicone Septa,

53. Liquid nitrogen container Capacity 60 liter, Neck diameter 8.5 inch/21.5 cm 01 Nos.

54. Membrane screw caps (2 mL) 5000

55.

Microcentri-fuge tubes (1.5 ml) It should be made up of polypropylene with side labeling surface. It must have flat, pierceable and frosted caps having smooth surface and ergonomic design. Tubes should be fit in standard rotors and should be spin up to 14,000g. It should be free form DNase, RNase and endotoxin. It should be autoclavable to 121ºC and freezable to -90ºC . Origin should be Europe or USA; Supplier should be

sole agent of the manufacturing company

1000 No.

56. Microcentrifuge tubes racks It should be made up of polyproylene and must hold atleast eighty 1.5ml to 2ml tubes. The slots should

be numerically coded on rack top. It should be repeatedly autoclavable to 121ºC and freezable up to -90ºC. It should have a feature of neoprene feet to keep it stable on the workbench.

10

57. Microcentrifuge tube with lid (Eppendorf) (2mL) Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company 4000

58. PARAFILM® M

Material should be thermoplastic, semi-transparent, odorless , colorless, flexible, moldable, waterproof, self-sealing and rolled into a 4IN. ×125FT. interwound with paper. It should be provided with a foldable dispensing cardboard box with brand/cat no printed on it. The manufacturing origin must be USA/Europe and of M brand or equivalent.

20 Rolls

59. PCR plate 96 well

60. PCR tubes (0.2mL & 0.6 ml)

It should be made of polycarbonate and must have a feature of contamination shield on a hinged lid. It should be compatible for in all major brands of thermo cyclers with 0.2mL or 0.6 mL block format. It must have side labeling surface and thin walls for temperature precision. It must have flat, pierceable and frosted caps with smooth surfaces and ergonomic design. It should be certifiably free form DNase, RNase and endotoxin. It should be autoclavable to 121ºC for 20 minutes and freezable to -90ºC . Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company

1000/Pk

61. PCR tubes storage box It should be made up of polypropylene and must hold ninety six 0.2mL tubes. It should be autoclavable

to 121ºC and freezable up to -90ºC. 10

62. Pestle & Mortars Rough Surface 12

63.

Disposable petri plates with lid (Fully stackable)

It should be molded from biomedical grade polystyrene. It must have clear and flat surface. It must include a raised straight ridge around top for stable stacking and three venting ribs in the underside of the lid to prevent condensation buildup. It should be 100×15 mm in diameter and should be packed in sealed sleeves of 20 (Gamma sterilized). Biologix-USA/NEST/ equivalent. Origin should be Europe or

USA; Supplier should be sole agent of the manufacturing company

20 plates/pk

64. Pipette sets, (2-20 ul, 10-100 ul, 200-1000 ul ) Gilson, Cat Nos. F144563, F144564, & F144566

65.

Pipette tips [1000ul (blue), 200ul (white & yellow), 100ul, 10ul]

Made of polypropylene with a ribbed top and beveled end. certifiably be endotoxin, RNase and DNase free. Tips should have a low binding feature and must be free of protease and human DNA. Its outer wall should be graduated with flush calibration lines. It should be certifiably compatible with VITLAB micropipette. It must be autoclavable to 122ºC. Biologix, USA or equivalent. Origin should be Europe

or USA; Supplier should be sole agent of the manufacturing company

20 Packs each (1000/pack)

66. Plastic PVC container / Plastic pots 15x12x9, 18x12x11, 12x10x6 inches (LxWxD) 100 each

67. Polyethlyne sheet thickness 6′×4′×4″ (foil) with

baorde (2″ Width & 1″ Height) Diamond Jumbolon, Jilani plastic Lahore or equivalent 500 Sheets

68. Wooden Gola 2” x 3” and 1”x1”

69. Lamination Sheet 8’ x 4’ x 3/4” 06 Nos.

Page 30: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

70. Nail (Powers) 3 Kg

71. Plastic Gotti 100pc

72. Steel Nail 1” 1 pkt

73. Aluminum Racks (50ml tube) It should be made up of anodized aluminum. It must be resistant to corrosion and chemical attack. It

should have atleast 6 slots for 50ml tubes. 10

74. Aluminum Racks (15ml tube) It should be made up of anodized aluminum. It must be resistant to corrosion and chemical attack. It

should have atleast 6 slots for 15ml tubes. 10

75. Centrifuge tubes racks (50ml) Racks must hold 50ml plastic centrifuge tubes. It must made up of epoxy coated steel wire and have

atleast 8 slots for tubes. It should be autoclavable to 121ºC. 10

76. Centrifuge tubes racks (15ml) Racks must hold 15ml plastic centrifuge tubes. It must contain epoxy coated steel wire and have 8 slots

for tubes. It should be repeatedly autoclavable to 121ºC. 10

77. Rectangular cell 10 mm for Spectroquant Merck (Cat # 1.14946.0002) or equivalent 1 Pair

78. Reusable glass syringe with needle (1ml, 5ml & 10ml)

Metal Luer lock tips, borosilicate glass barrel and plunger 05/03/02

Nos.

79. Screw cap vials (amber and transparent both) 10 ml 100 vials

each

80. Shoes (Long, Field) 2 pairs

81. Silica thin layer chromatography plates Merck TLC plates or equivalent (pack of 25 plates) 10 packs

82. Slip-tip disposable syringe (5 ml & 3 ml) 5 mL 1000 Nos.

each

83. Spray bottle (500 ml) 02 Nos.

84. Spray Tank (Plastic) 2.5 L & 20 L

85. Stand with rod Material - Stainless Steel 02

86. Steel clamp bossheads Omnilab 5224284 or equivalent 05 Nos.

87. Steel support clamps Clamping range 25mm (Omnilab 5224470 or equivalent) & 40mm (Omnilab 5224471 or equivalent) 05 Nos.

88. Sterile culture swabs For clinical specimen collection and transportation system, each swab separately packed, Lot number and expiration date printed on tube labels

100 swabs/Pk

89. Stir bar retriever, flexible 14-3/4"L 02 Nos.

90. Sucker bulb

91. Surgical blades Scalpel Blades - #10, curved, 30 mm, 0.4 mm thickness, carbon steel, fine scientific tools or equivalent. 50 No.

92. Syringe (1 mL, 3 ml, 5 ml, & 10 ml) Sterile, disposable syringe with needle 26G x 5/8’, 24G x 1’, 22G x 1 ¼’, SHIFA SYRINGE 1CC or

equivalent 500 Nos.

93.

Disposable Syringe, (5 and 50ml) It must incorporate a BD leur lock tip and should be individually wrapped. It must have bevel-oriented needles. Needles should be made up of stainless steel with translucent color-coded polypropylene hubs. Thin wall needles should have a bigger I.D. without any increase in the O.D. It should be sterilized with Gamma or Ethylene oxide (EO) gas. (BD brand or equivalent)

4000 & 1500

94. Syringe filters 0.2 µm

It must have low protein binding durapore (PVDF) membrane and should be non-pyrogenic. It must not contain natural rubber latex components. Each filter must have a female Luer Lock inlet and a male slip Luer outlet. It's pore size should be of 0.2µm and diameter of 33mm. It should be gamma sterilized. It should be of Merck-Millex brand or equivalent. It must have individual blister packing. Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company

4 x 50

Page 31: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

95. Syringe filters,0.45 µm A 33 mm diameter sterile syringe filter with a 0.45 µm pore size hydrophilic PVDF membrane. Cat#

SLHV033RS, Merck Millipore or equivalent. 50 No.

96. Syringe filters, 0.22 µm A 33 mm diameter sterile syringe filter with a 0.22 µm pore size hydrophilic Polyethersulfone (PES)

membrane, Cat# SLGP033RS, Merck Millipore or equivalent. Supplier should be sole agent of the

manufacturing company

50 No.

97. Telephone set PTCL (Land Line)

98. Test tube stands

99. Thermal paper for imaging of DNA agarose gels Fine, easy to use in every gel doc.Size:110mm wide X 20m long, approximately 270 prints per roll. To be used use with the following Sony printers: UP-890MD, UP-895MD, UP-D895, UP-897MD, UPP-110S and UP-D897

Rolls

100. Thermometer 04

101. Thin layer chromatography plates Silica gel 60 F254, Aluminium Sheets 20 x 20 cm. Supplier should be sole agent of the manufacturing

company 25 plates/PK

102. Tip boxes 1 ul, 10ul, 200ul,1000ul,5000ul 1ml, PCR tubes box, 1 ul tip box Origin should be Europe or USA;

Supplier should be sole agent of the manufacturing company

103. Tips 1-20ul yellow tips universal Fit pipette Origin should be Europe or USA; Supplier should be sole agent of the manufacturing company

104. Universal filter pipet tips (10 µl) Sterilized, RNase & DNase Free, Polypropylene, Autoclavable, Graduated. Origin should be Europe or

USA; Supplier should be sole agent of the manufacturing company 10 Racks of

96 Tips

105. Vacutaners EDTA, K3 Lab vac/BD or equivalent 1000 Nos

106. Vacutaners Lithium Heprain Lab vac/B or equivalent 500 Nos

107. Vacutaners Plain Lab vac/BD or equivalent 500 Nos

108. Vacutainers Gel (3ml) 300 nos.

109. Veterinary needle 16G 2 box

110. Vertical freezer

111. Wash bottle SL/SH DIST.WATER 500mL 05 Nos.

112. Wash bottle SL/SH LDPE 500ML WHITE 05 Nos.

113. Wash bottle SL/SH ACETONE 500ML 05 Nos.

114. Wash bottle SL/SH METHANOL 500ML 05 Nos.

115. Whatman cellulose chromatography paper 130 mm/30 min flow rate Width × Length 20 cm × 20 cm basis weight 87 g/m2 (Sigma Aldrich, cat #

WHA3001861) or equivalent 2 packs 100/pack

116. Whatman chromatography 1 20cm × 20cm (100/pk) 5 Pks.

117. Extension wire 5 pk

118. pH strips

119. Plastic Cylinders Volume 250, 500 and 1000 ml

120. Plastic Jug (Scaled) 5 L

121. Plastic Baskets Medium & Large

122. Ice Container

123. Tape stand Medium and Large

124. Rubber seal for distillary 02

125. Heating rod for distillary 06

126. Face mask filter Face mask used for air/dust filter in laboratory 50 x 5 box

Page 32: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

LIST OF GAS REFILLING (G) (Please quote rates /m3)

S. No Description Item Specification Estimated quantity/

1. Nitrogen 99.99%

2. Oxygen 99.99%

3. Helium 99.99%

4. Hydrogen 99.99%

5. Acetylene 99.99%

6. Argon 99.99%

7. Nitrous oxide 99.99%

LIST OF STATIONARY ITEMS & MISCELLANOUS(H)

S. No Description Item Specification Estimated quantity/

1. Ball point pens Signature, Dollar, Jet (Black and Blue) 100

2. Binder clips Small

3. Bleach Liquid 04

4. Brushes for washing Different sizes

5. Color pages Multiple colors

6. Cotton rolls Large 05

7. Detergent ( Gel/powder for washing glass ware and utensils

Powder (Vim, Max dishwash), Liquid (lemon Max, large bottle)

8. Dusters

9. Envelopes White A4 Size (10"x12"), Golden Crafit Paper (A4 Size, 4"x9" Size and 4"x6" Size)

10. Envelopes (brown) A4 size 1000

11. Envelopes, (NIBGE Logo) (24cmX11cm)

12. Eraser Pelikan 20

13. Facemask Normal Size

14. File Covers Soft & hard 50 each

15. File Covers (NIBGE) 2000

16. File Folders (Office)

17. Files A4 Plastic files 36

18. Flapper 1000

19. Fluid 10

20. Foam Sheet 1.5 x 1 meter

21. Folder No.556 15

Page 33: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

22. Glass wool

23. Glue sticks Large (UHU)

24. Handwash Dettol/lifebuoy Skincare Liquid hand wash (anti-bacterial)

25. High lighter Yellow (Schneider)

26. Lab Coats Medium size 10

27. Lead pencils Deer 100

28. Lighter (Flammable) 10

29. Locks (Drawer’s) 20 Nos.

30. Marker (Silver color)

31. Markers (Board, Blue & Black) 12

32. Markers (labelling, bold & Fine tip) Universal Permanent Marker 222F/ 0.7mm (Schneider), Permanent Marker 2 mm (allmark), Dollar etc 12

33. Match Boxes

34. Paper Clips Regular size

35. Paper Ream A-4 200

36. Papers (Glazed) 5 ream

37. Pin remover

38. Pins (Push)

39. Pins (Stapler) Regular size (24/6)

40. Pins (Thumb) Packs 10

41. Plastic Bags Clear 5'' x7''

42. Printer cartage HP LaserJet 2300

43. Registers 200 Sheets

44. Registers (Attendance) Medium 02

45. Rubber Bands Large pack

46. Ruler Steel/12 inch, 1foot 05

47. Sharpener Dux

48. Sheets (Transparent Binding)

49. Soap Dettol Cool (anti-bacterial)

50. Stapler Regular size (26/6-24/6), Dux etc.

51. Stapler (Heavy duty) Binding for more than 100 sheets

52. Sticky notes 3 x 3 “

53. Tag (cotton) 50 pk

54. Tags Sizes (2.6"x3.6" ) & (4cmX5cm) With Ring And Thread,

20 pkt of

100

55. Tape (binding)

56. Tape (Packing) 2 inch

57. Tape (Paper) 1” Nichiban

58. Tape (Scotch) 1 & 2inch, Transparent

59. Tissue Box Large (Rose Petal)

Page 34: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

60. Tissue Paper Jumbo kitchen rolls (Rose Petal) 05

61. Tissue Paper Toilet rolls (Rose Petal), 4 inches width, 4.25 inches length, 500 sheets long

62. Tissues (Lens cleaning) Lens cleaning tissues, Cat# 2105 841 Whatman No.105 or equivalent 10 packs

63. Toner catraige inkjet #22 & inkjet #27

64. Toner recycling Laser Jet (12A)

65. Toner refill Laser Jet

66. Tooth Picks Packs 50

67. Whitener

68. Writing pads 7.6 x 10.2 “ 10

69. Zipper Bags Sizes (5"x7"), (4'' x 6'') & (3.5"x5") (10 cmx 15 cm) & A4

LIST OF IT ITEMS (H)

S. No Description Item Specification Estimated quantity/

8. CD Sony 10 pkt

9. DVD Sony 05 Pkt

10. Hard Disk WD/Segate 10

11. Lan connector Rj45 2 pkt

12. RAM 2 GB 20

13. Server Dell T110 or above 04

14. Telephone sets Fix Line 02

15. UPS 660w 10

16. UPS batteries

17. Battery (Cell) 1.5 Volt (Size AA & AAA) 10

Page 35: LIST OF CHEMICALS (A) list-NIBGE 2017F 31-03-2017 FINAL Dr Kalsoom.pdf · LIST OF CHEMICALS (A) S. No. Description Item Specification Quantity Required 1. A mino acid mix (20 AA)

LIST OF FORM ITEMS (I)

S. No Description Item Specification Estimated quantity/

1. Animal feed No. 14 (poultry feed )

2. Beuta Clore 600 /Lit 6 Lit

3. Boron fertilizer

4. Car tap 1700 Kg /bag 7 bags

5. Cefotaxime Sodium (Claforan injection) C-380, 1g 50

6. Chlorpyriphos 1300/Lit' 2 Lit

7. Cloth bags 12"x18" (50 bags) and 18"x36" (50 bags)

8. DAP 3000 Kg/bag 5 bags

9. Karate 900/Lit 5 Lit

10. Mono hydrochloride 600 /bag 7 bags

11. Mud thiolate

12. Potash 3000 Kg/bag 5 bags

13. Potting material

14. Roundup/Glyphosate 900/Lit 5 Lit

15. Sun Star 700/pk 6 pak

16. Urea 1800 Kg/bag 5 bags

17. Used polypropylene bags Size (50 Kg, 30 Kg & 20 Kg) 50 bags each

18. Zinc sulphate 1000 Kg/bag 7 bags

Note:- A soft copy of the financial quotation should also be provided on a W/RW CD along with your quotation, please.

The end user shall examine the products delivered by the selected vendor immediately on arrival. Any defect in the products observed immediately or discernible later

upto four weeks post-delivery shall be returned and vendor will be obliged to provide a defect free replacement. The packaging should contain a label showing shelf life

(minimum 1 year at the time of delivery) along with catalogue number, lot number and molar mass/empirical formula. The vendor must be an authorized dealer of the

quoted product (certificate in this regard with current year’s warranty to be provided at the time of bidding).


Recommended