Locating the Repressor Binding Sites in the

Bacteriophages

• Presented by Sonia Sharma

• BNFO 301

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Repressors What are They

Bacteriophages when infecting the Bacteria have to make an important

decision

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If Life is bestowed

• Lytic promoters have to be silenced• Repressors come to limelight to do

their job• They Block the promoter’s sites for

lytic pathway then proceed to Lysogeny

• Repressor also binds to DNA at specific sequences

• Found multiple times in genome

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Objective is to locate those sites

• By Utilizing• computational tools integrated in BioBIKE,-• A graphical programming interface that enables

biologist to approach complex research questions • Upstream sequences of relevant phages

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Data- available for Che12, e-lambda,L5

and Bxb1

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Same method for L5 and Bxb1

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Testing Time• Apply the same technique to find to

binding sites of some random phages

• Packman• Shaka

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Concluding thoughtResults yet to be

analyzed