LIST OF CONTENTS

SEPTEMBER

PROCEEDINGS OF THE FOURTH INTERNATIONAL CONGRESS ON HORMONES AND CANCER

Preface vii

Acknewledgemen~ viii

W. Wels, I. M. Harwerth, N. E. Hynes and B. Groner

O~ogeaes Diminution of antibodies directed against tumor cell surface epitopes: a single chain Fv fusion molecule specifically recognizes the extracellular domain of the c-erbl~2 receptor

Roel Nmse 9 The Wnt gene family in tumorigenesis and in normal development

E. M. J. J. Berm, J. A. Foekem, W. L. J. van Patten, I. L. van Staveren, H. Portengen, W. C. H. de Koning and Jan G. M. Kllja

13 Prognostic factors in human primary breast cancer: comparison of c-myc and HER2/neu amplification

M. De Bortoli, C. Daft, S. Antoniotft, P. Magglora and M. L. Sapei

J. C. Reubi, J. Laissae, E. Krenning and S. W. J. Lamberts

21 Hormonal regulation ofc-erbB-2 oncogene expression in breast cancer cells

Receptors 27 Somatostatin receptors in human cancer: incidence, characteristics,

functional correlates and clinical implications

Jorge A. Simental, Madhabmmda Sar 37 Domain functions of the androgen receptor and Elizabeth M. Wilson

S. Stoney Simons Jr, Hlsajl Oshima 43 Modulation of the agonist activity of antisteroids by a novel cis-acting and Danlele Szapary element

S. G. A. Koehorst, H. M. Jaeobs, M. G. J. Tfianm, A. G. M. Bouwem, J. H. H. Thijssen and M. A. Blankeustein

57 Aberrant oestrogen receptor species in human meningioma tissue

A. C. B. Cato, H. K6nig, H. Ponta and P. Herdich

Robert B. Dickson, Michael D. Jolmmn, Mozeena Bano, Eric Shi, Jmiclfl Kmebayashi, Barbara Zlff, lmbel Marftnez-Lacad, Laufey T. Amandadottir and Mare E. Llppman

63 Steroids and growth promoting factors in the regulation of expression of genes and gene networks

G r o w t h C m U r o l

69 Growth factors in breast cancer: mitogenesis to transformation

Timothy C. 2~nompmn, Shin Egawa, Dov Kadmon, Gary J. Miller, Terry L. Timme, Peter T. Seardino and Sang Hee Park

79 Androgen sensitivity and gene expression in ras + myc-induced mouse prostate carcinomas

J.-Ph. Peyrat, J. Bonneterre, H. H ~ B. Heeqnet, A. Aden~ M. M. ~ , J. Lefebvre, B. Boilly and A. Demallle

87 Basic fibroblast growth factor (bFGF): mitogenic activity and binding sites in human breast cancer

Ill

IV List of Contents

liana Stancovski, Ellor Peles, Rachel Ben Levy, Raphael Lemprecht, Zvi Kelman, Rina Goldman-Michael, Esther Hurwitz, Sarah Bacas, Michael Sela and Ymef Yarden

Gy. Ktri, A. Balogh, A. Horvith, I. Mez6, Zs. Vadlsz, Gy. l~k6nyi, T. Bajor, T. V/rotes, i. Teplln, J. Horvith, O. Csuka and R. I. Nicholson

95 Signal transduction by the neu/erbB-2 receptor: a potential target for anti-tumor therapy

105 Novel antitumor peptide hormones and their effect on signal transduction

Bart van der Burg, Roll P. de Groot, Linda lsb~.ker, Wiebe Knfljer and Siegfried W. de Laat

L. C. Murphy, H. Dotzlaw, M. Alkhalaf, A. Coutts, T. Miller, M. S. J. Wong, Y. Gong and L. J. Murphy

T. J. Martin and V. Grill

Socrates E. Papaponlos and AngeUque T. M. van Holten- Verzantvoort

E. di Salle, G. Omati, D. Gindici, M. Lasses, T. R. J. Evans and R. C. Coombes

111 Direct stimulation by estrogen of growth factor signal transduction path- ways in human breast cancer cells

117 Mechanisms of growth inhibition by antiestrogens and progestins in human breast and endometrial cancer cells

Calcium

123 Hypercalcemia in cancer

131 Modulation of turnout-induced bone resorption by bisphosphonates

Enzyme lnldbitors

137 Exemestane (FCE 24304), a new steroidal aromatasc inhibitor

R. C. Coomhes 145 4-Hydroxyandrostencdione treatment for postmenopausal patients with breast cancer

V. H. T. James, M. J. Reed and A. Pm-ohit

149 Inhibition of oestrogen synthesis in postmenopausal women with breast cancer

M. DowseR, R. C. Stein and R. C. Coomhes

Geoffrey Falkson, Johann I. R u t s and Hendr~ C. Falkson

M. A. Blankenstein, I. Maittmu-Smeele, G. H. Donker, J. Daroszewski, A. Mllewicz and J. H. H. Thljssen

Alan E. Wakeling and Jean Bowler

W. H. Gispea, F. P. T. Hamers, C. J. Vecht, F. G. I. Jennekens and J. P. Neyt

S. W. J. Lambert& J.-C. Reohi and E. P. Krenning

E. Furman, E. Rmhkin, R. Margalit, P. Bendel and H. Degani

R. De Coster, W. Wouters, R. Van Ginckel, D. End, M. Krekels, M.-C. Coene and C. Bowden

155

161

Aromatization inhibition alone or in combination with GnRH agonists for the treatment of premenopausal breast cancer patients

Fadrozole hydrochloride, a new nontoxic aromatasc inhibitor for the treatment of patients with metastatic breast cancer

167 Tissue androgens and the endocrine autonomy of breast cancer

New Clinical Approaches

173 ICl 182,780, a new antioestrogen with clinical potential

179 ACTH/MSH like pcptides in the treatment of cisplatin neuropathy

185

189

197

Somatostatin receptor imaging in the diagnosis and treatment of neuroendo- crine tumors

Tamoxifen induced changes in MCF7 human breast cancer: in vitro and in vivo studies using nuclear magnetic resonance spectroscopy and imaging

Experimental studies with liarozole (R 75 251): an antitumoral agent which inhibits retinoic acid breakdown

Horst Michnn, Svante Gohring, Wolfgang Kiilmel, yukiddge Nishino and Martin R. Schneider

203 The antitumor potency of progesterone antagonists is due to their differen- tiation potential

J. G. M. Klljn, P. M. J. J. Betas, M. Bontmbal, J. Alexleva-Fignsch and J. A. Foekens

D. Chtlbes, C. Joyeux, F. Gtltier and H. Rnchefort

Ruth Lupu, Robert B. Diekson and Marc E. Lippman

Jan T6rnell, Bj6rn Carlsson, Per Pohjanen, Hikan Wennbo, Lars Rymo and Olle lsaksson

William L. McGuire~, Gary C. Chamness and Suzanne A. W. Fuqna

211

223

229

237

List of Contents V

Breast Cancer

Clinical breast cancer, new developments in selection and endocrine treat- ment of patients

Progestin-induced fatty acid synthetas¢ in human mammary tumors: from molecular to clinical studies

The role of erbB-2 and its ligands in growth control of malignant breast epithelium

High frequency of mammary adenocarcinomas in metallothionein promoter-human growth hormone transgenic mice created from two different strains of mice

243 Abnormal estrogen receptor in clinical breast cancer

Editorial Addresses

Steroid Nomenclature

Carolyn M. Klinge, Robert A. Bambara and RmseH Hill"

Masafemi Koga, Haruyoshi Nakao and Bunzo Sato

R. Ouali, S. Poulette, A. Penhoat and J. M. Saez

A. Wozniak, S. D. Holman and J. B. Hntchison

J. M. Gledhm, S. Barker, C. Wanless, J. P. Himon, J. R. Puddefoot, C. Panahy, A. W. Goode and G. P. Viason

Gflnter Kaufmann, Hubert Thole, Regine Kraft and Peter Atrat

Elisabeth Namaan-Reperant, Anne-Marie Cathlard and Philippe Durand

M. Poutanen, O. Mllentaesta, V. Isomaa, P. Jouppila and R. Vihko

Douglas M. Stocco

Amlers Rime, Sflg Hennim2mon, Birgltta Ask and Margarita G. Ladona

Radha B. lyer, Judith M. Bimtock, Ira S. Schwartz, Gary G. Gordon, Bernard I. Weimtein and A. Louis Southren

Lazar Z. Krsmanovi~, Mohamed A. Virmanl, Stanko S. StoJllkevt~ and Kevin J. Catt

iii

vii

249

263

271

281

289

O C T O B E R

Antiestrogen-liganded estrogen receptor interaction with estrogen respon- sive element D N A / n vitro

Effects of retinoic acid on estrogen- and thyroid hormone-induced growth in a newly established rat pituitary tumor cell line

Characterization and coupling of angiotensin-II receptor subtypes in cultured bovine adrenal fasciculata cells

In vitro potency and selectivity of the non-steroidal androgen aromatasc inhibitor CGS 16949A compared to steroidal inhibitors in the brain

Progesterone receptor induction by danazol in cultured cancer cells and the rat uterus

297

303

Steroid-l-dehydrogenasc of Rhodococcus erythropolis: purification and N-terminal amino acid sequence

Ontogenesis of cholesterol side-chain cleavage activity in the ovine adrenal during late gestation

311 Immunohistochemical detection of human estrogen receptor with region D-specific antipeptide antibodies

319

335

Further evidence that the mitochondrial proteins induced by hormone stimulation in MA-IO mouse Leydig tumor cells are involved in the acute regulation of steroidogenesis

Comparison of human fetal hepatic and adrenal cytochrome P450 activities with some major gestationai steroids and ethylmorphine as substrates

343 Purification and properties of human hepatic 3a-hydroxysteroid dehydrogenase

351 Actions of acetyl-L-carnitine on the hypothalamo-pituitary-gnnadal system in female rats

VI List of Contents

Short Communication Miguel A. Maestro, L. Cutedo,

A. Mourifio, Rob B. R o o ~ , R. Bosch and A. W. Norman

359

Book Reviews 363

Forthcoming Papers I

Announcement II

Synthesis and biological activity of the dihydrotachysterol2 metabolite 25-hydroxydihydrotachystero12

O C T O B E R

PROCEEDINGS OF THE XV MEETING OF THE INTERNATIONAL STUDY GROUP FOR STEROID HORMONES

M. Truss, G. Chnlepakis and M. Beato 365

Plenary Lecture Interplay of steroid hormone receptors and transcription factors on the mouse mammary tumor virus promoter

Xavier Bertagna

S. W. J. Lmberts, J. W. Koper and F. H. de Jong

John W. Fender

Ida E. T6th

O. A. Mdiler and K. yon Werder

Frnnco Mantero and Marco Boscaro

Glucocorticoids from Molecular Biology to Clinical Use 379 Unrestrained production of proopiomelanocortin (POMC) and its peptide

fragments by pituitary corticotroph adenomas in Cushing's disease

385 Familial and iatrogenic cortisol receptor resistance

389 Glucocorticoid receptors

395 Lipoprotein receptors and steroidogenesis in adrenoeortical cells

403 Ectopic production of ACTH and cortieotropin-releasing hormone (CRH)

409 Glucocorticoid-dependent hypertension

MaR~ Corvol, Odile Bianchard and Lydia Tugris

G. Forti, G. B. Vanneili, T. Barni, G. C. Balhnni, C. Orlando and M. Serio

Steroid-Peptide Interactions 415 Bone and cartilage responsiveness to sex steroid hormones

419 Sertoli-germ cells interactions in the human testis

V. Sutiemma, P. Rosati, C. Guerzoni, S. Marlani, F. Bellllotfl, M. Magnnnti, G. Garufl, T. Galoni and A. Fabbrini

423 Human Sertoli cells/n vitro: morphological features and androgen-binding protein secretion

V. Toscmm, R. Baklueci, P. Biuehi, R. Gugileimi, A. Manlliaatini and F. Sciarra

Eli Y. Adashi

431 Steroidal and non-steroidal factors in plasma sex hormone binding globulin regulation

439 The potential relevance of cytokines to ovarian physiology

Anabalic and Catabolic Effects of Steroids B. A. Cooke, M. C. IL Choi, G. Dkami, 445 Control of steroidogenesis in Leydig cells

M. P. Lopez-Ruiz and A. P. West

G. Pelletier, E. Dupont, J. Sham'd, 451 Ontogeny and subcellular localization of 3~-hydroxysteroid dehydrogenase V. Luu-The, A. B~lager (3~-HSD) in the human and rat adrenal, ovary and testis and F. Lobrte

F. Celotti and P. Negri Cesi 469 Anabolic steroids: a review of their effects on the muscles, of their possible mechanisms of action and of their use in athletics

Ellkpllmi K. Asem, Daniel J. SJmpann 479 and Babetta L. Marrone

Noriko Taimlumhi and 489 Theodore R. lkeitman

J. B. Adams and 499 Katbleen Seymour-Mmm

Toshiyuki Toko, Ken-ichi Matsuo, 507 Jim Shibata, Konstanty WJerzba, Mumoru Nukaemka, Setsuo Takeda, Yu~ Yamnda, Tetsuji Auto, Takahisa Hirose and Bunzo Sato

D. A. Williams, A. Purohit and 515 M. J. Reed

Celso E. G6mez-Sinchez, 523 Llssette M. Le6n and Elise P. G6mez-Sinchez

Alexander V. Sirotkin and Jozef Nitray

Malika Es-souni, Leyla C. Rmirez and Paulette Bournot

Katrin Undisz, Helmut Groh, Heinz Stopsack and Cllire H6rhold-Schabert

T. K. Kwan, D. J. H. Tralford, H. L. J. Makln~ A. I. Mallet and D. B. Cower

Om P. Sharum, Herman Adlercreutz, John D. Strandberg, Barry R. ZirHn, Donald S. Coffey and t ~ r y L. E w e +

Birte-Marie Agular, Anne Marie VJngganrd and Constance Vind

Khalil Nchoul, Marie-Claude Patrtcet, Jean-Pierre Moatti and Andr6 Revol

M. P. Hedger and G. P. Risbridger

Short Communication Michael Murray

Book Reviews

Corrigendum

Forthcoming Papers

Announcement

List of Contents VII

N O V E M B E R

Hormone stimulated steroid biosynthesis in granulosa cells studied with a fluorogenic probe for cytochrome P-450,,~

The covalent labeling of proteins by 17~-estradiol, retinoic acid, and progesterone in the human breast cancer cell fines MCF-7 and MCF-7/Adr R

Estrogen receptor and C~9-5-ene-steroid concentrations in the nuclear fraction from human breast carcinoma tissue

Interaction of DP-TAT-59, an active metabolite of new triphenylethylene- derivative (TAT-59), with estrogen receptors

529

535

543

Immunochemical specificity of placental NADPH cytochrome c (P-450) reductase in neoplastic and non-neoplastic human tissue

Biotin-hydrazide derivatives for the development of steroid enzyme-linked immunoassays

The interrelationships between nonapeptide and steroid hormones secretion by bovine granulosa cells/n vitro

18-Hydroxylation in the Y-1 adrenal cell line: response to ACTH and to culture conditions

Bioconversion of steroids by Cochliobolus lunatus--II. 11//-Hydroxylation of 17a,21-dihydroxypregna-l,4-diene-3,20-dione 17-acetate in dependence of the inducer structure

549 GC-MS studies of 16-androstenes and other C~9 steroids in human semen

557 Soy of dietary source plays a preventive role against the pathogenesis of prostatitis in rats

565 Regulation by dexamethasone of the 3~-hydroxysteroid dehydrogenase activity in adult rat Leydig cells

573 Determination of urinary cortisol with three commercial immunoassays

581

591

595

597

I

II

Effect of serum and serum lipoproteins on testosterone production by adult rat Leydig cells/n vitro

Participation of a cytochrome P450 enzyme from the 2C subfamily in progesterone 21-hydroxylation in sheep liver

ReJnhard Jbtckenberg, ThemM tlawlghorst, Angeliim Filmer, Emily P. ~tster, ]Ctrin n o ~ ~pJeJ netto and lfltm-Dieter Schulz

599

D E C E M B E R

Regulation of androgen receptor mRNA and protein level by steroid hormones in human mammary cancer cells

VIII List of Contents

T. Joensuu, A. Niemelli, T. Kmnu, S. Sulomaa, H. Alho, P. Villa, T. Ylikomi, M. Kulomaa and P. Tnohimaa

609 Inducibility of the avidin gene by progesterone is suppressed during estrogen-induced cytodifferentiation

Olaf Ortmann, Bj6rn Tilse and Gfmter Emons

619 Modulatory actions of estradiol and progesterone on phorbol ester- stimulated LH secretion from cultured rat pituitary cells

Andrze| Otyhar, Marion Grins, Hans-Hermmm IGltz and Olaf Pongs

Christian F. Holinka and Erlio Gurpide

R. W. Hartmann, G. Grfln, U. Bartz and M. Palzer

629 Magnetic DNA affinity purification of eedysteroid receptor

635

641

Growth-promoting effects of progesterone in a human endometrial cancer cell line (Ishikawa-Var I)

Evaluation of the racemate and the enantiomers of a new highly active and selective aromatase inhibitor of the aminogiutethimide type

P. Tnohimaa, M. Bliuer, T. Jiiskeltlaen, A. Itkonen, M. Lindfors, A. Mahonen, J. Paivimo, P. Vilja and P. H. Mienplii

649 Characterization of human 1,25-dihydroxyvitamin D3 receptor anti-peptide antibodies

Jean Marc Lobaccaro, Serge Lumbroso, Franfoise Carrt Pigeon, Jean-Lol~s Chanesuln, Jean-Edmond Tonblanc, Jean-Claude Job, Georges Olewulczack, Pierre Boulot and Chades Sultan

659 Prenatal prediction of androgen insensitivity syndrome using exon I polymorphism of the androgen receptor gene

Udo C. T. Oppermann, Edmund Maser, J. J. R. Hermans, Jan Koolman and Karl J. Netter

665 Homologies between enzymes involved in steroid and xenobiotic carbonyl reduction in vertebrates, invertebrates and procaryonts

Mario I. Skliar, Ricardo L. Boland, Antonio Mourino and Gabriel Tojo

677 Isolation and identification of vitamin D3, 25-hydroxyvitamin D3, 1,25-dihydroxyvitamin D 3 and 1,24,25-trihydroxyvitamin D 3 in Solanum malacoxylon incubated with rnminal fluid

R. A. Dressend6rfer, C. Kirschbanm, W. Rohde, F. Stahl and C. J. Strnsburger

683 Synthesis of a cortisol-biotin conjugate and evaluation as a tracer in an immunoassay for salivary cortisol measurement

Nobuyuki Kadohnm% Carol Yarborough, D u ~ Zlmu, Shinan Chen and Ymhio Omwa

693 Kinetic properties of aromatase mutants Pro308Phe, Asp309Asn, and Asp309Ala and their interactions with aromatase inhibitors

Ana M. Soto, Renan M. Siivla and Carlos Sonnenscbein

703 A plasma-borne specific inhibitor of the proliferation of human estrogen- sensitive breast tumor cells (estrocolyone-I)

S. M. Abel, J. L. Maggs, D. J. Back 713 Cortisol metabolism by human liver/n vitro--I. Metabolite identification and B. K. Park and inter-individual variability

Marie Bi~lkovi, R. Hampl and 721 L. Stirka

Harold G. Klemcke 725

Short Communication Robert W. Heideprlem,

Peter D. Livant, Edward J. P m ~ , Robert J. Barbuch, Manrice G. Broaddns and Martin Bard

741

Epitestosteronv--a potent competitive inhibitor of Ce:steroid side chain cleavage in the testis

Hormonal and stressor-associated changes in porcine adrenocortical cholesterol ester hydrolase activity

A simple method for the isolation of zymosterol from a sterol mutant of Saccharomyces cerevisiae

Forthcoming Papers

Announcements

I

II

A MOLECULAR

List of Contents

DECEMBER

VIEW OF STEROID METABOLISM

IX

lscad Hanukoglu 745 Introduction

Current research on steroid metabolism: transition from biochemistry to molecular-cell biology

Colin R. Jefcoate, Brian C. McNamara, lrina Artemenko and Takesid Yamazaki

Cholesterol Transfer to Mitochondria 751 Regulation of cholesterol movement to mitochondrial cytochrome P450scc

in steroid hormone synthesis

Peter F. Hall and Ghanim Aimahbobl 769 The role of the cytoskeleton in the regulation of steroidogenesis

Israel Hanugoglu

Fernand Labrie, Jacques Simard, Van Lnu-The, Almin B~langer and Georges Pelletler

Perrin C. White, Leigh Pueae, Kathleen M. Curaow, Grace Tannin and Ariel R~is/er

Enzymes of Steroid Metabolism in Steroidogenic Tissues 779 Steroidogenic enzymes: structure, function, and role in regulation of steroid

hormone biosynthesis

805 Structure, function and tissue-specific gene expression of 3~-hydroxysteroid dehydrogenase/5-ene-4-ene isomerase enzymes in class/ca/and peripheral intracrine steroidogenic tissues

827 Molecular biology of 1 l/Lhydroxylase and 1 l~-hydroxysteroid dehydro- genase enzymes

Jean-Guy LeHoux and Andr6 Tremblay

William E. Ralney, lan M. Bird and J. lan Mason

837 In vivo regulation of gene expression of enzymes controlling aldosterone synthesis in rat adrenal

847 Angiotensin-II-directed glomerulosa cell function in fetal adrenal cells

P. H. Proveaeher, Y. Tremblay, B. Btlanger and A. B&langer

Virginia H. Black

F. Hatey, J. P. Gasparoax, P. Mulsant, A. Bonnet and F. Gasser

A. Amsterdam, I. Hannkoglu, B. S. Sah, I. Keren-Tai, D. Plehn-Dujowkh, R. Sprengel, H. Reanert and J. F. Straml HI

855

863

869

875

Sybille G. E. Meyer 885

A. H. Payne, G. L. Yoengblood, L. Sha, M. Barges-Trinidnd and S. H. Hammond

895

Dale B. Hales, Yuthag Xiong and 907 ilmn Tur-Kaslm

Diane S. Keeney and J. lan Mason 915

Steroidogenesis in guinea pig adrenal cortex: effects of ACTH on steroid secretion and steroidogenic enzyme activities and expression

Cytochrome P450s in the guinea pig adrenal that are immunologic.ally similar to liver forms: estrogen suppression explains male-female differences

P450scc regulation in pig 8ranulosa cells: investigation into the mechanism of induction

Oncogene-transformed granulosa cells as a model system for the study of steroidogenic processes

Adrenergic influence on progesterone metabolism and cyclicity in the rat ovary: autotransplantation and chemical sympathectomy

Hormonal regulation of steroidogenic enzyme gene expression in Leydig cells

The role of cytokines in the regulation of Leydig cell P450c17 gene expression

Regulation of expression of 3p-hydroxysteroid dehydrogenase is mediated by cAMP in rat Leydig cells and H540 rat Leydig tumor cells

Molecular Analysis Evan R. SimlmOn, Michael W. Kilgore, 923

Mala S. Mahendroo, Gary D. Meam, C. Jo Corbin and Carole R. Meadelson

of Steroidogemc Enzyme Geme Transcription Regulation of human aromatase cytochrome P450 gene expression

X

Michael R. Waterman, Norio Kagawa, Ulrich M. Zanger, Kyoko Momnl, Johan Land and Evan R. Simpson

Bernard P. Schimmer and Keith L. Parker

Peter J. Hornsby, Charles Y. Cheng, Deepak S. Lala, Sepehr Steve Maghsendleu, Satyanarayana G. Rain and Lianqing Yang

931

937

951

List of Contents

Comparison of cAMP-responsive DNA sequences and their binding proteins associated with expression of the bovine CYPl7 and CYPllA and human CYP21B genes

Promoter elements of the mouse 21-hydroxylase (Cyp-21) gene involved in cell-selective and cAMP-dependent gene expression

Changes in gene expression during senescence of adrenocortical cells in culture

Molecular Genetics of Inborn Errors of Steroid Biosynthesis Walter L. Miller, 961 Analysis of the duplicated human CA/P450c21/X gene cluster

Stephen E. Gitelman, James Bristow and Yves Morel

To~hihiko yana~ , Tsuneo Imai, Evan R. Simpson and Michael R. Waterman

973 Molecular basis of 17a-hydroxylase/17,20-lyase deficiency

Yutaka Shizuta, Takeshi Kawamoto, Yasuhiro Mltsuuchi, Kateuml Toda, Kanrn Miyahara, Yoshiynki Ichikawa, Ellroo Imurn and Stanley Ulick

981 Molecular genetic studies on the biosynthesis of aldosterone in humans

Ariel R6sler 989 Steroid 17~-hydroxysteroid dehydrogenase deficiency in man: an inherited form of male pseudohermaphroditism

H. Vanden Boesche 1003 Inhibitors of P450-dependent steroid biosynthesis: from research to medical treatment

Enzymes of Steroid Metabolism in Liver and Other Organs John B. Scbonkm,u 1023 Steroid metabolism by constitutive cytochromes P450

MLuhiko Negishi, Mauhiko lwasaki, 1031 Alteration of the substrate specificity of mouse 2A P450s by the identity of Risto O. Juvonen and Kanrn A i d a residue-209: steroid-binding site and orientation

N. l-hnioka, F. J. Gunzalez, N. A. IAndborg, G. Liu and K. R. Korzekwa

1037 Chimeric eDNA expression and site directed mutagenesis studies of cyto- chrome P450s CYP2AI and CYP2A2

Stefan Westin, Petra Toilet, Andere Str6m, Agneta Mode and Jan-~kke Gustafeson

1045 The role and mechanism of growth hormone in the regulation of sexually dimorphic P450 enzymes in rat liver

David J. Waxman 1055 Regulation of liver-specific steroid metabolizing cytochromes P450: cholesterol 7=-hydroxylase, bile acid 6~8-hydroxylase, and growth hormone- responsive steroid hormone hydroxylases

H. James Armbrecht, Kyuichiro Okuda, N. Wonpm~wat, Rama K. Nemanl, Ming L. C'hea and Monlca A. Boitz

1073 Characterization and regulation of the vitamin D hydroxylases

Kuo-C'ni Cheng 1083 Molecular cloning of rat liver 3~-hydroxysteroid dehydrogenase and identification of structurally related proteins from rat lung and kidney

Jerzy A ~ , Bettina Hmea, 1089 The membrane-bound 17~8-estradiol dehydrogenase of porcine endometrial Frank Marks and Peter W. Jmtqflut ceils: purification, characterization and subceliular localization

Lulu Dnlla Valle, Panla Belvedere, Claudia SimontacchJ and Lorenzo Colombo

1095 Extraglandular hormonal steroidogenesis in aged rats

Peter I. Mackenzie, Lonles Rodboarne 1099 Steroid UDP glucuronosyltransferases and Steven Strank~

List of Contents XI

Jehn C. Leper Eazymes o f Steroid Metabollsm in Microorganisms

1107 Cytochrome P450 lanosterol 14~-demethylase (CYP51): insights from molecular genetic analysis of the ERG 11 gene in Saccharomyces cerevisiae

c , ~ - R ~ J ~ Dasmr l~d, M s r ~ M i f - F ~ m e , Heike Rimter, MedNb~ Hmalag, Waltmm Scl~mze sad Khms Rucklmul

1117 Steroid I 1,8-hydroxylation by a fungal microsomal cytochrome P450

SmMII 43~.-AA